ID SPP3A_ARATH Reviewed; 425 AA. AC Q93WU4; Q9M379; DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot. DT 23-SEP-2008, sequence version 2. DT 28-FEB-2018, entry version 88. DE RecName: Full=Probable sucrose-phosphatase 3a; DE Short=AtSPP3a; DE EC=3.1.3.24; GN Name=SPP3A; Synonyms=SPP3; OrderedLocusNames=At3g54270; GN ORFNames=F24B22.230; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; OC Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130713; DOI=10.1038/35048706; RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., RA Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., RA Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., RA De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P., RA Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., RA Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., RA Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., RA Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., RA Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., RA Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., RA Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., RA Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., RA Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., RA Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., RA Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., RA Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., RA Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., RA Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., RA Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., RA Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., RA Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., RA Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., RA Watanabe A., Yamada M., Yasuda M., Tabata S.; RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis RT thaliana."; RL Nature 408:820-822(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Portal (Araport); RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [MRNA] OF 48-425. RC STRAIN=cv. Columbia; TISSUE=Seed; RX PubMed=11050182; DOI=10.1073/pnas.230430197; RA Lunn J.E., Ashton A.R., Hatch M.D., Heldt H.W.; RT "Purification, molecular cloning, and sequence analysis of sucrose-6F- RT phosphate phosphohydrolase from plants."; RL Proc. Natl. Acad. Sci. U.S.A. 97:12914-12919(2000). RN [4] RP GENE FAMILY, AND NOMENCLATURE. RX PubMed=12559580; DOI=10.1016/S0378-1119(02)01177-0; RA Lunn J.E.; RT "Sucrose-phosphatase gene families in plants."; RL Gene 303:187-196(2003). CC -!- FUNCTION: Catalyzes the final step of sucrose synthesis. CC {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: Sucrose 6(F)-phosphate + H(2)O = sucrose + CC phosphate. CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; CC -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from CC D-fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2. CC -!- SUBUNIT: Homodimer. {ECO:0000250}. CC -!- SIMILARITY: Belongs to the sucrose phosphatase family. CC {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=CAB71001.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL132957; CAB71001.1; ALT_SEQ; Genomic_DNA. DR EMBL; CP002686; AEE79206.1; -; Genomic_DNA. DR EMBL; AF434711; AAL30747.1; -; mRNA. DR PIR; T47586; T47586. DR RefSeq; NP_190995.2; NM_115287.4. DR UniGene; At.27489; -. DR ProteinModelPortal; Q93WU4; -. DR SMR; Q93WU4; -. DR STRING; 3702.AT3G54270.1; -. DR PaxDb; Q93WU4; -. DR EnsemblPlants; AT3G54270.1; AT3G54270.1; AT3G54270. DR GeneID; 824594; -. DR Gramene; AT3G54270.1; AT3G54270.1; AT3G54270. DR KEGG; ath:AT3G54270; -. DR Araport; AT3G54270; -. DR TAIR; locus:2080295; AT3G54270. DR eggNOG; ENOG410JS53; Eukaryota. DR eggNOG; COG0561; LUCA. DR HOGENOM; HOG000272726; -. DR InParanoid; Q93WU4; -. DR KO; K07024; -. DR OMA; KFRIWLD; -. DR OrthoDB; EOG093609AY; -. DR PhylomeDB; Q93WU4; -. DR BioCyc; ARA:AT3G54270-MONOMER; -. DR UniPathway; UPA00371; UER00546. DR PRO; PR:Q93WU4; -. DR Proteomes; UP000006548; Chromosome 3. DR ExpressionAtlas; Q93WU4; baseline and differential. DR Genevisible; Q93WU4; AT. DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro. DR GO; GO:0050307; F:sucrose-phosphate phosphatase activity; IEA:UniProtKB-EC. DR GO; GO:0016757; F:transferase activity, transferring glycosyl groups; IBA:GO_Central. DR GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway. DR Gene3D; 3.40.50.1000; -; 2. DR InterPro; IPR036412; HAD-like_sf. DR InterPro; IPR006379; HAD-SF_hydro_IIB. DR InterPro; IPR023214; HAD_sf. DR InterPro; IPR032710; NTF2-like_dom_sf. DR InterPro; IPR013679; SPP_C. DR InterPro; IPR006380; SPP_N. DR InterPro; IPR012847; Sucrose_phosphatase_pln/cyn. DR Pfam; PF05116; S6PP; 1. DR Pfam; PF08472; S6PP_C; 1. DR SUPFAM; SSF54427; SSF54427; 1. DR SUPFAM; SSF56784; SSF56784; 1. DR TIGRFAMs; TIGR01484; HAD-SF-IIB; 1. DR TIGRFAMs; TIGR01485; SPP_plant-cyano; 1. PE 2: Evidence at transcript level; KW Complete proteome; Hydrolase; Magnesium; Reference proteome. FT CHAIN 1 425 Probable sucrose-phosphatase 3a. FT /FTId=PRO_0000350615. SQ SEQUENCE 425 AA; 48644 MW; 7E8EFC939E5E1A3F CRC64; MDRLEGPPRL ILVADLDCTL VDHDDPENND LLRFNALWEA HYRHDSLLVY CTGRSFSSYS SLRKKRPLLT PDIAVTSVGS EIVYGGGEST VSDVVWTARL DYKWNRDIVV EETLKFPKLE PQPDKSQEEH KVSFFVGRED AVEIMKVLPG ILEERGVDVK LVYSNGYAFD VLPRGAGKQG ALTYLLDKLD IEGKQPSNTL VCGDSGNDAE LFNISDVYGV MVSNSHEELL QWYEENAKDN PKIFHASERC GAGMIEAIQR FNLGPNVSPR DVMDTENFHG ESLNPAHEVV QFYLFYERWR CGEVEKSDKY LQNLKSLSSP LGIFVHPSGV EKPIHEWIDE MENLYGDGKE KKFRIWLDNV TSSHISSDTW LAKFVKHELS EGKVRSCSTK VLLSYKEEKQ RLTWMHIHQS WLDESSSDDQ EKWIF //