ID PKRI1_HUMAN Reviewed; 184 AA. AC Q9H875; B4DGM2; Q8NDM6; Q96CF8; DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2001, sequence version 1. DT 25-MAY-2022, entry version 146. DE RecName: Full=PRKR-interacting protein 1; GN Name=PRKRIP1; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). RC TISSUE=Brain, and Thyroid; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., RA Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., RA Hunkapiller M.W., Myers E.W., Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=12853948; DOI=10.1038/nature01782; RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H., RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., RA Wilson R.K.; RT "The DNA sequence of human chromosome 7."; RL Nature 424:157-164(2003). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT TYR-106. RC TISSUE=Lung; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 103-184 (ISOFORM 1). RC TISSUE=Uterus; RX PubMed=17974005; DOI=10.1186/1471-2164-8-399; RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., RA Wiemann S., Schupp I.; RT "The full-ORF clone resource of the German cDNA consortium."; RL BMC Genomics 8:399-399(2007). RN [6] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma; RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026; RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.; RT "Global, in vivo, and site-specific phosphorylation dynamics in signaling RT networks."; RL Cell 127:635-648(2006). RN [7] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21269460; DOI=10.1186/1752-0509-5-17; RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., RA Bennett K.L., Superti-Furga G., Colinge J.; RT "Initial characterization of the human central proteome."; RL BMC Syst. Biol. 5:17-17(2011). RN [8] {ECO:0007744|PDB:5XJC} RP STRUCTURE BY ELECTRON MICROSCOPY (3.60 ANGSTROMS), FUNCTION, SUBCELLULAR RP LOCATION, AND SUBUNIT. RX PubMed=28502770; DOI=10.1016/j.cell.2017.04.033; RA Zhang X., Yan C., Hang J., Finci L.I., Lei J., Shi Y.; RT "An Atomic Structure of the Human Spliceosome."; RL Cell 169:918-929(2017). RN [9] {ECO:0007744|PDB:6QDV} RP STRUCTURE BY ELECTRON MICROSCOPY (3.30 ANGSTROMS) OF 20-139, FUNCTION, RP SUBCELLULAR LOCATION, AND SUBUNIT. RX PubMed=30705154; DOI=10.1126/science.aaw5569; RA Fica S.M., Oubridge C., Wilkinson M.E., Newman A.J., Nagai K.; RT "A human postcatalytic spliceosome structure reveals essential roles of RT metazoan factors for exon ligation."; RL Science 363:710-714(2019). CC -!- FUNCTION: Required for pre-mRNA splicing as component of the CC spliceosome (PubMed:28502770, PubMed:30705154). Binds double-stranded CC RNA. Inhibits EIF2AK2 kinase activity (By similarity). CC {ECO:0000250|UniProtKB:Q9CWV6, ECO:0000269|PubMed:28502770, CC ECO:0000269|PubMed:30705154}. CC -!- SUBUNIT: Component of the pre-catalytic and post-catalytic spliceosome CC complexes (PubMed:28502770, PubMed:30705154). Interacts with EIF2AK2 CC (By similarity). {ECO:0000250|UniProtKB:Q9CWV6, CC ECO:0000269|PubMed:28502770, ECO:0000269|PubMed:30705154}. CC -!- INTERACTION: CC Q9H875; Q8NHQ1: CEP70; NbExp=3; IntAct=EBI-744488, EBI-739624; CC Q9H875; Q12933: TRAF2; NbExp=3; IntAct=EBI-744488, EBI-355744; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28502770, CC ECO:0000269|PubMed:30705154}. Nucleus, nucleolus CC {ECO:0000250|UniProtKB:Q9CWV6}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q9H875-1; Sequence=Displayed; CC Name=2; CC IsoId=Q9H875-2; Sequence=VSP_056088, VSP_056089; CC -!- SIMILARITY: Belongs to the PRKRIP1 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK023964; BAB14742.1; -; mRNA. DR EMBL; AK294664; BAG57833.1; -; mRNA. DR EMBL; AC005088; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AC073517; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AC091390; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471197; EAW50241.1; -; Genomic_DNA. DR EMBL; BC014298; AAH14298.1; -; mRNA. DR EMBL; AL833837; CAD38697.2; -; mRNA. DR CCDS; CCDS34714.1; -. [Q9H875-1] DR RefSeq; NP_078929.1; NM_024653.3. [Q9H875-1] DR PDB; 5XJC; EM; 3.60 A; X=1-184. DR PDB; 6ICZ; EM; 3.00 A; X=1-184. DR PDB; 6QDV; EM; 3.30 A; D=20-139. DR PDBsum; 5XJC; -. DR PDBsum; 6ICZ; -. DR PDBsum; 