ID   BRF2_HUMAN              Reviewed;         419 AA.
AC   Q9HAW0; B2RD62; Q9H2Y3; Q9H3B3; Q9NUY6;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   11-JAN-2011, entry version 82.
DE   RecName: Full=Transcription factor IIIB 50 kDa subunit;
DE            Short=TFIIIB50;
DE            Short=hTFIIIB50;
DE   AltName: Full=B-related factor 2;
DE            Short=BRF-2;
DE   AltName: Full=hBRFU;
GN   Name=BRF2; Synonyms=BRFU; ORFNames=PRO1470;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   MEDLINE=20496900; PubMed=11040218; DOI=10.1101/gad.836400;
RA   Schramm L., Pendergrast P.S., Sun Y., Hernandez N.;
RT   "Different human TFIIIB activities direct RNA polymerase III
RT   transcription from TATA-containing and TATA-less promoters.";
RL   Genes Dev. 14:2650-2663(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   MEDLINE=20570456; PubMed=11121026; DOI=10.1073/pnas.97.26.14200;
RA   Teichmann M., Wang Z., Roeder R.G.;
RT   "A stable complex of a novel transcription factor IIB- related factor,
RT   human TFIIIB50, and associated proteins mediate selective
RT   transcription by RNA polymerase III of genes with upstream promoter
RT   elements.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:14200-14205(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta, and Umbilical cord blood;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 264-419.
RC   TISSUE=Fetal liver;
RX   MEDLINE=21376145; PubMed=11483580; DOI=10.1101/gr.175501;
RA   Yu Y., Zhang C., Zhou G., Wu S., Qu X., Wei H., Xing G., Dong C.,
RA   Zhai Y., Wan J., Ouyang S., Li L., Zhang S., Zhou K., Zhang Y., Wu C.,
RA   He F.;
RT   "Gene expression profiling in human fetal liver and identification of
RT   tissue- and developmental-stage-specific genes through compiled
RT   expression profiles and efficient cloning of full-length cDNAs.";
RL   Genome Res. 11:1392-1403(2001).
RN   [7]
RP   FUNCTION, AND INTERACTION WITH TBP.
RX   PubMed=11564744; DOI=10.1074/jbc.M108515200;
RA   Cabart P., Murphy S.;
RT   "BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of
RT   polymerase III small nuclear RNA gene promoters through its
RT   interaction with TATA-binding protein.";
RL   J. Biol. Chem. 276:43056-43064(2001).
RN   [8]
RP   IDENTIFICATION IN THE TFIIIB-ALPHA COMPLEX.
RX   PubMed=12016223; DOI=10.1074/jbc.M203119200;
RA   Cabart P., Murphy S.;
RT   "Assembly of human small nuclear RNA gene-specific transcription
RT   factor IIIB complex de novo on and off promoter.";
RL   J. Biol. Chem. 277:26831-26838(2002).
RN   [9]
RP   SUBUNIT.
RX   PubMed=12391172; DOI=10.1128/MCB.22.22.8067-8078.2002;
RA   Ma B., Hernandez N.;
RT   "Redundant cooperative interactions for assembly of a human U6
RT   transcription initiation complex.";
RL   Mol. Cell. Biol. 22:8067-8078(2002).
RN   [10]
RP   INTERACTION WITH SNAPC4, AND SUBUNIT.
RX   PubMed=12621023; DOI=10.1074/jbc.M204247200;
RA   Hinkley C.S., Hirsch H.A., Gu L., LaMere B., Henry R.W.;
RT   "The small nuclear RNA-activating protein 190 Myb DNA binding domain
RT   stimulates TATA box-binding protein-TATA box recognition.";
RL   J. Biol. Chem. 278:18649-18657(2003).
RN   [11]
RP   SUBUNIT.
RX   PubMed=14527415; DOI=10.1016/j.molcel.2003.08.011;
RA   Hu P., Wu S., Hernandez N.;
RT   "A minimal RNA polymerase III transcription system from human cells
RT   reveals positive and negative regulatory roles for CK2.";
RL   Mol. Cell 12:699-709(2003).
RN   [12]
RP   SUBUNIT.
RX   PubMed=16227591; DOI=10.1128/MCB.25.21.9406-9418.2005;
RA   Saxena A., Ma B., Schramm L., Hernandez N.;
RT   "Structure-function analysis of the human TFIIB-related factor II
RT   protein reveals an essential role for the C-terminal domain in RNA
RT   polymerase III transcription.";
RL   Mol. Cell. Biol. 25:9406-9418(2005).
RN   [13]
RP   SUBUNIT.
RX   PubMed=16769183; DOI=10.1016/j.gene.2006.03.012;
RA   Emran F., Florens L., Ma B., Swanson S.K., Washburn M.P.,
RA   Hernandez N.;
RT   "A role for Yin Yang-1 (YY1) in the assembly of snRNA transcription
RT   complexes.";
RL   Gene 377:96-108(2006).
RN   [14]
RP   INTERACTION WITH MAF1, AND SUBCELLULAR LOCATION.
