ID FLOWR_DROME Reviewed; 194 AA. AC Q95T12; Q8IQM8; Q9VUU2; DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2001, sequence version 1. DT 22-FEB-2023, entry version 140. DE RecName: Full=Calcium channel flower {ECO:0000303|PubMed:19737521}; DE Short=3L5 {ECO:0000303|PubMed:19737521}; GN Name=fwe; Synonyms=flower; ORFNames=CG6151; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] {ECO:0000312|EMBL:AAN11767.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., RA Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [2] {ECO:0000305, ECO:0000312|EMBL:AAN11767.1} RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING. RC STRAIN=Berkeley; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [3] {ECO:0000305, ECO:0000312|EMBL:AAL25424.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL25424.1}; RC TISSUE=Embryo {ECO:0000269|PubMed:12537569}; RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080; RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., RA Celniker S.E.; RT "A Drosophila full-length cDNA resource."; RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002). RN [4] {ECO:0000305} RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE RP SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLU-79 AND GLY-128. RX PubMed=19737521; DOI=10.1016/j.cell.2009.06.033; RA Yao C.-K., Lin Y.Q., Ly C.V., Ohyama T., Haueter C.M., RA Moiseenkova-Bell V.Y., Wensel T.G., Bellen H.J.; RT "A synaptic vesicle-associated Ca2+ channel promotes endocytosis and RT couples exocytosis to endocytosis."; RL Cell 138:947-960(2009). CC -!- FUNCTION: A calcium channel that regulates synaptic endocytosis and CC hence couples exo- with endocytosis. Isoform A and isoform B are mainly CC required in the nervous system and necessary in photoreceptor cells. CC {ECO:0000269|PubMed:19737521}. CC -!- SUBUNIT: Homomultimer; Isoform B. {ECO:0000269|PubMed:19737521}. CC -!- INTERACTION: CC Q95T12; Q95T12: fwe; NbExp=7; IntAct=EBI-169467, EBI-169467; CC Q95T12-2; Q95T12-2: fwe; NbExp=5; IntAct=EBI-2497181, EBI-2497181; CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic CC vesicle membrane {ECO:0000269|PubMed:19737521}; Multi-pass membrane CC protein {ECO:0000269|PubMed:19737521}. Note=Upon fusion of the synaptic CC vesicle with the presynaptic membrane, protein is present in the CC periactive zones, where endocytosis is known to occur. {ECO:0000255, CC ECO:0000269|PubMed:19737521}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=A {ECO:0000269|PubMed:10731132, ECO:0000269|PubMed:19737521}; CC IsoId=Q95T12-1; Sequence=Displayed; CC Name=B {ECO:0000269|PubMed:10731132, ECO:0000269|PubMed:19737521}; CC IsoId=Q95T12-2; Sequence=VSP_053166; CC Name=C {ECO:0000269|PubMed:10731132, ECO:0000269|PubMed:19737521}; CC IsoId=Q95T12-3; Sequence=VSP_053165; CC -!- TISSUE SPECIFICITY: Isoform B is expressed in the neuropils of CC embryonic, larval, adult CNS, and R1-R6 terminals. Expression in the CC central nervous system (CNS) starts at embryonic stage 13. CC {ECO:0000269|PubMed:19737521}. CC -!- DISRUPTION PHENOTYPE: Impaired intracellular resting calcium levels and CC impaired endocytosis in presynaptic terminals, exocytosis is normal. CC Mutant boutons at the neuromuscular junctions exhibit a significant CC depletion in the number of synaptic vesicles. There are numerous extra CC boutons which are often small, clustered, and flowery in nature. Mutant CC nerve terminals display omega structures and collared pits. CC {ECO:0000269|PubMed:19737521}. CC -!