ID   PKHF2_HUMAN             Reviewed;         249 AA.
AC   Q9H8W4;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   07-OCT-2020, entry version 163.
DE   RecName: Full=Pleckstrin homology domain-containing family F member 2;
DE            Short=PH domain-containing family F member 2;
DE   AltName: Full=Endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains;
DE            Short=EAPF;
DE   AltName: Full=PH and FYVE domain-containing protein 2;
DE   AltName: Full=Phafin-2;
DE            Short=Phafin2;
DE   AltName: Full=Zinc finger FYVE domain-containing protein 18;
GN   Name=PLEKHF2; Synonyms=ZFYVE18;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Shi H., Hong W.;
RT   "Phafin 2, PH and FYVE domain-containing protein 2.";
RL   Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Melanoma;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=B-cell;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND DOMAIN.
RX   PubMed=18288467; DOI=10.1007/s00109-007-0298-7;
RA   Li C., Liu Q., Li N., Chen W., Wang L., Wang Y., Yu Y., Cao X.;
RT   "EAPF/Phafin-2, a novel endoplasmic reticulum-associated protein,
RT   facilitates TNF-alpha-triggered cellular apoptosis through endoplasmic
RT   reticulum-mitochondrial apoptotic pathway.";
RL   J. Mol. Med. 86:471-484(2008).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-239, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [7]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-44, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19608861; DOI=10.1126/science.1175371;
RA   Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA   Olsen J.V., Mann M.;
RT   "Lysine acetylation targets protein complexes and co-regulates major
RT   cellular functions.";
RL   Science 325:834-840(2009).
RN   [8]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=19995552; DOI=10.1016/j.bbrc.2009.12.016;
RA   Lin W.J., Yang C.Y., Lin Y.C., Tsai M.C., Yang C.W., Tung C.Y., Ho P.Y.,
RA   Kao F.J., Lin C.H.;
RT   "Phafin2 modulates the structure and function of endosomes by a Rab5-
RT   dependent mechanism.";
RL   Biochem. Biophys. Res. Commun. 391:1043-1048(2010).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-239 AND SER-248, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA   Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA   Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT   "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT   site occupancy during mitosis.";
RL   Sci. Signal. 3:RA3-RA3(2010).
RN   [10]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [11]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-239 AND SER-248, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA   Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA   Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT   "System-wide temporal characterization of the proteome and phosphoproteome
RT   of human embryonic stem cell differentiation.";
RL   Sci. Signal. 4:RS3-RS3(2011).
RN   [12]
RP   FUNCTION, INTERACTION WITH EEA1, AND SUBCELLULAR LOCATION.
RX   PubMed=22816767; DOI=10.1111/j.1600-0854.2012.01400.x;
RA   Pedersen N.M., Raiborg C., Brech A., Skarpen E., Roxrud I., Platta H.W.,
RA   Liestol K., Stenmark H.;
RT   "The PtdIns3P-binding protein Phafin 2 mediates epidermal growth factor
RT   receptor degradation by promoting endosome fusion.";
RL   Traffic 13:1547-1563(2012).
RN   [13]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [14]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [15]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712; DOI=10.1002/pmic.201400617;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA   Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
CC   -!- FUNCTION: May play a role in early endosome fusion upstream of RAB5,
CC       hence regulating receptor trafficking and fluid-phase transport.
CC       Enhances cellular sensitivity to TNF-induced apoptosis
CC       (PubMed:18288467). {ECO:0000269|PubMed:18288467,
CC       ECO:0000269|PubMed:19995552, ECO:0000269|PubMed:22816767}.
CC   -!- SUBUNIT: May interact with EEA1.
