ID ABM3_MOUSE STANDARD; PRT; 429 AA. AC Q99J72; Q8C7L0; Q8C8V7; DT 05-JUL-2004 (Rel. 44, Created) DT 05-JUL-2004 (Rel. 44, Last sequence update) DT 05-JUL-2004 (Rel. 44, Last annotation update) DE DNA dC->dU editing enzyme APOBEC-3 (EC 3.5.4.-) (Apolipoprotein B DE editing complex 3) (Arp3) (CEM15) (CEM-15). GN Name=Apobec3; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. OX NCBI_TaxID=10090; RN [1] RP SEQUENCE FROM N.A. (ISOFORMS 2 AND 3), AND VARIANTS ARG-34; RP 37-ILE-ASP-38; 112-VAL-LEU-113; 134-ILE-ARG-135; ASN-139; LYS-181; RP 233-VAL-HIS-LEU-235; GLY-258; ILE-306 AND HIS-404. RC STRAIN=C57BL/6J; TISSUE=Hippocampus, and Retina; RX MEDLINE=22354683; PubMed=12466851; DOI=10.1038/nature01266; RA Okazaki Y., Furuno M., Kasukawa T., Adachi J., Bono H., Kondo S., RA Nikaido I., Osato N., Saito R., Suzuki H., Yamanaka I., Kiyosawa H., RA Yagi K., Tomaru Y., Hasegawa Y., Nogami A., Schonbach C., Gojobori T., RA Baldarelli R., Hill D.P., Bult C., Hume D.A., Quackenbush J., RA Schriml L.M., Kanapin A., Matsuda H., Batalov S., Beisel K.W., RA Blake J.A., Bradt D., Brusic V., Chothia C., Corbani L.E., Cousins S., RA Dalla E., Dragani T.A., Fletcher C.F., Forrest A., Frazer K.S., RA Gaasterland T., Gariboldi M., Gissi C., Godzik A., Gough J., RA Grimmond S., Gustincich S., Hirokawa N., Jackson I.J., Jarvis E.D., RA Kanai A., Kawaji H., Kawasawa Y., Kedzierski R.M., King B.L., RA Konagaya A., Kurochkin I.V., Lee Y., Lenhard B., Lyons P.A., RA Maglott D.R., Maltais L., Marchionni L., McKenzie L., Miki H., RA Nagashima T., Numata K., Okido T., Pavan W.J., Pertea G., Pesole G., RA Petrovsky N., Pillai R., Pontius J.U., Qi D., Ramachandran S., RA Ravasi T., Reed J.C., Reed D.J., Reid J., Ring B.Z., Ringwald M., RA Sandelin A., Schneider C., Semple C.A., Setou M., Shimada K., RA Sultana R., Takenaka Y., Taylor M.S., Teasdale R.D., Tomita M., RA Verardo R., Wagner L., Wahlestedt C., Wang Y., Watanabe Y., Wells C., RA Wilming L.G., Wynshaw-Boris A., Yanagisawa M., Yang I., Yang L., RA Yuan Z., Zavolan M., Zhu Y., Zimmer A., Carninci P., Hayatsu N., RA Hirozane-Kishikawa T., Konno H., Nakamura M., Sakazume N., Sato K., RA Shiraki T., Waki K., Kawai J., Aizawa K., Arakawa T., Fukuda S., RA Hara A., Hashizume W., Imotani K., Ishii Y., Itoh M., Kagawa I., RA Miyazaki A., Sakai K., Sasaki D., Shibata K., Shinagawa A., RA Yasunishi A., Yoshino M., Waterston R., Lander E.S., Rogers J., RA Birney E., Hayashizaki Y.; RT "Analysis of the mouse transcriptome based on functional annotation of RT 60,770 full-length cDNAs."; RL Nature 420:563-573(2002). RN [2] RP SEQUENCE FROM N.A. (ISOFORM 4), AND VARIANTS. RC STRAIN=C57BL/6J; TISSUE=Spleen; RA Mariani R., Landau N.R.; RL Submitted (FEB-2004) to Swiss-Prot. RN [3] RP SEQUENCE FROM N.A. RX PubMed=12466850; DOI=10.1038/nature01262; RA