ID CALL4_HUMAN Reviewed; 196 AA. AC Q96GE6; B4DL15; F8W6Y4; Q6MZY3; Q6N048; Q9H286; DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot. DT 14-OCT-2008, sequence version 3. DT 27-MAR-2024, entry version 158. DE RecName: Full=Calmodulin-like protein 4 {ECO:0000305}; DE AltName: Full=Serologically defined breast cancer antigen NY-BR-20; GN Name=CALML4 {ECO:0000312|HGNC:HGNC:18445}; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY. RC TISSUE=Mammary gland; RX PubMed=12747765; RA Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O., RA Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J.; RT "Humoral immunity to human breast cancer: antigen definition and RT quantitative analysis of mRNA expression."; RL Cancer Immun. 1:4-4(2001). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC TISSUE=Placenta; RA Li W.B., Gruber C., Jessee J., Polayes D.; RT "Full-length cDNA libraries and normalization."; RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5). RC TISSUE=Prostate, and Uterine endothelium; RX PubMed=17974005; DOI=10.1186/1471-2164-8-399; RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., RA Wiemann S., Schupp I.; RT "The full-ORF clone resource of the German cDNA consortium."; RL BMC Genomics 8:399-399(2007). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=16572171; DOI=10.1038/nature04601; RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S., RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.; RT "Analysis of the DNA sequence and duplication history of human chromosome RT 15."; RL Nature 440:671-675(2006). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., RA Hunkapiller M.W., Myers E.W., Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC TISSUE=Colon; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-196 (ISOFORM 1). RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., RA Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [8] RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH MYO7B RP AND MYO7A, AND ALTERNATIVE SPLICING. RX PubMed=32209652; DOI=10.1074/jbc.ra120.012820; RA Choi M.S., Graves M.J., Matoo S., Storad Z.A., El Sheikh Idris R.A., RA Weck M.L., Smith Z.B., Tyska M.J., Crawley S.W.; RT "The small EF-hand protein CALML4 functions as a critical myosin light RT chain within the intermicrovillar adhesion complex."; RL J. Biol. Chem. 295:9281-9296(2020). CC -!- FUNCTION: As part of the intermicrovillar adhesion complex/IMAC plays a CC role in epithelial brush border differentiation, controlling microvilli CC organization and length. Acts as a light chain for MYO7B and is CC required for efficient targeting of the IMAC to the tips of border CC brush microvilli. {ECO:0000269|PubMed:32209652}. CC -!- SUBUNIT: Interacts with MYO7B; the interaction mediates the association CC of CALML4 with the IMAC/intermicrovillar adhesion complex CC (PubMed:32209652). Interacts with MYO7A (PubMed:32209652). CC {ECO:0000269|PubMed:32209652}. CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Cell projection, microvillus CC {ECO:0000269|PubMed:32209652}. Note=Enriched at the distal tips of CC enterocyte microvilli. {ECO:0000269|PubMed:32209652}. CC -!