ID TRYD_HUMAN Reviewed; 235 AA. AC Q9BZJ3; O95824; Q8TDI6; Q96L36; Q96RZ5; Q9H2Y6; Q9UQI8; DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-2001, sequence version 1. DT 10-JUN-2008, entry version 61. DE Tryptase delta precursor (EC 3.4.21.59) (Delta-tryptase) (Mast cell DE mMCP-7-like) (Tryptase-3) (HmMCP-3-like tryptase III). GN Name=TPSD1; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ARG-15 AND MET-76. RX MEDLINE=99121069; PubMed=9920877; DOI=10.1074/jbc.274.6.3355; RA Pallaoro M., Fejzo M.S., Shayesteh L., Blount J.L., Caughey G.H.; RT "Characterization of genes encoding known and novel human mast cell RT tryptases on chromosome 16p13.3."; RL J. Biol. Chem. 274:3355-3362(1999). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=21101554; PubMed=11174199; DOI=10.1067/mai.2001.112130; RA Min H.-K., Kambe N., Schwartz L.B.; RT "Human mouse mast cell protease 7-like tryptase genes are RT pseudogenes."; RL J. Allergy Clin. Immunol. 107:315-321(2001). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX MEDLINE=21096910; PubMed=11157797; DOI=10.1093/hmg/10.4.339; RA Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., RA Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., RA Higgs D.R.; RT "Sequence, structure and pathology of the fully annotated terminal 2 RT Mb of the short arm of human chromosome 16."; RL Hum. Mol. Genet. 10:339-352(2001). RN [4] RP NUCLEOTIDE SEQUENCE [MRNA] OF 33-235 (ISOFORMS 1 AND 2), ENZYME RP ACTIVITY, TISSUE SPECIFICITY, AND VARIANT MET-76. RX MEDLINE=22278650; PubMed=12391231; RA Wang H.-W., McNeil H.P., Husain A., Liu K., Tedla N., Thomas P.S., RA Raftery M., King G.C., Cai Z.Y., Hunt J.E.; RT "Delta tryptase is expressed in multiple human tissues, and a RT recombinant form has proteolytic activity."; RL J. Immunol. 169:5145-5152(2002). CC -!- FUNCTION: Tryptase is the major neutral protease present in mast CC cells and is secreted upon the coupled activation-degranulation CC response of this cell type (By similarity). CC -!- CATALYTIC ACTIVITY: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, CC but with more restricted specificity than trypsin. CC -!- SUBUNIT: Homotetramer (By similarity). CC -!- SUBCELLULAR LOCATION: Secreted (By similarity). Note=Released from CC the secretory granules upon mast cell activation (By similarity). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q9BZJ3-1; Sequence=Displayed; CC Name=2; CC IsoId=Q9BZJ3-2; Sequence=VSP_008319; CC -!- TISSUE SPECIFICITY: Expressed in colon, lung, heart and synovial CC tissue. May be specific to mast cells. CC -!- SIMILARITY: Belongs to the peptidase S1 family. Tryptase CC subfamily. CC -!- SIMILARITY: Contains 1 peptidase S1 domain. CC -!- CAUTION: Although Ref.2 reported this as a pseudogene, Ref.4 CC showed it is expressed and has proteolytic activity when expressed CC in bacterial cells. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF098327; AAD17845.1; ALT_INIT; Genomic_DNA. DR EMBL; AF099147; AAD17861.1; -; Genomic_DNA. DR EMBL; AF318074; AAK12909.1; -; Genomic_DNA. DR EMBL; AE006466; AAK61272.1; ALT_INIT; Genomic_DNA. DR EMBL; AF206664; AAG35694.1; -; mRNA. DR EMBL; AF421357; AAL86695.1; -; mRNA. DR EMBL; AY055427; AAL17874.1; -; mRNA. DR UniGene; Hs.677789; -. DR HSSP; P20231; 1A0L. DR SMR; Q9BZJ3; 31-235. DR MEROPS; S01.054; -. DR PhosphoSite; Q9BZJ3; -. DR Ensembl; ENSG00000095917; Homo sapiens. DR H-InvDB; HIX0029969; -. DR HGNC; HGNC:14118; TPSD1. DR HPA; CAB002215; -. DR MIM; 609272; gene. DR PharmGKB; PA37845; -. DR HOVERGEN; Q9BZJ3; -. DR ArrayExpress; Q9BZJ3; -. DR CleanEx; HS_TPSD1; -. DR GermOnline; ENSG00000095917; Homo sapiens. DR GO; GO:0008236; F:serine-type peptidase activity; TAS:ProtInc. DR InterPro; IPR001254; Peptidase_S1_S6. DR InterPro; IPR001314; Peptidase_S1A. DR Pfam; PF00089; Trypsin; 1. DR PRINTS; PR00722; CHYMOTRYPSIN. DR SMART; SM00020; Tryp_SPc; 1. DR PROSITE; PS50240; TRYPSIN_DOM; 1. DR PROSITE; PS00134; TRYPSIN_HIS; 1. DR PROSITE; PS00135; TRYPSIN_SER; 1. PE 1: Evidence at protein level; KW Alternative splicing; Glycoprotein; Hydrolase; Polymorphism; Protease; KW Secreted; Serine protease; Signal; Zymogen. FT SIGNAL 1 18 Potential. FT PROPEP 19 30 Activation peptide (By similarity). FT /FTId=PRO_0000027483. FT CHAIN 31 235 Tryptase delta. FT /FTId=PRO_0000027484. FT DOMAIN 31 235 Peptidase S1. FT ACT_SITE 74 74 Charge relay system (By similarity). FT ACT_SITE 121 121 Charge relay system (By similarity). FT ACT_SITE 224 224 Charge relay system (By similarity). FT CARBOHYD 132 132 N-linked (GlcNAc...) (Potential). FT DISULFID 59 75 By similarity. FT DISULFID 155 230 By similarity. FT DISULFID 188 211 By similarity. FT VAR_SEQ 79 87 Missing (in isoform 2). FT /FTId=VSP_008319. FT VARIANT 15 15 P -> R (in dbSNP:rs3865205). FT /FTId=VAR_016870. FT VARIANT 18 18 V -> A (in dbSNP:rs1800984). FT /FTId=VAR_016871. FT VARIANT 76 76 V -> M (in dbSNP:rs3993987). FT /FTId=VAR_016872. FT CONFLICT 18 18 V -> G (in Ref. 1; AAD17861). FT CONFLICT 171 171 P -> S (in Ref. 4; AAL17874). SQ SEQUENCE 235 AA; 25817 MW; B1FFB6C2A8006B22 CRC64; MLSLLLLALP VLASPAYVAP APGQALQQTG IVGGQEAPRS KWPWQVSLRV RGPYWMHFCG GSLIHPQWVL TAAHCVEPDI KDLAALRVQL REQHLYYQDQ LLPVSRIIVH PQFYIIQTGA DIALLELEEP VNISSHIHTV TLPPASETFP PGMPCWVTGW GDVDNNVHLP PPYPLKEVEV PVVENHLCNA EYHTGLHTGH SFQIVRDDML CAGSENHDSC QGDSGGPLVC KVNGT //