Waterston R.H., Lindblad-Toh K., Birney E., Rogers J., Abril J.F., RA Agarwal P., Agarwala R., Ainscough R., Alexandersson M., An P., RA Antonarakis S.E., Attwood J., Baertsch R., Bailey J., Barlow K., RA Beck S., Berry E., Birren B., Bloom T., Bork P., Botcherby M., RA Bray N., Brent M.R., Brown D.G., Brown S.D., Bult C., Burton J., RA Butler J., Campbell R.D., Carninci P., Cawley S., Chiaromonte F., RA Chinwalla A.T., Church D.M., Clamp M., Clee C., Collins F.S., RA Cook L.L., Copley R.R., Coulson A., Couronne O., Cuff J., Curwen V., RA Cutts T., Daly M., David R., Davies J., Delehaunty K.D., Deri J., RA Dermitzakis E.T., Dewey C., Dickens N.J., Diekhans M., Dodge S., RA Dubchak I., Dunn D.M., Eddy S.R., Elnitski L., Emes R.D., Eswara P., RA Eyras E., Felsenfeld A., Fewell G.A., Flicek P., Foley K., RA Frankel W.N., Fulton L.A., Fulton R.S., Furey T.S., Gage D., RA Gibbs R.A., Glusman G., Gnerre S., Goldman N., Goodstadt L., RA Grafham D., Graves T.A., Green E.D., Gregory S., Guigo R., Guyer M., RA Hardison R.C., Haussler D., Hayashizaki Y., Hillier L.W., Hinrichs A., RA Hlavina W., Holzer T., Hsu F., Hua A., Hubbard T., Hunt A., RA Jackson I., Jaffe D.B., Johnson L.S., Jones M., Jones T.A., Joy A., RA Kamal M., Karlsson E.K., Karolchik D., Kasprzyk A., Kawai J., RA Keibler E., Kells C., Kent W.J., Kirby A., Kolbe D.L., Korf I., RA Kucherlapati R.S., Kulbokas E.J., Kulp D., Landers T., Leger J.P., RA Leonard S., Letunic I., Levine R., Li J., Li M., Lloyd C., Lucas S., RA Ma B., Maglott D.R., Mardis E.R., Matthews L., Mauceli E., Mayer J.H., RA McCarthy M., McCombie W.R., McLaren S., McLay K., McPherson J.D., RA Meldrim J., Meredith B., Mesirov J.P., Miller W., Miner T.L., RA Mongin E., Montgomery K.T., Morgan M., Mott R., Mullikin J.C., RA Muzny D.M., Nash W.E., Nelson J.O., Nhan M.N., Nicol R., Ning Z., RA Nusbaum C., O'Connor M.J., Okazaki Y., Oliver K., Overton-Larty E., RA Pachter L., Parra G., Pepin K.H., Peterson J., Pevzner P., Plumb R., RA Pohl C.S., Poliakov A., Ponce T.C., Ponting C.P., Potter S., Quail M., RA Reymond A., Roe B.A., Roskin K.M., Rubin E.M., Rust A.G., Santos R., RA Sapojnikov V., Schultz B., Schultz J., Schwartz M.S., Schwartz S., RA Scott C., Seaman S., Searle S., Sharpe T., Sheridan A., Shownkeen R., RA Sims S., Singer J.B., Slater G., Smit A., Smith D.R., Spencer B., RA Stabenau A., Stange-Thomann N., Sugnet C., Suyama M., Tesler G., RA Thompson J., Torrents D., Trevaskis E., Tromp J., Ucla C., RA Ureta-Vidal A., Vinson J.P., Von Niederhausern A.C., Wade C.M., RA Wall M., Weber R.J., Weiss R.B., Wendl M.C., West A.P., RA Wetterstrand K., Wheeler R., Whelan S., Wierzbowski J., Willey D., RA Williams S., Wilson R.K., Winter E., Worley K.C., Wyman D., Yang S., RA Yang S.P., Zdobnov E.M., Zody M.C., Lander E.S.; RT "Initial