- SIMILARITY: Belongs to the calcium channel flower family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE014296; AAF49581.1; -; Genomic_DNA. DR EMBL; AE014296; AAN11767.1; -; Genomic_DNA. DR EMBL; AE014296; AAN11768.1; -; Genomic_DNA. DR EMBL; AY060385; AAL25424.1; -; mRNA. DR RefSeq; NP_648804.1; NM_140547.5. [Q95T12-1] DR RefSeq; NP_730071.1; NM_168632.4. [Q95T12-3] DR RefSeq; NP_730072.1; NM_168633.4. [Q95T12-2] DR AlphaFoldDB; Q95T12; -. DR BioGRID; 65034; 8. DR IntAct; Q95T12; 27. DR STRING; 7227.FBpp0075297; -. DR TCDB; 1.A.55.1.2; the synaptic vesicle-associated ca(2+) channel, flower (flower) family. DR SwissPalm; Q95T12; -. DR PaxDb; Q95T12; -. DR DNASU; 39720; -. DR EnsemblMetazoa; FBtr0075541; FBpp0075296; FBgn0261722. [Q95T12-2] DR EnsemblMetazoa; FBtr0075542; FBpp0075297; FBgn0261722. [Q95T12-1] DR EnsemblMetazoa; FBtr0075543; FBpp0075298; FBgn0261722. [Q95T12-3] DR GeneID; 39720; -. DR KEGG; dme:Dmel_CG6151; -. DR UCSC; CG6151-RA; d. melanogaster. [Q95T12-1] DR UCSC; CG6151-RB; d. melanogaster. DR UCSC; CG6151-RC; d. melanogaster. DR AGR; FB:FBgn0261722; -. DR CTD; 39720; -. DR FlyBase; FBgn0261722; fwe. DR VEuPathDB; VectorBase:FBgn0261722; -. DR eggNOG; KOG4085; Eukaryota. DR GeneTree; ENSGT00390000000529; -. DR HOGENOM; CLU_108196_0_0_1; -. DR InParanoid; Q95T12; -. DR OMA; CEAPCCC; -. DR OrthoDB; 5397038at2759; -. DR PhylomeDB; Q95T12; -. DR BioGRID-ORCS; 39720; 0 hits in 1 CRISPR screen. DR GenomeRNAi; 39720; -. DR PRO; PR:Q95T12; -. DR Proteomes; UP000000803; Chromosome 3L. DR Bgee; FBgn0261722; Expressed in wing disc and 31 other tissues. DR ExpressionAtlas; Q95T12; baseline and differential. DR Genevisible; Q95T12; DM. DR GO; GO:0005886; C:plasma membrane; IDA:FlyBase. DR GO; GO:0030672; C:synaptic vesicle membrane; IDA:UniProtKB. DR GO; GO:0005262; F:calcium channel activity; IDA:FlyBase. DR GO; GO:0042802; F:identical protein binding; IPI:IntAct. DR GO; GO:0150008; P:bulk synaptic vesicle endocytosis; IMP:FlyBase. DR GO; GO:0070588; P:calcium ion transmembrane transport; IMP:FlyBase. DR GO; GO:0006816; P:calcium ion transport; IDA:FlyBase. DR GO; GO:0035212; P:cell competition in a multicellular organism; IMP:FlyBase. DR GO; GO:0150007; P:clathrin-dependent synaptic vesicle endocytosis; IMP:FlyBase. DR GO; GO:0046530; P:photoreceptor cell differentiation; IMP:UniProtKB. DR GO; GO:0043525; P:positive regulation of neuron apoptotic process; IMP:FlyBase. DR GO; GO:0099533; P:positive regulation of presynaptic cytosolic calcium concentration; IMP:FlyBase. DR GO; GO:0042981; P:regulation of apoptotic process; IMP:FlyBase. DR GO; GO:0048488; P:synaptic vesicle endocytosis; IMP:UniProtKB. DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central. DR InterPro; IPR019365; TVP18/Ca-channel_flower. DR PANTHER; PTHR13314:SF2; CALCIUM CHANNEL FLOWER HOMOLOG; 1. DR PANTHER; PTHR13314; UNCHARACTERIZED; 1. DR Pfam; PF10233; Cg6151-P; 1. DR SMART; SM01077; Cg6151-P; 1. PE 1: Evidence at protein level; KW Alternative splicing; Calcium; Calcium channel; Calcium transport; KW Cytoplasmic vesicle; Endocytosis; Ion channel; Ion transport; Membrane; KW Reference proteome; Synapse; Transmembrane; Transmembrane helix; Transport. FT CHAIN 1..194 FT /note="Calcium channel flower" FT /id="PRO_0000389234" FT TRANSMEM 35..55 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 66..88 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 107..127 FT /note="Helical" FT /evidence="ECO:0000255" FT SITE 79 FT /note="Calcium ion selectivity" FT /evidence="ECO:0000269|PubMed:19737521" FT VAR_SEQ 147..194 FT /note="KASAEDMRAAAQQTFGGNTPAQTNDRAGIVNNAQPFSFTGAVGTDSNV -> FT NKHIFLVRNYI (in isoform C)" FT /evidence="ECO:0000303|PubMed:10731132, FT ECO:0000303|PubMed:19737521" FT /id="VSP_053165" FT VAR_SEQ 150..194 FT /note="AEDMRAAAQQTFGGNTPAQTNDRAGIVNNAQPFSFTGAVGTDSNV -> RED FT MAAAATSPTQMAGSQAGGQMQMGGDQHITLMEDPDVWRPT (in isoform B)" FT /evidence="ECO:0000303|PubMed:10731132, FT ECO:0000303|PubMed:19737521" FT /id="VSP_053166" FT MUTAGEN 79 FT /note="E->Q: Heterologous expression in salivary glands FT shows inhibition of calcium influx." FT /evidence="ECO:0000269|PubMed:19737521" FT MUTAGEN 128 FT /note="G->D: In allele DB25; defect in synaptic FT transmission and homozygous lethal." FT /evidence="ECO:0000269|PubMed:19737521" SQ SEQUENCE 194 AA; 20620 MW; 2D2817158AD73A57 CRC64; MSFAEKITGL LARPNQQDPI GPEQPWYLKY GSRLLGIVAA FFAILFGLWN VFSIITLSVS CLVAGILQMV AGFVVMLLEA PCCFVCFGQV NEIAEKVESK PLYFRAGLYI AMAIPPIILC FGLASLFGSG LIFGTGVVYG MMALGKKASA EDMRAAAQQT FGGNTPAQTN DRAGIVNNAQ PFSFTGAVGT DSNV //