ID ARFB_ARATH Reviewed; 859 AA. AC Q94JM3; O23671; Q56YL7; Q56YP4; Q56YR7; Q9C5W5; Q9FIT6; DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot. DT 13-APR-2004, sequence version 2. DT 15-JUN-2010, entry version 65. DE RecName: Full=Auxin response factor 2; DE AltName: Full=ARF1-binding protein; DE Short=ARF1-BP; DE AltName: Full=Protein MEGAINTEGUMENTA; GN Name=ARF2; Synonyms=MNT; OrderedLocusNames=At5g62000; GN ORFNames=MTG10.3; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; malvids; Brassicales; Brassicaceae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Columbia; RX PubMed=15659631; DOI=10.1105/tpc.104.028316; RA Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., RA Chang C., Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., RA Quach H., Smith A., Yu G., Theologis A.; RT "Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family RT members in Arabidopsis thaliana: unique and overlapping functions of RT ARF7 and ARF19."; RL Plant Cell 17:444-463(2005). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX MEDLINE=99087489; PubMed=9872454; DOI=10.1093/dnares/5.5.297; RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., RA Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. RT Sequence features of the regions of 1,013,767 bp covered by sixteen RT physically assigned P1 and TAC clones."; RL DNA Res. 5:297-308(1998). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K., RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., RA Hayashizaki Y., Shinozaki K.; RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."; RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [MRNA] OF 394-859. RC STRAIN=cv. Columbia; RX MEDLINE=97334443; PubMed=9188533; DOI=10.1126/science.276.5320.1865; RA Ulmasov T., Hagen G., Guilfoyle T.J.; RT "ARF1, a transcription factor that binds to auxin response elements."; RL Science 276:1865-1868(1997). RN [6] RP NUCLEOTIDE SEQUENCE OF 406-859. RC STRAIN=cv. Columbia; RA Sessa G., Carabelli M., Ciarbelli A.R., Ruzza V., Steindler C., RA Ruberti I.; RT "Nucleotide sequence of the Arabidopsis IAA30."; RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases. RN [7] RP DIMERIZATION, AND TISSUE SPECIFICITY. RX MEDLINE=99406518; PubMed=10476078; RX DOI=10.1046/j.1365-313X.1999.00538.x; RA Ulmasov T., Hagen G., Guilfoyle T.J.; RT "Dimerization and DNA binding of auxin response factors."; RL Plant J. 19:309-319(1999). RN [8] RP GENE FAMILY, NOMENCLATURE, AND FUNCTION. RX MEDLINE=22031747; PubMed=12036261; DOI=10.1023/A:1015207114117; RA Hagen G., Guilfoyle T.J.; RT "Auxin-responsive gene expression: genes, promoters and regulatory RT factors."; RL Plant Mol. Biol. 49:373-385(2002). RN [9] RP FUNCTION, AND DEVELOPMENTAL STAGE. RX PubMed=16176952; DOI=10.1242/dev.02012; RA Ellis C.M., Nagpal P., Young J.C., Hagen G., Guilfoyle T.J., RA Reed J.W.; RT "AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence RT and floral organ abscission in Arabidopsis thaliana."; RL Development 132:4563-4574(2005). RN [10] RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION RP PHENOTYPE. RX PubMed=15960614; DOI=10.1111/j.1365-313X.2005.02426.x; RA Okushima Y., Mitina I., Quach H.L., Theologis A.; RT "AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental RT regulator."; RL Plant J. 43:29-46(2005). RN [11] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=16339187; DOI=10.1242/dev.02194; RA Schruff M.C., Spielman M., Tiwari S., Adams S., Fenby N., Scott R.J.; RT "The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin RT signalling, cell division, and the size of seeds and other organs."; RL Development 133:251-261(2006). CC -!