- SUBCELLULAR LOCATION: [Isoform 6]: Cell projection, microvillus CC {ECO:0000269|PubMed:32209652}. Note=Enriched at the distal tips of CC enterocyte microvilli. {ECO:0000269|PubMed:32209652}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=6; CC Name=1; Synonyms=Long {ECO:0000303|PubMed:32209652}; CC IsoId=Q96GE6-1; Sequence=Displayed; CC Name=2; CC IsoId=Q96GE6-2; Sequence=VSP_030436; CC Name=3; CC IsoId=Q96GE6-3; Sequence=VSP_030435; CC Name=4; CC IsoId=Q96GE6-4; Sequence=VSP_047150; CC Name=5; CC IsoId=Q96GE6-5; Sequence=VSP_055513, VSP_055514; CC Name=6; Synonyms=Short {ECO:0000303|PubMed:32209652}; CC IsoId=Q96GE6-6; Sequence=VSP_062262; CC -!- TISSUE SPECIFICITY: [Isoform 1]: Expressed in the intestinal tract. CC {ECO:0000269|PubMed:32209652}. CC -!- TISSUE SPECIFICITY: [Isoform 6]: Dominant transcript in the intestinal CC tract. {ECO:0000269|PubMed:32209652}. CC -!- SIMILARITY: Belongs to the calmodulin family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAG48255.1; Type=Frameshift; Evidence={ECO:0000305}; CC Sequence=BAG59377.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF308287; AAG48255.1; ALT_SEQ; mRNA. DR EMBL; CR598967; -; NOT_ANNOTATED_CDS; mRNA. DR EMBL; BX640702; CAE45822.1; -; mRNA. DR EMBL; BX640817; CAE45894.1; -; mRNA. DR EMBL; AC107871; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471082; EAW77810.1; -; Genomic_DNA. DR EMBL; BC009516; -; NOT_ANNOTATED_CDS; mRNA. DR EMBL; AK296804; BAG59377.1; ALT_INIT; mRNA. DR CCDS; CCDS66808.1; -. [Q96GE6-2] DR CCDS; CCDS92031.1; -. [Q96GE6-3] DR RefSeq; NP_001026903.2; NM_001031733.2. DR RefSeq; NP_001273623.1; NM_001286694.1. [Q96GE6-2] DR RefSeq; NP_001273624.1; NM_001286695.1. [Q96GE6-3] DR RefSeq; NP_219501.2; NM_033429.2. DR AlphaFoldDB; Q96GE6; -. DR SMR; Q96GE6; -. DR BioGRID; 124886; 4. DR IntAct; Q96GE6; 1. DR STRING; 9606.ENSP00000419081; -. DR iPTMnet; Q96GE6; -. DR PhosphoSitePlus; Q96GE6; -. DR BioMuta; CALML4; -. DR DMDM; 209572745; -. DR EPD; Q96GE6; -. DR jPOST; Q96GE6; -. DR MassIVE; Q96GE6; -. DR MaxQB; Q96GE6; -. DR PaxDb; 9606-ENSP00000419081; -. DR PeptideAtlas; Q96GE6; -. DR ProteomicsDB; 29855; -. DR ProteomicsDB; 76627; -. [Q96GE6-1] DR ProteomicsDB; 76628; -. [Q96GE6-2] DR ProteomicsDB; 76629; -. [Q96GE6-3] DR Antibodypedia; 42938; 137 antibodies from 21 providers. DR DNASU; 91860; -. DR Ensembl; ENST00000395463.3; ENSP00000435285.2; ENSG00000129007.16. [Q96GE6-2] DR Ensembl; ENST00000540479.6; ENSP00000438177.2; ENSG00000129007.16. [Q96GE6-3] DR GeneID; 91860; -. DR KEGG; hsa:91860; -. DR UCSC; uc002arb.4; human. [Q96GE6-1] DR AGR; HGNC:18445; -. DR CTD; 91860; -. DR GeneCards; CALML4; -. DR HGNC; HGNC:18445; CALML4. DR HPA; ENSG00000129007; Tissue enhanced (choroid plexus, intestine). DR MIM; 620520; gene. DR neXtProt; NX_Q96GE6; -. DR OpenTargets; ENSG00000129007; -. DR PharmGKB; PA134903345; -. DR VEuPathDB; HostDB:ENSG00000129007; -. DR eggNOG; KOG0027; Eukaryota. DR GeneTree; ENSGT00940000157859; -. DR HOGENOM; CLU_2995949_0_0_1; -. DR InParanoid; Q96GE6; -. DR OMA; KFLTQAQ; -. DR OrthoDB; 3012240at2759; -. DR PhylomeDB; Q96GE6; -. DR TreeFam; TF300912; -. DR