RX   PubMed=17505538;
RA   Rollins J., Veras I., Cabarcas S., Willis I., Schramm L.;
RT   "Human Maf1 negatively regulates RNA polymerase III transcription via
RT   the TFIIB family members Brf1 and Brf2.";
RL   Int. J. Biol. Sci. 3:292-302(2007).
RN   [15]
RP   INDUCTION.
RX   PubMed=17624304; DOI=10.1016/j.bbrc.2007.06.114;
RA   Jacob J., Cabarcas S., Veras I., Zaveri N., Schramm L.;
RT   "The green tea component EGCG inhibits RNA polymerase III
RT   transcription.";
RL   Biochem. Biophys. Res. Commun. 360:778-783(2007).
CC   -!- FUNCTION: General activator of RNA polymerase III transcription.
CC       Factor exclusively required for RNA polymerase III transcription
CC       of genes with promoter elements upstream of the initiation sites.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SUBUNIT: Component of TFIIIB complex. The TFIIIB complex has two
CC       activities, alpha and beta. The TFIIIB-alpha activity complex is
CC       composed of TBP, BDP1, and a complex containing both BRF2 and at
CC       least four stably associated proteins; this complex inhibits the
CC       transcription by pol III via its phosphorylation by CK2; YY1
CC       facilitates the TFIIIB-alpha complex formation. BRF2 recruitment
CC       to the TATA box containing small nuclear RNA (snRNA) gene
CC       templates is TBP-dependent. Interacts with TBP; this interaction
CC       with TBP mediates its TATA-box recruitment of these promoters.
CC       Interacts with TBP and the BURE sequence (GC-rich sequence
CC       downstream from the TATA box) to form a strong ternary complex
CC       which is joined by BDP1; this ternary complex stimulates pol III
CC       transcription. Forms a trimeric complex composed of TBP, BRF2 and
CC       mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of
CC       SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is
CC       stimulated by SNAP190. Interacts with MAF1 and SNAPC4.
CC   -!- INTERACTION:
CC       P28288:ABCD3; NbExp=1; IntAct=EBI-1055224, EBI-80992;
CC       O95831:AIFM1; NbExp=1; IntAct=EBI-1055224, EBI-356440;
CC       Q9NVI7:ATAD3A; NbExp=1; IntAct=EBI-1055224, EBI-352007;
CC       Q9Y678:COPG; NbExp=1; IntAct=EBI-1055224, EBI-1049127;
CC       O60762:DPM1; NbExp=1; IntAct=EBI-1055224, EBI-719526;
CC       Q9NVI1:FANCI; NbExp=1; IntAct=EBI-1055224, EBI-1013291;
CC       Q92616:GCN1L1; NbExp=1; IntAct=EBI-1055224, EBI-356221;
CC       P57678:GEMIN4; NbExp=1; IntAct=EBI-1055224, EBI-356700;
CC       Q86Y56:HEATR2; NbExp=1; IntAct=EBI-1055224, EBI-719966;
CC       Q8TEX9:IPO4; NbExp=1; IntAct=EBI-1055224, EBI-395967;
CC       P56192:MARS; NbExp=1; IntAct=EBI-1055224, EBI-355848;
CC       O43795:MYO1B; NbExp=1; IntAct=EBI-1055224, EBI-351119;
CC       Q9Y3T9:NOC2L; NbExp=1; IntAct=EBI-1055224, EBI-751547;
CC       Q8N1F7:NUP93; NbExp=1; IntAct=EBI-1055224, EBI-1042703;
CC       Q01804:OTUD4; NbExp=1; IntAct=EBI-1055224, EBI-1054396;
CC       Q9P035:PTPLAD1; NbExp=1; IntAct=EBI-1055224, EBI-359013;
CC       O75533:SF3B1; NbExp=1; IntAct=EBI-1055224, EBI-876542;
CC       P08195:SLC3A2; NbExp=1; IntAct=EBI-1055224, EBI-702356;
CC       Q9NXE4:SMPD4; NbExp=1; IntAct=EBI-1055224, EBI-372102;
CC       P57088:TMEM33; NbExp=1; IntAct=EBI-1055224, EBI-1048629;
CC       Q9Y5L0:TNPO3; NbExp=1; IntAct=EBI-1055224, EBI-1042571;
CC       Q9H3U1:UNC45A; NbExp=1; IntAct=EBI-1055224, EBI-1048763;
CC       Q9C0E2:XPO4; NbExp=1; IntAct=EBI-1055224, EBI-1044855;
CC       Q96QU8:XPO6; NbExp=1; IntAct=EBI-1055224, EBI-1022896;
CC       Q9UIA9:XPO7; NbExp=1; IntAct=EBI-1055224, EBI-286668;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- INDUCTION: Down-regulated by epigallocatechin gallate (EGCG)
CC       treatment.
CC   -!- SIMILARITY: Belongs to the TFIIB family.
