ID   VSIG2_HUMAN             Reviewed;         327 AA.
AC   Q96IQ7; O95791; Q9NX42;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   02-DEC-2020, entry version 147.
DE   RecName: Full=V-set and immunoglobulin domain-containing protein 2;
DE   AltName: Full=Cortical thymocyte-like protein;
DE            Short=CT-like protein;
DE   Flags: Precursor;
GN   Name=VSIG2; Synonyms=CTH, CTXL; ORFNames=UNQ2770/PRO7154;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX   PubMed=9862345;
RX   DOI=10.1002/(sici)1521-4141(199812)28:12<4094::aid-immu4094>3.0.co;2-2;
RA   Chretien I., Marcuz A., Courtet M., Katevuo K., Vainio O., Heath J.K.,
RA   White S.J., Du Pasquier L.;
RT   "CTX, a Xenopus thymocyte receptor, defines a molecular family conserved
RT   throughout vertebrates.";
RL   Eur. J. Immunol. 28:4094-4104(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA   Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA   Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA   Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA   Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA   Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT   identify novel human secreted and transmembrane proteins: a bioinformatics
RT   assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PROTEIN SEQUENCE OF 24-38.
RX   PubMed=15340161; DOI=10.1110/ps.04682504;
RA   Zhang Z., Henzel W.J.;
RT   "Signal peptide prediction based on analysis of experimentally verified
RT   cleavage sites.";
RL   Protein Sci. 13:2819-2824(2004).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC       membrane protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q96IQ7-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q96IQ7-2; Sequence=VSP_014119;
CC   -!- TISSUE SPECIFICITY: Highly expressed in stomach, colon, prostate,
CC       trachea and thyroid glands and weakly in bladder and lung.
CC       {ECO:0000269|PubMed:9862345}.
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DR   EMBL; AF061022; AAD17522.1; -; Genomic_DNA.
DR   EMBL; AY358897; AAQ89256.1; -; mRNA.
DR   EMBL; AK000460; BAA91179.1; -; mRNA.
DR   EMBL; BC007313; AAH07313.1; -; mRNA.
DR   CCDS; CCDS8452.1; -. [Q96IQ7-1]
DR   CCDS; CCDS86258.1; -. [Q96IQ7-2]
DR   RefSeq; NP_001316849.1; NM_001329920.1. [Q96IQ7-2]
DR   RefSeq; NP_055127.2; NM_014312.4. [Q96IQ7-1]
DR   SMR; Q96IQ7; -.
DR   BioGRID; 117119; 30.
DR   IntAct; Q96IQ7; 25.
DR   STRING; 9606.ENSP00000318684; -.
DR   GlyGen; Q96IQ7; 2 sites.
DR   iPTMnet; Q96IQ7; -.
DR   PhosphoSitePlus; Q96IQ7; -.
DR   BioMuta; VSIG2; -.
DR   DMDM; 68053314; -.
DR   jPOST; Q96IQ7; -.
DR   MassIVE; Q96IQ7; -.
DR   PaxDb; Q96IQ7; -.
DR   PeptideAtlas; Q96IQ7; -.
DR   PRIDE; Q96IQ7; -.
DR   ProteomicsDB; 76843; -. [Q96IQ7-1]
DR   ProteomicsDB; 76844; -. [Q96IQ7-2]
DR   Antibodypedia; 32904; 232 antibodies.
DR   DNASU; 23584; -.
DR   Ensembl; ENST00000326621; ENSP00000318684; ENSG00000019102. [Q96IQ7-1]
DR   Ensembl; ENST00000403470; ENSP00000385013; ENSG00000019102. [Q96IQ7-2]
DR   GeneID; 23584; -.
DR   KEGG; hsa:23584; -.
DR   UCSC; uc001qas.4; human. [Q96IQ7-1]
DR   CTD; 23584; -.
DR   DisGeNET; 23584; -.
DR   EuPathDB; HostDB:ENSG00000019102.11; -.
DR   GeneCards; VSIG2; -.
DR   HGNC; HGNC:17149; VSIG2.
