ID   JMJD8_HUMAN             Reviewed;         264 AA.
AC   Q96S16; B2RNS7; D3DU58; Q4PKE3; Q4VBY1; Q6GMW5; Q71RB8;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2018, sequence version 2.
DT   24-JAN-2024, entry version 131.
DE   RecName: Full=JmjC domain-containing protein 8 {ECO:0000305};
DE   AltName: Full=Jumonji domain-containing protein 8;
DE   Flags: Precursor;
GN   Name=JMJD8 {ECO:0000312|HGNC:HGNC:14148};
GN   Synonyms=C16orf20 {ECO:0000312|HGNC:HGNC:14148}; ORFNames=PP14397;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA   Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA   Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA   Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT   "Large-scale cDNA transfection screening for genes related to cancer
RT   development and progression.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA   Li H., Nong W., Zhou G., Ke R., Shen C., Zhong G., Zheng Z., Liang M.,
RA   Wen S., Lin L., Yang S.;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11157797; DOI=10.1093/hmg/10.4.339;
RA   Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C.,
RA   Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R.;
RT   "Sequence, structure and pathology of the fully annotated terminal 2 Mb of
RT   the short arm of human chromosome 16.";
RL   Hum. Mol. Genet. 10:339-352(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15616553; DOI=10.1038/nature03187;
RA   Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA   Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA   Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA   Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA   Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA   Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA   Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA   Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA   Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA   Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA   Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA   Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA   Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA   Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA   Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA   Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA   Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA   Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA   Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA   DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA   Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA   Myers R.M., Rubin E.M., Pennacchio L.A.;
RT   "The sequence and analysis of duplication-rich human chromosome 16.";
RL   Nature 432:988-994(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Kidney, and Uterus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   INTERACTION WITH PKM, SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=27199445; DOI=10.1161/atvbaha.116.307695;
RA   Boeckel J.N., Derlet A., Glaser S.F., Luczak A., Lucas T., Heumueller A.W.,
RA   Krueger M., Zehendner C.M., Kaluza D., Doddaballapur A., Ohtani K.,
RA   Treguer K., Dimmeler S.;
RT   "JMJD8 Regulates Angiogenic Sprouting and Cellular Metabolism by
RT   Interacting With Pyruvate Kinase M2 in Endothelial Cells.";
RL   Arterioscler. Thromb. Vasc. Biol. 36:1425-1433(2016).
RN   [8]
RP   FUNCTION.
RX   PubMed=27671354; DOI=10.1038/srep34125;
RA   Yeo K.S., Tan M.C., Wong W.Y., Loh S.W., Lam Y.L., Tan C.L., Lim Y.Y.,
RA   Ea C.K.;
RT   "JMJD8 is a positive regulator of TNF-induced NF-kappaB signaling.";
RL   Sci. Rep. 6:34125-34125(2016).
RN   [9]
RP   GLYCOSYLATION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX   PubMed=29133832; DOI=10.1038/s41598-017-15676-z;
RA   Yeo K.S., Tan M.C., Lim Y.Y., Ea C.K.;
RT   "JMJD8 is a novel endoplasmic reticulum protein with a JmjC domain.";
RL   Sci. Rep. 7:15407-15407(2017).
CC   -!- FUNCTION: Functions as a positive regulator of TNF-induced NF-kappa-B
CC       signaling (PubMed:27671354). Regulates angiogenesis and cellular
CC       metabolism through interaction with PKM (PubMed:27199445).
CC       {ECO:0000269|PubMed:27199445, ECO:0000269|PubMed:27671354}.
CC   -!- SUBUNIT: Oligomer (PubMed:29133832). Dimer (PubMed:29133832). Interacts
CC       with PKM; regulates angiogenesis and metabolism (PubMed:27199445).
CC       {ECO:0000269|PubMed:27199445, ECO:0000269|PubMed:29133832}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen
CC       {ECO:0000269|PubMed:29133832}. Cytoplasm {ECO:0000269|PubMed:27199445}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q96S16-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q96S16-2; Sequence=VSP_034849;
CC   -!- PTM: N-glycosylated. {ECO:0000269|PubMed:29133832}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH73785.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAI37101.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAI37102.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAK61243.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=EAW85754.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=EAW85755.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AF370420; AAQ15256.1; -; mRNA.
DR   EMBL; DQ074695; AAY82251.1; -; mRNA.
DR   EMBL; AE006464; AAK61243.1; ALT_INIT; Genomic_DNA.
DR   EMBL; Z92544; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471112; EAW85754.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CH471112; EAW85755.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BC073785; AAH73785.1; ALT_INIT; mRNA.
DR   EMBL; BC094850; AAH94850.1; -; mRNA.
DR   EMBL; BC137100; AAI37101.1; ALT_INIT; mRNA.
DR   EMBL; BC137101; AAI37102.1; ALT_INIT; mRNA.
DR   CCDS; CCDS45369.2; -. [Q96S16-1]
DR   CCDS; CCDS92074.1; -. [Q96S16-2]
DR   RefSeq; NP_001005920.2; NM_001005920.2. [Q96S16-1]
DR   RefSeq; NP_001310847.1; NM_001323918.1.
DR   RefSeq; NP_001310848.1; NM_001323919.1.
DR   RefSeq; NP_001310851.1; NM_001323922.1.
DR   AlphaFoldDB; Q96S16; -.
DR   SMR; Q96S16; -.
