ID   ROGF7_ARATH             Reviewed;         546 AA.
AC   Q9LZN0;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   22-JUL-2015, entry version 77.
DE   RecName: Full=Rop guanine nucleotide exchange factor 7;
DE            Short=AtRopGEF7;
DE   AltName: Full=Rho of plants guanine nucleotide exchange factor 7;
GN   Name=ROPGEF7; OrderedLocusNames=At5g02010; ORFNames=T7H20.60;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RL   Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY.
RX   PubMed=15980860; DOI=10.1038/nature03883;
RA   Berken A., Thomas C., Wittinghofer A.;
RT   "A new family of RhoGEFs activates the Rop molecular switch in
RT   plants.";
RL   Nature 436:1176-1180(2005).
RN   [6]
RP   FUNCTION, INTERACTION WITH ARAC1/ROP3; PLT1 AND PLT2, DEVELOPMENTAL
RP   STAGE, AND INDUCTION BY AUXIN.
RX   PubMed=21828289; DOI=10.1105/tpc.111.085514;
RA   Chen M., Liu H., Kong J., Yang Y., Zhang N., Li R., Yue J., Huang J.,
RA   Li C., Cheung A.Y., Tao L.Z.;
RT   "RopGEF7 regulates PLETHORA-dependent maintenance of the root stem
RT   cell niche in Arabidopsis.";
RL   Plant Cell 23:2880-2894(2011).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=22984069; DOI=10.1126/science.1222597;
RA   Oda Y., Fukuda H.;
RT   "Initiation of cell wall pattern by a Rho- and microtubule-driven
RT   symmetry breaking.";
RL   Science 337:1333-1336(2012).
CC   -!- FUNCTION: Guanine-nucleotide exchange factor (GEF) that acts as an
CC       activator of Rop (Rho of plants) GTPases by promoting the exchange
CC       of GDP for GTP. In postembryonic roots, modulates root stem cell
CC       maintenance by regulating the expression of PLT1 and PLT2, which
CC       are key transcription factors that mediate the patterning of the
CC       root stem cell niche. May connect RopGEF-regulated Rac/Rop
CC       signaling and auxin-dependent PLT-regulated root pattern
CC       formation. {ECO:0000269|PubMed:21828289}.
CC   -!- SUBUNIT: Interacts with ARAC1/ROP3, PLT1 and PLT2.
CC       {ECO:0000269|PubMed:21828289}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22984069}.
CC       Cell membrane {ECO:0000269|PubMed:22984069}. Note=Interacts with
CC       ARAC1/ROP3 on plasma membrane.
CC   -!- DEVELOPMENTAL STAGE: During embryogenesis, specifically expressed
CC       in the precursor cells of the quiescent center (QC) at the heart,
CC       torpedo, bent-cotyledon, and mature embryo stages. At the seedling
CC       stage, expressed in the QC and vascular bundles of root
CC       differentiated zones and lateral root primordia. Expressed in the
CC       vasculature of hypocotyls, and meristemoids and guard cells of
CC       cotyledons. {ECO:0000269|PubMed:21828289}.
CC   -!- INDUCTION: By auxin. {ECO:0000269|PubMed:21828289}.
CC   -!- DOMAIN: The PRONE (plant-specific Rop nucleotide exchanger) domain
CC       is responsible for the GEF activity. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Plants silencing ROPGEF7 show defects in embryo
CC       patterning and maintenance of the quiescent center (QC), and
CC       postembryonic loss of root stem cell population.
CC       {ECO:0000305|PubMed:22984069}.
CC   -!- SIMILARITY: Contains 1 PRONE domain. {ECO:0000255|PROSITE-
CC       ProRule:PRU00663}.
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DR   EMBL; AL162508; CAB82974.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90419.1; -; Genomic_DNA.
DR   EMBL; BT015831; AAU94394.1; -; mRNA.
DR   EMBL; BT020213; AAV59279.1; -; mRNA.
DR   PIR; T48222; T48222.
DR   RefSeq; NP_195821.1; NM_120279.2.
DR   UniGene; At.50417; -.
DR   ProteinModelPortal; Q9LZN0; -.
DR   SMR; Q9LZN0; 70-432.
DR   DIP; DIP-59396N; -.
DR   STRING; 3702.AT5G02010.1; -.
DR   PRIDE; Q9LZN0; -.
DR   EnsemblPlants; AT5G02010.1; AT5G02010.1; AT5G02010.
DR   GeneID; 831858; -.
DR   KEGG; ath:AT5G02010; -.
DR   TAIR; AT5G02010; -.
DR   eggNOG; NOG294244; -.
DR   HOGENOM; HOG000238290; -.
DR   InParanoid; Q9LZN0; -.
DR   OMA; ASEMEMM; -.
DR   PhylomeDB; Q9LZN0; -.
DR   PRO; PR:Q9LZN0; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   GO; GO:0005829; C:cytosol; IDA:TAIR.
DR   GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR   GO; GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; ISS:TAIR.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; ISS:GOC.
DR   InterPro; IPR005512; PRONE_dom.
DR   Pfam; PF03759; PRONE; 1.
DR   PROSITE; PS51334; PRONE; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Complete proteome; Cytoplasm;
KW   Guanine-nucleotide releasing factor; Membrane; Reference proteome.
FT   CHAIN         1    546       Rop guanine nucleotide exchange factor 7.
FT                                /FTId=PRO_0000423893.
FT   DOMAIN       61    440       PRONE. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00663}.
FT   COMPBIAS    518    524       Poly-Ser.
SQ   SEQUENCE   546 AA;  61753 MW;  FDA3B16C01DE041E CRC64;
     MDGSSENLPE VEEKGRESSC CSSETTRQEE EEQSPSCTED FTASPVSSRW SVKNIDGEKK
     KIRSDSRVSE VEMMKERFSK LLLGEDMSGS GNGVCTALAI SNAITNLCAT LFGQLWRLEP
     LPTEKKEMWR REMEWLLCVS DHIVEMTPTW QTFPDGTKLE IMTCRPRSDL YVNLPALRKL
     DNMLLEILDS FEETEFWYVD QGIMAHESAA DGSSSFRKSF QRQEDKWWLP VPRVSPGGLQ
     ENSRKQLQHK RDCTNQILKA AMAINSITLA DMEIPESYLE SLPRKGRSCL GDLIYRYISS
     DQFSPECLLD CLDLSSEHQA IEIANRVESS IYLWHKRTNS KPATNTKTSW EMVKELMVDA
     DKLELMADRA ESLLLSLKQR FPGLPQTALD MSKIQYNKDI GKSILESYSR VLESLAFNIV
     ARIDDLLFVD DLTRHSSDQI PTTLGNNGND APKSIAVPVS NYTTPSYSPS KQELRSSITV
     PPSPSRFKIP HSSSVKRVLT AYVTKNEPRL KNLPLERSSR SSSSERLSLE KCMKESLNVS
     NLDPGI
//