ID GLK2_ARATH Reviewed; 386 AA. AC Q9FFH0; Q94A45; DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2001, sequence version 1. DT 12-APR-2017, entry version 119. DE RecName: Full=Transcription activator GLK2; DE AltName: Full=GBF'S PRO-RICH REGION-INTERACTING factor 2; DE AltName: Full=Golden2-like protein 2; DE Short=AtGLK2; GN Name=GLK2; Synonyms=GPRI2; OrderedLocusNames=At5g44190; GN ORFNames=MLN1.11; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; OC Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION. RX PubMed=11828027; DOI=10.1093/pcp/pcf011; RA Tamai H., Iwabuchi M., Meshi T.; RT "Arabidopsis GARP transcriptional activators interact with the Pro- RT rich activation domain shared by G-box-binding bZIP factors."; RL Plant Cell Physiol. 43:99-107(2002). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE RP SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE. RX PubMed=12220263; DOI=10.1046/j.1365-313X.2002.01390.x; RA Fitter D.W., Martin D.J., Copley M.J., Scotland R.W., Langdale J.A.; RT "GLK gene pairs regulate chloroplast development in diverse plant RT species."; RL Plant J. 31:713-727(2002). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=9330910; DOI=10.1093/dnares/4.3.215; RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., RA Miyajima N., Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence RT features of the 1.6 Mb regions covered by twenty physically assigned RT P1 clones."; RL DNA Res. 4:215-230(1997). RN [4] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Portal (Araport); RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [6] RP FUNCTION. RX PubMed=18643989; DOI=10.1111/j.1365-313X.2008.03616.x; RA Waters M.T., Moylan E.C., Langdale J.A.; RT "GLK transcription factors regulate chloroplast development in a cell- RT autonomous manner."; RL Plant J. 56:432-444(2008). RN [7] RP FUNCTION. RX PubMed=19383092; DOI=10.1111/j.1469-8137.2009.02829.x; RA Bravo-Garcia A., Yasumura Y., Langdale J.A.; RT "Specialization of the Golden2-like regulatory pathway during land RT plant evolution."; RL New Phytol. 183:133-141(2009). RN [8] RP FUNCTION. RX PubMed=19376934; DOI=10.1105/tpc.108.065250; RA Waters M.T., Wang P., Korkaric M., Capper R.G., Saunders N.J., RA Langdale J.A.; RT "GLK transcription factors coordinate expression of the photosynthetic RT apparatus in Arabidopsis."; RL Plant Cell 21:1109-1128(2009). RN [9] RP FUNCTION. RX PubMed=19726569; DOI=10.1104/pp.109.145987; RA Kakizaki T., Matsumura H., Nakayama K., Che F.S., Terauchi R., RA Inaba T.; RT "Coordination of plastid protein import and nuclear gene expression by RT plastid-to-nucleus retrograde signaling."; RL Plant Physiol. 151:1339-1353(2009). RN [10] RP INDUCTION. RX PubMed=21214652; DOI=10.1111/j.1365-313X.2010.04449.x; RA Yu X., Li L., Zola J., Aluru M., Ye H., Foudree A., Guo H., RA Anderson S., Aluru S., Liu P., Rodermel S., Yin Y.; RT "A brassinosteroid transcriptional network revealed by genome-wide RT identification of BESI target genes in Arabidopsis thaliana."; RL Plant J. 65:634-646(2011). RN [11] RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH NAC92, AND RP SUBCELLULAR LOCATION. RC STRAIN=cv. Columbia; RX PubMed=23459204; DOI=10.1038/embor.2013.24; RA Rauf M., Arif M., Dortay H., Matallana-Ramirez L.P., Waters M.T., RA Gil Nam H., Lim P.-O., Mueller-Roeber B., Balazadeh S.; RT "ORE1 balances leaf senescence against maintenance by antagonizing G2- RT like-mediated transcription."; RL EMBO Rep. 14:382-388(2013). CC -!- FUNCTION: Transcriptional activator that functions with GLK1 to CC promote chloroplast development. Acts as an activator of nuclear CC photosynthetic genes involved in chlorophyll biosynthesis, light CC harvesting, and electron transport. Acts in a cell-autonomous CC manner to coordinate and maintain the photosynthetic apparatus CC within individual cells. May function in photosynthetic capacity CC optimization by integrating responses to variable environmental CC and endogenous cues (PubMed:11828027, PubMed:12220263, CC PubMed:18643989, PubMed:19376934, PubMed:19383092, CC PubMed:19726569). Prevents premature senescence (PubMed:23459204). CC {ECO:0000269|PubMed:11828027, ECO:0000269|PubMed:12220263, CC ECO:0000269|PubMed:18643989, ECO:0000269|PubMed:19376934, CC ECO:0000269|PubMed:19383092, ECO:0000269|PubMed:19726569, CC ECO:0000269|PubMed:23459204}. CC -!