ID   COMT_PIG       STANDARD;      PRT;   186 AA.
AC   Q99028;
DT   01-JUN-1994 (Rel. 29, Created)
DT   01-JUN-1994 (Rel. 29, Last sequence update)
DT   01-OCT-2000 (Rel. 40, Last annotation update)
DE   CATECHOL-O-METHYLTRANSFERASE, SOLUBLE FORM (EC 2.1.1.6) (S-COMT)
DE   (FRAGMENT).
GN   COMT.
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Cetartiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE=91142183; PubMed=1847521;
RA   Bertocci B., Miggiano V., da Prada M., Dembic Z., Lahm H.-W.,
RA   Malherbe P.;
RT   "Human catechol-O-methyltransferase: cloning and expression of the
RT   membrane-associated form.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:1416-1420(1991).
RN   [2]
RP   PARTIAL SEQUENCE.
RC   TISSUE=Liver;
RX   MEDLINE=92031656; PubMed=1932084;
RA   Bertocci B., Garotta G., da Prada M., Lahm H.-W., Zurcher G.,
RA   Virgallita G., Miggiano V.;
RT   "Immunoaffinity purification and partial amino acid sequence analysis
RT   of catechol-O-methyltransferase from pig liver.";
RL   Biochim. Biophys. Acta 1080:103-109(1991).
CC   -!- FUNCTION: CATALYZES THE O-METHYLATION, AND THEREBY THE
CC       INACTIVATION, OF CATECHOLAMINE NEUROTRANSMITTERS AND CATECHOL
CC       HORMONES. ALSO SHORTENS THE BIOLOGICAL HALF-LIVES OF CERTAIN
CC       NEUROACTIVE DRUGS, LIKE L-DOPA, ALPHA-METHYL DOPA AND
CC       ISOPROTERENOL.
CC   -!- CATALYTIC ACTIVITY: S-ADENOSYL-L-METHIONINE + CATECHOL =
CC       S-ADENOSYL-L-HOMOCYSTEINE + GUAIACOL.
CC   -!- COFACTOR: MAGNESIUM.
CC   -!- SUBCELLULAR LOCATION: OCCURS IN TWO FORMS: CYTOSOLIC (S-COMT),
CC       AND MEMBRANE-BOUND (MB-COMT).
CC   -!- SIMILARITY: TO OTHER MAMMALIAN CATECHOL-O-METHYLTRANSFERASE.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   HSSP; P22734; 1VID.
DR   InterPro; IPR000051; SAM_bind.
KW   Transferase; Methyltransferase; Neurotransmitter degradation;
KW   Catecholamine metabolism; Magnesium.
FT   NON_TER       1      1
FT   ACT_SITE    109    109       BY SIMILARITY.
FT   ACT_SITE    164    164       BY SIMILARITY.
FT   METAL       106    106       MAGNESIUM (BY SIMILARITY).
FT   METAL       134    134       MAGNESIUM (BY SIMILARITY).
FT   METAL       135    135       MAGNESIUM (BY SIMILARITY).
SQ   SEQUENCE   186 AA;  20587 MW;  493B8801F06C6262 CRC64;
     KERAMHVGRK KGQIVDTVVQ EQRPSVLLEL GAYCGYSAVR MARLLLPSAR LLTIELNPDN
     AAIAQQVVDF AGLQDRVTVV VGASQDIIPQ LKKKYDVDTL DMVFLDHWKD RYLPDTLLLE
     ECGLLRKGTV LLADNVICPG APDFLAHVRG CGRFECTHFS SYLEYSQMVD GLEKAVYKGP
     GSPAQP
//