ID   LDH6B_HUMAN             Reviewed;         381 AA.
AC   Q9BYZ2; Q6DUY4; Q96LI2;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 3.
DT   05-FEB-2025, entry version 185.
DE   RecName: Full=L-lactate dehydrogenase A-like 6B;
DE            EC=1.1.1.27;
GN   Name=LDHAL6B; Synonyms=LDHAL6, LDHL;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RA   Sha J.H., Li J.M., Zhou Z.M.;
RT   "A novel intronless human testis lactate dehydrogenase gene from adult
RT   testis.";
RL   Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Tang W.W.;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS MET-14; LEU-30 AND
RP   THR-326.
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS MET-14; LEU-30 AND
RP   THR-326.
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-lactate + NAD(+) = pyruvate + NADH + H(+);
CC         Xref=Rhea:RHEA:23444, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16651, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.27;
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-lactate
CC       from pyruvate: step 1/1.
CC   -!- INTERACTION:
CC       Q9BYZ2; P02649: APOE; NbExp=3; IntAct=EBI-1108377, EBI-1222467;
CC       Q9BYZ2; P05067: APP; NbExp=3; IntAct=EBI-1108377, EBI-77613;
CC       Q9BYZ2; P05067-2: APP; NbExp=3; IntAct=EBI-1108377, EBI-17264467;
CC       Q9BYZ2; P54253: ATXN1; NbExp=6; IntAct=EBI-1108377, EBI-930964;
CC       Q9BYZ2; P54252: ATXN3; NbExp=3; IntAct=EBI-1108377, EBI-946046;
CC       Q9BYZ2; Q14203-5: DCTN1; NbExp=3; IntAct=EBI-1108377, EBI-25840379;
CC       Q9BYZ2; Q14103: HNRNPD; NbExp=2; IntAct=EBI-1108377, EBI-299674;
CC       Q9BYZ2; P42858: HTT; NbExp=18; IntAct=EBI-1108377, EBI-466029;
CC       Q9BYZ2; Q92597: NDRG1; NbExp=3; IntAct=EBI-1108377, EBI-716486;
CC       Q9BYZ2; Q96CV9: OPTN; NbExp=3; IntAct=EBI-1108377, EBI-748974;
CC       Q9BYZ2; Q99497: PARK7; NbExp=3; IntAct=EBI-1108377, EBI-1164361;
CC       Q9BYZ2; Q9BXM7: PINK1; NbExp=3; IntAct=EBI-1108377, EBI-2846068;
CC       Q9BYZ2; A0A6Q8PF08: PMP22; NbExp=3; IntAct=EBI-1108377, EBI-50433196;
CC       Q9BYZ2; O60260-5: PRKN; NbExp=6; IntAct=EBI-1108377, EBI-21251460;
CC       Q9BYZ2; P49768-2: PSEN1; NbExp=6; IntAct=EBI-1108377, EBI-11047108;
CC       Q9BYZ2; P37840: SNCA; NbExp=3; IntAct=EBI-1108377, EBI-985879;
CC       Q9BYZ2; P00441: SOD1; NbExp=3; IntAct=EBI-1108377, EBI-990792;
CC       Q9BYZ2; Q13148: TARDBP; NbExp=6; IntAct=EBI-1108377, EBI-372899;
CC       Q9BYZ2; Q9NUW8: TDP1; NbExp=3; IntAct=EBI-1108377, EBI-2902553;
CC       Q9BYZ2; P09936: UCHL1; NbExp=3; IntAct=EBI-1108377, EBI-714860;
CC   -!- TISSUE SPECIFICITY: Testis specific.
CC   -!- DEVELOPMENTAL STAGE: Higher expression level in adult testis as
CC       compared to 6-week-old fetal testis.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
CC       {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-50 is the initiator.
CC       {ECO:0000305}.
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DR   EMBL; AY009108; AAG49399.1; -; mRNA.
DR   EMBL; AY642121; AAT65080.1; -; mRNA.
DR   EMBL; AK058192; BAB71710.1; -; mRNA.
DR   EMBL; BC022034; AAH22034.1; -; mRNA.
DR   CCDS; CCDS10171.1; -.
DR   RefSeq; NP_149972.1; NM_033195.2.
DR   AlphaFoldDB; Q9BYZ2; -.
DR   SMR; Q9BYZ2; -.
DR   BioGRID; 124950; 51.
DR   ComplexPortal; CPX-6622; L-lactate dehydrogenase complex, LDHAL6B variant.
DR   IntAct; Q9BYZ2; 37.
DR   MINT; Q9BYZ2; -.
DR   STRING; 9606.ENSP00000302393; -.
DR   DrugBank; DB00157; NADH.
DR   GlyGen; Q9BYZ2; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q9BYZ2; -.
DR   PhosphoSitePlus; Q9BYZ2; -.
DR   BioMuta; LDHAL6B; -.
DR   DMDM; 116242616; -.
DR   jPOST; Q9BYZ2; -.
DR   MassIVE; Q9BYZ2; -.
DR   PaxDb; 9606-ENSP00000302393; -.
DR   PeptideAtlas; Q9BYZ2; -.
DR   ProteomicsDB; 79743; -.
DR   Antibodypedia; 25391; 172 antibodies from 24 providers.
DR   DNASU; 92483; -.
DR   Ensembl; ENST00000307144.6; ENSP00000302393.4; ENSG00000171989.6.
