ID   GCP3_HUMAN              Reviewed;         907 AA.
AC   Q96CW5; O43631; O60852; O60853; Q7Z4K1; Q96I79;
DT   06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   06-JUN-2002, sequence version 2.
DT   15-MAY-2007, entry version 44.
DE   Gamma-tubulin complex component 3 (GCP-3) (Spindle pole body protein
DE   Spc98 homolog) (hSpc98) (hGCP3) (h104p).
GN   Name=TUBGCP3; Synonyms=GCP3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Cervix carcinoma;
RX   MEDLINE=98234402; PubMed=9566967; DOI=10.1083/jcb.141.3.663;
RA   Murphy S.M., Urbani L., Stearns T.;
RT   "The mammalian gamma-tubulin complex contains homologues of the yeast
RT   spindle pole body components spc97p and spc98p.";
RL   J. Cell Biol. 141:663-674(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND CHARACTERIZATION.
RX   MEDLINE=98234404; PubMed=9566969; DOI=10.1083/jcb.141.3.689;
RA   Tassin A.-M., Celati C., Moudjou M., Bornens M.;
RT   "Characterization of the human homologue of the yeast spc98p and its
RT   association with gamma-tubulin.";
RL   J. Cell Biol. 141:689-701(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT   vector.";
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057823; DOI=10.1038/nature02379;
RA   Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA   Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E.,
RA   Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.,
RA   Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R.,
RA   Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S.,
RA   Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA   Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M.,
RA   Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J.,
RA   Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E.,
RA   Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L.,
RA   Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J.,
RA   Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S.,
RA   Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J.,
RA   Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M.,
RA   King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A.,
RA   Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S.,
RA   Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA   Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I.,
RA   Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S.,
RA   Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A.,
RA   Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B.,
RA   Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L.,
RA   Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M.,
RA   Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.;
RT   "The DNA sequence and analysis of human chromosome 13.";
RL   Nature 428:522-528(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC   TISSUE=Eye, Muscle, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Gamma-tubulin complex is necessary for microtubule
CC       nucleation at the centrosome.
CC   -!- SUBUNIT: Gamma-tubulin complex is composed of gamma-tubulin, GCP2,
CC       GCP3, GCP4, GCP5 and GCP6.
CC   -!- SUBCELLULAR LOCATION: Centrosome.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC         Comment=Experimental confirmation may be lacking for some
CC         isoforms;
CC       Name=1;
CC         IsoId=Q96CW5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q96CW5-2; Sequence=VSP_001620, VSP_001621;
CC       Name=3;
CC         IsoId=Q96CW5-3; Sequence=VSP_001622, VSP_001623;
CC   -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC   -!- SIMILARITY: Belongs to the GCP family.
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DR   EMBL; AF042378; AAC39727.1; -; mRNA.
DR   EMBL; AJ003061; CAA05832.1; -; mRNA.
DR   EMBL; AJ003062; CAA05833.1; -; mRNA.
DR   EMBL; BT009772; AAP88774.1; -; mRNA.
DR   EMBL; AL139384; CAI16950.1; -; Genomic_DNA.
DR   EMBL; AL160033; CAI16950.1; JOINED; Genomic_DNA.
DR   EMBL; AL160033; CAI14456.1; -; Genomic_DNA.
DR   EMBL; AL139384; CAI14456.1; JOINED; Genomic_DNA.
DR   EMBL; BC007763; AAH07763.1; -; mRNA.
DR   EMBL; BC013781; AAH13781.1; -; mRNA.
DR   EMBL; BC046634; AAH46634.1; -; mRNA.
DR   UniGene; Hs.224152; -.
DR   Ensembl; ENSG00000126216; Homo sapiens.
DR   H-InvDB; HIX0011466; -.
DR   HGNC; HGNC:18598; TUBGCP3.
DR   LinkHub; Q96CW5; -.
DR   ArrayExpress; Q96CW5; -.
DR   GermOnline; ENSG00000126216; Homo sapiens.
DR   RZPD-ProtExp; IOH25856; -.
DR   RZPD-ProtExp; IOH6459; -.
DR   RZPD-ProtExp; IOH9733; -.
