ID   SPSB2_HUMAN             Reviewed;         263 AA.
AC   Q99619; D3DUT0;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   08-FEB-2011, entry version 85.
DE   RecName: Full=SPRY domain-containing SOCS box protein 2;
DE            Short=SSB-2;
DE   AltName: Full=Gene-rich cluster protein C9;
GN   Name=SPSB2; Synonyms=GRCC9, SSB2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=97228904; PubMed=9074930;
RA   Ansari-Lari M.A., Shen Y., Muzny D.M., Lee W., Gibbs R.A.;
RT   "Large-scale sequencing in human chromosome 12p13: experimental and
RT   computational gene structure determination.";
RL   Genome Res. 7:268-280(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Friedel E.J., Nicholson S.E., Nicola N.A., Kile B.T., Hilton D.J.;
RT   "SOCS box proteins.";
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta, and Spleen;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   FUNCTION IN AN E3 UBIQUITIN-PROTEIN LIGASE COMPLEX, MASS SPECTROMETRY,
RP   AND INTERACTION WITH CUL5; RNF7; TCEB1 AND TCEB2.
RX   PubMed=15601820; DOI=10.1101/gad.1252404;
RA   Kamura T., Maenaka K., Kotoshiba S., Matsumoto M., Kohda D.,
RA   Conaway R.C., Conaway J.W., Nakayama K.I.;
RT   "VHL-box and SOCS-box domains determine binding specificity for Cul2-
RT   Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.";
RL   Genes Dev. 18:3055-3065(2004).
RN   [6]
RP   INTERACTION WITH MET.
RX   PubMed=15713673; DOI=10.1074/jbc.M413897200;
RA   Wang D., Li Z., Messing E.M., Wu G.;
RT   "The SPRY domain-containing SOCS box protein 1 (SSB-1) interacts with
RT   MET and enhances the hepatocyte growth factor-induced Erk-Elk-1-serum
RT   response element pathway.";
RL   J. Biol. Chem. 280:16393-16401(2005).
CC   -!- FUNCTION: Probable substrate recognition component of a SCF-like
CC       ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein
CC       ligase complex which mediates the ubiquitination and subsequent
CC       proteasomal degradation of target proteins.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Interacts with PARW (By similarity). Component of the
CC       probable ECS(SPSB2) E3 ubiquitin-protein ligase complex which
CC       contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB2. Interacts
CC       with CUL5, RNF7, TCEB1 and TCEB2. Interacts with MET.
CC   -!- INTERACTION:
CC       Q15369:TCEB1; NbExp=1; IntAct=EBI-2323209, EBI-301231;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- DOMAIN: The SOCS box domain mediates the interaction with the
CC       Elongin BC complex, an adapter module in different E3 ubiquitin
CC       ligase complexes (By similarity).
CC   -!- SIMILARITY: Belongs to the SPSB family.
CC   -!- SIMILARITY: Contains 1 B30.2/SPRY domain.
CC   -!- SIMILARITY: Contains 1 SOCS box domain.
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DR   EMBL; U47924; AAB51328.1; -; Genomic_DNA.
DR   EMBL; AF403027; AAL57346.1; -; mRNA.
DR   EMBL; CH471116; EAW88719.1; -; Genomic_DNA.
DR   EMBL; CH471116; EAW88720.1; -; Genomic_DNA.
DR   EMBL; BC002983; AAH02983.1; -; mRNA.
DR   EMBL; BC071933; AAH71933.1; -; mRNA.
DR   IPI; IPI00013315; -.
DR   RefSeq; NP_001139788.1; NM_001146316.1.
DR   RefSeq; NP_116030.1; NM_032641.3.
DR   UniGene; Hs.479856; -.
DR   PDB; 3EMW; X-ray; 1.80 A; A=26-219.
DR   PDBsum; 3EMW; -.
DR   ProteinModelPortal; Q99619; -.
DR   SMR; Q99619; 24-219.
DR   IntAct; Q99619; 9.
DR   STRING; Q99619; -.
DR   PRIDE; Q99619; -.
DR   GeneID; 84727; -.
DR   KEGG; hsa:84727; -.
DR   UCSC; uc001qrl.1; human.
DR   CTD; 84727; -.
DR   GeneCards; GC12M006980; -.
DR   H-InvDB; HIX0010384; -.
DR   HGNC; HGNC:29522; SPSB2.
DR   MIM; 611658; gene.
DR   neXtProt; NX_Q99619; -.
DR   PharmGKB; PA142670872; -.
DR   eggNOG; maNOG13004; -.
DR   GeneTree; ENSGT00390000009402; -.
DR   HOVERGEN; HBG055793; -.
DR   InParanoid; Q99619; -.
DR   PhylomeDB; Q99619; -.
DR   NextBio; 74846; -.
DR   CleanEx; HS_SPSB2; -.
DR   Genevestigator; Q99619; -.
DR   GermOnline; ENSG00000111671; Homo sapiens.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR001870; B302/SPRY.
DR   InterPro; IPR008985; ConA-like_lec_gl.
DR   InterPro; IPR001496; SOCS_C.
DR   InterPro; IPR018355; SPla/RYanodine_receptor_sg.
DR   InterPro; IPR003877; SPRY_rcpt.
DR   Pfam; PF07525; SOCS_box; 1.
DR   Pfam; PF00622; SPRY; 1.
DR   SMART; SM00969; SOCS_box; 1.
DR   SMART; SM00449; SPRY; 1.
DR   SUPFAM; SSF49899; ConA_like_lec_gl; 1.
DR   PROSITE; PS50188; B302_SPRY; 1.
DR   PROSITE; PS50225; SOCS; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Cytoplasm; Ubl conjugation pathway.
FT   CHAIN         1    263       SPRY domain-containing SOCS box protein
FT                                2.
FT                                /FTId=PRO_0000238474.
FT   DOMAIN       26    221       B30.2/SPRY.
FT   DOMAIN      222    263       SOCS box.
FT   HELIX        30     34
FT   TURN         43     45
FT   STRAND       57     60
FT   STRAND       65     68
FT   STRAND       71     73
FT   STRAND       89     94
FT   HELIX        97     99
FT   STRAND      117    120
FT   STRAND      165    171
FT   STRAND      189    191
FT   STRAND      211    216
SQ   SEQUENCE   263 AA;  28630 MW;  0729EDDD0B598665 CRC64;
     MGQTALAGGS SSTPTPQALY PDLSCPEGLE ELLSAPPPDL GAQRRHGWNP KDCSENIEVK
     EGGLYFERRP VAQSTDGARG KRGYSRGLHA WEISWPLEQR GTHAVVGVAT ALAPLQTDHY
     AALLGSNSES WGWDIGRGKL YHQSKGPGAP QYPAGTQGEQ LEVPERLLVV LDMEEGTLGY
     AIGGTYLGPA FRGLKGRTLY PAVSAVWGQC QVRIRYLGER RAEPHSLLHL SRLCVRHNLG
     DTRLGQVSAL PLPPAMKRYL LYQ
//