PathwayCommons; Q96GE6; -. DR BioGRID-ORCS; 91860; 7 hits in 1152 CRISPR screens. DR ChiTaRS; CALML4; human. DR GenomeRNAi; 91860; -. DR Pharos; Q96GE6; Tdark. DR PRO; PR:Q96GE6; -. DR Proteomes; UP000005640; Chromosome 15. DR RNAct; Q96GE6; Protein. DR Bgee; ENSG00000129007; Expressed in mucosa of transverse colon and 95 other cell types or tissues. DR ExpressionAtlas; Q96GE6; baseline and differential. DR Genevisible; Q96GE6; HS. DR GO; GO:0005902; C:microvillus; IDA:UniProtKB. DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central. DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central. DR GO; GO:0032028; F:myosin head/neck binding; IPI:UniProtKB. DR GO; GO:1904970; P:brush border assembly; IDA:UniProtKB. DR GO; GO:0006936; P:muscle contraction; IEA:UniProt. DR CDD; cd00051; EFh; 2. DR Gene3D; 1.10.238.10; EF-hand; 1. DR InterPro; IPR011992; EF-hand-dom_pair. DR InterPro; IPR002048; EF_hand_dom. DR PANTHER; PTHR23048:SF52; CALMODULIN-LIKE PROTEIN 4; 1. DR PANTHER; PTHR23048; MYOSIN LIGHT CHAIN 1, 3; 1. DR Pfam; PF13499; EF-hand_7; 1. DR SMART; SM00054; EFh; 4. DR SUPFAM; SSF47473; EF-hand; 1. DR PROSITE; PS50222; EF_HAND_2; 4. PE 1: Evidence at protein level; KW Alternative splicing; Cell projection; Reference proteome; Repeat. FT CHAIN 1..196 FT /note="Calmodulin-like protein 4" FT /id="PRO_0000314933" FT DOMAIN 51..86 FT /note="EF-hand 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" FT DOMAIN 87..122 FT /note="EF-hand 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" FT DOMAIN 124..159 FT /note="EF-hand 3" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" FT DOMAIN 160..195 FT /note="EF-hand 4" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" FT REGION 1..43 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 29..43 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT VAR_SEQ 1..115 FT /note="Missing (in isoform 3)" FT /evidence="ECO:0000303|PubMed:17974005" FT /id="VSP_030435" FT VAR_SEQ 1..76 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.2" FT /id="VSP_030436" FT VAR_SEQ 1..43 FT /note="Missing (in isoform 6)" FT /id="VSP_062262" FT VAR_SEQ 45..59 FT /note="AKFLSQDQINEYKEC -> WMISSGKQISNPMAK (in isoform 5)" FT /evidence="ECO:0000303|PubMed:17974005" FT /id="VSP_055513" FT VAR_SEQ 55..101 FT /note="Missing (in isoform 4)" FT /evidence="ECO:0000305" FT /id="VSP_047150" FT VAR_SEQ 60..196 FT /note="Missing (in isoform 5)" FT /evidence="ECO:0000303|PubMed:17974005" FT /id="VSP_055514" FT VARIANT 28 FT /note="R -> C (in dbSNP:rs3803381)" FT /id="VAR_048586" FT VARIANT 154 FT /note="T -> K (in dbSNP:rs2280217)" FT /id="VAR_048587" FT CONFLICT 40 FT /note="R -> G (in Ref. 1; AAG48255)" FT /evidence="ECO:0000305" FT CONFLICT 60 FT /note="F -> S (in Ref. 1; AAG48255)" FT /evidence="ECO:0000305" FT CONFLICT 76 FT /note="L -> F (in Ref. 1; AAG48255)" FT /evidence="ECO:0000305" SQ SEQUENCE 196 AA; 21883 MW; 547C558AC7EDFABD CRC64; MAAEHLLPGP PPSLADFRLE AGGKGTERGS GSSKPTGSSR GPRMAKFLSQ DQINEYKECF SLYDKQQRGK IKATDLMVAM RCLGASPTPG EVQRHLQTHG IDGNGELDFS TFLTIMHMQI KQEDPKKEIL LAMLMVDKEK KGYVMASDLR SKLTSLGEKL THKEVDDLFR EADIEPNGKV KYDEFIHKIT LPGRDY //