ID   COMT_PIG       STANDARD;      PRT;   186 AA.
AC   Q99028;
DT   01-JUN-1994 (Rel. 29, Created)
DT   01-JUN-1994 (Rel. 29, Last sequence update)
DT   15-DEC-1998 (Rel. 37, Last annotation update)
DE   CATECHOL-O-METHYLTRANSFERASE, SOLUBLE FORM (EC 2.1.1.6) (S-COMT)
DE   (FRAGMENT).
GN   COMT.
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia;
OC   Eutheria; Cetartiodactyla; Suina; Suidae; Sus.
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE; 91142183.
RA   BERTOCCI B., MIGGIANO V., DA PRADA M., DEMBIC Z., LAHM H-.W.,
RA   MALHERBE P.;
RT   "Human catechol-O-methyltransferase: cloning and expression of the
RT   membrane-associated form.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:1416-1420(1991).
RN   [2]
RP   PARTIAL SEQUENCE.
RC   TISSUE=LIVER;
RX   MEDLINE; 92031656.
RA   BERTOCCI B., GAROTTA G., DA PRADA M., LAHM H-.W., ZURCHER G.,
RA   VIRGALLITA G., MIGGIANO V.;
RT   "Immunoaffinity purification and partial amino acid sequence analysis
RT   of catechol-O-methyltransferase from pig liver.";
RL   Biochim. Biophys. Acta 1080:103-109(1991).
CC   -!- FUNCTION: CATALYZES THE O-METHYLATION, AND THEREBY THE
CC       INACTIVATION, OF CATECHOLAMINE NEUROTRANSMITTERS AND CATECHOL
CC       HORMONES. ALSO SHORTENS THE BIOLOGICAL HALF-LIVES OF CERTAIN
CC       NEUROACTIVE DRUGS, LIKE L-DOPA, ALPHA-METHYL DOPA AND
CC       ISOPROTERENOL.
CC   -!- CATALYTIC ACTIVITY: S-ADENOSYL-L-METHIONINE + CATECHOL =
CC       S-ADENOSYL-L-HOMOCYSTEINE + GUAIACOL.
CC   -!- COFACTOR: MAGNESIUM.
CC   -!- SUBCELLULAR LOCATION: OCCURS IN TWO FORMS: CYTOSOLIC (S-COMT),
CC       AND MEMBRANE-BOUND (MB-COMT).
CC   -!- SIMILARITY: TO OTHER MAMMALIAN CATECHOL-O-METHYLTRANSFERASE.
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DR   HSSP; P22734; 1VID.
KW   Transferase; Methyltransferase; Neurotransmitter degradation;
KW   Magnesium.
FT   NON_TER       1      1
FT   ACT_SITE    109    109       BY SIMILARITY.
FT   ACT_SITE    164    164       BY SIMILARITY.
FT   METAL       106    106       MAGNESIUM (BY SIMILARITY).
FT   METAL       134    134       MAGNESIUM (BY SIMILARITY).
FT   METAL       135    135       MAGNESIUM (BY SIMILARITY).
SQ   SEQUENCE   186 AA;  20587 MW;  88AD90FB CRC32;
     KERAMHVGRK KGQIVDTVVQ EQRPSVLLEL GAYCGYSAVR MARLLLPSAR LLTIELNPDN
     AAIAQQVVDF AGLQDRVTVV VGASQDIIPQ LKKKYDVDTL DMVFLDHWKD RYLPDTLLLE
     ECGLLRKGTV LLADNVICPG APDFLAHVRG CGRFECTHFS SYLEYSQMVD GLEKAVYKGP
     GSPAQP
//