ID EAF2_HUMAN STANDARD; PRT; 260 AA. AC Q96CJ1; Q9NZ82; DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2001, sequence version 1. DT 16-MAY-2006, entry version 23. DE ELL-associated factor 2 (Testosterone-regulated apoptosis inducer and DE tumor suppressor protein). GN Name=EAF2; Synonyms=TRAITS; ORFNames=BM040; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN TRANSCRIPTION RP ACTIVATION, INTERACTION WITH ELL AND ELL2, SUBCELLULAR LOCATION, AND RP TISSUE SPECIFICITY. RC TISSUE=Bone marrow; RX MEDLINE=22496791; PubMed=12446457; DOI=10.1182/blood-2002-06-1664; RA Simone F., Luo R.T., Polak P.E., Kaberlein J.J., Thirman M.J.; RT "ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with RT alternative ELL binding properties."; RL Blood 101:2355-2362(2003). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN APOPTOSIS, RP INDUCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY. RX MEDLINE=22788499; PubMed=12907652; RA Xiao W., Zhang Q., Jiang F., Pins M., Kozlowski J.M., Wang Z.; RT "Suppression of prostate tumor growth by U19, a novel testosterone- RT regulated apoptosis inducer."; RL Cancer Res. 63:4698-4704(2003). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC TISSUE=Bone marrow; RX MEDLINE=20499367; PubMed=11042152; DOI=10.1101/gr.140200; RA Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., RA Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., RA Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.; RT "Cloning and functional analysis of cDNAs with open reading frames for RT 300 previously undefined genes expressed in CD34+ hematopoietic RT stem/progenitor cells."; RL Genome Res. 10:1546-1560(2000). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC TISSUE=B-cell; RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human RT and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [5] RP ROLE IN ELL-TRANSCRIPTION ACTIVATION. RX PubMed=16006523; DOI=10.1073/pnas.0503017102; RA Kong S.E., Banks C.A., Shilatifard A., Conaway J.W., Conaway R.C.; RT "ELL-associated factors 1 and 2 are positive regulators of RNA RT polymerase II elongation factor ELL."; RL Proc. Natl. Acad. Sci. U.S.A. 102:10094-10098(2005). CC -!- FUNCTION: Acts as a transcriptional transactivator of TCEA1 CC elongation activity (By similarity). Acts as a transcriptional CC transactivator of ELL and ELL2 elongation activities. Potent CC inducer of apoptosis in prostatic and non-prostatic cell lines. CC Inhibits prostate tumor growth in vivo. CC -!- SUBUNIT: Isoform 1 and isoform 2 interact with TCEA1 (By CC similarity). Interacts with ELL and ELL2. CC -!- SUBCELLULAR LOCATION: Nucleus; nucleoplasm; nuclear speckle. CC Colocalizes with ELL to nuclear speckles. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q96CJ1-1; Sequence=Displayed; CC Name=2; CC IsoId=Q96CJ1-2; Sequence=VSP_015310; CC -!- TISSUE SPECIFICITY: Expressed in heart, brain, placenta, lung, CC skeletal muscle, kidney, pancreas, spleen, prostate, testis, small CC intestine, colon, adrenal, bone marrow, lymphe node, spinal gland, CC stomach, thyroid, trachea, thymus, liver and leukocytes. CC -!- INDUCTION: By androgen. CC -!- SIMILARITY: Belongs to the EAF family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF517829; AAO63811.1; -; mRNA. DR EMBL; AY049020; AAL12223.1; -; mRNA. DR EMBL; AF217516; AAF67627.1; -; mRNA. DR EMBL; BC014209; AAH14209.1; -; mRNA. DR UniGene; Hs.477325; -. DR Ensembl; ENSG00000145088; Homo sapiens. DR HGNC; HGNC:23115; EAF2. DR MIM; 607659; gene. KW Activator; Alternative splicing; Apoptosis; Nuclear protein; KW Transcription; Transcription regulation. FT CHAIN 1 260 ELL-associated factor 2. FT /FTId=PRO_0000130337. FT REGION 17 104 Necessary for interaction with ELL. FT REGION 177 260 Necessary for transactivation activity. FT REGION 246 260 Necessary for interaction with TCEA1 and FT transactivation activity (By similarity). FT COMPBIAS 126 130 Poly-Gln. FT COMPBIAS 174 207 Ser-rich. FT VARSPLIC 1 130 Missing (in isoform 2). FT /FTId=VSP_015310. SQ SEQUENCE 260 AA; 28792 MW; 2CDCE96FA387C799 CRC64; MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY LEVGEGEQVT ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL EKLSSNITVK KTRVEGSSKI QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM DQMSSCDSSS DSKSSSSSSS EDSSSDSEDE DCKSSTSDTG NCVSGHPTMT QYRIPDIDAS HNRFRDNSGL LMNTLRNDLQ LSESGSDSDD //