- FUNCTION: Auxin response factors (ARFs) are transcriptional CC factors that binds specifically to the DNA sequence 5'-TGTCTC-3' CC found in the auxin-responsive promoter elements (AuxREs). Could CC act as transcriptional activator or repressor. Formation of CC heterodimers with Aux/IAA proteins may alter their ability to CC modulate early auxin response genes expression. Promotes CC flowering, stamen development, floral organ abscission and fruit CC dehiscence. Functions independently of ethylene and cytokinin CC response pathways. May act as a repressor of cell division and CC organ growth. CC -!- SUBUNIT: Homo and heterodimers. Interacts with ARF1. CC -!- INTERACTION: CC Q39011:ASK7; NbExp=3; IntAct=EBI-1799262, EBI-1798250; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- TISSUE SPECIFICITY: Expressed in the whole plant. CC -!- DEVELOPMENTAL STAGE: Expressed in the sepals and carpels of young CC flower buds. At stage 10 of flower development, expression in the CC carpels becomes restricted to the style. Also expressed in anthers CC and filaments. At stage 13, expressed in the region at the top of CC the pedicel, including the abscission zone. Expressed in CC developing siliques. CC -!- DOMAIN: Interactions between auxin response factors (ARFs) and CC Aux/IAA proteins occur through their C-terminal dimerization CC domains III and IV. CC -!- DISRUPTION PHENOTYPE: Large, dark green rosette leaves, delayed CC flowering, thick and long inflorescence, abnormal flower CC morphology and sterility in early formed flowers, but fertility in CC late-formed flowers. Delayed senescence and abscission. Increased CC seed size and weight, and extra cell division and expansion in CC many organs. CC -!- SIMILARITY: Belongs to the ARF family. CC -!- SIMILARITY: Contains 1 Aux/IAA-ARF domain. CC -!- SIMILARITY: Contains 1 TF-B3 DNA-binding domain. CC -!- CAUTION: Was originally (Ref.4) erroneously termed IAA26 and CC IAA30. CC -!- SEQUENCE CAUTION: CC Sequence=AAC49752.1; Type=Erroneous initiation; CC Sequence=AAK55665.1; Type=Frameshift; Positions=657; CC Sequence=AAN46837.1; Type=Frameshift; Positions=657; CC Sequence=CAD29696.1; Type=Erroneous initiation; CC Sequence=CAD30210.1; Type=Erroneous initiation; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY669787; AAT67071.1; -; mRNA. DR EMBL; AB016880; BAB10162.1; -; Genomic_DNA. DR EMBL; AF336918; AAG53999.1; -; mRNA. DR EMBL; AF378862; AAK55665.1; ALT_FRAME; mRNA. DR EMBL; BT000784; AAN31923.1; -; mRNA. DR EMBL; BT001072; AAN46837.1; ALT_FRAME; mRNA. DR EMBL; AK221252; BAD93891.1; -; mRNA. DR EMBL; AK221254; BAD93897.1; -; mRNA. DR EMBL; AK221274; BAD93959.1; -; mRNA. DR EMBL; AK221277; BAD93968.1; -; mRNA. DR EMBL; AK221282; BAD93985.1; -; mRNA. DR EMBL; AK221305; BAD94058.1; -; mRNA. DR EMBL; U89771; AAC49752.1; ALT_INIT; mRNA. DR EMBL; AJ441313; CAD29696.1; ALT_INIT; mRNA. DR EMBL; AJ458328; CAD30210.1; ALT_INIT; mRNA. DR