CC   -!- INTERACTION:
CC       Q9H8W4; Q9H172: ABCG4; NbExp=3; IntAct=EBI-742388, EBI-8584118;
CC       Q9H8W4; Q96HD9: ACY3; NbExp=4; IntAct=EBI-742388, EBI-3916242;
CC       Q9H8W4; O75689: ADAP1; NbExp=3; IntAct=EBI-742388, EBI-714732;
CC       Q9H8W4; Q13155: AIMP2; NbExp=3; IntAct=EBI-742388, EBI-745226;
CC       Q9H8W4; A1A5B4: ANO9; NbExp=3; IntAct=EBI-742388, EBI-3843564;
CC       Q9H8W4; Q4LE39-3: ARID4B; NbExp=3; IntAct=EBI-742388, EBI-11957452;
CC       Q9H8W4; Q12797-6: ASPH; NbExp=3; IntAct=EBI-742388, EBI-12092171;
CC       Q9H8W4; Q9NY43: BARHL2; NbExp=3; IntAct=EBI-742388, EBI-10316571;
CC       Q9H8W4; O75934: BCAS2; NbExp=3; IntAct=EBI-742388, EBI-1050106;
CC       Q9H8W4; Q9H6U6: BCAS3; NbExp=3; IntAct=EBI-742388, EBI-6083685;
CC       Q9H8W4; Q9UL45: BLOC1S6; NbExp=4; IntAct=EBI-742388, EBI-465781;
CC       Q9H8W4; Q96IK1-2: BOD1; NbExp=3; IntAct=EBI-742388, EBI-18924329;
CC       Q9H8W4; Q9NPI1: BRD7; NbExp=3; IntAct=EBI-742388, EBI-711221;
CC       Q9H8W4; Q96G97-4: BSCL2; NbExp=3; IntAct=EBI-742388, EBI-10178113;
CC       Q9H8W4; Q8TAV5: C11orf45; NbExp=3; IntAct=EBI-742388, EBI-12810853;
CC       Q9H8W4; P01024: C3; NbExp=3; IntAct=EBI-742388, EBI-905851;
CC       Q9H8W4; Q9HC52: CBX8; NbExp=3; IntAct=EBI-742388, EBI-712912;
CC       Q9H8W4; Q8IYE0: CCDC146; NbExp=3; IntAct=EBI-742388, EBI-10749669;
CC       Q9H8W4; Q86WR0: CCDC25; NbExp=3; IntAct=EBI-742388, EBI-2690264;
CC       Q9H8W4; Q86Y33-5: CDC20B; NbExp=3; IntAct=EBI-742388, EBI-11983537;
CC       Q9H8W4; Q9C0F1: CEP44; NbExp=3; IntAct=EBI-742388, EBI-744115;
CC       Q9H8W4; Q8TCT0: CERK; NbExp=3; IntAct=EBI-742388, EBI-10274247;
CC       Q9H8W4; Q9Y6H1: CHCHD2; NbExp=3; IntAct=EBI-742388, EBI-2321769;
CC       Q9H8W4; Q9UKJ5: CHIC2; NbExp=3; IntAct=EBI-742388, EBI-741528;
CC       Q9H8W4; P10606: COX5B; NbExp=3; IntAct=EBI-742388, EBI-1053725;
CC       Q9H8W4; P05813: CRYBA1; NbExp=3; IntAct=EBI-742388, EBI-7043337;
CC       Q9H8W4; Q15038: DAZAP2; NbExp=3; IntAct=EBI-742388, EBI-724310;
CC       Q9H8W4; Q9NQL9: DMRT3; NbExp=3; IntAct=EBI-742388, EBI-9679045;
CC       Q9H8W4; Q92608: DOCK2; NbExp=3; IntAct=EBI-742388, EBI-448771;
CC       Q9H8W4; Q8IZD9: DOCK3; NbExp=3; IntAct=EBI-742388, EBI-1752361;
CC       Q9H8W4; Q86UW9: DTX2; NbExp=3; IntAct=EBI-742388, EBI-740376;
CC       Q9H8W4; Q13115: DUSP4; NbExp=3; IntAct=EBI-742388, EBI-6591081;
CC       Q9H8W4; Q99613: EIF3C; NbExp=3; IntAct=EBI-742388, EBI-353741;
CC       Q9H8W4; A0A0C4DGQ7: EML2; NbExp=3; IntAct=EBI-742388, EBI-12112376;
CC       Q9H8W4; Q9UPT5-1: EXOC7; NbExp=3; IntAct=EBI-742388, EBI-6251402;