sequencing and comparative analysis of the mouse genome."; RL Nature 420:520-562(2002). RN [4] RP SEQUENCE FROM N.A. (ISOFORM 1), AND VARIANTS ARG-34; 37-ILE-ASP-38; RP 112-VAL-LEU-113; 134-ILE-ARG-135; ASN-139; LYS-181; RP 233-VAL-HIS-LEU-235; GLY-258; ILE-306 AND HIS-404. RC TISSUE=Breast tumor; RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human RT and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [5] RP DNA C TO U EDITING ACTIVITY, AND TISSUE SPECIFICITY. RX MEDLINE=22744372; PubMed=12859895; DOI=10.1016/S0092-8674(03)00515-4; RA Mariani R., Chen D., Schroefelbauer B., Navarro F., Koenig R., RA Bollman B., Muenk C., Nymark-McMahon H., Landau N.R.; RT "Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif."; RL Cell 114:21-31(2003). CC -!- FUNCTION: Probable human APOBEC3G ortholog. Has DNA deaminase CC (cytidine deaminase) activity. Mediates G-to-A hypermutation in CC newly synthetized HIV-1 viral DNA and is thus able to prevent HIV- CC 1 infectivity in vitro, in the presence and absence of HIV-1 VIF CC (virion infectivity factor). CC -!- COFACTOR: Zinc (By similarity). CC -!- SUBCELLULAR LOCATION: Mainly cytoplasmic. Small amount are found CC in the nucleus (By similarity). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=4; CC Name=1; CC IsoId=Q99J72-1; Sequence=Displayed; CC Name=2; CC IsoId=Q99J72-2; Sequence=VSP_009832; CC Name=3; CC IsoId=Q99J72-3; Sequence=VSP_009831, VSP_009833; CC Name=4; CC IsoId=Q99J72-4; Sequence=VSP_009831; CC Note=Lacks exon V. Also active in inhibiting HIV-1 in vitro; CC -!- TISSUE SPECIFICITY: Expressed in spleen, node and lung. CC -!- POLYMORPHISM: Polymorphism seems to be an important factor for the CC ability of the protein to prevent HIV-1 infectivity in vitro. The CC protein derived from MDTF and EL4 cell lines are unable to reduce CC HIV-1 infectivity, while the protein derived from L1.2, 3T3 and CC splenocytes are able to do it, in the presence and absence of HIV- CC 1 VIF (virion infectivity factor). CC -!- MISCELLANEOUS: HIV-1 VIF (virion infectivity factor) does not bind CC to mouse APOBEC3. CC -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase CC family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK044394; BAC31901.1; -. DR EMBL; AK049998; BAC34023.1; -. DR EMBL; AC113595; -; NOT_ANNOTATED_CDS. DR EMBL; BC003314; AAH03314.1; -. DR MGD; MGI:1933111; Apobec3. DR InterPro; IPR007904; APOBEC_C. DR InterPro; IPR002125; dCMP/cyt_deam. DR Pfam; PF05240; APOBEC_C; 2. DR PROSITE; PS00903; CYT_DCMP_DEAMINASES; 2. KW Alternative splicing; Hydrolase; Nuclear protein; Polymorphism; Zinc. FT METAL 71 71 Zinc (By similarity). FT METAL 105 105 Zinc (By similarity). FT METAL 108 108 Zinc (By similarity). FT METAL 288 288 Zinc (By similarity). FT METAL 316 316 Zinc (By similarity). FT METAL 319 