DR   BioGRID; 130831; 53.
DR   IntAct; Q96S16; 11.
DR   STRING; 9606.ENSP00000399475; -.
DR   GlyCosmos; Q96S16; 3 sites, No reported glycans.
DR   GlyGen; Q96S16; 5 sites, 1 O-linked glycan (2 sites).
DR   iPTMnet; Q96S16; -.
DR   PhosphoSitePlus; Q96S16; -.
DR   BioMuta; JMJD8; -.
DR   DMDM; 74717267; -.
DR   EPD; Q96S16; -.
DR   MassIVE; Q96S16; -.
DR   PaxDb; 9606-ENSP00000399475; -.
DR   PeptideAtlas; Q96S16; -.
DR   ProteomicsDB; 78054; -. [Q96S16-1]
DR   ProteomicsDB; 78055; -. [Q96S16-2]
DR   Pumba; Q96S16; -.
DR   Antibodypedia; 22813; 109 antibodies from 22 providers.
DR   DNASU; 339123; -.
DR   Ensembl; ENST00000562824.5; ENSP00000454358.1; ENSG00000161999.13. [Q96S16-2]
DR   Ensembl; ENST00000609261.6; ENSP00000477481.1; ENSG00000161999.13. [Q96S16-1]
DR   GeneID; 339123; -.
DR   KEGG; hsa:339123; -.
DR   MANE-Select; ENST00000609261.6; ENSP00000477481.1; NM_001005920.4; NP_001005920.3.
DR   UCSC; uc002ciw.2; human. [Q96S16-1]
DR   AGR; HGNC:14148; -.
DR   CTD; 339123; -.
DR   DisGeNET; 339123; -.
DR   GeneCards; JMJD8; -.
DR   HGNC; HGNC:14148; JMJD8.
DR   HPA; ENSG00000161999; Low tissue specificity.
DR   MalaCards; JMJD8; -.
DR   neXtProt; NX_Q96S16; -.
DR   OpenTargets; ENSG00000161999; -.
DR   PharmGKB; PA162392531; -.
DR   VEuPathDB; HostDB:ENSG00000161999; -.
DR   eggNOG; KOG2131; Eukaryota.
DR   GeneTree; ENSGT00390000015438; -.
DR   InParanoid; Q96S16; -.
DR   OMA; KEPHFHP; -.
DR   OrthoDB; 120564at2759; -.
DR   PhylomeDB; Q96S16; -.
DR   TreeFam; TF313408; -.
DR   PathwayCommons; Q96S16; -.
DR   SignaLink; Q96S16; -.
DR   BioGRID-ORCS; 339123; 31 hits in 1158 CRISPR screens.
DR   ChiTaRS; JMJD8; human.
DR   GenomeRNAi; 339123; -.
DR   Pharos; Q96S16; Tbio.
DR   PRO; PR:Q96S16; -.
DR   Proteomes; UP000005640; Chromosome 16.
DR   RNAct; Q96S16; Protein.
DR   Bgee; ENSG00000161999; Expressed in apex of heart and 161 other cell types or tissues.
DR   ExpressionAtlas; Q96S16; baseline and differential.
DR   Genevisible; Q96S16; HS.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0043123; P:positive regulation of canonical NF-kappaB signal transduction; IMP:UniProtKB.
DR   GO; GO:1903672; P:positive regulation of sprouting angiogenesis; IMP:UniProtKB.
DR   GO; GO:0006110; P:regulation of glycolytic process; IMP:UniProtKB.
DR   GO; GO:1903302; P:regulation of pyruvate kinase activity; IMP:UniProtKB.
DR   Gene3D; 2.60.120.650; Cupin; 1.
DR   InterPro; IPR041667; Cupin_8.
DR   InterPro; IPR003347; JmjC_dom.
DR   PANTHER; PTHR12480; ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD; 1.
DR   PANTHER; PTHR12480:SF35; JMJC DOMAIN-CONTAINING PROTEIN 8; 1.
DR   Pfam; PF13621; Cupin_8; 1.
DR   SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR   PROSITE; PS51184; JMJC; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Endoplasmic reticulum; Glycoprotein;
KW   Reference proteome; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..264
FT                   /note="JmjC domain-containing protein 8"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000344531"
FT   DOMAIN          131..264
FT                   /note="JmjC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00538"
FT   CARBOHYD        130
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        140
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        209
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   VAR_SEQ         30..59
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_034849"
FT   CONFLICT        158
FT                   /note="L -> P (in Ref. 2; AAY82251)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        207
FT                   /note="H -> R (in Ref. 2; AAY82251)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   264 AA;  29509 MW;  764DAF7EE2977FBC CRC64;
     MAPASRLLAL WALAAVALPG SGAEGDGGWR PGGPGAVAEE ERCTVERRAD LTYAEFVQQY
     AFVRPVILQG LTDNSRFRAL CSRDRLLASF GDRVVRLSTA NTYSYHKVDL PFQEYVEQLL
     HPQDPTSLGN DTLYFFGDNN FTEWASLFRH YSPPPFGLLG TAPAYSFGIA GAGSGVPFHW
     HGPGYSEVIY GRKRWFLYPP EKTPEFHPNK TTLAWLRDTY PALPPSARPL ECTIRAGEVL
     YFPDRWWHAT LNLDTSVFIS TFLG
//