ID TPSD6_ABIGR Reviewed; 618 AA. AC Q948Z0; DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2001, sequence version 1. DT 03-AUG-2022, entry version 84. DE RecName: Full=Camphene synthase, chloroplastic; DE EC=4.2.3.117; DE AltName: Full=(-)-(1S,4R)-camphene synthase; DE AltName: Full=Agg-cam; DE Flags: Precursor; GN Name=ag6; Synonyms=AG6.5; OS Abies grandis (Grand fir) (Pinus grandis). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Abies. OX NCBI_TaxID=46611; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=9268308; DOI=10.1074/jbc.272.35.21784; RA Bohlmann J., Steele C.L., Croteau R.B.; RT "Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, RT characterization, and functional expression of myrcene synthase, (-)-(4S)- RT limonene synthase, and (-)-(1S,5S)-pinene synthase."; RL J. Biol. Chem. 272:21784-21792(1997). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY. RC TISSUE=Stem; RX PubMed=10441373; DOI=10.1006/abbi.1999.1332; RA Bohlmann J., Phillips M., Ramachandiran V., Katoh S., Croteau R.B.; RT "cDNA cloning, characterization, and functional expression of four new RT monoterpene synthase members of the Tpsd gene family from grand fir (Abies RT grandis)."; RL Arch. Biochem. Biophys. 368:232-243(1999). RN [3] RP GENE FAMILY, NOMENCLATURE, AND FUNCTION. RX PubMed=9539701; DOI=10.1073/pnas.95.8.4126; RA Bohlmann J., Meyer-Gauen G., Croteau R.B.; RT "Plant terpenoid synthases: molecular biology and phylogenetic analysis."; RL Proc. Natl. Acad. Sci. U.S.A. 95:4126-4133(1998). RN [4] RP GENE FAMILY, NOMENCLATURE, AND FUNCTION. RX PubMed=11404343; DOI=10.1093/genetics/158.2.811; RA Trapp S.C., Croteau R.B.; RT "Genomic organization of plant terpene synthases and molecular evolutionary RT implications."; RL Genetics 158:811-832(2001). CC -!- FUNCTION: Involved in defensive oleoresin formation in conifers in CC response to insect attack or other injury. Involved in monoterpene CC (C10) olefins biosynthesis. {ECO:0000269|PubMed:10441373, CC ECO:0000269|PubMed:11404343, ECO:0000269|PubMed:9539701}. CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-geranyl diphosphate = (1S,4R)-camphene + diphosphate; CC Xref=Rhea:RHEA:25484, ChEBI:CHEBI:89, ChEBI:CHEBI:33019, CC ChEBI:CHEBI:58057; EC=4.2.3.117; CC Evidence={ECO:0000269|PubMed:10441373}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250}; CC -!- COFACTOR: CC Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000250}; CC -!- PATHWAY: Terpene metabolism; oleoresin biosynthesis. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}. CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for CC the catalytic activity, presumably through binding to Mg(2+). CC -!- MISCELLANEOUS: The conserved 59-Arg-Arg-60 motif may play a role in the CC isomerization step of the terpenoid cyclization reaction sequence. CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsd subfamily. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U87910; AAB70707.1; -; mRNA. DR AlphaFoldDB; Q948Z0; -. DR SMR; Q948Z0; -. DR PRIDE; Q948Z0; -. DR KEGG; ag:AAB70707; -. DR BioCyc; MetaCyc:AG6-MON; -. DR BRENDA; 4.2.3.117; 2. DR BRENDA; 4.2.3.119; 2. DR UniPathway; UPA00924; -. DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell. DR GO; GO:0102703; F:camphene synthase activity; IEA:UniProtKB-EC. DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro. DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro. DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro. DR CDD; cd00684; Terpene_cyclase_plant_C1; 1. DR Gene3D; 1.10.600.10; -; 1. DR Gene3D; 1.50.10.130; -; 1. DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf. DR InterPro; IPR034741; Terpene_cyclase-like_1_C. DR InterPro; IPR044814; Terpene_cyclase_plant_C1. DR InterPro; IPR001906; Terpene_synth_N. DR InterPro; IPR036965; Terpene_synth_N_sf. DR InterPro; IPR005630; Terpene_synthase_metal-bd. DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase. DR Pfam; PF01397; Terpene_synth; 1. DR Pfam; PF03936; Terpene_synth_C; 1. DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1. DR SUPFAM; SSF48239; SSF48239; 1. DR SUPFAM; SSF48576; SSF48576; 1. PE 1: Evidence at protein level; KW Chloroplast; Lyase; Magnesium; Manganese; Metal-binding; Plastid; KW Transit peptide. FT TRANSIT 1..51 FT /note="Chloroplast" FT /evidence="ECO:0000255" FT CHAIN 52..618 FT /note="Camphene synthase, chloroplastic" FT /id="PRO_0000033629" FT MOTIF 369..373 FT /note="DDXXD motif" FT BINDING 369 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 369 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="2" FT /evidence="ECO:0000250" FT BINDING 373 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 373 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="2" FT /evidence="ECO:0000250" FT BINDING 521 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="3" FT /evidence="ECO:0000250" SQ SEQUENCE 618 AA; 70750 MW; B07B5185CE5C4CE1 CRC64; MALLSITPLV SRSCLSSSHE IKALRRTIPT LGICRPGKSV AHSINMCLTS VASTDSVQRR VGNYHSNLWD DDFIQSLIST PYGAPDYRER ADRLIGEVKD IMFNFKSLED GGNDLLQRLL LVDDVERLGI DRHFKKEIKT ALDYVNSYWN EKGIGCGRES VVTDLNSTAL GLRTLRLHGY TVSSDVLNVF KDKNGQFSST ANIQIEGEIR GVLNLFRASL VAFPGEKVMD EAETFSTKYL REALQKIPAS SILSLEIRDV LEYGWHTNLP RLEARNYMDV FGQHTKNKNA AEKLLELAKL EFNIFHSLQE RELKHVSRWW KDSGSPEMTF CRHRHVEYYA LASCIAFEPQ HSGFRLGFTK MSHLITVLDD MYDVFGTVDE LELFTATIKR WDPSAMECLP EYMKGVYMMV YHTVNEMARV AEKAQGRDTL NYARQAWEAC FDSYMQEAKW IATGYLPTFE EYLENGKVSS AHRPCALQPI LTLDIPFPDH ILKEVDFPSK LNDLICIILR LRGDTRCYKA DRARGEEASS ISCYMKDNPG LTEEDALNHI NFMIRDAIRE LNWELLKPDN SVPITSKKHA FDISRVWHHG YRYRDGYSFA NVETKSLVMR TVIEPVPL //