ID   CGRF1_HUMAN             Reviewed;         332 AA.
AC   Q99675; Q96BX2;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   13-FEB-2019, entry version 146.
DE   RecName: Full=Cell growth regulator with RING finger domain protein 1;
DE   AltName: Full=Cell growth regulatory gene 19 protein;
DE   AltName: Full=RING finger protein 197;
GN   Name=CGRRF1; Synonyms=CGR19, RNF197;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Fetal brain;
RX   PubMed=8968090;
RA   Madden S.L., Galella E.A., Riley D., Bertelsen A.H., Beaudry G.A.;
RT   "Induction of cell growth regulatory genes by p53.";
RL   Cancer Res. 56:5384-5390(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Skeletal muscle;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=22361696; DOI=10.1016/j.jprot.2012.01.030;
RA   Stadler C., Hjelmare M., Neumann B., Jonasson K., Pepperkok R.,
RA   Uhlen M., Lundberg E.;
RT   "Systematic validation of antibody binding and protein subcellular
RT   localization using siRNA and confocal microscopy.";
RL   J. Proteomics 75:2236-2251(2012).
RN   [4]
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=27485036; DOI=10.1038/srep30955;
RA   Kaneko M., Iwase I., Yamasaki Y., Takai T., Wu Y., Kanemoto S.,
RA   Matsuhisa K., Asada R., Okuma Y., Watanabe T., Imaizumi K., Nomura Y.;
RT   "Genome-wide identification and gene expression profiling of ubiquitin
RT   ligases for endoplasmic reticulum protein degradation.";
RL   Sci. Rep. 6:30955-30955(2016).
RN   [5]
RP   STRUCTURE BY NMR OF 262-318.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Solution structure of the RING domain of the human cell growth
RT   regulator with RING finger domain 1 protein.";
RL   Submitted (JUL-2007) to the PDB data bank.
CC   -!- FUNCTION: Able to inhibit growth in several cell lines.
CC       {ECO:0000250|UniProtKB:P97587}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22361696}.
CC       Endoplasmic reticulum {ECO:0000269|PubMed:27485036}.
CC   -!- TISSUE SPECIFICITY: Ubiquitously expressed with high expression in
CC       testis and the cerebellum. {ECO:0000269|PubMed:27485036}.
CC   -!- INDUCTION: Up-regulated by endoplasmic reticulum (ER) stress
CC       triggered by thapsigargin or tunicamycin.
CC       {ECO:0000269|PubMed:27485036}.
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DR   EMBL; U66469; AAC50897.1; -; mRNA.
DR   EMBL; BC015063; AAH15063.1; -; mRNA.
DR   CCDS; CCDS9719.1; -.
DR   RefSeq; NP_006559.1; NM_006568.2.
DR   UniGene; Hs.59106; -.
DR   PDB; 2EA5; NMR; -; A=264-318.
DR   PDBsum; 2EA5; -.
DR   ProteinModelPortal; Q99675; -.
DR   SMR; Q99675; -.
DR   BioGrid; 115910; 42.
DR   IntAct; Q99675; 19.
DR   MINT; Q99675; -.
DR   STRING; 9606.ENSP00000216420; -.
DR   iPTMnet; Q99675; -.
DR   PhosphoSitePlus; Q99675; -.
DR   BioMuta; CGRRF1; -.
DR   DMDM; 44887778; -.
DR   EPD; Q99675; -.
DR   MaxQB; Q99675; -.
DR   PaxDb; Q99675; -.
DR   PeptideAtlas; Q99675; -.
DR   PRIDE; Q99675; -.
DR   ProteomicsDB; 78389; -.
DR   DNASU; 10668; -.
DR   Ensembl; ENST00000216420; ENSP00000216420; ENSG00000100532.
DR   GeneID; 10668; -.
DR   KEGG; hsa:10668; -.
DR   UCSC; uc001xay.4; human.
DR   CTD; 10668; -.
DR   DisGeNET; 10668; -.
DR   EuPathDB; HostDB:ENSG00000100532.11; -.
DR   GeneCards; CGRRF1; -.
DR   HGNC; HGNC:15528; CGRRF1.
DR   HPA; HPA002930; -.
DR   MIM; 606138; gene.
DR   neXtProt; NX_Q99675; -.
DR   OpenTargets; ENSG00000100532; -.
DR   PharmGKB; PA134985671; -.
DR   eggNOG; ENOG410ITZ9; Eukaryota.
DR   eggNOG; ENOG4111F4I; LUCA.
DR   GeneTree; ENSGT00390000004542; -.
DR   HOGENOM; HOG000111607; -.
DR   HOVERGEN; HBG050932; -.
DR   InParanoid; Q99675; -.
DR   OMA; CDGCVRY; -.
DR   OrthoDB; 670633at2759; -.
DR   PhylomeDB; Q99675; -.
DR   TreeFam; TF328102; -.
DR   ChiTaRS; CGRRF1; human.
DR   EvolutionaryTrace; Q99675; -.
DR   GenomeRNAi; 10668; -.
DR   PRO; PR:Q99675; -.
DR   Proteomes; UP000005640; Chromosome 14.
DR   Bgee; ENSG00000100532; Expressed in 229 organ(s), highest expression level in testis.
DR   ExpressionAtlas; Q99675; baseline and differential.
DR   Genevisible; Q99675; HS.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007050; P:cell cycle arrest; IEA:UniProtKB-KW.
DR   GO; GO:0008285; P:negative regulation of cell population proliferation; TAS:ProtInc.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   SMART; SM00184; RING; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell cycle; Complete proteome; Endoplasmic reticulum;
KW   Growth arrest; Metal-binding; Nucleus; Polymorphism;
KW   Reference proteome; Zinc; Zinc-finger.
FT   CHAIN         1    332       Cell growth regulator with RING finger
FT                                domain protein 1.
FT                                /FTId=PRO_0000055869.
FT   ZN_FING     274    309       RING-type. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00175}.
FT   VARIANT     117    117       C -> Y (in dbSNP:rs11555279).
FT                                /FTId=VAR_052081.
FT   CONFLICT    103    103       S -> N (in Ref. 2; AAH15063).
FT                                {ECO:0000305}.
FT   STRAND      275    280       {ECO:0000244|PDB:2EA5}.
FT   TURN        287    290       {ECO:0000244|PDB:2EA5}.
FT   HELIX       298    301       {ECO:0000244|PDB:2EA5}.
FT   TURN        306    308       {ECO:0000244|PDB:2EA5}.
SQ   SEQUENCE   332 AA;  38242 MW;  2F1FC0D12B710C80 CRC64;
     MAAVFLVTLY EYSPLFYIAV VFTCFIVTTG LVLGWFGWDV PVILRNSEET QFSTRVFKKQ
     MRQVKNPFGL EITNPSSASI TTGITLTTDC LEDSLLTCYW GCSVQKLYEA LQKHVYCFRI
     STPQALEDAL YSEYLYQEQY FIKKDSKEEI YCQLPRDTKI EDFGTVPRSR YPLVALLTLA
     DEDDREIYDI ISMVSVIHIP DRTYKLSCRI LYQYLLLAQG QFHDLKQLFM SANNNFTPSN
     NSSSEEKNTD RSLLEKVGLS ESEVEPSEEN SKDCVVCQNG TVNWVLLPCR HTCLCDGCVK
     YFQQCPMCRQ FVQESFALCS QKEQDKDKPK TL
//