- SUBUNIT: Interacts with NAC92. {ECO:0000269|PubMed:23459204}. CC -!- INTERACTION: CC Q9FKA0:NAC92; NbExp=6; IntAct=EBI-6862475, EBI-6862413; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23459204}. CC -!- TISSUE SPECIFICITY: Expressed in cotyledons and rosette and CC cauline leaves. Expressed at low levels in roots, shoots, flowers CC and siliques. {ECO:0000269|PubMed:12220263}. CC -!- INDUCTION: By light. Repressed by BZR2. CC {ECO:0000269|PubMed:12220263, ECO:0000269|PubMed:21214652}. CC -!- DISRUPTION PHENOTYPE: Pale green siliques (PubMed:12220263). Pale- CC green seedlings in double mutants glk1/glk2 (PubMed:23459204). CC {ECO:0000269|PubMed:12220263, ECO:0000269|PubMed:23459204}. CC -!- MISCELLANEOUS: Plants overexpressing GLK2 have a delay in CC flowering under long days. CC -!- SEQUENCE CAUTION: CC Sequence=AAK91403.1; Type=Erroneous termination; Positions=315; Note=Translated as Ser.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB062490; BAB78467.1; -; mRNA. DR EMBL; AY026773; AAK16744.1; -; mRNA. DR EMBL; AY028368; AAK20121.1; -; Genomic_DNA. DR EMBL; AB005239; BAB10986.1; -; Genomic_DNA. DR EMBL; CP002688; AED95072.1; -; Genomic_DNA. DR EMBL; AY050386; AAK91403.1; ALT_SEQ; mRNA. DR EMBL; AY124819; AAM70528.1; -; mRNA. DR RefSeq; NP_199232.1; NM_123786.4. DR UniGene; At.19638; -. DR ProteinModelPortal; Q9FFH0; -. DR SMR; Q9FFH0; -. DR BioGrid; 19692; 2. DR IntAct; Q9FFH0; 1. DR STRING; 3702.AT5G44190.1; -. DR PaxDb; Q9FFH0; -. DR EnsemblPlants; AT5G44190.1; AT5G44190.1; AT5G44190. DR GeneID; 834442; -. DR Gramene; AT5G44190.1; AT5G44190.1; AT5G44190. DR KEGG; ath:AT5G44190; -. DR Araport; AT5G44190; -. DR TAIR; locus:2167639; AT5G44190. DR eggNOG; ENOG410IH7K; Eukaryota. DR eggNOG; ENOG4111SK7; LUCA. DR HOGENOM; HOG000237698; -. DR InParanoid; Q9FFH0; -. DR OMA; KPKVDWT; -. DR OrthoDB; EOG09360HCC; -. DR PhylomeDB; Q9FFH0; -. DR PRO; PR:Q9FFH0; -. DR Proteomes; UP000006548; Chromosome 5. DR Genevisible; Q9FFH0; AT. DR GO; GO:0005634; C:nucleus; IDA:UniProtKB. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0003700; F:transcription factor activity, sequence-specific DNA binding; ISS:TAIR. DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR. DR GO; GO:0009910; P:negative regulation of flower development; IGI:TAIR. DR GO; GO:1900056; P:negative regulation of leaf senescence; IMP:UniProtKB. DR GO; GO:0010638; P:positive regulation of organelle organization; IEA:EnsemblPlants. DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR. DR GO; GO:0010380; P:regulation of chlorophyll biosynthetic process; IGI:TAIR. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW. DR Gene3D; 1.10.10.60; -; 1. DR InterPro; IPR009057; Homeobox-like. DR InterPro; IPR017930; Myb_dom. DR InterPro; IPR006447; Myb_dom_plants. DR InterPro; IPR001005; SANT/Myb. DR Pfam; PF00249; Myb_DNA-binding; 1. DR SUPFAM; SSF46689; SSF46689; 1. DR TIGRFAMs; TIGR01557; myb_SHAQKYF; 1. DR PROSITE; PS51294; HTH_MYB; 1. PE 1: Evidence at protein level; KW Complete proteome; DNA-binding; Nucleus; Reference proteome; KW Transcription; Transcription regulation. FT CHAIN 1 386 Transcription activator GLK2. FT /FTId=PRO_0000408386. FT DNA_BIND 144 203 Myb-like GARP. {ECO:0000255|PROSITE- FT ProRule:PRU00625}. SQ SEQUENCE 386 AA; 42167 MW; A61AFE189A98C606 CRC64; MLTVSPAPVL IGNNSKDTYM AADFADFTTE DLPDFTTVGD FSDDLLDGID YYDDLFIGFD GDDVLPDLEI DSEILGEYSG SGRDEEQEME GNTSTASETS ERDVGVCKQE GGGGGDGGFR DKTVRRGKRK GKKSKDCLSD ENDIKKKPKV DWTPELHRKF VQAVEQLGVD KAVPSRILEI MNVKSLTRHN VASHLQKYRS HRKHLLAREA EAASWNLRRH ATVAVPGVGG GGKKPWTAPA LGYPPHVAPM HHGHFRPLHV WGHPTWPKHK PNTPASAHRT YPMPAIAAAP ASWPGHPPYW HQQPLYPQGY GMASSNHSSI GVPTRQLGPT NPPIDIHPSN ESIDAAIGDV ISKPWLPLPL GLKPPSVDGV MTELQRQGVS NVPPLP //