ID GRM1A_HUMAN Reviewed; 724 AA. AC Q96CP6; A6NKY7; Q8NC77; Q9P1Z5; DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot. DT 16-MAY-2006, sequence version 2. DT 13-OCT-2009, entry version 45. DE RecName: Full=GRAM domain-containing protein 1A; GN Name=GRAMD1A; Synonyms=KIAA1533; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RX PubMed=16303743; DOI=10.1093/dnares/12.2.117; RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y., RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., RA Isogai T.; RT "Signal sequence and keyword trap in silico for selection of full- RT length human cDNAs encoding secretion or membrane proteins from oligo- RT capped cDNA libraries."; RL DNA Res. 12:117-126(2005). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15057824; DOI=10.1038/nature02399; RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., RA Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., RA Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., RA Caenepeel S., Carrano A.V., Caoile C., Chan Y.M., Christensen M., RA Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., RA Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., RA Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., RA Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., RA Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., RA Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., RA Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., RA Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., RA Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., RA Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., RA Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., RA Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., RA Rubin E.M., Lucas S.M.; RT "The DNA sequence and biology of human chromosome 19."; RL Nature 428:529-535(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC TISSUE=Brain; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 71-724 (ISOFORM 3). RC TISSUE=Brain; RX MEDLINE=20277482; PubMed=10819331; DOI=10.1093/dnares/7.2.143; RA Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.; RT "Prediction of the coding sequences of unidentified human genes. XVII. RT The complete sequences of 100 new cDNA clones from brain which code RT for large proteins in vitro."; RL DNA Res. 7:143-150(2000). RN [5] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-560, AND MASS RP SPECTROMETRY. RC TISSUE=Epithelium; RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026; RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., RA Mann M.; RT "Global, in vivo, and site-specific phosphorylation dynamics in RT signaling networks."; RL Cell 127:635-648(2006). RN [6] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-281, AND MASS RP SPECTROMETRY. RX PubMed=18220336; DOI=10.1021/pr0705441; RA Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., RA Yates J.R. III; RT "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for RT efficient phosphoproteomic analysis."; RL J. Proteome Res. 7:1346-1351(2008). RN [7] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263; SER-267; THR-277; RP SER-281 AND SER-550, AND MASS SPECTROMETRY. RX PubMed=18669648; DOI=10.1073/pnas.0805139105; RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., RA Elledge S.J., Gygi S.P.; RT "A quantitative atlas of mitotic phosphorylation."; RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein CC (Potential). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q96CP6-1; Sequence=Displayed; CC Name=2; CC IsoId=Q96CP6-2; Sequence=VSP_025465, VSP_025466; CC Note=No experimental confirmation available; CC Name=3; CC IsoId=Q96CP6-3; Sequence=VSP_025466; CC Note=No experimental confirmation available; CC -!- SIMILARITY: Contains 1 GRAM domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK074914; BAC11289.1; -; mRNA. DR EMBL; AC020907; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; BC014077; AAH14077.2; -; mRNA. DR EMBL; AB040966; BAA96057.1; -; mRNA. DR IPI; IPI00168347; -. DR IPI; IPI00180434; -. DR IPI; IPI00845289; -. DR RefSeq; NP_001129671.1; -. DR RefSeq; NP_065946.2; -. DR UniGene; Hs.515351; -. DR PhosphoSite; Q96CP6; -. DR Ensembl; ENST00000317991; ENSP00000322883; ENSG00000089351; Homo sapiens. DR Ensembl; ENST00000411896; ENSP00000410006; ENSG00000089351; Homo sapiens. DR Ensembl; ENST00000453966; ENSP00000393821; ENSG00000089351; Homo sapiens. DR GeneID; 57655; -. DR KEGG; hsa:57655; -. DR UCSC; uc002nxj.1; human. DR UCSC; uc002nxk.1; human. DR CTD; 57655; -. DR GeneCards; GC19P040174; -. DR HGNC; HGNC:29305; GRAMD1A. DR HPA; HPA008852; -. DR HPA; HPA012570; -. DR PharmGKB; PA134869487; -. DR HOGENOM; Q96CP6; -. DR HOVERGEN; Q96CP6; -. DR OMA; Q96CP6; LLDEMKF. DR NextBio; 64409; -. DR ArrayExpress; Q96CP6; -. DR Bgee; Q96CP6; -. DR CleanEx; HS_GRAMD1A; -. DR Genevestigator; Q96CP6; -. DR GO; GO:0005737; C:cytoplasm; IDA:HPA. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005886; C:plasma membrane; IDA:HPA. DR InterPro; IPR004182; GRAM. DR Pfam; PF02893; GRAM; 1. DR SMART; SM00568; GRAM; 1. PE 1: Evidence at protein level; KW Alternative splicing; Complete proteome; Membrane; Phosphoprotein; KW Transmembrane. FT CHAIN 1 724 GRAM domain-containing protein 1A. FT /FTId=PRO_0000287446. FT TRANSMEM 610 630 Potential. FT DOMAIN 91 158 GRAM. FT MOD_RES 263 263 Phosphoserine. FT MOD_RES 267 267 Phosphoserine. FT MOD_RES 277 277 Phosphothreonine. FT MOD_RES 281 281 Phosphoserine. FT MOD_RES 550 550 Phosphoserine. FT MOD_RES 560 560 Phosphoserine. FT VAR_SEQ 74 80 Missing (in isoform 2). FT /FTId=VSP_025465. FT VAR_SEQ 615 618 Missing (in isoform 2 and isoform 3). FT /FTId=VSP_025466. FT CONFLICT 439 439 S -> P (in Ref. 1; BAC11289). FT CONFLICT 477 477 H -> R (in Ref. 1; BAC11289). SQ SEQUENCE 724 AA; 80680 MW; 39D988FAD1327D14 CRC64; MFDTTPHSGR STPSSSPSLR KRLQLLPPSR PPPEPEPGTM VEKGSDSSSE KGGVPGTPST QSLGSRNFIR NSKKMQSWYS MLSPTYKQRN EDFRKLFSKL PEAERLIVDY SCALQREILL QGRLYLSENW ICFYSNIFRW ETTISIQLKE VTCLKKEKTA KLIPNAIQIC TESEKHFFTS FGARDRCFLL IFRLWQNALL EKTLSPRELW HLVHQCYGSE LGLTSEDEDY VSPLQLNGLG TPKEVGDVIA LSDITSSGAA DRSQEPSPVG SRRGHVTPNL SRASSDADHG AEEDKEEQVD SQPDASSSQT VTPVAEPPST EPTQPDGPTT LGPLDLLPSE ELLTDTSNSS SSTGEEADLA ALLPDLSGRL LINSVFHVGA ERLQQMLFSD SPFLQGFLQQ CKFTDVTLSP WSGDSKCHQR RVLTYTIPIS NPLGPKSASV VETQTLFRRG PQAGGCVVDS EVLTQGIPYQ DYFYTAHRYC ILGLARNKAR LRVSSEIRYR KQPWSLVKSL IEKNSWSGIE DYFHHLEREL AKAEKLSLEE GGKDARGLLS GLRRRKRPLS WRAHGDGPQH PDPDPCARAG IHTSGSLSSR FSEPSVDQGP GAGIPSALVL ISIVICVSLI ILIALNVLLF YRLWSLERTA HTFESWHSLA LAKGKFPQTA TEWAEILALQ KQFHSVEVHK WRQILRASVE LLDEMKFSLE KLHQGITVSD PPFDTQPRPD DSFS //