ID ROGF7_ARATH Reviewed; 546 AA. AC Q9LZN0; DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2000, sequence version 1. DT 12-AUG-2020, entry version 103. DE RecName: Full=Rop guanine nucleotide exchange factor 7; DE Short=AtRopGEF7; DE AltName: Full=Rho of plants guanine nucleotide exchange factor 7; GN Name=ROPGEF7; OrderedLocusNames=At5g02010; ORFNames=T7H20.60; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130714; DOI=10.1038/35048507; RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K., RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., RA Bevan M., Fransz P.F.; RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."; RL Nature 408:823-826(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.; RT "Arabidopsis ORF clones."; RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.; RT "Arabidopsis ORF clones."; RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases. RN [5] RP GENE FAMILY. RX PubMed=15980860; DOI=10.1038/nature03883; RA Berken A., Thomas C., Wittinghofer A.; RT "A new family of RhoGEFs activates the Rop molecular switch in plants."; RL Nature 436:1176-1180(2005). RN [6] RP FUNCTION, INTERACTION WITH ARAC1/ROP3; PLT1 AND PLT2, DEVELOPMENTAL STAGE, RP AND INDUCTION BY AUXIN. RX PubMed=21828289; DOI=10.1105/tpc.111.085514; RA Chen M., Liu H., Kong J., Yang Y., Zhang N., Li R., Yue J., Huang J., RA Li C., Cheung A.Y., Tao L.Z.; RT "RopGEF7 regulates PLETHORA-dependent maintenance of the root stem cell RT niche in Arabidopsis."; RL Plant Cell 23:2880-2894(2011). RN [7] RP SUBCELLULAR LOCATION. RX PubMed=22984069; DOI=10.1126/science.1222597; RA Oda Y., Fukuda H.; RT "Initiation of cell wall pattern by a Rho- and microtubule-driven symmetry RT breaking."; RL Science 337:1333-1336(2012). CC -!- FUNCTION: Guanine-nucleotide exchange factor (GEF) that acts as an CC activator of Rop (Rho of plants) GTPases by promoting the exchange of CC GDP for GTP. In postembryonic roots, modulates root stem cell CC maintenance by regulating the expression of PLT1 and PLT2, which are CC key transcription factors that mediate the patterning of the root stem CC cell niche. May connect RopGEF-regulated Rac/Rop signaling and auxin- CC dependent PLT-regulated root pattern formation. CC {ECO:0000269|PubMed:21828289}. CC -!- SUBUNIT: Interacts with ARAC1/ROP3, PLT1 and PLT2. CC {ECO:0000269|PubMed:21828289}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22984069}. Cell CC membrane {ECO:0000269|PubMed:22984069}. Note=Interacts with ARAC1/ROP3 CC on plasma membrane. CC -!- DEVELOPMENTAL STAGE: During embryogenesis, specifically expressed in CC the precursor cells of the quiescent center (QC) at the heart, torpedo, CC bent-cotyledon, and mature embryo stages. At the seedling stage, CC expressed in the QC and vascular bundles of root differentiated zones CC and lateral root primordia. Expressed in the vasculature of hypocotyls, CC and meristemoids and guard cells of cotyledons. CC {ECO:0000269|PubMed:21828289}. CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:21828289}. CC -!- DOMAIN: The PRONE (plant-specific Rop nucleotide exchanger) domain is CC responsible for the GEF activity. {ECO:0000250}. CC -!- MISCELLANEOUS: Plants silencing ROPGEF7 show defects in embryo CC patterning and maintenance of the quiescent center (QC), and CC postembryonic loss of root stem cell population. CC {ECO:0000305|PubMed:22984069}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL162508; CAB82974.1; -; Genomic_DNA. DR EMBL; CP002688; AED90419.1; -; Genomic_DNA. DR EMBL; BT015831; AAU94394.1; -; mRNA. DR EMBL; BT020213; AAV59279.1; -; mRNA. DR PIR; T48222; T48222. DR RefSeq; NP_195821.1; NM_120279.3. DR SMR; Q9LZN0; -. DR DIP; DIP-59396N; -. DR IntAct; Q9LZN0; 1. DR STRING; 3702.AT5G02010.1; -. DR iPTMnet; Q9LZN0; -. DR PaxDb; Q9LZN0; -. DR ProteomicsDB; 228189; -. DR EnsemblPlants; AT5G02010.1; AT5G02010.1; AT5G02010. DR GeneID; 831858; -. DR Gramene; AT5G02010.1; AT5G02010.1; AT5G02010. DR KEGG; ath:AT5G02010; -. DR Araport; AT5G02010; -. DR TAIR; locus:2185153; AT5G02010. DR eggNOG; ENOG502QPIY; Eukaryota. DR HOGENOM; CLU_019073_2_1_1; -. DR InParanoid; Q9LZN0; -. DR OMA; SIYLWHK; -. DR OrthoDB; 383472at2759; -. DR PhylomeDB; Q9LZN0; -. DR PRO; PR:Q9LZN0; -. DR Proteomes; UP000006548; Chromosome 5. DR ExpressionAtlas; Q9LZN0; baseline and differential. DR Genevisible; Q9LZN0; AT. DR GO; GO:0005829; C:cytosol; IDA:TAIR. DR GO; GO:0005886; C:plasma membrane; IDA:TAIR. DR GO; GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; ISS:TAIR. DR InterPro; IPR005512; PRONE_dom. DR InterPro; IPR038937; RopGEF. DR PANTHER; PTHR33101; PTHR33101; 1. DR Pfam; PF03759; PRONE; 1. DR PROSITE; PS51334; PRONE; 1. PE 1: Evidence at protein level; KW Cell membrane; Cytoplasm; Guanine-nucleotide releasing factor; Membrane; KW Reference proteome. FT CHAIN 1..546 FT /note="Rop guanine nucleotide exchange factor 7" FT /id="PRO_0000423893" FT DOMAIN 61..440 FT /note="PRONE" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00663" FT COMPBIAS 518..524 FT /note="Poly-Ser" SQ SEQUENCE 546 AA; 61753 MW; FDA3B16C01DE041E CRC64; MDGSSENLPE VEEKGRESSC CSSETTRQEE EEQSPSCTED FTASPVSSRW SVKNIDGEKK KIRSDSRVSE VEMMKERFSK LLLGEDMSGS GNGVCTALAI SNAITNLCAT LFGQLWRLEP LPTEKKEMWR REMEWLLCVS DHIVEMTPTW QTFPDGTKLE IMTCRPRSDL YVNLPALRKL DNMLLEILDS FEETEFWYVD QGIMAHESAA DGSSSFRKSF QRQEDKWWLP VPRVSPGGLQ ENSRKQLQHK RDCTNQILKA AMAINSITLA DMEIPESYLE SLPRKGRSCL GDLIYRYISS DQFSPECLLD CLDLSSEHQA IEIANRVESS IYLWHKRTNS KPATNTKTSW EMVKELMVDA DKLELMADRA ESLLLSLKQR FPGLPQTALD MSKIQYNKDI GKSILESYSR VLESLAFNIV ARIDDLLFVD DLTRHSSDQI PTTLGNNGND APKSIAVPVS NYTTPSYSPS KQELRSSITV PPSPSRFKIP HSSSVKRVLT AYVTKNEPRL KNLPLERSSR SSSSERLSLE KCMKESLNVS NLDPGI //