6QDV; -. DR AlphaFoldDB; Q9H875; -. DR SMR; Q9H875; -. DR BioGRID; 122824; 21. DR CORUM; Q9H875; -. DR IntAct; Q9H875; 14. DR STRING; 9606.ENSP00000419270; -. DR iPTMnet; Q9H875; -. DR PhosphoSitePlus; Q9H875; -. DR BioMuta; PRKRIP1; -. DR DMDM; 74733776; -. DR EPD; Q9H875; -. DR jPOST; Q9H875; -. DR MassIVE; Q9H875; -. DR MaxQB; Q9H875; -. DR PaxDb; Q9H875; -. DR PeptideAtlas; Q9H875; -. DR PRIDE; Q9H875; -. DR ProteomicsDB; 4144; -. DR ProteomicsDB; 81197; -. [Q9H875-1] DR Antibodypedia; 35198; 62 antibodies from 16 providers. DR DNASU; 79706; -. DR Ensembl; ENST00000397912.3; ENSP00000381010.3; ENSG00000128563.13. DR Ensembl; ENST00000496391.5; ENSP00000419270.1; ENSG00000128563.13. DR GeneID; 79706; -. DR KEGG; hsa:79706; -. DR MANE-Select; ENST00000397912.3; ENSP00000381010.3; NM_024653.4; NP_078929.1. DR UCSC; uc003uzh.3; human. [Q9H875-1] DR CTD; 79706; -. DR GeneCards; PRKRIP1; -. DR HGNC; HGNC:21894; PRKRIP1. DR HPA; ENSG00000128563; Low tissue specificity. DR MIM; 617458; gene. DR neXtProt; NX_Q9H875; -. DR OpenTargets; ENSG00000128563; -. DR PharmGKB; PA134952162; -. DR VEuPathDB; HostDB:ENSG00000128563; -. DR eggNOG; KOG4055; Eukaryota. DR GeneTree; ENSGT00390000005003; -. DR HOGENOM; CLU_079129_2_0_1; -. DR InParanoid; Q9H875; -. DR OMA; EPSFIMG; -. DR PhylomeDB; Q9H875; -. DR TreeFam; TF314382; -. DR PathwayCommons; Q9H875; -. DR SignaLink; Q9H875; -. DR BioGRID-ORCS; 79706; 317 hits in 1090 CRISPR screens. DR ChiTaRS; PRKRIP1; human. DR GenomeRNAi; 79706; -. DR Pharos; Q9H875; Tdark. DR PRO; PR:Q9H875; -. DR Proteomes; UP000005640; Chromosome 7. DR RNAct; Q9H875; protein. DR Bgee; ENSG00000128563; Expressed in testis and 214 other tissues. DR ExpressionAtlas; Q9H875; baseline and differential. DR Genevisible; Q9H875; HS. DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB. DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB. DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW. DR GO; GO:0003725; F:double-stranded RNA binding; ISS:UniProtKB. DR GO; GO:0019901; F:protein kinase binding; ISS:UniProtKB. DR GO; GO:0004860; F:protein kinase inhibitor activity; ISS:UniProtKB. DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW. DR GO; GO:0042326; P:negative regulation of phosphorylation; ISS:UniProtKB. DR GO; GO:0006469; P:negative regulation of protein kinase activity; ISS:UniProtKB. DR GO; GO:0003014; P:renal system process; IDA:UniProtKB. DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. DR InterPro; IPR009548; Prkrip1. DR PANTHER; PTHR13507; PTHR13507; 1. DR Pfam; PF06658; DUF1168; 1. PE 1: Evidence at protein level; KW 3D-structure; Alternative splicing; Coiled coil; mRNA processing; KW mRNA splicing; Nucleus; Reference proteome; Spliceosome. FT CHAIN 1..184 FT /note="PRKR-interacting protein 1" FT /id="PRO_0000324787" FT REGION 1..50 FT /note="Interaction with EIF2AK2" FT /evidence="ECO:0000250" FT REGION 1..20 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 37..76 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 51..143 FT /note="Required for RNA-binding" FT /evidence="ECO:0000250" FT REGION 120..184 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 126..138 FT /note="Required for nuclear localization" FT /evidence="ECO:0000250" FT COILED 91..152 FT /evidence="ECO:0000255" FT COMPBIAS 140..154 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT VAR_SEQ 1..38 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000303|PubMed:14702039" FT /id="VSP_056088" FT VAR_SEQ 132..184 FT /note="QKLKEKKLLAKKMKLEQKKQEGPGQPKEQGSSSSAEASGTEEEEEVPSFTMG FT R -> TRSAQGAGVQQLCGGIWNRGGGGSAQFHHGAMTMFATASAWNLARAVTRRERRL FT FGSFSPARTR (in isoform 2)" FT /evidence="ECO:0000303|PubMed:14702039" FT /id="VSP_056089" FT VARIANT 7 FT /note="S -> A (in dbSNP:rs6951185)" FT /id="VAR_039882" FT VARIANT 106 FT /note="D -> Y (in dbSNP:rs11556160)" FT /evidence="ECO:0000269|PubMed:15489334" FT /id="VAR_039883" FT HELIX 78..141 FT /evidence="ECO:0007829|PDB:6ICZ" SQ SEQUENCE 184 AA; 20997 MW; 0374DA48DD8D4B22 CRC64; MASPAASSVR PPRPKKEPQT LVIPKNAAEE QKLKLERLMK NPDKAVPIPE KMSEWAPRPP PEFVRDVMGS SAGAGSGEFH VYRHLRRREY QRQDYMDAMA EKQKLDAEFQ KRLEKNKIAA EEQTAKRRKK RQKLKEKKLL AKKMKLEQKK QEGPGQPKEQ GSSSSAEASG TEEEEEVPSF TMGR //