DR   HPA; ENSG00000019102; Tissue enhanced (gallbladder, stomach).
DR   MIM; 606011; gene.
DR   neXtProt; NX_Q96IQ7; -.
DR   OpenTargets; ENSG00000019102; -.
DR   PharmGKB; PA134937463; -.
DR   eggNOG; ENOG502RYI3; Eukaryota.
DR   GeneTree; ENSGT00940000161544; -.
DR   HOGENOM; CLU_040549_1_0_1; -.
DR   InParanoid; Q96IQ7; -.
DR   OMA; VFCLIRF; -.
DR   OrthoDB; 841952at2759; -.
DR   PhylomeDB; Q96IQ7; -.
DR   TreeFam; TF330875; -.
DR   PathwayCommons; Q96IQ7; -.
DR   BioGRID-ORCS; 23584; 2 hits in 840 CRISPR screens.
DR   ChiTaRS; VSIG2; human.
DR   GenomeRNAi; 23584; -.
DR   Pharos; Q96IQ7; Tbio.
DR   PRO; PR:Q96IQ7; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   RNAct; Q96IQ7; protein.
DR   Bgee; ENSG00000019102; Expressed in body of stomach and 166 other tissues.
DR   Genevisible; Q96IQ7; HS.
DR   GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR   GO; GO:0016020; C:membrane; TAS:ProtInc.
DR   Gene3D; 2.60.40.10; -; 2.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR003598; Ig_sub2.
DR   InterPro; IPR013106; Ig_V-set.
DR   InterPro; IPR042475; VSIG2.
DR   PANTHER; PTHR45046; PTHR45046; 1.
DR   Pfam; PF07686; V-set; 1.
DR   SMART; SM00409; IG; 2.
DR   SMART; SM00408; IGc2; 2.
DR   SMART; SM00406; IGv; 1.
DR   SUPFAM; SSF48726; SSF48726; 2.
DR   PROSITE; PS50835; IG_LIKE; 2.
PE   1: Evidence at protein level;
KW   Alternative splicing; Direct protein sequencing; Disulfide bond;
KW   Glycoprotein; Immunoglobulin domain; Membrane; Reference proteome; Repeat;
KW   Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:15340161"
FT   CHAIN           24..327
FT                   /note="V-set and immunoglobulin domain-containing protein
FT                   2"
FT                   /id="PRO_0000015004"
FT   TOPO_DOM        24..243
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        265..327
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          24..137
FT                   /note="Ig-like V-type"
FT   DOMAIN          144..233
FT                   /note="Ig-like C2-type"
FT   CARBOHYD        138
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        206
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        45..121
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   DISULFID        166..217
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   VAR_SEQ         285..327
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_014119"
FT   CONFLICT        3
FT                   /note="E -> D (in Ref. 1; AAD17522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        21
FT                   /note="Missing (in Ref. 1; AAD17522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        54
FT                   /note="S -> T (in Ref. 1; AAD17522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        223
FT                   /note="M -> L (in Ref. 1; AAD17522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        243
FT                   /note="A -> T (in Ref. 1; AAD17522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        311
FT                   /note="Missing (in Ref. 1; AAD17522)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   327 AA;  34348 MW;  CF395AC7EF951AC1 CRC64;
     MAELPGPFLC GALLGFLCLS GLAVEVKVPT EPLSTPLGKT AELTCTYSTS VGDSFALEWS
     FVQPGKPISE SHPILYFTNG HLYPTGSKSK RVSLLQNPPT VGVATLKLTD VHPSDTGTYL
     CQVNNPPDFY TNGLGLINLT VLVPPSNPLC SQSGQTSVGG STALRCSSSE GAPKPVYNWV
     RLGTFPTPSP GSMVQDEVSG QLILTNLSLT SSGTYRCVAT NQMGSASCEL TLSVTEPSQG
     RVAGALIGVL LGVLLLSVAA FCLVRFQKER GKKPKETYGG SDLREDAIAP GISEHTCMRA
     DSSKGFLERP SSASTVTTTK SKLPMVV
//