DR   GeneID; 92483; -.
DR   KEGG; hsa:92483; -.
DR   MANE-Select; ENST00000307144.6; ENSP00000302393.4; NM_033195.3; NP_149972.1.
DR   UCSC; uc002agb.5; human.
DR   AGR; HGNC:21481; -.
DR   CTD; 92483; -.
DR   GeneCards; LDHAL6B; -.
DR   HGNC; HGNC:21481; LDHAL6B.
DR   HPA; ENSG00000171989; Tissue enriched (testis).
DR   neXtProt; NX_Q9BYZ2; -.
DR   OpenTargets; ENSG00000171989; -.
DR   PharmGKB; PA134943157; -.
DR   VEuPathDB; HostDB:ENSG00000171989; -.
DR   eggNOG; KOG1495; Eukaryota.
DR   GeneTree; ENSGT00940000163242; -.
DR   HOGENOM; CLU_045401_0_2_1; -.
DR   InParanoid; Q9BYZ2; -.
DR   OMA; RVIGNGC; -.
DR   OrthoDB; 5405561at2759; -.
DR   PhylomeDB; Q9BYZ2; -.
DR   TreeFam; TF314963; -.
DR   PathwayCommons; Q9BYZ2; -.
DR   Reactome; R-HSA-70268; Pyruvate metabolism.
DR   SignaLink; Q9BYZ2; -.
DR   UniPathway; UPA00554; UER00611.
DR   BioGRID-ORCS; 92483; 14 hits in 1140 CRISPR screens.
DR   GenomeRNAi; 92483; -.
DR   Pharos; Q9BYZ2; Tbio.
DR   PRO; PR:Q9BYZ2; -.
DR   Proteomes; UP000005640; Chromosome 15.
DR   RNAct; Q9BYZ2; protein.
DR   Bgee; ENSG00000171989; Expressed in right testis and 71 other cell types or tissues.
DR   ExpressionAtlas; Q9BYZ2; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IDA:ComplexPortal.
DR   GO; GO:0005759; C:mitochondrial matrix; TAS:Reactome.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR   GO; GO:1990204; C:oxidoreductase complex; NAS:ComplexPortal.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0006089; P:lactate metabolic process; IDA:ComplexPortal.
DR   GO; GO:0006090; P:pyruvate metabolic process; IDA:ComplexPortal.
DR   CDD; cd05293; LDH_1; 1.
DR   FunFam; 3.40.50.720:FF:000029; L-lactate dehydrogenase A chain; 1.
DR   FunFam; 3.90.110.10:FF:000003; L-lactate dehydrogenase A chain; 1.
DR   Gene3D; 3.90.110.10; Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   HAMAP; MF_00488; Lactate_dehydrog; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR011304; L-lactate_DH.
DR   InterPro; IPR018177; L-lactate_DH_AS.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   NCBIfam; TIGR01771; L-LDH-NAD; 1.
DR   PANTHER; PTHR43128; L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+)); 1.
DR   PANTHER; PTHR43128:SF8; L-LACTATE DEHYDROGENASE A-LIKE 6B; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   1: Evidence at protein level;
KW   NAD; Oxidoreductase; Proteomics identification; Reference proteome.
FT   CHAIN           1..381
FT                   /note="L-lactate dehydrogenase A-like 6B"
FT                   /id="PRO_0000168458"
FT   ACT_SITE        242
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         101..106
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         148
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         155
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         187
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         187
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         218
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         297
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   VARIANT         14
FT                   /note="V -> M (in dbSNP:rs3809530)"
FT                   /evidence="ECO:0000269|PubMed:14702039,
FT                   ECO:0000269|PubMed:15489334"
FT                   /id="VAR_027936"
FT   VARIANT         30
FT                   /note="P -> L (in dbSNP:rs3809529)"
FT                   /evidence="ECO:0000269|PubMed:14702039,
FT                   ECO:0000269|PubMed:15489334"
FT                   /id="VAR_027937"
FT   VARIANT         259
FT                   /note="P -> S (in dbSNP:rs35212259)"
FT                   /id="VAR_049757"
FT   VARIANT         326
FT                   /note="I -> T (in dbSNP:rs3825937)"
FT                   /evidence="ECO:0000269|PubMed:14702039,
FT                   ECO:0000269|PubMed:15489334"
FT                   /id="VAR_027938"
SQ   SEQUENCE   381 AA;  41943 MW;  F2B2D27E3ADE8A1D CRC64;
     MSWTVPVVRA SQRVSSVGAN FLCLGMALCP RQATRIPLNG TWLFTPVSKM ATVKSELIER
     FTSEKPVHHS KVSIIGTGSV GMACAISILL KGLSDELALV DLDEDKLKGE TMDLQHGSPF
     TKMPNIVCSK DYFVTANSNL VIITAGARQE KGETRLNLVQ RNVAIFKLMI SSIVQYSPHC
     KLIIVSNPVD ILTYVAWKLS AFPKNRIIGS GCNLDTARFR FLIGQKLGIH SESCHGWILG
     EHGDSSVPVW SGVNIAGVPL KDLNSDIGTD KDPEQWKNVH KEVTATAYEI IKMKGYTSWA
     IGLSVADLTE SILKNLRRIH PVSTIIKGLY GIDEEVFLSI PCILGENGIT NLIKIKLTPE
     EEAHLKKSAK TLWEIQNKLK L
//