ID   HARB1_HUMAN             Reviewed;         349 AA.
AC   Q96MB7; D3DQP9;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   13-SEP-2023, entry version 140.
DE   RecName: Full=Putative nuclease HARBI1;
DE            EC=3.1.-.-;
DE   AltName: Full=Harbinger transposase-derived nuclease;
GN   Name=HARBI1; Synonyms=C11orf77;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pancreas;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   FUNCTION, COFACTOR, AND TISSUE SPECIFICITY.
RX   PubMed=15169610; DOI=10.1089/104454904323090949;
RA   Kapitonov V.V., Jurka J.;
RT   "Harbinger transposons and an ancient HARBI1 gene derived from a
RT   transposase.";
RL   DNA Cell Biol. 23:311-324(2004).
RN   [5]
RP   FUNCTION, INTERACTION WITH NAIF1, AND SUBCELLULAR LOCATION.
RX   PubMed=18339812; DOI=10.1073/pnas.0707746105;
RA   Sinzelle L., Kapitonov V.V., Grzela D.P., Jursch T., Jurka J., Izsvak Z.,
RA   Ivics Z.;
RT   "Transposition of a reconstructed Harbinger element in human cells and
RT   functional homology with two transposon-derived cellular genes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:4715-4720(2008).
CC   -!- FUNCTION: Transposase-derived protein that may have nuclease activity
CC       (Potential). Does not have transposase activity.
CC       {ECO:0000269|PubMed:15169610, ECO:0000269|PubMed:18339812,
CC       ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000305};
CC   -!- SUBUNIT: Interacts with NAIF1. {ECO:0000269|PubMed:18339812}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18339812}. Cytoplasm
CC       {ECO:0000269|PubMed:18339812}. Note=Interaction with NAIF1 promotes
CC       translocation to the nucleus.
CC   -!- TISSUE SPECIFICITY: Detected in brain, eye, nerve tissue, kidney and
CC       lung. {ECO:0000269|PubMed:15169610}.
CC   -!- SIMILARITY: Belongs to the HARBI1 family. {ECO:0000305}.
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DR   EMBL; AK057237; BAB71391.1; -; mRNA.
DR   EMBL; CH471064; EAW67992.1; -; Genomic_DNA.
DR   EMBL; CH471064; EAW67993.1; -; Genomic_DNA.
DR   EMBL; CH471064; EAW67994.1; -; Genomic_DNA.
DR   EMBL; BC036925; AAH36925.1; -; mRNA.
DR   CCDS; CCDS7920.1; -.
DR   RefSeq; NP_776172.1; NM_173811.3.
DR   RefSeq; XP_016873093.1; XM_017017604.1.
DR   RefSeq; XP_016873094.1; XM_017017605.1.
DR   RefSeq; XP_016873095.1; XM_017017606.1.
DR   AlphaFoldDB; Q96MB7; -.
DR   SMR; Q96MB7; -.
DR   BioGRID; 129511; 1.
DR   IntAct; Q96MB7; 1.
DR   STRING; 9606.ENSP00000317743; -.
DR   iPTMnet; Q96MB7; -.
DR   PhosphoSitePlus; Q96MB7; -.
DR   BioMuta; HARBI1; -.
DR   DMDM; 74732341; -.
DR   jPOST; Q96MB7; -.
DR   MassIVE; Q96MB7; -.
DR   MaxQB; Q96MB7; -.
DR   PaxDb; Q96MB7; -.
DR   PeptideAtlas; Q96MB7; -.
DR   ProteomicsDB; 77328; -.
DR   Antibodypedia; 42871; 115 antibodies from 19 providers.
DR   DNASU; 283254; -.
DR   Ensembl; ENST00000326737.3; ENSP00000317743.3; ENSG00000180423.4.
DR   GeneID; 283254; -.
DR   KEGG; hsa:283254; -.
DR   MANE-Select; ENST00000326737.3; ENSP00000317743.3; NM_173811.4; NP_776172.1.
DR   UCSC; uc001ncy.4; human.
DR   AGR; HGNC:26522; -.
DR   CTD; 283254; -.
DR   GeneCards; HARBI1; -.
DR   HGNC; HGNC:26522; HARBI1.
DR   HPA; ENSG00000180423; Low tissue specificity.
DR   MIM; 615086; gene.
DR   neXtProt; NX_Q96MB7; -.
DR   OpenTargets; ENSG00000180423; -.
DR   PharmGKB; PA162390577; -.
DR   VEuPathDB; HostDB:ENSG00000180423; -.
DR   eggNOG; KOG4585; Eukaryota.
DR   GeneTree; ENSGT00940000154348; -.
DR   HOGENOM; CLU_018552_13_0_1; -.
DR   InParanoid; Q96MB7; -.
DR   OMA; AHSATHN; -.
DR   OrthoDB; 5354790at2759; -.
DR   PhylomeDB; Q96MB7; -.
DR   TreeFam; TF327972; -.
DR   PathwayCommons; Q96MB7; -.
DR   SignaLink; Q96MB7; -.
DR   BioGRID-ORCS; 283254; 15 hits in 1161 CRISPR screens.
DR   ChiTaRS; HARBI1; human.
DR   GenomeRNAi; 283254; -.
DR   Pharos; Q96MB7; Tbio.
DR   PRO; PR:Q96MB7; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   RNAct; Q96MB7; Protein.
DR   Bgee; ENSG00000180423; Expressed in sperm and 114 other tissues.
DR   ExpressionAtlas; Q96MB7; baseline and differential.
DR   Genevisible; Q96MB7; HS.
DR   GO; GO:0034451; C:centriolar satellite; IDA:HPA.
DR   GO; GO:0005829; C:cytosol; IDA:HPA.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR026103; HARBI1_animal.
DR   InterPro; IPR027806; HARBI1_dom.
DR   PANTHER; PTHR22930:SF253; NUCLEASE HARBI1-RELATED; 1.
DR   PANTHER; PTHR22930; UNCHARACTERIZED; 1.
DR   Pfam; PF13359; DDE_Tnp_4; 1.
DR   PRINTS; PR02086; PUTNUCHARBI1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Hydrolase; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..349
FT                   /note="Putative nuclease HARBI1"
FT                   /id="PRO_0000263614"
FT   DOMAIN          148..300
FT                   /note="DDE Tnp4"
FT                   /evidence="ECO:0000255"
FT   BINDING         149
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         199
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         225
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         261
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   349 AA;  39146 MW;  C424FDE6825A69F2 CRC64;
     MAIPITVLDC DLLLYGRGHR TLDRFKLDDV TDEYLMSMYG FPRQFIYYLV ELLGANLSRP
     TQRSRAISPE TQVLAALGFY TSGSFQTRMG DAIGISQASM SRCVANVTEA LVERASQFIR
     FPADEASIQA LKDEFYGLAG MPGVMGVVDC IHVAIKAPNA EDLSYVNRKG LHSLNCLMVC
     DIRGTLMTVE TNWPGSLQDC AVLQQSSLSS QFEAGMHKDS WLLGDSSFFL RTWLMTPLHI
     PETPAEYRYN MAHSATHSVI EKTFRTLCSR FRCLDGSKGA LQYSPEKSSH IILACCVLHN
     ISLEHGMDVW SSPMTGPMEQ PPEEEYEHME SLDLEADRIR QELMLTHFS
//