CC       Q9H8W4; P16930: FAH; NbExp=3; IntAct=EBI-742388, EBI-4397076;
CC       Q9H8W4; Q9NVF7: FBXO28; NbExp=6; IntAct=EBI-742388, EBI-740282;
CC       Q9H8W4; Q969U6: FBXW5; NbExp=3; IntAct=EBI-742388, EBI-741068;
CC       Q9H8W4; Q92913: FGF13; NbExp=3; IntAct=EBI-742388, EBI-1054883;
CC       Q9H8W4; Q96C98: FHL3; NbExp=3; IntAct=EBI-742388, EBI-10229248;
CC       Q9H8W4; P21333-2: FLNA; NbExp=5; IntAct=EBI-742388, EBI-9641086;
CC       Q9H8W4; Q9BZ67: FRMD8; NbExp=8; IntAct=EBI-742388, EBI-5773072;
CC       Q9H8W4; P55040: GEM; NbExp=3; IntAct=EBI-742388, EBI-744104;
CC       Q9H8W4; P55789: GFER; NbExp=3; IntAct=EBI-742388, EBI-718281;
CC       Q9H8W4; Q86WP2: GPBP1; NbExp=3; IntAct=EBI-742388, EBI-2349758;
CC       Q9H8W4; Q6IC98: GRAMD4; NbExp=3; IntAct=EBI-742388, EBI-10962409;
CC       Q9H8W4; Q9BX10: GTPBP2; NbExp=3; IntAct=EBI-742388, EBI-6115579;
CC       Q9H8W4; Q9Y5Z4: HEBP2; NbExp=3; IntAct=EBI-742388, EBI-741593;
CC       Q9H8W4; Q9UBY9: HSPB7; NbExp=6; IntAct=EBI-742388, EBI-739361;
CC       Q9H8W4; Q9ULG1: INO80; NbExp=3; IntAct=EBI-742388, EBI-769345;
CC       Q9H8W4; O43448: KCNAB3; NbExp=3; IntAct=EBI-742388, EBI-12050557;
CC       Q9H8W4; Q587J8: KHDC3L; NbExp=3; IntAct=EBI-742388, EBI-22731520;
CC       Q9H8W4; P60014: KRTAP10-10; NbExp=3; IntAct=EBI-742388, EBI-11955579;
CC       Q9H8W4; Q6L8H1: KRTAP5-4; NbExp=3; IntAct=EBI-742388, EBI-11963072;
CC       Q9H8W4; Q96JM7: L3MBTL3; NbExp=3; IntAct=EBI-742388, EBI-2686809;
CC       Q9H8W4; Q96JM7-2: L3MBTL3; NbExp=3; IntAct=EBI-742388, EBI-11985629;
CC       Q9H8W4; O95751: LDOC1; NbExp=5; IntAct=EBI-742388, EBI-740738;
CC       Q9H8W4; Q86X45: LRRC6; NbExp=3; IntAct=EBI-742388, EBI-9379658;
CC       Q9H8W4; Q86UL8-2: MAGI2; NbExp=3; IntAct=EBI-742388, EBI-12081182;
CC       Q9H8W4; Q02750: MAP2K1; NbExp=3; IntAct=EBI-742388, EBI-492564;
CC       Q9H8W4; P61244: MAX; NbExp=4; IntAct=EBI-742388, EBI-751711;
CC       Q9H8W4; Q9NS73: MBIP; NbExp=3; IntAct=EBI-742388, EBI-741953;
CC       Q9H8W4; Q9NS73-5: MBIP; NbExp=3; IntAct=EBI-742388, EBI-10182361;
CC       Q9H8W4; P02686-2: MBP; NbExp=3; IntAct=EBI-742388, EBI-12159027;
CC       Q9H8W4; Q9HAF1: MEAF6; NbExp=5; IntAct=EBI-742388, EBI-399266;
CC       Q9H8W4; P55081: MFAP1; NbExp=3; IntAct=EBI-742388, EBI-1048159;
CC       Q9H8W4; Q504T8: MIDN; NbExp=3; IntAct=EBI-742388, EBI-7153979;
CC       Q9H8W4; Q9BU76: MMTAG2; NbExp=3; IntAct=EBI-742388, EBI-742459;
CC       Q9H8W4; A2RU80: MPP4; NbExp=3; IntAct=EBI-742388, EBI-18924629;
CC       Q9H8W4; Q9BV20: MRI1; NbExp=3; IntAct=EBI-742388, EBI-747381;
