ID NCB2_YEAST Reviewed; 146 AA. AC Q92317; D6VT30; O00064; P87340; Q04176; DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1997, sequence version 1. DT 01-MAY-2013, entry version 109. DE RecName: Full=Negative cofactor 2 complex subunit beta; DE Short=NC2 complex subunit beta; DE AltName: Full=Transcriptional repressor YDR1; GN Name=NCB2; Synonyms=YDR1; OrderedLocusNames=YDR397C; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN THE NC2 COMPLEX, RP AND FUNCTION OF THE NC2 COMPLEX. RX PubMed=9023340; DOI=10.1073/pnas.94.3.820; RA Kim S., Na J.G., Hampsey M., Reinberg D.; RT "The Dr1/DRAP1 heterodimer is a global repressor of transcription in RT vivo."; RL Proc. Natl. Acad. Sci. U.S.A. 94:820-825(1997). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, AND FUNCTION OF THE NC2 COMPLEX. RX PubMed=8948634; DOI=10.1093/nar/24.22.4450; RA Goppelt A.R., Meisterernst M.; RT "Characterization of the basal inhibitor of class II transcription NC2 RT from Saccharomyces cerevisiae."; RL Nucleic Acids Res. 24:4450-4455(1996). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=9169867; RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., RA Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., RA Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., RA Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., RA Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., RA Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., RA Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., RA Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., RA Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., RA Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., RA Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., RA Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., RA Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., RA Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., RA Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., RA Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., RA Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., RA Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., RA Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., RA Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., RA Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., RA Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., RA Waterston R., Albermann K., Hani J., Heumann K., Kleine K., RA Mewes H.-W., Zollner A., Zaccaria P.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."; RL Nature 387:75-78(1997). RN [4] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RG Saccharomyces Genome Database; RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases. RN [5] RP SUBUNIT, FUNCTION OF THE NC2 COMPLEX, AND INTERACTION WITH SPT15. RX PubMed=9096360; DOI=10.1073/pnas.94.7.3145; RA Gadbois E.L., Chao D.M., Reese J.C., Green M.R., Young R.A.; RT "Functional antagonism between RNA polymerase II holoenzyme and global RT negative regulator NC2 in vivo."; RL Proc. Natl. Acad. Sci. U.S.A. 94:3145-3150(1997). RN [6] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135 AND SER-145, AND RP MASS SPECTROMETRY. RC STRAIN=2124; RX PubMed=11875433; DOI=10.1038/nbt0302-301; RA Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., RA Shabanowitz J., Hunt D.F., White F.M.; RT "Phosphoproteome analysis by mass spectrometry and its application to RT Saccharomyces cerevisiae."; RL Nat. Biotechnol. 