ID CAS9_LISIN Reviewed; 1334 AA. AC Q927P4; DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2001, sequence version 1. DT 13-NOV-2013, entry version 60. DE RecName: Full=CRISPR-associated endonuclease Cas9; DE EC=3.1.-.-; GN Name=cas9; Synonyms=csn1; OrderedLocusNames=lin2744; OS Listeria innocua serovar 6a (strain CLIP 11262). OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria. OX NCBI_TaxID=272626; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CLIP 11262; RX PubMed=11679669; DOI=10.1126/science.1063447; RA Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., RA Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., RA Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., RA Domann E., Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., RA Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., RA Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., RA Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., RA Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., RA Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., RA Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., RA Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.; RT "Comparative genomics of Listeria species."; RL Science 294:849-852(2001). RN [2] RP FUNCTION AS AN DNA ENDONUCLEASE, SUBUNIT, AND RNA-BINDING. RC STRAIN=CLIP 11262; RX PubMed=22745249; DOI=10.1126/science.1225829; RA Jinek M., Chylinski K., Fonfara I., Hauer M., Doudna J.A., RA Charpentier E.; RT "A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial RT immunity."; RL Science 337:816-821(2012). CC -!- FUNCTION: CRISPR (clustered regularly interspaced short CC palindromic repeat) is an adaptive immune system that provides CC protection against mobile genetic elements (viruses, transposable CC elements and conjugative plasmids). CRISPR clusters contain CC spacers, sequences complementary to antecedent mobile elements, CC and target invading nucleic acids. CRISPR clusters are transcribed CC and processed into CRISPR RNA (crRNA). In type II CRISPR systems CC correct processing of pre-crRNA requires a trans-encoded small RNA CC (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein (By CC similarity). The tracrRNA serves as a guide for ribonuclease 3- CC aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA CC endonucleolytically cleaves linear or circular dsDNA target CC complementary to the spacer. CC -!- COFACTOR: Endonuclease activity on target dsDNA requires Mg(2+) CC (Probable). CC -!- SUBUNIT: Monomer (By similarity). Binds crRNA and tracrRNA. CC -!- DOMAIN: Has 2 endonuclease domains. The RuvC-like domain (in the CC N-terminus) cleaves the target DNA noncomplementary to crRNA while CC the HNH nuclease domain cleaves the target DNA complementary to CC crRNA (By similarity). CC -!- SIMILARITY: Belongs to the CRISPR-associated Cas9/Csn1 family. CC Subtype II/Nmemi subfamily. CC -!- SIMILARITY: Contains 1 HNH Cas9-type domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL596173; CAC97970.1; -; Genomic_DNA. DR PIR; AB1775; AB1775. DR RefSeq; NP_472073.1; NC_003212.1. DR ProteinModelPortal; Q927P4; -. DR STRING; 272626.lin2744; -. DR EnsemblBacteria; CAC97970; CAC97970; CAC97970. DR GeneID; 1131597; -. DR KEGG; lin:lin2744; -. DR PATRIC; 20302423; VBILisInn102668_2817. DR GenoList; LIN2744; -. DR HOGENOM; HOG000071789; -. DR KO; K09952; -. DR OMA; IPHQIHL; -. DR OrthoDB; EOG6MH57S; -. DR ProtClustDB; CLSK554964; -. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW. DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. DR HAMAP; MF_01480; Cas9; 1; -. DR InterPro; IPR010145; CRISPR-assoc_prot_SAG0894. DR InterPro; IPR025978; CRISPR-rel_HNH_dom. DR InterPro; IPR003615; HNH_nuc. DR Pfam; PF13395; HNH_4; 1. DR SMART; SM00507; HNHc; 1. DR TIGRFAMs; TIGR01865; cas_Csn1; 1. PE 1: Evidence at protein level; KW Antiviral defense; Complete proteome; DNA-binding; Endonuclease; KW Hydrolase; Magnesium; Nuclease; RNA-binding. FT CHAIN 1 1334 CRISPR-associated endonuclease Cas9. FT /FTId=PRO_0000421685. FT DOMAIN 824 874 HNH Cas9-type. SQ SEQUENCE 1334 AA; 154807 MW; 95557D51A4B7185E CRC64; MKKPYTIGLD IGTNSVGWAV LTDQYDLVKR KMKIAGDSEK KQIKKNFWGV RLFDEGQTAA DRRMARTARR RIERRRNRIS YLQGIFAEEM SKTDANFFCR LSDSFYVDNE KRNSRHPFFA TIEEEVEYHK NYPTIYHLRE ELVNSSEKAD LRLVYLALAH IIKYRGNFLI EGALDTQNTS VDGIYKQFIQ TYNQVFASGI EDGSLKKLED NKDVAKILVE KVTRKEKLER ILKLYPGEKS AGMFAQFISL IVGSKGNFQK PFDLIEKSDI ECAKDSYEED LESLLALIGD EYAELFVAAK NAYSAVVLSS IITVAETETN AKLSASMIER FDTHEEDLGE LKAFIKLHLP KHYEEIFSNT EKHGYAGYID GKTKQADFYK YMKMTLENIE GADYFIAKIE KENFLRKQRT FDNGAIPHQL HLEELEAILH QQAKYYPFLK ENYDKIKSLV TFRIPYFVGP LANGQSEFAW LTRKADGEIR PWNIEEKVDF GKSAVDFIEK MTNKDTYLPK ENVLPKHSLC YQKYLVYNEL TKVRYINDQG KTSYFSGQEK EQIFNDLFKQ KRKVKKKDLE LFLRNMSHVE SPTIEGLEDS FNSSYSTYHD LLKVGIKQEI LDNPVNTEML ENIVKILTVF EDKRMIKEQL QQFSDVLDGV VLKKLERRHY TGWGRLSAKL LMGIRDKQSH LTILDYLMND DGLNRNLMQL INDSNLSFKS IIEKEQVTTA DKDIQSIVAD LAGSPAIKKG ILQSLKIVDE LVSVMGYPPQ TIVVEMAREN QTTGKGKNNS RPRYKSLEKA IKEFGSQILK EHPTDNQELR NNRLYLYYLQ NGKDMYTGQD LDIHNLSNYD IDHIVPQSFI TDNSIDNLVL TSSAGNREKG DDVPPLEIVR KRKVFWEKLY QGNLMSKRKF DYLTKAERGG LTEADKARFI HRQLVETRQI TKNVANILHQ RFNYEKDDHG NTMKQVRIVT LKSALVSQFR KQFQLYKVRD VNDYHHAHDA YLNGVVANTL LKVYPQLEPE FVYGDYHQFD WFKANKATAK KQFYTNIMLF FAQKDRIIDE NGEILWDKKY LDTVKKVMSY RQMNIVKKTE IQKGEFSKAT IKPKGNSSKL IPRKTNWDPM KYGGLDSPNM AYAVVIEYAK GKNKLVFEKK IIRVTIMERK AFEKDEKAFL EEQGYRQPKV LAKLPKYTLY ECEEGRRRML ASANEAQKGN QQVLPNHLVT LLHHAANCEV SDGKSLDYIE SNREMFAELL AHVSEFAKRY TLAEANLNKI NQLFEQNKEG DIKAIAQSFV DLMAFNAMGA PASFKFFETT IERKRYNNLK ELLNSTIIYQ SITGLYESRK RLDD //