CC   -!- SIMILARITY: Contains 1 TFIIB-type zinc finger.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG35486.1; Type=Erroneous initiation;
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DR   EMBL; AF298153; AAG30222.1; -; mRNA.
DR   EMBL; AF206673; AAG35669.2; -; mRNA.
DR   EMBL; AK001914; BAA91975.1; -; mRNA.
DR   EMBL; AK315420; BAG37809.1; -; mRNA.
DR   EMBL; CH471080; EAW63351.1; -; Genomic_DNA.
DR   EMBL; BC010648; AAH10648.1; -; mRNA.
DR   EMBL; AF130058; AAG35486.1; ALT_INIT; mRNA.
DR   IPI; IPI00301190; -.
DR   RefSeq; NP_060780.2; NM_018310.3.
DR   UniGene; Hs.726335; -.
DR   ProteinModelPortal; Q9HAW0; -.
DR   SMR; Q9HAW0; 1-41.
DR   IntAct; Q9HAW0; 57.
DR   STRING; Q9HAW0; -.
DR   PhosphoSite; Q9HAW0; -.
DR   PRIDE; Q9HAW0; -.
DR   Ensembl; ENST00000220659; ENSP00000220659; ENSG00000104221.
DR   GeneID; 55290; -.
DR   KEGG; hsa:55290; -.
DR   UCSC; uc003xkk.1; human.
DR   CTD; 55290; -.
DR   GeneCards; GC08M037701; -.
DR   HGNC; HGNC:17298; BRF2.
DR   HPA; CAB019269; -.
DR   HPA; HPA023378; -.
DR   MIM; 607013; gene.
DR   PharmGKB; PA164741329; -.
DR   eggNOG; prNOG09105; -.
DR   HOGENOM; HBG443627; -.
DR   HOVERGEN; HBG107537; -.
DR   InParanoid; Q9HAW0; -.
DR   OMA; WPLTMGT; -.
DR   OrthoDB; EOG9SBHHT; -.
DR   PhylomeDB; Q9HAW0; -.
DR   Reactome; REACT_1788; Transcription.
DR   NextBio; 59474; -.
DR   ArrayExpress; Q9HAW0; -.
DR   Bgee; Q9HAW0; -.
DR   CleanEx; HS_BRF2; -.
DR   Genevestigator; Q9HAW0; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0030528; F:transcription regulator activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro.
DR   GO; GO:0006352; P:transcription initiation; IEA:InterPro.
DR   InterPro; IPR006670; Cyclin.
DR   InterPro; IPR011028; Cyclin-like.
DR   InterPro; IPR013763; Cyclin-related.
DR   InterPro; IPR000812; TFIIB-related.
DR   InterPro; IPR013137; Znf_TFIIB.
DR   Gene3D; G3DSA:1.10.472.10; Cyclin_related; 1.
DR   PANTHER; PTHR11618; TFIIB_euk_relate; 1.
DR   Pfam; PF08271; TFIIB_Zn_Ribbon; 1.
DR   SMART; SM00385; CYCLIN; 1.
DR   SUPFAM; SSF47954; Cyclin_like; 1.
DR   PROSITE; PS00782; TFIIB; FALSE_NEG.
DR   PROSITE; PS51134; ZF_TFIIB; 1.
PE   1: Evidence at protein level;
KW   Activator; Complete proteome; Metal-binding; Nucleus; Repeat;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN         1    419       Transcription factor IIIB 50 kDa subunit.
FT                                /FTId=PRO_0000337187.
FT   REPEAT       72    157       1.
FT   REPEAT      173    249       2.
FT   ZN_FING       2     36       TFIIB-type.
FT   METAL         7      7       Zinc (By similarity).
FT   METAL        10     10       Zinc (By similarity).
FT   METAL        28     28       Zinc (By similarity).
FT   METAL        31     31       Zinc (By similarity).
FT   CONFLICT    153    153       Y -> C (in Ref. 3; BAA91975).
FT   CONFLICT    376    376       S -> F (in Ref. 2; AAG35669).
SQ   SEQUENCE   419 AA;  46533 MW;  90A39F72DBA14888 CRC64;
     MPGRGRCPDC GSTELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNL REVTYSRSTG
     ENEQVSRSQQ RGLRRVRDLC RVLQLPPTFE DTAVAYYQQA YRHSGIRAAR LQKKEVLVGC
     CVLITCRQHN WPLTMGAICT LLYADLDVFS STYMQIVKLL GLDVPSLCLA ELVKTYCSSF
     KLFQASPSVP AKYVEDKEKM LSRTMQLVEL ANETWLVTGR HPLPVITAAT FLAWQSLQPA
     DRLSCSLARF CKLANVDLPY PASSRLQELL AVLLRMAEQL AWLRVLRLDK RSVVKHIGDL
     LQHRQSLVRS AFRDGTAEVE TREKEPPGWG QGQGEGEVGN NSLGLPQGKR PASPALLLPP
     CMLKSPKRIC PVPPVSTVTG DENISDSEIE QYLRTPQEVR DFQRAQAARQ AATSVPNPP
//