ID NCB2_YEAST Reviewed; 146 AA. AC Q92317; O00064; P87340; Q04176; DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1997, sequence version 1. DT 23-JAN-2007, entry version 46. DE Negative cofactor 2 complex subunit beta (NC2 complex subunit beta) DE (Transcriptional repressor YDR1). GN Name=NCB2; Synonyms=YDR1; OrderedLocusNames=YDR397C; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN THE NC2 COMPLEX, RP AND FUNCTION OF THE NC2 COMPLEX. RX MEDLINE=97175685; PubMed=9023340; DOI=10.1073/pnas.94.3.820; RA Kim S., Na J.G., Hampsey M., Reinberg D.; RT "The Dr1/DRAP1 heterodimer is a global repressor of transcription in RT vivo."; RL Proc. Natl. Acad. Sci. U.S.A. 94:820-825(1997). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, AND FUNCTION OF THE NC2 COMPLEX. RX MEDLINE=97105886; PubMed=8948634; DOI=10.1093/nar/24.22.4450; RA Goppelt A.R., Meisterernst M.; RT "Characterization of the basal inhibitor of class II transcription NC2 RT from Saccharomyces cerevisiae."; RL Nucleic Acids Res. 24:4450-4455(1996). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=97313263; PubMed=9169867; RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., RA Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., RA Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., RA Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., RA Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., RA Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., RA Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., RA Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., RA Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., RA Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., RA Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., RA Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., RA Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., RA Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., RA Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., RA Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., RA Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., RA Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., RA Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., RA Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., RA Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., RA Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., RA Waterston R., Albermann K., Hani J., Heumann K., Kleine K., RA Mewes H.-W., Zollner A., Zaccaria P.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."; RL Nature 387:75-78(1997). RN [4] RP SUBUNIT, FUNCTION OF THE NC2 COMPLEX, AND INTERACTION WITH SPT15. RX PubMed=9096360; DOI=10.1073/pnas.94.7.3145; RA Gadbois E.L., Chao D.M., Reese J.C., Green M.R., Young R.A.; RT "Functional antagonism between RNA polymerase II holoenzyme and global RT negative regulator NC2 in vivo."; RL Proc. Natl. Acad. Sci. U.S.A. 94:3145-3150(1997). RN [5] RP FUNCTION OF THE NC2 COMPLEX IN TRANSCRIPTIONAL ACTIVATION. RX PubMed=12237409; DOI=10.1073/pnas.202236699; RA Cang Y., Prelich G.; RT "Direct stimulation of transcription by negative cofactor 2 (NC2) RT through TATA-binding protein (TBP)."; RL Proc. Natl. Acad. Sci. U.S.A. 99:12727-12732(2002). RN [6] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [7] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). CC -!- FUNCTION: Component of the NC2 complex which represses RNA CC polymerase II transcription through binding to SPT15/TBP and CC thereby inhibiting the assembly of the preinitiation complex. The CC NC2 complex may also mediate transcriptional activation from TATA- CC driven promoters through association with SPT15/TBP. CC -!- SUBUNIT: Component of the NC2 (negative cofactor 2) complex CC composed of BUR6 and NCB2. The NC2 complex associates with CC SPT15/TBP. Interacts with SPT15/TBP. CC -!- INTERACTION: CC P13393:SPT15; NbExp=1; IntAct=EBI-37723, EBI-19129; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- MISCELLANEOUS: Present with 2950 molecules/cell. CC -!- CAUTION: Ref.3 (AAB64838) sequence differs from that shown due to CC erroneous gene model prediction. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U91948; AAB51375.1; -; Genomic_DNA. DR EMBL; Y09266; CAA70461.1; -; mRNA. DR EMBL; U32274; AAB64838.1; ALT_SEQ; Genomic_DNA. DR PIR; S69694; S69694. DR HSSP; Q01658; 1JFI. DR DIP; DIP:2067N; -. DR IntAct; Q92317; -. DR TRANSFAC; T03512; -. DR Ensembl; YDR397C; Saccharomyces cerevisiae. DR GenomeReviews; Z71256_GR; YDR397C. DR KEGG; sce:YDR397C; -. DR SGD; S000002805; NCB2. DR LinkHub; Q92317; -. DR GermOnline; YDR397C; Saccharomyces cerevisiae. DR GO; GO:0005634; C:nucleus; IPI:SGD. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0003714; F:transcription corepressor activity; IGI:SGD. DR GO; GO:0000122; P:negative regulation of transcription from R...; IGI:SGD. DR InterPro; IPR003958; CBFA_NFYB_domain. DR InterPro; IPR009072; Histone-fold. DR Gene3D; G3DSA:1.10.20.10; Histone-fold; 1. DR Pfam; PF00808; CBFD_NFYB_HMF; 1. KW Activator; Complete proteome; Nuclear protein; Repressor; KW Transcription; Transcription regulation. FT CHAIN 1 146 Negative cofactor 2 complex subunit beta. FT /FTId=PRO_0000255268. SQ SEQUENCE 146 AA; 16650 MW; E04EB0D6F7E8D91D CRC64; MAGDSDNVSL PKATVQKMIS EILDQDLMFT KDAREIIINS GIEFIMILSS MASEMADNEA KKTIAPEHVI KALEELEYNE FIPFLEEILL NFKGSQKVKE TRDSKFKKSG LSEEELLRQQ EELFRQSRSR LHHNSVSDPV KSEDSS //