ID   PPF1_PEA       STANDARD;      PRT;   442 AA.
AC   Q9FY06; O04699;
DT   15-MAR-2004 (Rel. 43, Created)
DT   15-MAR-2004 (Rel. 43, Last sequence update)
DT   15-MAR-2004 (Rel. 43, Last annotation update)
DE   Inner membrane protein PPF-1, chloroplast precursor (Post-floral-
DE   specific protein 1).
GN   PPF-1.
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots; Rosidae;
OC   eurosids I; Fabales; Fabaceae; Papilionoideae; Vicieae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   SEQUENCE FROM N.A.
RC   STRAIN=cv. G2; TISSUE=Apical bud;
RX   MEDLINE=98147997; PubMed=9479033;
RA   Zhu Y., Zhang Y., Luo J., Davies P.J., Ho D.T.-H.;
RT   "PPF-1, a post-floral-specific gene expressed in short-day-grown G2
RT   pea, may be important for its never-senescing phenotype.";
RL   Gene 208:1-6(1998).
RN   [2]
RP   SEQUENCE FROM N.A., AND VARIANTS GLY-107; GLY-127; PRO-150; PRO-250
RP   AND 326-HIS-LYS-327.
RC   STRAIN=cv. Alaska;
RA   Xu Y., Wang M., Li Q., Ji X., Zhu Y.;
RT   "Cloning and sequencing of the homologous gene of PPF-1 from Alaska
RT   pea.";
RL   Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May be required for the insertion of some integral
CC       membrane proteins into the chloroplast thylakoid membrane. May
CC       play a role in inhibiting senescence.
CC   -!- SUBCELLULAR LOCATION: Integral membrane protein. Chloroplast;
CC       thylakoid membrane (Potential).
CC   -!- TISSUE SPECIFICITY: Highly expressed in apical buds. Low levels of
CC       expression in leaves. Not expressed in roots, and stems.
CC   -!- DEVELOPMENTAL STAGE: Expressed after flower initiation.
CC   -!- INDUCTION: By the plant hormone gibberellin A3. Expression is
CC       induced to a greater extent by short day conditions than long day
CC       conditions.
CC   -!- SIMILARITY: Belongs to the OXA1/oxaA family.
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DR   EMBL; Y12618; CAA73179.1; -.
DR   EMBL; AJ297606; CAC04249.1; -.
DR   PIR; T06476; T06476.
DR   InterPro; IPR001708; 60kDa_innermeb.
DR   Pfam; PF02096; 60KD_IMP; 1.
KW   Chloroplast; Membrane; Inner membrane; Transit peptide; Transmembrane.
FT   TRANSIT       1      ?       CHLOROPLAST (POTENTIAL).
FT   CHAIN         ?    442       INNER MEMBRANE PROTEIN PPF-1.
FT   DOMAIN        1    108       LUMENAL (POTENTIAL).
FT   TRANSMEM    109    129       POTENTIAL.
FT   DOMAIN      130    183       STROMAL (POTENTIAL).
FT   TRANSMEM    184    204       POTENTIAL.
FT   DOMAIN      205    296       LUMENAL (POTENTIAL).
FT   TRANSMEM    297    317       POTENTIAL.
FT   DOMAIN      318    442       STROMAL (POTENTIAL).
FT   VARIANT     107    107       D -> G (in strain cv. Alaska).
FT   VARIANT     127    127       V -> G (in strain cv. Alaska).
FT   VARIANT     150    150       L -> P (in strain cv. Alaska).
FT   VARIANT     250    250       L -> P (in strain cv. Alaska).
FT   VARIANT     326    327       QQ -> HK (in strain cv. Alaska).
SQ   SEQUENCE   442 AA;  48238 MW;  3D4D4C99C5BE9B04 CRC64;
     MAKTLISSPS FLGTPLPSLH RTFSPNRTRL FTKVQFSFHQ LPPIQSVSHS VDLSGIFARA
     EGLLYTLADA TVAADAAAST DVAAQKNGGW FGFISDGMEF VLKVLKDGLS SVHVPYSYGF
     AIILLTVIVK AATLPLTKQQ VESTLAMQNL QPKIKAIQER YAGNQERIQL ETSRLYTQAG
     VNPLAGCLPT LATIPVWIGL YQALSNVANE GLLTEGFLWI PSLGGPTSIA ARQSGSGISW
     LFPFVDGHPL LGWYDTAAYL VLPVLLIVSQ YVSMEIMKPP QTNDPNQKNT LLIFKFLPLM
     IGYFSLSVPS GLTIYWFTNN VLSTAQQVWL RKLGGAKPAV NENAGGIITA GQAKRSASKP
     EKGGERFRQL KEEEKKKKLI KALPVEEVQP LASASASNDG SDVENNKEQE VTEESNTSKV
     SQEVQSFSRE RRSKRSKRKP VA
//