319 Zinc (By similarity). FT VARSPLIC 198 230 Missing (in isoform 3 and isoform 4). FT /FTId=VSP_009831. FT VARSPLIC 409 429 SWGLQDLVNDFGNLQLGPPMS -> VRTTLLQGPAS (in FT isoform 2). FT /FTId=VSP_009832. FT VARSPLIC 409 429 SWGLQDLVNDFGNLQLGPPMS -> SRSHAS (in FT isoform 3). FT /FTId=VSP_009833. FT VARIANT 20 20 L -> V (in cell line MDTF). FT VARIANT 31 31 K -> E (in cell line MDTF). FT VARIANT 34 34 G -> R (in cell lines L1.2 and 3T3). FT VARIANT 34 35 GY -> PF (in cell line MDTF). FT VARIANT 37 38 KG -> ID (in cell lines L1.2 and 3T3). FT VARIANT 38 38 G -> K (in cell line MDTF). FT VARIANT 111 111 Q -> R (in cell line L1.2). FT VARIANT 112 112 I -> V (in cell line MDTF). FT VARIANT 112 113 IV -> VL (in cell lines L1.2 and 3T3). FT VARIANT 128 128 S -> F (in cell line MDTF). FT VARIANT 134 139 VQDPET -> IRNPEN (in cell line MDTF). FT VARIANT 134 135 VQ -> IR (in cell lines L1.2 and 3T3). FT VARIANT 139 139 T -> N (in cell line L1.2). FT VARIANT 139 140 TQ -> NQL (in cell line 3T3). FT VARIANT 148 148 Q -> L (in cell line MDTF). FT VARIANT 162 163 KK -> EE (in cell line MDTF). FT VARIANT 181 181 R -> K (in cell lines L1.2 and 3T3). FT VARIANT 198 230 Missing (in cell line MDTF). FT VARIANT 230 235 GRRMDP -> RRRVHL (in cell line 3T3). FT VARIANT 233 235 MDP -> VHL. FT VARIANT 234 235 DP -> SL (in cell line MDTF). FT VARIANT 248 248 Q -> L (in cell line MDTF). FT VARIANT 258 258 R -> G (in cell line L1.2). FT VARIANT 258 259 RM -> GV (in cell line 3T3). FT VARIANT 264 264 C -> F (in cell line MDTF). FT VARIANT 269 269 Q -> W (in cell line MDTF). FT VARIANT 274 274 A -> E (in cell line MDTF). FT VARIANT 284 296 Missing (in cell line MDTF). FT VARIANT 306 306 T -> I (in cell lines L1.2 and 3T3). FT VARIANT 314 314 S -> G (in cell line EL4). FT VARIANT 328 328 R -> K (in cell line MDTF). FT VARIANT 381 381 N -> S (in cell line MDTF). FT VARIANT 387 387 W -> R (in splenocytes). FT VARIANT 394 395 II -> KT (in cell line MDTF). FT VARIANT 399 399 T -> A (in cell line L1.2). FT VARIANT 404 404 R -> C (in cell lines MDTF and 3T3). FT VARIANT 404 404 R -> H (in cell line L1.2). FT CONFLICT 203 203 P -> S (in Ref. 3). FT CONFLICT 227 227 C -> W (in Ref. 3). FT CONFLICT 230 230 G -> R (in Ref. 2). FT CONFLICT 410 414 WGLQD -> RSHAS (in Ref. 1; BAC34023). SQ SEQUENCE 429 AA; 50948 MW; 2B4361CDD81C99E5 CRC64; MGPFCLGCSH RKCYSPIRNL ISQETFKFHF KNLGYAKGRK DTFLCYEVTR KDCDSPVSLH HGVFKNKDNI HAEICFLYWF HDKVLKVLSP REEFKITWYM SWSPCFECAE QIVRFLATHH NLSLDIFSSR LYNVQDPETQ QNLCRLVQEG AQVAAMDLYE FKKCWKKFVD NGGRRFRPWK RLLTNFRYQD SKLQEILRPC YIPVPSSSSS TLSNICLTKG LPETRFCVEG RRMDPLSEEE FYSQFYNQRV KHLCYYHRMK PYLCYQLEQF NGQAPLKGCL LSEKGKQHAE ILFLDKIRSM ELSQVTITCY LTWSPCPNCA WQLAAFKRDR PDLILHIYTS RLYFHWKRPF QKGLCSLWQS GILVDVMDLP QFTDCWTNFV NPKRPFWPWK GLEIISRRTQ RRLRRIKESW GLQDLVNDFG NLQLGPPMS //