CC       Q9H8W4; Q16718: NDUFA5; NbExp=3; IntAct=EBI-742388, EBI-746417;
CC       Q9H8W4; Q12857-2: NFIA; NbExp=3; IntAct=EBI-742388, EBI-12119652;
CC       Q9H8W4; Q86SE8: NPM2; NbExp=3; IntAct=EBI-742388, EBI-6658150;
CC       Q9H8W4; Q86SE8-2: NPM2; NbExp=3; IntAct=EBI-742388, EBI-12193061;
CC       Q9H8W4; O96028: NSD2; NbExp=3; IntAct=EBI-742388, EBI-2693298;
CC       Q9H8W4; P50583: NUDT2; NbExp=3; IntAct=EBI-742388, EBI-10096247;
CC       Q9H8W4; Q99572: P2RX7; NbExp=3; IntAct=EBI-742388, EBI-1753251;
CC       Q9H8W4; Q9BY11: PACSIN1; NbExp=3; IntAct=EBI-742388, EBI-721769;
CC       Q9H8W4; Q9NWT1: PAK1IP1; NbExp=3; IntAct=EBI-742388, EBI-7641942;
CC       Q9H8W4; Q16654: PDK4; NbExp=3; IntAct=EBI-742388, EBI-2861674;
CC       Q9H8W4; Q92569: PIK3R3; NbExp=3; IntAct=EBI-742388, EBI-79893;
CC       Q9H8W4; Q96CD2: PPCDC; NbExp=3; IntAct=EBI-742388, EBI-724333;
CC       Q9H8W4; Q6NYC8: PPP1R18; NbExp=3; IntAct=EBI-742388, EBI-2557469;
CC       Q9H8W4; P54619: PRKAG1; NbExp=3; IntAct=EBI-742388, EBI-1181439;
CC       Q9H8W4; P10644: PRKAR1A; NbExp=3; IntAct=EBI-742388, EBI-476431;
CC       Q9H8W4; P26045: PTPN3; NbExp=3; IntAct=EBI-742388, EBI-1047946;
CC       Q9H8W4; Q96N64-2: PWWP2A; NbExp=3; IntAct=EBI-742388, EBI-18924849;
CC       Q9H8W4; P51157: RAB28; NbExp=3; IntAct=EBI-742388, EBI-11898753;
CC       Q9H8W4; Q5JT25-2: RAB41; NbExp=3; IntAct=EBI-742388, EBI-12315199;
CC       Q9H8W4; Q9UI14: RABAC1; NbExp=3; IntAct=EBI-742388, EBI-712367;
CC       Q9H8W4; P10276: RARA; NbExp=3; IntAct=EBI-742388, EBI-413374;
CC       Q9H8W4; Q0VAM2-3: RASGEF1B; NbExp=3; IntAct=EBI-742388, EBI-12013954;
CC       Q9H8W4; O43665: RGS10; NbExp=3; IntAct=EBI-742388, EBI-1057034;
CC       Q9H8W4; P57771-2: RGS8; NbExp=3; IntAct=EBI-742388, EBI-12058229;
CC       Q9H8W4; Q8IXW5: RPAP2; NbExp=3; IntAct=EBI-742388, EBI-395878;
CC       Q9H8W4; Q96AT9: RPE; NbExp=3; IntAct=EBI-742388, EBI-372480;
CC       Q9H8W4; Q9NS64: RPRM; NbExp=3; IntAct=EBI-742388, EBI-1052363;
CC       Q9H8W4; P63220: RPS21; NbExp=6; IntAct=EBI-742388, EBI-714051;
CC       Q9H8W4; Q6UXX9: RSPO2; NbExp=3; IntAct=EBI-742388, EBI-8481036;
CC       Q9H8W4; P23297: S100A1; NbExp=3; IntAct=EBI-742388, EBI-743686;
CC       Q9H8W4; Q99584: S100A13; NbExp=3; IntAct=EBI-742388, EBI-721909;
CC       Q9H8W4; Q96NU1: SAMD11; NbExp=3; IntAct=EBI-742388, EBI-14067109;
CC       Q9H8W4; Q96KG9-4: SCYL1; NbExp=3; IntAct=EBI-742388, EBI-12023020;
CC       Q9H8W4; Q9UDX3: SEC14L4; NbExp=3; IntAct=EBI-742388, EBI-10320311;
CC       Q9H8W4; Q15019: SEPTIN2; NbExp=3; IntAct=EBI-742388, EBI-741220;
