ID   CALL4_HUMAN             Reviewed;         196 AA.
AC   Q96GE6; B4DL15; F8W6Y4; Q6MZY3; Q6N048; Q9H286;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 3.
DT   12-OCT-2022, entry version 150.
DE   RecName: Full=Calmodulin-like protein 4;
DE   AltName: Full=Serologically defined breast cancer antigen NY-BR-20;
GN   Name=CALML4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC   TISSUE=Mammary gland;
RX   PubMed=12747765;
RA   Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O.,
RA   Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J.;
RT   "Humoral immunity to human breast cancer: antigen definition and
RT   quantitative analysis of mRNA expression.";
RL   Cancer Immun. 1:4-4(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Placenta;
RA   Li W.B., Gruber C., Jessee J., Polayes D.;
RT   "Full-length cDNA libraries and normalization.";
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5).
RC   TISSUE=Prostate, and Uterine endothelium;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16572171; DOI=10.1038/nature04601;
RA   Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA   Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA   FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA   Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA   Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA   DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA   Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA   Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA   Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA   O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA   Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA   Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT   "Analysis of the DNA sequence and duplication history of human chromosome
RT   15.";
RL   Nature 440:671-675(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-196 (ISOFORM 1).
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=5;
CC       Name=1;
CC         IsoId=Q96GE6-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q96GE6-2; Sequence=VSP_030436;
CC       Name=3;
CC         IsoId=Q96GE6-3; Sequence=VSP_030435;
CC       Name=4;
CC         IsoId=Q96GE6-4; Sequence=VSP_047150;
CC       Name=5;
CC         IsoId=Q96GE6-5; Sequence=VSP_055513, VSP_055514;
CC   -!- TISSUE SPECIFICITY: Expressed in breast cancer cell lines.
CC       {ECO:0000269|PubMed:12747765}.
CC   -!- SIMILARITY: Belongs to the calmodulin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG48255.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAG59377.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF308287; AAG48255.1; ALT_SEQ; mRNA.
DR   EMBL; CR598967; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BX640702; CAE45822.1; -; mRNA.
DR   EMBL; BX640817; CAE45894.1; -; mRNA.
DR   EMBL; AC107871; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471082; EAW77810.1; -; Genomic_DNA.
DR   EMBL; BC009516; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AK296804; BAG59377.1; ALT_INIT; mRNA.
DR   CCDS; CCDS66808.1; -. [Q96GE6-2]
DR   RefSeq; NP_001026903.2; NM_001031733.2.
DR   RefSeq; NP_001273623.1; NM_001286694.1. [Q96GE6-2]
DR   RefSeq; NP_001273624.1; NM_001286695.1. [Q96GE6-3]
DR   RefSeq; NP_219501.2; NM_033429.2.
DR   AlphaFoldDB; Q96GE6; -.
DR   SMR; Q96GE6; -.
DR   BioGRID; 124886; 2.
DR   IntAct; Q96GE6; 1.
DR   STRING; 9606.ENSP00000419081; -.
DR   iPTMnet; Q96GE6; -.
DR   PhosphoSitePlus; Q96GE6; -.
DR   BioMuta; CALML4; -.
DR   DMDM; 209572745; -.
DR   EPD; Q96GE6; -.
DR   jPOST; Q96GE6; -.
DR   MassIVE; Q96GE6; -.
DR   MaxQB; Q96GE6; -.
DR   PaxDb; Q96GE6; -.
DR   PeptideAtlas; Q96GE6; -.
DR   PRIDE; Q96GE6; -.
DR   ProteomicsDB; 29855; -.
DR   ProteomicsDB; 76627; -. [Q96GE6-1]
DR   ProteomicsDB; 76628; -. [Q96GE6-2]
DR   ProteomicsDB; 76629; -. [Q96GE6-3]
DR   Antibodypedia; 42938; 132 antibodies from 21 providers.
DR   DNASU; 91860; -.
