ID   MICU3_MOUSE             Reviewed;         523 AA.
AC   Q9CTY5;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 2.
DT   24-JAN-2024, entry version 137.
DE   RecName: Full=Calcium uptake protein 3, mitochondrial;
DE   AltName: Full=EF-hand domain-containing family member A2;
GN   Name=Micu3; Synonyms=Efha2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 140-258.
RC   STRAIN=C57BL/6J; TISSUE=Hippocampus;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   TISSUE SPECIFICITY.
RX   PubMed=23409044; DOI=10.1371/journal.pone.0055785;
RA   Plovanich M., Bogorad R.L., Sancak Y., Kamer K.J., Strittmatter L.,
RA   Li A.A., Girgis H.S., Kuchimanchi S., De Groot J., Speciner L., Taneja N.,
RA   Oshea J., Koteliansky V., Mootha V.K.;
RT   "MICU2, a paralog of MICU1, resides within the mitochondrial uniporter
RT   complex to regulate calcium handling.";
RL   PLoS ONE 8:E55785-E55785(2013).
CC   -!- FUNCTION: May play a role in mitochondrial calcium uptake.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}. Membrane
CC       {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in skeletal muscle and
CC       central nervous system. {ECO:0000269|PubMed:23409044}.
CC   -!- MISCELLANEOUS: Binds calcium. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MICU1 family. MICU3 subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB31674.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AC102680; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC160537; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK019353; BAB31674.1; ALT_INIT; mRNA.
DR   CCDS; CCDS52545.1; -.
DR   RefSeq; NP_084386.1; NM_030110.1.
DR   AlphaFoldDB; Q9CTY5; -.
DR   SMR; Q9CTY5; -.
DR   BioGRID; 219445; 5.
DR   IntAct; Q9CTY5; 1.
DR   MINT; Q9CTY5; -.
DR   STRING; 10090.ENSMUSP00000070241; -.
DR   GlyGen; Q9CTY5; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q9CTY5; -.
DR   PhosphoSitePlus; Q9CTY5; -.
DR   SwissPalm; Q9CTY5; -.
DR   MaxQB; Q9CTY5; -.
DR   PaxDb; 10090-ENSMUSP00000070241; -.
DR   PeptideAtlas; Q9CTY5; -.
DR   ProteomicsDB; 252563; -.
DR   Antibodypedia; 8886; 75 antibodies from 17 providers.
DR   Ensembl; ENSMUST00000068999.14; ENSMUSP00000070241.8; ENSMUSG00000039478.16.
DR   GeneID; 78506; -.
DR   KEGG; mmu:78506; -.
DR   UCSC; uc009lmm.2; mouse.
DR   AGR; MGI:1925756; -.
DR   CTD; 286097; -.
DR   MGI; MGI:1925756; Micu3.
DR   VEuPathDB; HostDB:ENSMUSG00000039478; -.
DR   eggNOG; KOG2643; Eukaryota.
DR   GeneTree; ENSGT00950000183079; -.
DR   HOGENOM; CLU_027103_0_2_1; -.
DR   InParanoid; Q9CTY5; -.
DR   OMA; VHFFGPK; -.
DR   OrthoDB; 1020411at2759; -.
DR   PhylomeDB; Q9CTY5; -.
DR   TreeFam; TF320374; -.
DR   Reactome; R-MMU-8949215; Mitochondrial calcium ion transport.
DR   Reactome; R-MMU-8949664; Processing of SMDT1.
DR   BioGRID-ORCS; 78506; 4 hits in 79 CRISPR screens.
DR   PRO; PR:Q9CTY5; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; Q9CTY5; Protein.
DR   Bgee; ENSMUSG00000039478; Expressed in ventromedial nucleus of hypothalamus and 222 other cell types or tissues.
DR   ExpressionAtlas; Q9CTY5; baseline and differential.
DR   GO; GO:1990246; C:uniplex complex; IBA:GO_Central.
DR   GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR   GO; GO:0036444; P:calcium import into the mitochondrion; IBA:GO_Central.
DR   GO; GO:0051560; P:mitochondrial calcium ion homeostasis; IBA:GO_Central.
DR   CDD; cd16175; EFh_MICU3; 1.
DR   Gene3D; 1.10.238.10; EF-hand; 2.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   InterPro; IPR039800; MICU1/2/3.
DR   PANTHER; PTHR12294:SF10; CALCIUM UPTAKE PROTEIN 3, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR12294; EF HAND DOMAIN FAMILY A1,A2-RELATED; 1.
DR   Pfam; PF13499; EF-hand_7; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; EF-hand; 2.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 3.
PE   2: Evidence at transcript level;
KW   Calcium; Membrane; Metal-binding; Mitochondrion; Reference proteome;
KW   Repeat; Transmembrane; Transmembrane helix.
FT   CHAIN           1..523
FT                   /note="Calcium uptake protein 3, mitochondrial"
FT                   /id="PRO_0000251231"
FT   TRANSMEM        66..82
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          226..261
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          395..430
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          464..499
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         238
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         240
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         242
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         244
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         249
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305"
FT   BINDING         476
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         478
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         480
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         482
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         487
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ   SEQUENCE   523 AA;  59810 MW;  FD8A996423E6A146 CRC64;
     MAALRRFLWP PPRLSPALAP QQPFLSPWGR PAGTAPGMSG RPFSCREEDE GAVAEAAWRR
     RRWGELSIAA AAGGGLVGLV CYQLYGDPRA DPSELAAPEL EDPPRGRGLL PIPVAAAKET
     VATGRAITED LDLYATSRER RFRLFASIEC EGQLFMTPYD FILAVTTDEP KFAKTWKSLS
     KQELSQMLSE TPPVWKGSSK LFRNLKERGV ISYTEYLFLL CILTKPHAGF RIAFNMFDTD
     GNEMVDKKEF LVLQEIFRKK NEKRETKGDE EKRAMLRLQL YGYHSPTNSV LKTDAGELVS
     RSYWDTLRRS TSQALFSDLA ERADDITSLV ADTTLLVHFF GKKGKAELNF EDFYRFMDNL
     QTEVLEIEFL SYSNGMNTIS EEDFAHILLR YTNVENTSVF LENVRYSISE EKGITFDEFR
     SFFQFLNNLE DFAIALNMYN FASRSIGQDE FKRAVYVATG LKLSPHLVNT VFKIFDVDKD
     DQLSYKEFIG IMKDRLHRGF RGYKTVQKYP TFKSCLKKEL HSR
//