CC       Q9H8W4; Q14141: SEPTIN6; NbExp=3; IntAct=EBI-742388, EBI-745901;
CC       Q9H8W4; Q9Y6X0: SETBP1; NbExp=3; IntAct=EBI-742388, EBI-2548259;
CC       Q9H8W4; Q9Y6X0-2: SETBP1; NbExp=6; IntAct=EBI-742388, EBI-12235818;
CC       Q9H8W4; Q16560-2: SNRNP35; NbExp=3; IntAct=EBI-742388, EBI-12938570;
CC       Q9H8W4; Q9BQB4: SOST; NbExp=3; IntAct=EBI-742388, EBI-5746563;
CC       Q9H8W4; Q86W54-2: SPATA24; NbExp=3; IntAct=EBI-742388, EBI-12041693;
CC       Q9H8W4; Q9Y4P9-2: SPEF1; NbExp=3; IntAct=EBI-742388, EBI-12303571;
CC       Q9H8W4; Q9BUD6: SPON2; NbExp=3; IntAct=EBI-742388, EBI-10298801;
CC       Q9H8W4; Q96FJ0: STAMBPL1; NbExp=3; IntAct=EBI-742388, EBI-745021;
CC       Q9H8W4; Q13188: STK3; NbExp=3; IntAct=EBI-742388, EBI-992580;
CC       Q9H8W4; Q17RD7: SYT16; NbExp=3; IntAct=EBI-742388, EBI-10238936;
CC       Q9H8W4; Q5QJ74: TBCEL; NbExp=3; IntAct=EBI-742388, EBI-10244795;
CC       Q9H8W4; O15273: TCAP; NbExp=3; IntAct=EBI-742388, EBI-954089;
CC       Q9H8W4; Q8N8B7-2: TCEANC; NbExp=3; IntAct=EBI-742388, EBI-11955057;
CC       Q9H8W4; Q9P2Z0: THAP10; NbExp=3; IntAct=EBI-742388, EBI-745404;
CC       Q9H8W4; Q9NWX6: THG1L; NbExp=3; IntAct=EBI-742388, EBI-746510;
CC       Q9H8W4; O95379: TNFAIP8; NbExp=4; IntAct=EBI-742388, EBI-1049336;
CC       Q9H8W4; Q8WVP5: TNFAIP8L1; NbExp=3; IntAct=EBI-742388, EBI-752102;
CC       Q9H8W4; Q05BL1: TP53BP2; NbExp=3; IntAct=EBI-742388, EBI-11952721;
CC       Q9H8W4; Q9Y4K3: TRAF6; NbExp=3; IntAct=EBI-742388, EBI-359276;
CC       Q9H8W4; Q8N609: TRAM1L1; NbExp=3; IntAct=EBI-742388, EBI-11996766;
CC       Q9H8W4; O43617: TRAPPC3; NbExp=3; IntAct=EBI-742388, EBI-743566;
CC       Q9H8W4; O43715: TRIAP1; NbExp=3; IntAct=EBI-742388, EBI-2820212;
CC       Q9H8W4; Q8IYM9: TRIM22; NbExp=3; IntAct=EBI-742388, EBI-954123;
CC       Q9H8W4; Q8N5M4: TTC9C; NbExp=3; IntAct=EBI-742388, EBI-2851213;
CC       Q9H8W4; Q9NX01: TXNL4B; NbExp=3; IntAct=EBI-742388, EBI-10309345;
CC       Q9H8W4; Q9H832: UBE2Z; NbExp=9; IntAct=EBI-742388, EBI-720977;
CC       Q9H8W4; Q16851: UGP2; NbExp=3; IntAct=EBI-742388, EBI-743729;
CC       Q9H8W4; P46094: XCR1; NbExp=3; IntAct=EBI-742388, EBI-12960721;
CC       Q9H8W4; Q9H0D6: XRN2; NbExp=3; IntAct=EBI-742388, EBI-372110;
CC       Q9H8W4; O95070: YIF1A; NbExp=3; IntAct=EBI-742388, EBI-2799703;
CC       Q9H8W4; P25490: YY1; NbExp=3; IntAct=EBI-742388, EBI-765538;
CC       Q9H8W4; Q15916: ZBTB6; NbExp=6; IntAct=EBI-742388, EBI-7227791;
CC       Q9H8W4; Q6UX98: ZDHHC24; NbExp=3; IntAct=EBI-742388, EBI-10254561;