IPI; IPI00518739; -. DR RefSeq; NP_201006.2; -. DR RefSeq; NP_851244.1; -. DR RefSeq; NP_974980.1; -. DR UniGene; At.21704; -. DR SMR; Q94JM3; 162-269. DR IntAct; Q94JM3; 2. DR STRING; Q94JM3; -. DR PRIDE; Q94JM3; -. DR EnsemblPlants; AT5G62000.1-TAIR; AT5G62000.1-P; AT5G62000-TAIR-G. DR EnsemblPlants; AT5G62000.2-TAIR; AT5G62000.2-P; AT5G62000-TAIR-G. DR EnsemblPlants; AT5G62000.3-TAIR; AT5G62000.3-P; AT5G62000-TAIR-G. DR GeneID; 836321; -. DR GenomeReviews; BA000015_GR; AT5G62000. DR KEGG; ath:AT5G62000; -. DR NMPDR; fig|3702.1.peg.28232; -. DR GeneFarm; 3364; 345. DR TAIR; At5g62000; -. DR InParanoid; Q94JM3; -. DR OMA; ATAWHAI; -. DR ArrayExpress; Q94JM3; -. DR Genevestigator; Q94JM3; -. DR GermOnline; AT5G62000; Arabidopsis thaliana. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro. DR GO; GO:0009734; P:auxin mediated signaling pathway; IEA:UniProtKB-KW. DR GO; GO:0010227; P:floral organ abscission; IMP:TAIR. DR GO; GO:0010047; P:fruit dehiscence; IMP:TAIR. DR GO; GO:0010150; P:leaf senescence; IMP:TAIR. DR GO; GO:0008285; P:negative regulation of cell proliferation; IMP:TAIR. DR GO; GO:0045892; P:negative regulation of transcription, DNA-d...; TAS:TAIR. DR GO; GO:0048481; P:ovule development; IMP:TAIR. DR GO; GO:0009911; P:positive regulation of flower development; IMP:TAIR. DR GO; GO:0006350; P:transcription; IEA:UniProtKB-KW. DR InterPro; IPR011525; AuxIAA_ARF_dimer. DR InterPro; IPR010525; Auxin_resp. DR InterPro; IPR003340; TF_B3. DR Pfam; PF06507; Auxin_resp; 1. DR Pfam; PF02362; B3; 1. DR PROSITE; PS50863; B3; 1. DR PROSITE; PS50962; IAA_ARF; 1. PE 1: Evidence at protein level; KW Auxin signaling pathway; Complete proteome; DNA-binding; Nucleus; KW Transcription; Transcription regulation. FT CHAIN 1 859 Auxin response factor 2. FT /FTId=PRO_0000111506. FT DOMAIN 734 816 Aux/IAA-ARF. FT DNA_BIND 164 266 TF-B3. FT CONFLICT 491 492 RR -> KK (in Ref. 5; AAC49752 and 6; FT CAD29696/CAD30210). FT CONFLICT 520 520 D -> E (in Ref. 5; AAC49752 and 6; FT CAD29696/CAD30210). FT CONFLICT 596 596 V -> A (in Ref. 4; BAD93968). FT CONFLICT 675 675 R -> K (in Ref. 5; AAC49752 and 6; FT CAD29696/CAD30210). FT CONFLICT 823 823 P -> Q (in Ref. 5; AAC49752 and 6; FT CAD29696/CAD30210). FT CONFLICT 826 826 L -> V (in Ref. 4; BAD93959/BAD93897/ FT BAD93891). SQ SEQUENCE 859 AA; 95701 MW; 6B75171D7FF0E66B CRC64; MASSEVSMKG NRGGDNFSSS GFSDPKETRN VSVAGEGQKS NSTRSAAAER ALDPEAALYR ELWHACAGPL VTVPRQDDRV FYFPQGHIEQ VEASTNQAAE QQMPLYDLPS KLLCRVINVD LKAEADTDEV YAQITLLPEA NQDENAIEKE APLPPPPRFQ VHSFCKTLTA SDTSTHGGFS VLRRHADECL PPLDMSRQPP TQELVAKDLH ANEWRFRHIF RGQPRRHLLQ SGWSVFVSSK RLVAGDAFIF LRGENGELRV GVRRAMRQQG NVPSSVISSH SMHLGVLATA WHAISTGTMF TVYYKPRTSP SEFIVPFDQY MESVKNNYSI GMRFKMRFEG EEAPEQRFTG TIVGIEESDP TRWPKSKWRS LKVRWDETSS IPRPDRVSPW KVEPALAPPA LSPVPMPRPK RPRSNIAPSS PDSSMLTREG TTKANMDPLP ASGLSRVLQG QEYSTLRTKH TESVECDAPE NSVVWQSSAD DDKVDVVSGS RRYGSENWMS SARHEPTYTD LLSGFGTNID PSHGQRIPFY DHSSSPSMPA KRILSDSEGK FDYLANQWQM IHSGLSLKLH ESPKVPAATD ASLQGRCNVK YSEYPVLNGL STENAGGNWP IRPRALNYYE EVVNAQAQAQ AREQVTKQPF TIQEETAKSR EGNCRLFGIP LTNNMNGTDS TMSQRNNLND AAGLTQIASP KVQDLSDQSK GSKSTNDHRE QGRPFQTNNP HPKDAQTKTN SSRSCTKVHK QGIALGRSVD LSKFQNYEEL VAELDRLFEF NGELMAPKKD WLIVYTDEEN DMMLVGDDPW QEFCCMVRKI FIYTKEEVRK MNPGTLSCRS EEEAVVGEGS DAKDAKSASN PSLSSAGNS //