20:301-305(2002). RN [7] RP FUNCTION OF THE NC2 COMPLEX IN TRANSCRIPTIONAL ACTIVATION. RX PubMed=12237409; DOI=10.1073/pnas.202236699; RA Cang Y., Prelich G.; RT "Direct stimulation of transcription by negative cofactor 2 (NC2) RT through TATA-binding protein (TBP)."; RL Proc. Natl. Acad. Sci. U.S.A. 99:12727-12732(2002). RN [8] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [9] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [10] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135; SER-137 AND RP SER-142, AND MASS SPECTROMETRY. RC STRAIN=ADR376; RX PubMed=17330950; DOI=10.1021/pr060559j; RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., RA Elias J.E., Gygi S.P.; RT "Large-scale phosphorylation analysis of alpha-factor-arrested RT Saccharomyces cerevisiae."; RL J. Proteome Res. 6:1190-1197(2007). RN [11] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-145, AND MASS RP SPECTROMETRY. RX PubMed=17563356; DOI=10.1073/pnas.0701622104; RA Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; RT "Proteome-wide identification of in vivo targets of DNA damage RT checkpoint kinases."; RL Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). RN [12] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112; SER-135; SER-142; RP SER-145 AND SER-146, AND MASS SPECTROMETRY. RX PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200; RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; RT "A multidimensional chromatography technology for in-depth RT phosphoproteome analysis."; RL Mol. Cell. Proteomics 7:1389-1396(2008). CC -!- FUNCTION: Component of the NC2 complex which represses RNA CC polymerase II transcription through binding to SPT15/TBP and CC thereby inhibiting the assembly of the preinitiation complex. The CC NC2 complex may also mediate transcriptional activation from TATA- CC driven promoters through association with SPT15/TBP. CC -!- SUBUNIT: Component of the NC2 (negative cofactor 2) complex CC composed of BUR6 and NCB2. The NC2 complex associates with CC SPT15/TBP. Interacts with SPT15/TBP. CC -!- INTERACTION: CC P40096:BUR6; NbExp=4; IntAct=EBI-37723, EBI-11908; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- MISCELLANEOUS: Present with 2950 molecules/cell in log phase SD CC medium. CC -!- SEQUENCE CAUTION: CC Sequence=AAB64838.1; Type=Erroneous gene model prediction; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U91948; AAB51375.1; -; Genomic_DNA. DR EMBL; Y09266; CAA70461.1; -; mRNA. DR EMBL; U32274; AAB64838.1; ALT_SEQ; Genomic_DNA. DR EMBL; BK006938; DAA12240.1; -; Genomic_DNA. DR PIR; S69694; S69694. DR RefSeq; NP_010685.1; NM_001180705.1. DR HSSP; Q01658; 1JFI. DR ProteinModelPortal; Q92317; -. DR SMR; Q92317; 6-128. DR DIP; DIP-2067N; -. DR IntAct; Q92317; 6. DR MINT; MINT-528090; -. DR PaxDb; Q92317; -. DR EnsemblFungi; YDR397C; YDR397C; YDR397C. DR GeneID; 852006; -. DR KEGG; sce:YDR397C; -. DR CYGD; YDR397c; -. DR SGD; S000002805; NCB2. DR eggNOG; COG5150; -. DR GeneTree; ENSGT00550000075010; -. DR HOGENOM; HOG000178641; -. DR OrthoDB; EOG41NXWS; -. DR NextBio; 970198; -. DR Genevestigator; Q92317; -. DR GermOnline; YDR397C; Saccharomyces cerevisiae. DR GO; GO:0017054; C:negative cofactor 2 complex; IDA:SGD. DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro. DR GO; GO:0003713; F:transcription coactivator activity; IDA:SGD. DR GO; GO:0003714; F:transcription corepressor activity; IMP:SGD. DR GO; GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD. DR GO; GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:SGD. DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. DR Gene3D; 1.10.20.10; -; 1. DR InterPro; IPR003958; CBFA_NFYB_domain. DR InterPro; IPR027113; Dr1/Ncb2. DR InterPro; IPR009072; Histone-fold. DR PANTHER; PTHR11064:SF8; PTHR11064:SF8; 1. DR Pfam; PF00808; CBFD_NFYB_HMF; 1. DR SUPFAM; SSF47113; Histone-fold; 1. PE 1: Evidence at protein level; KW Activator; Complete proteome; Nucleus; Phosphoprotein; KW Reference proteome; Repressor; Transcription; KW Transcription regulation. FT CHAIN 1 146 Negative cofactor 2 complex subunit beta. FT /FTId=PRO_0000255268. FT MOD_RES 112 112 Phosphoserine. FT MOD_RES 135 135 Phosphoserine. FT MOD_RES 137 137 Phosphoserine. FT MOD_RES 142 142 Phosphoserine. FT MOD_RES 145 145 Phosphoserine. FT MOD_RES 146 146 Phosphoserine. SQ SEQUENCE 146 AA; 16650 MW; E04EB0D6F7E8D91D CRC64; MAGDSDNVSL PKATVQKMIS EILDQDLMFT KDAREIIINS GIEFIMILSS MASEMADNEA KKTIAPEHVI KALEELEYNE FIPFLEEILL NFKGSQKVKE TRDSKFKKSG LSEEELLRQQ EELFRQSRSR LHHNSVSDPV KSEDSS //