ID   COMT_PIG       STANDARD;      PRT;   186 AA.
AC   Q99028;
DT   01-JUN-1994 (Rel. 29, Created)
DT   01-JUN-1994 (Rel. 29, Last sequence update)
DT   13-SEP-2005 (Rel. 48, Last annotation update)
DE   Catechol O-methyltransferase (EC 2.1.1.6) (Fragment).
GN   Name=COMT;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
OC   Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=91142183; PubMed=1847521;
RA   Bertocci B., Miggiano V., da Prada M., Dembic Z., Lahm H.-W.,
RA   Malherbe P.;
RT   "Human catechol-O-methyltransferase: cloning and expression of the
RT   membrane-associated form.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:1416-1420(1991).
RN   [2]
RP   PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Liver;
RX   MEDLINE=92031656; PubMed=1932084; DOI=10.1016/0167-4838(91)90135-M;
RA   Bertocci B., Garotta G., da Prada M., Lahm H.-W., Zurcher G.,
RA   Virgallita G., Miggiano V.;
RT   "Immunoaffinity purification and partial amino acid sequence analysis
RT   of catechol-O-methyltransferase from pig liver.";
RL   Biochim. Biophys. Acta 1080:103-109(1991).
CC   -!- FUNCTION: Catalyzes the O-methylation, and thereby the
CC       inactivation, of catecholamine neurotransmitters and catechol
CC       hormones. Also shortens the biological half-lives of certain
CC       neuroactive drugs, like L-DOPA, alpha-methyl DOPA and
CC       isoproterenol.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + a catechol = S-
CC       adenosyl-L-homocysteine + a guaiacol.
CC   -!- COFACTOR: Magnesium.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic (isoform S-COMT). Type II
CC       membrane protein (isoform MB-COMT).
CC   -!- SIMILARITY: Belongs to the mammalian catechol-O-methyltransferase
CC       family.
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DR   HSSP; P22734; 1VID.
DR   SMR; Q99028; 1-180.
DR   InterPro; IPR002935; Methyltransf_3.
DR   InterPro; IPR000051; SAM_bd.
DR   PANTHER; PTHR10509; Methyltransf_3; 1.
DR   Pfam; PF01596; Methyltransf_3; 1.
KW   Catecholamine metabolism; Direct protein sequencing; Magnesium;
KW   Metal-binding; Methyltransferase; Neurotransmitter degradation;
KW   Transferase.
FT   ACT_SITE    109    109       By similarity.
FT   ACT_SITE    164    164       By similarity.
FT   METAL       106    106       Magnesium (By similarity).
FT   METAL       134    134       Magnesium (By similarity).
FT   METAL       135    135       Magnesium (By similarity).
FT   NON_TER       1      1
SQ   SEQUENCE   186 AA;  20587 MW;  493B8801F06C6262 CRC64;
     KERAMHVGRK KGQIVDTVVQ EQRPSVLLEL GAYCGYSAVR MARLLLPSAR LLTIELNPDN
     AAIAQQVVDF AGLQDRVTVV VGASQDIIPQ LKKKYDVDTL DMVFLDHWKD RYLPDTLLLE
     ECGLLRKGTV LLADNVICPG APDFLAHVRG CGRFECTHFS SYLEYSQMVD GLEKAVYKGP
     GSPAQP
//