DR   GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR   GO; GO:0005827; C:polar microtubule; IDA:UniProtKB.
DR   GO; GO:0043015; F:gamma-tubulin binding; IDA:UniProtKB.
DR   GO; GO:0005200; F:structural constituent of cytoskeleton; NAS:UniProtKB.
DR   GO; GO:0007020; P:microtubule nucleation; NAS:UniProtKB.
DR   InterPro; IPR007259; Spc97_Spc98.
DR   PANTHER; PTHR19302:SF14; PTHR19302:SF14; 2.
DR   PANTHER; PTHR19302; Spc97_Spc98; 2.
DR   Pfam; PF04130; Spc97_Spc98; 1.
KW   Alternative splicing; Microtubule.
FT   CHAIN         1    907       Gamma-tubulin complex component 3.
FT                                /FTId=PRO_0000078118.
FT   COMPBIAS    825    829       Poly-Glu.
FT   VAR_SEQ     391    434       RKGGELASAVHAYTKTGDPYMRSLVQHILSLVSHPVLSFLY
FT                                RWI -> PTRVFPTHVFPTRDFPTRDFPMHVFPTRVFPTRV
FT                                WHSLCFRTRL (in isoform 3).
FT                                /FTId=VSP_001622.
FT   VAR_SEQ     435    907       Missing (in isoform 3).
FT                                /FTId=VSP_001623.
FT   VAR_SEQ     817    824       GQWGVTAA -> VEMCLYCV (in isoform 2).
FT                                /FTId=VSP_001620.
FT   VAR_SEQ     825    907       Missing (in isoform 2).
FT                                /FTId=VSP_001621.
FT   CONFLICT    208    208       T -> S (in Ref. 2).
FT   CONFLICT    361    361       S -> I (in Ref. 2).
SQ   SEQUENCE   907 AA;  103571 MW;  70FE2FDB7C80344D CRC64;
     MATPDQKSPN VLLQNLCCRI LGRSEADVAQ QFQYAVRVIG SNFAPTVERD EFLVAEKIKK
     ELIRQRREAD AALFSELHRK LHSQGVLKNK WSILYLLLSL SEDPRRQPSK VSSYATLFAQ
     ALPRDAHSTP YYYARPQTLP LSYQDRSAQS AQSSGSVGSS GISSIGLCAL SGPAPAPQSL
     LPGQSNQAPG VGDCLRQQLG SRLAWTLTAN QPSSQATTSK GVPSAVSRNM TRSRREGDTG
     GTMEITEAAL VRDILYVFQG IDGKNIKMNN TENCYKVEGK ANLSRSLRDT AVRLSELGWL
     HNKIRRYTDQ RSLDRSFGLV GQSFCAALHQ ELREYYRLLS VLHSQLQLED DQGVNLGLES
     SLTLRRLLVW TYDPKIRLKT LAALVDHCQG RKGGELASAV HAYTKTGDPY MRSLVQHILS
     LVSHPVLSFL YRWIYDGELE DTYHEFFVAS DPTVKTDRLW HDKYTLRKSM IPSFMTMDQS
     RKVLLIGKSI NFLHQVCHDQ TPTTKMIAVT KSAESPQDAA DLFTDLENAF QGKIDAAYFE
     TSKYLLDVLN KKYSLLDHMQ AMRRYLLLGQ GDFIRHLMDL LKPELVRPAT TLYQHNLTGI
     LETAVRATNA QFDSPEILRR LDVRLLEVSP GDTGWDVFSL DYHVDGPIAT VFTRECMSHY
     LRVFNFLWRA KRMEYILTDI RKGHMCNAKL LRNMPEFSGV LHQCHILASE MVHFIHQMQY
     YITFEVLECS WDELWNKVQQ AQDLDHIIAA HEVFLDTIIS RCLLDSDSRA LLNQLRAVFD
     QIIELQNAQD AIYRAALEEL QRRLQFEEKK KQREIEGQWG VTAAEEEEEN KRIGEFKESI
     PKMCSQLRIL THFYQGIVQQ FLVLLTTSSD ESLRFLSFRL DFNEHYKARE PRLRVSLGTR
     GRRSSHT
//