DR   Ensembl; ENST00000395463.3; ENSP00000435285.2; ENSG00000129007.16. [Q96GE6-2]
DR   Ensembl; ENST00000540479.6; ENSP00000438177.2; ENSG00000129007.16. [Q96GE6-3]
DR   GeneID; 91860; -.
DR   KEGG; hsa:91860; -.
DR   UCSC; uc002arb.4; human. [Q96GE6-1]
DR   CTD; 91860; -.
DR   GeneCards; CALML4; -.
DR   HGNC; HGNC:18445; CALML4.
DR   HPA; ENSG00000129007; Tissue enhanced (intestine).
DR   neXtProt; NX_Q96GE6; -.
DR   OpenTargets; ENSG00000129007; -.
DR   PharmGKB; PA134903345; -.
DR   VEuPathDB; HostDB:ENSG00000129007; -.
DR   eggNOG; KOG0027; Eukaryota.
DR   GeneTree; ENSGT00940000157859; -.
DR   HOGENOM; CLU_2995949_0_0_1; -.
DR   InParanoid; Q96GE6; -.
DR   OMA; RMTDKQN; -.
DR   OrthoDB; 1386217at2759; -.
DR   PhylomeDB; Q96GE6; -.
DR   TreeFam; TF300912; -.
DR   PathwayCommons; Q96GE6; -.
DR   BioGRID-ORCS; 91860; 7 hits in 1074 CRISPR screens.
DR   ChiTaRS; CALML4; human.
DR   GenomeRNAi; 91860; -.
DR   Pharos; Q96GE6; Tdark.
DR   PRO; PR:Q96GE6; -.
DR   Proteomes; UP000005640; Chromosome 15.
DR   RNAct; Q96GE6; protein.
DR   Bgee; ENSG00000129007; Expressed in mucosa of transverse colon and 93 other tissues.
DR   ExpressionAtlas; Q96GE6; baseline and differential.
DR   Genevisible; Q96GE6; HS.
DR   GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR   GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR   CDD; cd00051; EFh; 2.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13499; EF-hand_7; 2.
DR   SMART; SM00054; EFh; 4.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS50222; EF_HAND_2; 4.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Reference proteome; Repeat.
FT   CHAIN           1..196
FT                   /note="Calmodulin-like protein 4"
FT                   /id="PRO_0000314933"
FT   DOMAIN          51..86
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          87..122
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          124..159
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          160..195
FT                   /note="EF-hand 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        29..43
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..115
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:17974005"
FT                   /id="VSP_030435"
FT   VAR_SEQ         1..76
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.2"
FT                   /id="VSP_030436"
FT   VAR_SEQ         45..59
FT                   /note="AKFLSQDQINEYKEC -> WMISSGKQISNPMAK (in isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:17974005"
FT                   /id="VSP_055513"
FT   VAR_SEQ         55..101
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_047150"
FT   VAR_SEQ         60..196
FT                   /note="Missing (in isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:17974005"
FT                   /id="VSP_055514"
FT   VARIANT         28
FT                   /note="R -> C (in dbSNP:rs3803381)"
FT                   /id="VAR_048586"
FT   VARIANT         154
FT                   /note="T -> K (in dbSNP:rs2280217)"
FT                   /id="VAR_048587"
FT   CONFLICT        40
FT                   /note="R -> G (in Ref. 1; AAG48255)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        60
FT                   /note="F -> S (in Ref. 1; AAG48255)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        76
FT                   /note="L -> F (in Ref. 1; AAG48255)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   196 AA;  21883 MW;  547C558AC7EDFABD CRC64;
     MAAEHLLPGP PPSLADFRLE AGGKGTERGS GSSKPTGSSR GPRMAKFLSQ DQINEYKECF
     SLYDKQQRGK IKATDLMVAM RCLGASPTPG EVQRHLQTHG IDGNGELDFS TFLTIMHMQI
     KQEDPKKEIL LAMLMVDKEK KGYVMASDLR SKLTSLGEKL THKEVDDLFR EADIEPNGKV
     KYDEFIHKIT LPGRDY
//