CC       Q9H8W4; Q68DK2-5: ZFYVE26; NbExp=3; IntAct=EBI-742388, EBI-8656416;
CC       Q9H8W4; P49910: ZNF165; NbExp=3; IntAct=EBI-742388, EBI-741694;
CC       Q9H8W4; P15622-3: ZNF250; NbExp=3; IntAct=EBI-742388, EBI-10177272;
CC       Q9H8W4; Q8TD17: ZNF398; NbExp=3; IntAct=EBI-742388, EBI-8643207;
CC       Q9H8W4; Q8NDP4: ZNF439; NbExp=3; IntAct=EBI-742388, EBI-747580;
CC       Q9H8W4; Q9P0T4: ZNF581; NbExp=3; IntAct=EBI-742388, EBI-745520;
CC   -!- SUBCELLULAR LOCATION: Early endosome membrane
CC       {ECO:0000269|PubMed:19995552, ECO:0000269|PubMed:22816767}; Peripheral
CC       membrane protein {ECO:0000305|PubMed:19995552,
CC       ECO:0000305|PubMed:22816767}. Endoplasmic reticulum
CC       {ECO:0000269|PubMed:18288467}. Note=Colocalizes with EEA1 and RAB5 at
CC       endosomal membrane fusion hot spots (PubMed:19995552). May translocate
CC       to the endoplasmic reticulum in the early phase of apoptosis
CC       (PubMed:18288467). {ECO:0000269|PubMed:18288467,
CC       ECO:0000269|PubMed:19995552}.
CC   -!- TISSUE SPECIFICITY: Expressed in placenta, ovary and small intestine,
CC       as well as in heart and pancreas. Also expressed in peripheral blood
CC       mononuclear cells and dendritic cells. {ECO:0000269|PubMed:18288467}.
CC   -!- INDUCTION: Up-regulated by TNF, bacterial lipopolysaccharides (LPS) and
CC       phorbol myristate acetate (PMA) (at protein level).
CC       {ECO:0000269|PubMed:18288467}.
CC   -!- DOMAIN: The PH and FYVE domains may be important for TNF-induced
CC       localization to the endoplasmic reticulum and for enhanced cellular
CC       sensitivity to TNF-induced apoptosis (PubMed:18288467). The FYVE domain
CC       is important for binding to the endosomal membrane.
CC       {ECO:0000269|PubMed:18288467}.
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DR   EMBL; AF434819; AAL30774.1; -; mRNA.
DR   EMBL; AK023249; BAB14486.1; -; mRNA.
DR   EMBL; AL834473; CAD39132.1; -; mRNA.
DR   EMBL; BC011806; AAH11806.1; -; mRNA.
DR   CCDS; CCDS6267.1; -.
DR   RefSeq; NP_078889.1; NM_024613.3.
DR   BioGRID; 122791; 173.
DR   IntAct; Q9H8W4; 168.
DR   MINT; Q9H8W4; -.
DR   STRING; 9606.ENSP00000322373; -.
DR   iPTMnet; Q9H8W4; -.
DR   PhosphoSitePlus; Q9H8W4; -.
DR   BioMuta; PLEKHF2; -.
DR   DMDM; 74762744; -.
DR   EPD; Q9H8W4; -.
DR   jPOST; Q9H8W4; -.
DR   MassIVE; Q9H8W4; -.
DR   MaxQB; Q9H8W4; -.
DR   PaxDb; Q9H8W4; -.
DR   PeptideAtlas; Q9H8W4; -.
DR   PRIDE; Q9H8W4; -.
DR   ProteomicsDB; 81249; -.
DR   Antibodypedia; 25937; 115 antibodies.
DR   DNASU; 79666; -.
DR   Ensembl; ENST00000315367; ENSP00000322373; ENSG00000175895.
