ID PREB_HUMAN Reviewed; 417 AA. AC Q9HCU5; Q53SZ8; Q9UH94; DT 24-OCT-2001, integrated into UniProtKB/Swiss-Prot. DT 23-NOV-2004, sequence version 2. DT 20-JAN-2016, entry version 135. DE RecName: Full=Prolactin regulatory element-binding protein; DE AltName: Full=Mammalian guanine nucleotide exchange factor mSec12; GN Name=PREB; Synonyms=SEC12; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND TISSUE SPECIFICITY. RX PubMed=10920239; DOI=10.1007/s003350010142; RA Taylor Clelland C.L., Levy B., McKie J.M., Duncan A.M.V., RA Hirschhorn K., Bancroft C.; RT "Cloning and characterization of human PREB; a gene that maps to a RT genomic region associated with trisomy 2p syndrome."; RL Mamm. Genome 11:675-681(2000). RN [2] RP NUCLEOTIDE SEQUENCE. RA Edgar A.J., Polak J.M.; RT "The human and mouse homologues of rat prolactin regulatory element RT binding protein."; RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY. RX PubMed=12735795; DOI=10.1186/1471-2164-4-18; RA Edgar A.J.; RT "The gene structure and expression of human ABHD1: overlapping RT polyadenylation signal sequence with Sec12."; RL BMC Genomics 4:18-18(2003). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15815621; DOI=10.1038/nature03466; RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J., RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., RA Waterston R.H., Wilson R.K.; RT "Generation and annotation of the DNA sequences of human chromosomes 2 RT and 4."; RL Nature 434:724-731(2005). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., RA Venter J.C.; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Brain, Lung, and Uterus; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [8] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21269460; DOI=10.1186/1752-0509-5-17; RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.; RT "Initial characterization of the human central proteome."; RL BMC Syst. Biol. 5:17-17(2011). RN [9] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Liver; RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014; RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., RA Wang L., Ye M., Zou H.; RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human RT liver phosphoproteome."; RL J. Proteomics 96:253-262(2014). CC -!- FUNCTION: Was first identified based on its probable role in the CC regulation of pituitary gene transcription. Binds to the prolactin CC gene (PRL) promoter and seems to activate transcription (By CC similarity). Guanine nucleotide exchange factor that activates CC SARA2. Required for the formation of COPII transport vesicles from CC the ER. {ECO:0000250}. CC -!- SUBUNIT: Binds SARA2. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Endoplasmic reticulum CC membrane {ECO:0000250}; Single-pass membrane protein CC {ECO:0000250}. CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:10920239, CC ECO:0000269|PubMed:12735795}. CC -!- SIMILARITY: Contains 7 WD repeats. {ECO:0000255|PROSITE- CC ProRule:PRU00221}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF226684; AAG01692.1; -; mRNA. DR EMBL; AF203687; AAF19192.1; -; mRNA. DR EMBL; AF227166; AAF74572.1; -; Genomic_DNA. DR EMBL; AK023064; BAB14385.1; -; mRNA. DR EMBL; AC013403; AAX93170.1; -; Genomic_DNA. DR EMBL; CH471053; EAX00627.1; -; Genomic_DNA. DR EMBL; BC002765; AAH02765.1; -; mRNA. DR EMBL; BC012890; AAH12890.1; -; mRNA. DR EMBL; BC016472; AAH16472.1; -; mRNA. DR EMBL; BC041032; AAH41032.1; -; mRNA. DR CCDS; CCDS1738.1; -. DR RefSeq; NP_037520.1; NM_013388.4. DR UniGene; Hs.279784; -. DR ProteinModelPortal; Q9HCU5; -. DR SMR; Q9HCU5; 152-222. DR BioGrid; 115419; 8. DR IntAct; Q9HCU5; 12. DR MINT; MINT-4539832; -. DR STRING; 9606.ENSP00000260643; -. DR iPTMnet; Q9HCU5; -. DR PhosphoSite; Q9HCU5; -. DR BioMuta; PREB; -. DR DMDM; 55977881; -. DR MaxQB; Q9HCU5; -. DR PaxDb; Q9HCU5; -. DR PeptideAtlas; Q9HCU5; -. DR PRIDE; Q9HCU5; -. DR DNASU; 