ID U72E2_ARATH Reviewed; 481 AA. AC Q9LVR1; Q8LEG2; DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2000, sequence version 1. DT 31-MAY-2011, entry version 58. DE RecName: Full=UDP-glycosyltransferase 72E2; DE EC=2.4.1.-; DE AltName: Full=Hydroxycinnamate 4-beta-glucosyltransferase; DE EC=2.4.1.126; GN Name=UGT72E2; OrderedLocusNames=At5g66690; ORFNames=MSN2.8; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX MEDLINE=20181125; PubMed=10718197; DOI=10.1093/dnares/7.1.31; RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., RA Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC RT clones."; RL DNA Res. 7:31-63(2000). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [4] RP GENE FAMILY. RX PubMed=11042215; DOI=10.1074/jbc.M007447200; RA Li Y., Baldauf S., Lim E.K., Bowles D.J.; RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family RT of Arabidopsis thaliana."; RL J. Biol. Chem. 276:4338-4343(2001). RN [5] RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES. RX PubMed=11042211; DOI=10.1074/jbc.M007263200; RA Lim E.K., Li Y., Parr A., Jackson R., Ashford D.A., Bowles D.J.; RT "Identification of glucosyltransferase genes involved in sinapate RT metabolism and lignin synthesis in Arabidopsis."; RL J. Biol. Chem. 276:4344-4349(2001). RN [6] RP FUNCTION. RX PubMed=12721858; DOI=10.1007/s00425-002-0969-0; RA Messner B., Thulke O., Schaeffner A.R.; RT "Arabidopsis glucosyltransferases with activities toward both RT endogenous and xenobiotic substrates."; RL Planta 217:138-146(2003). RN [7] RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES. RX PubMed=15907484; DOI=10.1016/j.febslet.2005.04.016; RA Lim E.K., Jackson R.G., Bowles D.J.; RT "Identification and characterisation of Arabidopsis RT glycosyltransferases capable of glucosylating coniferyl aldehyde and RT sinapyl aldehyde."; RL FEBS Lett. 579:2802-2806(2005). RN [8] RP FUNCTION, AND TISSUE SPECIFICITY. RX PubMed=16995900; DOI=10.1111/j.1365-313X.2006.02872.x; RA Lanot A., Hodge D., Jackson R.G., George G.L., Elias L., Lim E.K., RA Vaistij F.E., Bowles D.J.; RT "The glucosyltransferase UGT72E2 is responsible for monolignol 4-O- RT glucoside production in Arabidopsis thaliana."; RL Plant J. 48:286-295(2006). RN [9] RP FUNCTION. RX PubMed=21149736; DOI=10.1073/pnas.1007747108; RA Miao Y.C., Liu C.J.; RT "ATP-binding cassette-like transporters are involved in the transport RT of lignin precursors across plasma and vacuolar membranes."; RL Proc. Natl. Acad. Sci. U.S.A. 107:22728-22733(2010). CC -!- FUNCTION: Involved in the O-glucosylation of monolignols (alcohol CC monomers of lignin). Glucosylates conyferyl and sinapyl alcohols CC to coniferyl alcohol 4-O-glucoside and sinapyl alcohol 4-O- CC glucoside, respectively. Glucosylates conyferyl and sinapyl CC aldehydes. Possesses low activity with sinapate and ferulate as CC substrates. CC -!- CATALYTIC ACTIVITY: UDP-glucose + trans-4-hydroxycinnamate = UDP + CC 4-O-beta-D-glucosyl-4-hydroxycinnamate. CC -!- BIOPHYSICOCHEMICAL PROPERTIES: CC Kinetic parameters: CC KM=20 uM for conyferyl aldehyde; CC KM=20 uM for sinapyl aldehyde; CC KM=60 uM for conyferyl alcohol; CC KM=150 uM for sinapyl alcohol; CC KM=450 uM for ferulate; CC KM=900 uM for sinapate; CC -!- TISSUE SPECIFICITY: Expressed in seedlings and roots. CC -!- MISCELLANEOUS: Plant over-expressing UGT72E2 show increased CC accumulation of monolignol glucosides in roots and appearance of CC these glucosides in leaves. CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family. CC -!- SEQUENCE CAUTION: CC Sequence=AAM62659.1; Type=Erroneous initiation; Note=Translation N-terminally extended; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB018119; BAA97275.1; -; Genomic_DNA. DR EMBL; AY062636; AAL32714.1; -; mRNA. DR EMBL; AY064651; AAL47362.1; -; mRNA. DR EMBL; AY085432; AAM62659.1; ALT_INIT; mRNA. DR IPI; IPI00540555; -. DR RefSeq; NP_201470.1; NM_126067.2. DR UniGene; At.27462; -. DR ProteinModelPortal; Q9LVR1; -. DR SMR; Q9LVR1; 5-474. DR CAZy; GT1; Glycosyltransferase Family 1. DR PRIDE; Q9LVR1; -. DR EnsemblPlants; AT5G66690.1; AT5G66690.1; AT5G66690. DR GeneID; 836802; -. DR KEGG; ath:AT5G66690; -. DR NMPDR; fig|3702.1.peg.28774; -. DR TAIR; At5g66690; -. DR GeneTree; EPGT00050000005352; -. DR InParanoid; Q9LVR1; -. DR ProtClustDB; PLN02992; -. DR Genevestigator; Q9LVR1; -. DR GO; GO:0047209; F:coniferyl-alcohol glucosyltransferase activity; IDA:TAIR. DR GO; GO:0009808; P:lignin metabolic process; TAS:TAIR. DR InterPro; IPR002213; UDP_glucos_trans. DR PANTHER; PTHR11926; UDP_glucos_trans; 1. DR Pfam; PF00201; UDPGT; 1. DR PROSITE; PS00375; UDPGT; 1. PE 1: Evidence at protein level; KW Complete proteome; Glycosyltransferase; Transferase. FT CHAIN 1 481 UDP-glycosyltransferase 72E2. FT /FTId=PRO_0000409074. FT REGION 346 348 UDP-glucose binding (By similarity). FT REGION 363 371 UDP-glucose binding (By similarity). FT REGION 385 388 UDP-glucose binding (By similarity). FT BINDING 272 272 UDP-glucose (By similarity). FT CONFLICT 3 3 I -> N (in Ref. 3; AAM62659). SQ SEQUENCE 481 AA; 52992 MW; EE46B76C4BD14745 CRC64; MHITKPHAAM FSSPGMGHVI PVIELGKRLS ANNGFHVTVF VLETDAASAQ SKFLNSTGVD IVKLPSPDIY GLVDPDDHVV TKIGVIMRAA VPALRSKIAA MHQKPTALIV DLFGTDALCL AKEFNMLSYV FIPTNARFLG VSIYYPNLDK DIKEEHTVQR NPLAIPGCEP VRFEDTLDAY LVPDEPVYRD FVRHGLAYPK ADGILVNTWE EMEPKSLKSL LNPKLLGRVA RVPVYPIGPL CRPIQSSETD HPVLDWLNEQ PNESVLYISF GSGGCLSAKQ LTELAWGLEQ SQQRFVWVVR PPVDGSCCSE YVSANGGGTE DNTPEYLPEG FVSRTSDRGF VVPSWAPQAE ILSHRAVGGF LTHCGWSSTL ESVVGGVPMI AWPLFAEQNM NAALLSDELG IAVRLDDPKE DISRWKIEAL VRKVMTEKEG EAMRRKVKKL RDSAEMSLSI DGGGLAHESL CRVTKECQRF LERVVDLSRG A //