ID DP3A_PSEAE STANDARD; PRT; 1173 AA. AC Q9HXZ1; Q9F662; DT 16-OCT-2001 (Rel. 40, Created) DT 16-OCT-2001 (Rel. 40, Last sequence update) DT 16-OCT-2001 (Rel. 40, Last annotation update) DE DNA polymerase III alpha subunit (EC 2.7.7.7). GN DNAE OR PA3640. OS Pseudomonas aeruginosa. OC Bacteria; Proteobacteria; gamma subdivision; Pseudomonadaceae; OC Pseudomonas. OX NCBI_TaxID=287; RN [1] RP SEQUENCE FROM N.A. RC STRAIN=ATCC 15692 / PAO1; RX MEDLINE=20437337; PubMed=10984043; RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M., RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., RA Reizer J., Saier M.H., Hancock R.E.W., Lory S., Olson M.V.; RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an RT opportunistic pathogen."; RL Nature 406:959-964(2000). RN [2] RP SEQUENCE FROM N.A. RA Oliver A., Baquero F., Blazquez J.; RT "Naturally-ocurring dnaE Pseudomonas aeruginosa mutations."; RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: DNA POLYMERASE III IS A COMPLEX, MULTICHAIN ENZYME CC RESPONSIBLE FOR MOST OF THE REPLICATIVE SYNTHESIS IN BACTERIA. CC THIS DNA POLYMERASE ALSO EXHIBITS 3' TO 5' EXONUCLEASE ACTIVITY. CC THE ALPHA CHAIN IS THE DNA POLYMERASE (BY SIMILARITY). CC -!- CATALYTIC ACTIVITY: N DEOXYNUCLEOSIDE TRIPHOSPHATE = CC N PYROPHOSPHATE + DNA(N). CC -!- SUBUNIT: CONTAINS A CORE (COMPOSED OF ALPHA, EPSILON, AND THETA CC CHAINS) THAT ASSOCIATES WITH A TAU SUBUNIT WHICH ALLOW THE CORE CC DIMERIZATION TO FORM THE POLIII' COMPLEX. POLIII' ASSOCIATES WITH CC THE GAMMA COMPLEX (COMPOSED OF CHAINS GAMMA, DELTA, DELTA', PSI, CC AND CHI) AND WITH THE BETA CHAIN (BY SIMILARITY). CC -!- SUBCELLULAR LOCATION: CYTOPLASMIC (BY SIMILARITY). CC -!- SIMILARITY: BELONGS TO DNA POLYMERASE TYPE-C FAMILY. DNAE CC SUBFAMILY. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE004784; AAG07028.1; -. DR EMBL; AF297647; AAG16710.2; -. DR InterPro; IPR004013; PHP_C. DR InterPro; IPR003141; PHP_N. DR InterPro; IPR002309; tRNA-synt_2. DR Pfam; PF02811; PHP_C; 1. DR Pfam; PF02231; PHP_N; 1. DR Pfam; PF01336; tRNA_anti; 1. DR SMART; SM00481; POLIIIAc; 1. KW Transferase; DNA-directed DNA polymerase; DNA replication; KW Complete proteome. SQ SEQUENCE 1173 AA; 130903 MW; AE3DFE97D82EB3B0 CRC64; MTVSFVHLRL HTEFSLVDGL VRVKPLAKAV AGLGMPAVAV TDQSNMCSLV KFYKTAMGAG IKPICGADIW LASREEDGPL SRLSLLAMNA KGYRNLTELI SRGWSEGQRN GEIIIERDWV KEAAEGLIAL SAAKEGEIGH ALLDGEEAKA EALLREWMEV FPERFYVEVQ RTSRVNDEEH LHAAVALASR CNAPLVATND VRFIKQEDFE AHETRVCIGE GRTLDDPRRP RTYSDQQYLK SPAEMAELFS DLPEALENTV EIAKRCNIEV QLGKYFLPDF PTPNGMGIDD YLRHASFEGL EERLEVLLPK DTPDYEAKRQ VYVDRLNFEL DIIIQMGFPG YFLIVMDFIK WAKNNGVPVG PGRGSGAGSL VAYVLKITDL DPLAYDLLFE RFLNPERISM PDFDVDFCME GRDRVIDYVA DAYGRNAVSQ IITFGTMAAK AVVRDVARVQ GKSYGLADRL SKMIPFEVGM TLDKAYEQEE MLRDFLKSDE EAAEIWEMAL KLEGITRGTG KHAGGVVIAP TKLTDFSPIA CDEEGGGLVT QFDKDDVEAA GLVKFDFLGL RTLTIIKWAM EIINREQAKK GLEPVNIDFI PLDDKPTYSL LQKAETTAVF QLESRGMKEL IKKLKPDCLE DLIALVALFR PGPLQSGMVD DFINRKHGRA ELSYPHPDYQ YAGLEPVLKP TYGIILYQEQ VMQIAQVMAG YTLGGADMLR RAMGKKKPEE MAKQRGGFIE GCKNNGIDAD LAGNIFDLVE KFAGYGFNKS HSAAYGLVSY QTAWLKTHFP APFMAAVLTA DMQNTDKVVT LIEECRHMKL RIVAPDVNNS EFRFTVDDDG RIVYGLGAIK GVGEGPVEAI TECRAEGGPF NTLFDFCDRV DLKRINKRTL EALIRAGALD RLGPHYHDEL KAYQATVDLN RAVLLAAMEE AIQAAEQTAR SHDSGHMDLF GGVFAEPEAD VYANHRKVKE LTLKERLKGE KDTLGLYLTG HPIDEYEGEV RRFARQRIVE LKPARDTQTV AGLIVNLRVM KNKKGDKMGF VTLDDRSGRI EASLFSEAFA AAQSLLQTDA LVVVEGEVSQ DDFSGGLRLR AKRVMSLEEA RTGLAESLRM KLHADLLKGD RLRWLGELFN RHRGSCPITL DYTSADAKAL LQFGESWRVD PADDLIQALR DQFGRDNVFL NYR //