DR   Ensembl; ENST00000519516; ENSP00000427792; ENSG00000175895.
DR   GeneID; 79666; -.
DR   KEGG; hsa:79666; -.
DR   UCSC; uc003yhn.3; human.
DR   CTD; 79666; -.
DR   EuPathDB; HostDB:ENSG00000175895.3; -.
DR   GeneCards; PLEKHF2; -.
DR   HGNC; HGNC:20757; PLEKHF2.
DR   HPA; ENSG00000175895; Low tissue specificity.
DR   MIM; 615208; gene.
DR   neXtProt; NX_Q9H8W4; -.
DR   OpenTargets; ENSG00000175895; -.
DR   PharmGKB; PA128394715; -.
DR   eggNOG; KOG1729; Eukaryota.
DR   GeneTree; ENSGT00940000156408; -.
DR   HOGENOM; CLU_064864_1_0_1; -.
DR   InParanoid; Q9H8W4; -.
DR   KO; K23858; -.
DR   OMA; GEHAAVW; -.
DR   OrthoDB; 1237900at2759; -.
DR   PhylomeDB; Q9H8W4; -.
DR   TreeFam; TF315235; -.
DR   PathwayCommons; Q9H8W4; -.
DR   SIGNOR; Q9H8W4; -.
DR   BioGRID-ORCS; 79666; 10 hits in 868 CRISPR screens.
DR   ChiTaRS; PLEKHF2; human.
DR   GeneWiki; PLEKHF2; -.
DR   GenomeRNAi; 79666; -.
DR   Pharos; Q9H8W4; Tbio.
DR   PRO; PR:Q9H8W4; -.
DR   Proteomes; UP000005640; Chromosome 8.
DR   RNAct; Q9H8W4; protein.
DR   Bgee; ENSG00000175895; Expressed in secondary oocyte and 220 other tissues.
DR   Genevisible; Q9H8W4; HS.
DR   GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0030133; C:transport vesicle; IDA:LIFEdb.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0035091; F:phosphatidylinositol binding; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   CDD; cd01218; PH_Phafin2-like; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   InterPro; IPR037871; PH_Phafin.
DR   InterPro; IPR000306; Znf_FYVE.
DR   InterPro; IPR017455; Znf_FYVE-rel.
DR   InterPro; IPR011011; Znf_FYVE_PHD.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF01363; FYVE; 1.
DR   Pfam; PF00169; PH; 1.
DR   SMART; SM00064; FYVE; 1.
DR   SMART; SM00233; PH; 1.
DR   SUPFAM; SSF57903; SSF57903; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
DR   PROSITE; PS50178; ZF_FYVE; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Endoplasmic reticulum; Endosome; Membrane; Metal-binding;
KW   Phosphoprotein; Protein transport; Reference proteome; Transport; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..249
FT                   /note="Pleckstrin homology domain-containing family F
FT                   member 2"
FT                   /id="PRO_0000251600"
FT   DOMAIN          35..131
FT                   /note="PH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT   ZN_FING         152..212
FT                   /note="FYVE-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00091"
FT   MOD_RES         16
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000244|PubMed:23186163"
FT   MOD_RES         44
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000244|PubMed:19608861"
FT   MOD_RES         239
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000244|PubMed:18669648,
FT                   ECO:0000244|PubMed:20068231, ECO:0000244|PubMed:21406692"
FT   MOD_RES         248
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000244|PubMed:20068231,
FT                   ECO:0000244|PubMed:21406692"
SQ   SEQUENCE   249 AA;  27798 MW;  F5E3F84595A98886 CRC64;
     MVDRLANSEA NTRRISIVEN CFGAAGQPLT IPGRVLIGEG VLTKLCRKKP KARQFFLFND
     ILVYGNIVIQ KKKYNKQHII PLENVTIDSI KDEGDLRNGW LIKTPTKSFA VYAATATEKS
     EWMNHINKCV TDLLSKSGKT PSNEHAAVWV PDSEATVCMR CQKAKFTPVN RRHHCRKCGF
     VVCGPCSEKR FLLPSQSSKP VRICDFCYDL LSAGDMATCQ PARSDSYSQS LKSPLNDMSD
     DDDDDDSSD
//