ID GLYK_ARATH Reviewed; 456 AA. AC Q944I4; A8MQE0; Q8LFL2; Q9C5L9; Q9C967; DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot. DT 30-AUG-2005, sequence version 2. DT 07-APR-2021, entry version 125. DE RecName: Full=D-glycerate 3-kinase, chloroplastic {ECO:0000305}; DE Short=AtGLYK {ECO:0000303|PubMed:15980259}; DE EC=2.7.1.31 {ECO:0000269|PubMed:15980259}; DE Flags: Precursor; GN Name=GLYK {ECO:0000303|PubMed:15980259}; GN OrderedLocusNames=At1g80380 {ECO:0000312|Araport:AT1G80380}; GN ORFNames=F5I6.13 {ECO:0000312|EMBL:AAG52441.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., RA Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."; RL Nature 408:816-820(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., RA Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [5] RP CATALYTIC ACTIVITY, IDENTIFICATION BY MASS SPECTROMETRY, AND FUNCTION. RX PubMed=15980259; DOI=10.1105/tpc.105.033993; RA Boldt R., Edner C., Kolukisaoglu U., Hagemann M., Weckwerth W., RA Wienkoop S., Morgenthal K., Bauwe H.; RT "D-glycerate 3-kinase, the last unknown enzyme in the photorespiratory RT cycle in Arabidopsis, belongs to a novel kinase family."; RL Plant Cell 17:2413-2420(2005). RN [6] RP FUNCTION (ISOFORM 3), SUBCELLULAR LOCATION (ISOFORMS 1 AND 3), ALTERNATIVE RP SPLICING, AND INDUCTION. RX PubMed=29129375; DOI=10.1016/j.cell.2017.10.018; RA Ushijima T., Hanada K., Gotoh E., Yamori W., Kodama Y., Tanaka H., RA Kusano M., Fukushima A., Tokizawa M., Yamamoto Y.Y., Tada Y., Suzuki Y., RA Matsushita T.; RT "Light controls protein localization through phytochrome-mediated RT alternative promoter selection."; RL Cell 0:0-0(2017). CC -!- FUNCTION: Catalyzes the concluding reaction of the photorespiratory C2 CC cycle, an indispensable ancillary metabolic pathway to the CC photosynthetic C3 cycle that enables land plants to grow in an oxygen- CC containing atmosphere. {ECO:0000269|PubMed:15980259}. CC -!- FUNCTION: [Isoform 3]: Cytoplasmic D-glycerate 3-kinase that CC constitutes a photorespiratory bypass that alleviates fluctuating CC light-induced photoinhibition (PubMed:29129375). CC {ECO:0000269|PubMed:29129375}. CC -!- CATALYTIC ACTIVITY: CC Reaction=(R)-glycerate + ATP = (2R)-3-phosphoglycerate + ADP + H(+); CC Xref=Rhea:RHEA:23516, ChEBI:CHEBI:15378, ChEBI:CHEBI:16659, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58272, ChEBI:CHEBI:456216; CC EC=2.7.1.31; Evidence={ECO:0000269|PubMed:15980259}; CC -!- PATHWAY: Photosynthesis; photorespiration; 3-phospho-D-glycerate from CC glycine: step 4/4. {ECO:0000305}. CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Plastid, chloroplast CC {ECO:0000269|PubMed:29129375}. Note=Expressed in the light. CC {ECO:0000269|PubMed:29129375}. CC -!- SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm CC {ECO:0000269|PubMed:29129375}. Note=Expressed in the dark. CC {ECO:0000269|PubMed:29129375}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q944I4-1; Sequence=Displayed; CC Name=2; CC IsoId=Q944I4-2; Sequence=VSP_015208; CC Name=3; CC IsoId=Q944I4-3; Sequence=VSP_059299; CC -!- INDUCTION: Isoform 1: Expressed in the light (PubMed:29129375). Isoform CC 3: Expressed in the dark (PubMed:29129375). CC {ECO:0000269|PubMed:29129375}. CC -!- MISCELLANEOUS: [Isoform 1]: Expressed in the light. CC {ECO:0000269|PubMed:29129375}. CC -!- MISCELLANEOUS: [Isoform 3]: Expressed in the dark. CC {ECO:0000269|PubMed:29129375}. CC -!- SIMILARITY: Belongs to the GLYK kinase family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAG52441.