ID DPO3A_PSEAE Reviewed; 1173 AA. AC Q9HXZ1; Q9F662; DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2001, sequence version 1. DT 03-MAY-2023, entry version 121. DE RecName: Full=DNA polymerase III subunit alpha; DE EC=2.7.7.7; GN Name=dnaE; OrderedLocusNames=PA3640; OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1). OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=208964; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / RC PRS 101 / PAO1; RX PubMed=10984043; DOI=10.1038/35023079; RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M., RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.; RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic RT pathogen."; RL Nature 406:959-964(2000). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RA Oliver A., Baquero F., Blazquez J.; RT "Naturally-ocurring dnaE Pseudomonas aeruginosa mutations."; RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme CC responsible for most of the replicative synthesis in bacteria. This DNA CC polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain CC is the DNA polymerase (By similarity). {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, CC ChEBI:CHEBI:173112; EC=2.7.7.7; CC -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon CC and theta chains) that associates with a tau subunit. This core CC dimerizes to form the PolIII' complex. PolIII' associates with the CC gamma complex (composed of gamma, delta, delta', psi and chi chains) CC and with the beta chain to form the complete DNA polymerase III complex CC (By similarity). {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE CC subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE004091; AAG07028.1; -; Genomic_DNA. DR EMBL; AF297647; AAG16710.2; -; Genomic_DNA. DR PIR; H83189; H83189. DR RefSeq; NP_252330.1; NC_002516.2. DR RefSeq; WP_003098578.1; NZ_QZGE01000001.1. DR AlphaFoldDB; Q9HXZ1; -. DR SMR; Q9HXZ1; -. DR STRING; 287.DR97_4299; -. DR PaxDb; Q9HXZ1; -. DR EnsemblBacteria; AAG07028; AAG07028; PA3640. DR GeneID; 880490; -. DR KEGG; pae:PA3640; -. DR PATRIC; fig|208964.12.peg.3809; -. DR PseudoCAP; PA3640; -. DR HOGENOM; CLU_001600_0_0_6; -. DR InParanoid; Q9HXZ1; -. DR OMA; NECRRMG; -. DR OrthoDB; 9803237at2; -. DR PhylomeDB; Q9HXZ1; -. DR BioCyc; PAER208964:G1FZ6-3710-MON; -. DR Proteomes; UP000002438; Chromosome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro. DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW. DR CDD; cd04485; DnaE_OBF; 1. DR CDD; cd07433; PHP_PolIIIA_DnaE1; 1. DR Gene3D; 1.10.150.870; -; 1. DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1. DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1. DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1. DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom. DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom. DR InterPro; IPR040982; DNA_pol3_finger. DR InterPro; IPR029460; DNAPol_HHH. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR004365; NA-bd_OB_tRNA. DR InterPro; IPR004013; PHP_dom. DR InterPro; IPR003141; Pol/His_phosphatase_N. DR InterPro; IPR016195; Pol/histidinol_Pase-like. DR InterPro; IPR004805; PolC_alpha. DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1. DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1. DR Pfam; PF07733; DNA_pol3_alpha; 1. DR Pfam; PF17657; DNA_pol3_finger; 1. DR Pfam; PF14579; HHH_6; 1. DR Pfam; PF02811; PHP; 1. DR Pfam; PF01336; tRNA_anti-codon; 1. DR SMART; SM00481; POLIIIAc; 1. DR SUPFAM; SSF89550; PHP domain-like; 1. DR TIGRFAMs; TIGR00594; polc; 1. PE 3: Inferred from homology; KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; KW Nucleotidyltransferase; Reference proteome; Transferase. FT CHAIN 1..1173 FT /note="DNA polymerase III subunit alpha" FT /id="PRO_0000103334" SQ SEQUENCE 1173 AA; 130905 MW; AE3DFE97D82EB3B0 CRC64; MTVSFVHLRL HTEFSLVDGL VRVKPLAKAV AGLGMPAVAV TDQSNMCSLV KFYKTAMGAG IKPICGADIW LASREEDGPL SRLSLLAMNA KGYRNLTELI SRGWSEGQRN GEIIIERDWV KEAAEGLIAL SAAKEGEIGH ALLDGEEAKA EALLREWMEV FPERFYVEVQ RTSRVNDEEH LHAAVALASR CNAPLVATND VRFIKQEDFE AHETRVCIGE GRTLDDPRRP RTYSDQQYLK SPAEMAELFS DLPEALENTV EIAKRCNIEV QLGKYFLPDF PTPNGMGIDD YLRHASFEGL EERLEVLLPK DTPDYEAKRQ VYVDRLNFEL DIIIQMGFPG YFLIVMDFIK WAKNNGVPVG PGRGSGAGSL VAYVLKITDL DPLAYDLLFE RFLNPERISM PDFDVDFCME GRDRVIDYVA DAYGRNAVSQ IITFGTMAAK AVVRDVARVQ GKSYGLADRL SKMIPFEVGM TLDKAYEQEE MLRDFLKSDE EAAEIWEMAL KLEGITRGTG KHAGGVVIAP TKLTDFSPIA CDEEGGGLVT QFDKDDVEAA GLVKFDFLGL RTLTIIKWAM EIINREQAKK GLEPVNIDFI PLDDKPTYSL LQKAETTAVF QLESRGMKEL IKKLKPDCLE DLIALVALFR PGPLQSGMVD DFINRKHGRA ELSYPHPDYQ YAGLEPVLKP TYGIILYQEQ VMQIAQVMAG YTLGGADMLR RAMGKKKPEE MAKQRGGFIE GCKNNGIDAD LAGNIFDLVE KFAGYGFNKS HSAAYGLVSY QTAWLKTHFP APFMAAVLTA DMQNTDKVVT LIEECRHMKL RIVAPDVNNS EFRFTVDDDG RIVYGLGAIK GVGEGPVEAI TECRAEGGPF NTLFDFCDRV DLKRINKRTL EALIRAGALD RLGPHYHDEL KAYQATVDLN RAVLLAAMEE AIQAAEQTAR SHDSGHMDLF GGVFAEPEAD VYANHRKVKE LTLKERLKGE KDTLGLYLTG HPIDEYEGEV RRFARQRIVE LKPARDTQTV AGLIVNLRVM KNKKGDKMGF VTLDDRSGRI EASLFSEAFA AAQSLLQTDA LVVVEGEVSQ DDFSGGLRLR AKRVMSLEEA RTGLAESLRM KLHADLLKGD RLRWLGELFN RHRGSCPITL DYTSADAKAL LQFGESWRVD PADDLIQALR DQFGRDNVFL NYR //