ID TR105_MOUSE Reviewed; 300 AA. AC Q9JKT4; DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2000, sequence version 1. DT 26-FEB-2020, entry version 121. DE RecName: Full=Taste receptor type 2 member 105; DE Short=T2R105; DE AltName: Full=Taste receptor type 2 member 5; DE Short=T2R5; DE AltName: Full=Taste receptor type 2 member 9; DE Short=T2R9; GN Name=Tas2r105; Synonyms=Tas2r5, Tas2r9; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TOPOLOGY. RC STRAIN=129/SvJ; RX PubMed=10761934; DOI=10.1016/s0092-8674(00)80705-9; RA Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P., RA Zuker C.S.; RT "A novel family of mammalian taste receptors."; RL Cell 100:693-702(2000). RN [2] RP FUNCTION, VARIANTS ILE-44; ASP-155 AND ARG-294, AND POLYMORPHISM. RX PubMed=10761935; DOI=10.1016/s0092-8674(00)80706-0; RA Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W., RA Zuker C.S., Ryba N.J.P.; RT "T2Rs function as bitter taste receptors."; RL Cell 100:703-711(2000). RN [3] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=15759003; DOI=10.1038/nature03352; RA Mueller K.L., Hoon M.A., Erlenbach I., Chandrashekar J., Zuker C.S., RA Ryba N.J.; RT "The receptors and coding logic for bitter taste."; RL Nature 434:225-229(2005). RN [4] RP REVIEW. RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8; RA Montmayeur J.-P., Matsunami H.; RT "Receptors for bitter and sweet taste."; RL Curr. Opin. Neurobiol. 12:366-371(2002). RN [5] RP REVIEW. RX PubMed=11696554; DOI=10.1074/jbc.r100054200; RA Margolskee R.F.; RT "Molecular mechanisms of bitter and sweet taste transduction."; RL J. Biol. Chem. 277:1-4(2002). RN [6] RP REVIEW. RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0; RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., RA Zuker C.S., Ryba N.J.; RT "Coding of sweet, bitter, and umami tastes: different receptor cells RT sharing similar signaling pathways."; RL Cell 112:293-301(2003). CC -!- FUNCTION: Gustducin-coupled cycloheximide receptor implicated in the CC perception of bitter compounds in the oral cavity and the CC gastrointestinal tract. Signals through PLCB2 and the calcium-regulated CC cation channel TRPM5. {ECO:0000269|PubMed:10761935, CC ECO:0000269|PubMed:15759003}. CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the CC tongue and palate epithelium and exclusively in gustducin-positive CC cells. Expressed in gastric and duodenal tissues. CC -!- POLYMORPHISM: Variations in Tas2r105 are associated with avoidance of CC cycloheximide at low concentrations. {ECO:0000269|PubMed:10761935}. CC -!- DISRUPTION PHENOTYPE: Mice fail to avoid cycloheximide at low CC concentrations and show a lack of cycloheximide-induced action CC potentials in a principal nerve innervating taste receptor cells of the CC tongue. {ECO:0000269|PubMed:15759003}. CC -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single CC taste receptor cell. CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF227147; AAF43920.1; -; Genomic_DNA. DR CCDS; CCDS20612.1; -. DR RefSeq; NP_065247.1; NM_020501.1. DR STRING; 10090.ENSMUSP00000058006; -. DR ChEMBL; CHEMBL3714703; -. DR PaxDb; Q9JKT4; -. DR PRIDE; Q9JKT4; -. DR Ensembl; ENSMUST00000053652; ENSMUSP00000058006; ENSMUSG00000051153. DR GeneID; 57252; -. DR KEGG; mmu:57252; -. DR UCSC; uc009eiz.1; mouse. DR CTD; 57252; -. DR MGI; MGI:2681195; Tas2r105. DR eggNOG; ENOG410IMNH; Eukaryota. DR eggNOG; ENOG410Z4AM; LUCA. DR GeneTree; ENSGT00960000186648; -. DR HOGENOM; CLU_072337_3_0_1; -. DR InParanoid; Q9JKT4; -. DR KO; K08474; -. DR OMA; IANYSHC; -. DR OrthoDB; 1010573at2759; -. DR PhylomeDB; Q9JKT4; -. DR TreeFam; TF335891; -. DR PRO; PR:Q9JKT4; -. DR Proteomes; UP000000589; Chromosome 6. DR RNAct; Q9JKT4; protein. DR Bgee; ENSMUSG00000051153; Expressed in fibroblast and 16 other tissues. DR ExpressionAtlas; Q9JKT4; differential. DR Genevisible; Q9JKT4; MM. DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central. DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW. DR GO; GO:0008527; F:taste receptor activity; TAS:UniProtKB. DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IBA:GO_Central. DR GO; GO:0050913; P:sensory perception of bitter taste; IMP:MGI. DR InterPro; IPR007960; T2R. DR Pfam; PF05296; TAS2R; 1. PE 1: Evidence at protein level; KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor; KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane; KW Transmembrane helix. FT CHAIN 1..300 FT /note="Taste receptor type 2 member 105" FT /id="PRO_0000082359" FT TOPO_DOM 1..7 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 8..28 FT /note="Helical; Name=1" FT /evidence="ECO:0000255" FT TOPO_DOM 29..43 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 44..64 FT /note="Helical; Name=2" FT /evidence="ECO:0000255" FT TOPO_DOM 65..87 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 88..108 FT /note="Helical; Name=3" FT /evidence="ECO:0000255" FT TOPO_DOM 109..128 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 129..149 FT /note="Helical; Name=4" FT /evidence="ECO:0000255" FT TOPO_DOM 150..181 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 182..202 FT /note="Helical; Name=5" FT /evidence="ECO:0000255" FT TOPO_DOM 203..233 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 234..254 FT /note="Helical; Name=6" FT /evidence="ECO:0000255" FT TOPO_DOM 255..259 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 260..280 FT /note="Helical; Name=7" FT /evidence="ECO:0000255" FT TOPO_DOM 281..300 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT CARBOHYD 161 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT VARIANT 44 FT /note="T -> I (in strain: BALB/c, C3H/He, CBA/Ca and DBA/ FT 2J)" FT /evidence="ECO:0000269|PubMed:10761935" FT VARIANT 155 FT /note="G -> D (in strain: BALB/c, C3H/He, CBA/Ca and DBA/ FT 2J)" FT /evidence="ECO:0000269|PubMed:10761935" FT VARIANT 294 FT /note="L -> R (in strain: BALB/c, C3H/He, CBA/Ca and DBA/ FT 2J)" FT /evidence="ECO:0000269|PubMed:10761935" SQ SEQUENCE 300 AA; 34416 MW; 92AD449C68FB85EB CRC64; MLSAAEGILL SIATVEAGLG VLGNTFIALV NCMDWAKNNK LSMTGFLLIG LATSRIFIVW LLTLDAYAKL FYPSKYFSSS LIEIISYIWM TVNHLTVWFA TSLSIFYFLK IANFSDCVFL WLKRRTDKAF VFLLGCLLTS WVISFSFVVK VMKDGKVNHR NRTSEMYWEK RQFTINYVFL NIGVISLFMM TLTACFLLIM SLWRHSRQMQ SGVSGFRDLN TEAHVKAIKF LISFIILFVL YFIGVSIEII CIFIPENKLL FIFGFTTASI YPCCHSFILI LSNSQLKQAF VKVLQGLKFF //