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AC018848; AAG52441.1; ALT_SEQ; Genomic_DNA. DR EMBL; CP002684; AEE36393.1; -; Genomic_DNA. DR EMBL; CP002684; AEE36394.1; -; Genomic_DNA. DR EMBL; CP002684; AEE36395.1; -; Genomic_DNA. DR EMBL; AF360156; AAK25866.1; -; mRNA. DR EMBL; AF428401; AAL16169.1; -; mRNA. DR EMBL; AY056310; AAL07159.1; -; mRNA. DR EMBL; AY084779; AAM61346.1; -; mRNA. DR PIR; E96835; E96835. DR RefSeq; NP_001077855.1; NM_001084386.2. [Q944I4-3] DR RefSeq; NP_565237.1; NM_106687.2. [Q944I4-2] DR RefSeq; NP_849912.1; NM_179581.3. [Q944I4-1] DR SMR; Q944I4; -. DR STRING; 3702.AT1G80380.2; -. DR iPTMnet; Q944I4; -. DR PaxDb; Q944I4; -. DR PRIDE; Q944I4; -. DR ProteomicsDB; 247370; -. [Q944I4-1] DR EnsemblPlants; AT1G80380.1; AT1G80380.1; AT1G80380. [Q944I4-2] DR EnsemblPlants; AT1G80380.2; AT1G80380.2; AT1G80380. [Q944I4-1] DR EnsemblPlants; AT1G80380.3; AT1G80380.3; AT1G80380. [Q944I4-3] DR GeneID; 844378; -. DR Gramene; AT1G80380.1; AT1G80380.1; AT1G80380. [Q944I4-2] DR Gramene; AT1G80380.2; AT1G80380.2; AT1G80380. [Q944I4-1] DR Gramene; AT1G80380.3; AT1G80380.3; AT1G80380. [Q944I4-3] DR KEGG; ath:AT1G80380; -. DR Araport; AT1G80380; -. DR TAIR; locus:2034260; AT1G80380. DR eggNOG; KOG2878; Eukaryota. DR InParanoid; Q944I4; -. DR PhylomeDB; Q944I4; -. DR BioCyc; ARA:AT1G80380-MONOMER; -. DR BioCyc; MetaCyc:AT1G80380-MONOMER; -. DR BRENDA; 2.7.1.31; 399. DR UniPathway; UPA00951; UER00917. DR PRO; PR:Q944I4; -. DR Proteomes; UP000006548; Chromosome 1. DR ExpressionAtlas; Q944I4; baseline and differential. DR Genevisible; Q944I4; AT. DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB. DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR. DR GO; GO:0009570; C:chloroplast stroma; IDA:TAIR. DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB. DR GO; GO:0005739; C:mitochondrion; HDA:TAIR. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0008887; F:glycerate kinase activity; IDA:TAIR. DR GO; GO:0016301; F:kinase activity; IBA:GO_Central. DR GO; GO:0009854; P:oxidative photosynthetic carbon pathway; IEA:UniProtKB-UniPathway. DR GO; GO:0009853; P:photorespiration; IMP:TAIR. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR006083; PRK/URK. DR Pfam; PF00485; PRK; 1. DR SUPFAM; SSF52540; SSF52540; 1. PE 1: Evidence at protein level; KW Alternative splicing; ATP-binding; Chloroplast; Cytoplasm; KW Glycolate pathway; Kinase; Nucleotide-binding; Photorespiration; Plastid; KW Reference proteome; Transferase; Transit peptide. FT TRANSIT 1..63 FT /note="Chloroplast" FT /evidence="ECO:0000255" FT CHAIN 64..456 FT /note="D-glycerate 3-kinase, chloroplastic" FT /id="PRO_0000012282" FT NP_BIND 215..222 FT /note="ATP" FT /evidence="ECO:0000255" FT VAR_SEQ 1..92 FT /note="Missing (in isoform 3)" FT /id="VSP_059299" FT VAR_SEQ 206..299 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000305" FT /id="VSP_015208" FT CONFLICT 188 FT /note="W -> C (in Ref. 4; AAM61346)" FT /evidence="ECO:0000305" FT CONFLICT 239 FT /note="S -> P (in Ref. 3; AAL16169)" FT /evidence="ECO:0000305" SQ SEQUENCE 456 AA; 51018 MW; 5961E0FC6E2220FB CRC64; MAVAISGSSL ISSTLQHYNN RIYIRDYPIP CHSSNPICNS FNFKRRSFSP SSPKFNDHVV NPSSSYLSSK LSPIRTHSSF AACGCSWIQD NSMVHDYATT TNGTSKRCSA LPTTNTVDVS SVSDLFEFIC SGPLVNKIGI TPQRVGQSID KWLLYGSQLC RLFQLNELKL TIPQKARLYH YYIPVFIWCE DQIALHNSKF KDGDDVPPLV IGFSAPQGCG KTTLVFALDY LFKTTKKKSA TISVDDFYLT AEGQAELRKK NPGNALLEYR GNAGSHDLKL SVETLEALSK LTKEGLKMKV PRYNKSAYSG RGDRADSSTW PEVEGPLSVI LFEGWMLGFK PLPADVVKAV DPQLEVVNKN LEAYYDAWDK YIDAWVVIKI QDPSYVYRWR LQAEIAMRQD GQAGMSDEEV NDFVSRYLPA YKAYLPTLYA EGPSGSDPDR VLAIDIDEER NPILAN //