10113; -. DR Ensembl; ENST00000260643; ENSP00000260643; ENSG00000138073. DR GeneID; 10113; -. DR KEGG; hsa:10113; -. DR UCSC; uc002rix.1; human. DR CTD; 10113; -. DR GeneCards; PREB; -. DR HGNC; HGNC:9356; PREB. DR HPA; HPA013582; -. DR HPA; HPA014133; -. DR MIM; 606395; gene. DR neXtProt; NX_Q9HCU5; -. DR PharmGKB; PA33727; -. DR eggNOG; KOG0771; Eukaryota. DR eggNOG; ENOG410XRQK; LUCA. DR GeneTree; ENSGT00390000018031; -. DR HOGENOM; HOG000045302; -. DR HOVERGEN; HBG002027; -. DR InParanoid; Q9HCU5; -. DR KO; K14003; -. DR OMA; AHSMFVT; -. DR OrthoDB; EOG7XM305; -. DR PhylomeDB; Q9HCU5; -. DR TreeFam; TF314383; -. DR Reactome; R-HSA-204005; COPII (Coat Protein 2) Mediated Vesicle Transport. DR Reactome; R-HSA-381038; XBP1(S) activates chaperone genes. DR Reactome; R-HSA-5694530; Cargo concentration in the ER. DR SignaLink; Q9HCU5; -. DR ChiTaRS; PREB; human. DR GeneWiki; PREB; -. DR GenomeRNAi; 10113; -. DR NextBio; 38259; -. DR PRO; PR:Q9HCU5; -. DR Proteomes; UP000005640; Chromosome 2. DR Bgee; Q9HCU5; -. DR CleanEx; HS_PREB; -. DR ExpressionAtlas; Q9HCU5; baseline and differential. DR Genevisible; Q9HCU5; HS. DR GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome. DR GO; GO:0000139; C:Golgi membrane; TAS:GOC. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0016020; C:membrane; IDA:UniProtKB. DR GO; GO:0005634; C:nucleus; TAS:ProtInc. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0044267; P:cellular protein metabolic process; TAS:Reactome. DR GO; GO:0048208; P:COPII vesicle coating; TAS:Reactome. DR GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; TAS:Reactome. DR GO; GO:0006888; P:ER to Golgi vesicle-mediated transport; TAS:Reactome. DR GO; GO:0036498; P:IRE1-mediated unfolded protein response; TAS:Reactome. DR GO; GO:0061024; P:membrane organization; TAS:Reactome. DR GO; GO:0043687; P:post-translational protein modification; TAS:Reactome. DR GO; GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome. DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW. DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:ProtInc. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW. DR Gene3D; 2.130.10.10; -; 1. DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam. DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom. DR InterPro; IPR001680; WD40_repeat. DR InterPro; IPR017986; WD40_repeat_dom. DR Pfam; PF00400; WD40; 1. DR SMART; SM00320; WD40; 4. DR SUPFAM; SSF50998; SSF50998; 2. DR PROSITE; PS50082; WD_REPEATS_2; 1. DR PROSITE; PS50294; WD_REPEATS_REGION; 1. PE 1: Evidence at protein level; KW Activator; Complete proteome; DNA-binding; Endoplasmic reticulum; KW ER-Golgi transport; Membrane; Nitration; Nucleus; Protein transport; KW Reference proteome; Repeat; Transcription; Transcription regulation; KW Transmembrane; Transmembrane helix; Transport; WD repeat. FT CHAIN 1 417 Prolactin regulatory element-binding FT protein. FT /FTId=PRO_0000051154. FT TOPO_DOM 1 388 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 389 409 Helical. {ECO:0000255}. FT TOPO_DOM 410 417 Lumenal. {ECO:0000255}. FT REPEAT 4 50 WD 1. FT REPEAT 58 97 WD 2. FT REPEAT 127 183 WD 3. FT REPEAT 187 224 WD 4. FT REPEAT 230 287 WD 5. FT REPEAT 291 329 WD 6. FT REPEAT 334 385 WD 7. FT MOD_RES 10 10 Nitrated tyrosine. FT {ECO:0000250|UniProtKB:Q9WUQ2}. FT CONFLICT 19 19 L -> F (in Ref. 1; AAG01692). FT {ECO:0000305}. SQ SEQUENCE 417 AA; 45468 MW; 7FD490A641202D19 CRC64; MGRRRAPELY RAPFPLYALQ VDPSTGLLIA AGGGGAAKTG IKNGVHFLQL ELINGRLSAS LLHSHDTETR ATMNLALAGD ILAAGQDAHC QLLRFQAHQQ QGNKAEKAGS KEQGPRQRKG AAPAEKKCGA ETQHEGLELR VENLQAVQTD FSSDPLQKVV CFNHDNTLLA TGGTDGYVRV WKVPSLEKVL EFKAHEGEIE DLALGPDGKL VTVGRDLKAS VWQKDQLVTQ LHWQENGPTF SSTPYRYQAC RFGQVPDQPA GLRLFTVQIP HKRLRQPPPC YLTAWDGSNF LPLRTKSCGH EVVSCLDVSE SGTFLGLGTV TGSVAIYIAF SLQCLYYVRE AHGIVVTDVA FLPEKGRGPE LLGSHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG LIIVTILLLQ SAFPGFL //