ID 5MP1_MOUSE Reviewed; 419 AA. AC Q91VK1; Q9D0N4; DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2001, sequence version 1. DT 24-JUL-2024, entry version 141. DE RecName: Full=eIF5-mimic protein 1 {ECO:0000250|UniProtKB:Q9Y6E2}; DE AltName: Full=Basic leucine zipper and W2 domain-containing protein 2; GN Name=Bzw2; Synonyms=5mp1 {ECO:0000250|UniProtKB:Q9Y6E2}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=C57BL/6J; TISSUE=Embryo; RX PubMed=16141072; DOI=10.1126/science.1112014; RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.; RT "The transcriptional landscape of the mammalian genome."; RL Science 309:1559-1563(2005). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=FVB/N; TISSUE=Mammary tumor; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [3] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Embryonic brain; RX PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200; RA Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.; RT "Phosphoproteomic analysis of the developing mouse brain."; RL Mol. Cell. Proteomics 3:1093-1101(2004). RN [4] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412, AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Brown adipose tissue, Heart, Kidney, Lung, Pancreas, and Spleen; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). CC -!- FUNCTION: Translation initiation regulator which represses non-AUG CC initiated translation and repeat-associated non-AUG (RAN) initiated CC translation by acting as a competitive inhibitor of eukaryotic CC translation initiation factor 5 (EIF5) function (By similarity). CC Increases the accuracy of translation initiation by impeding EIF5- CC dependent translation from non-AUG codons by competing with it for CC interaction with EIF2S2 within the 43S pre-initiation complex (PIC) in CC an EIF3C-binding dependent manner (By similarity). CC {ECO:0000250|UniProtKB:Q9Y6E2}. CC -!- SUBUNIT: Interacts with EIF3E, EIF2S2 and EIF3C. CC {ECO:0000250|UniProtKB:Q9Y6E2}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9Y6E2}. CC -!- SIMILARITY: Belongs to the BZW family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK011253; BAB27495.1; -; mRNA. DR EMBL; AK160544; BAE35861.1; -; mRNA. DR EMBL; BC013060; AAH13060.1; -; mRNA. DR CCDS; CCDS25884.1; -. DR RefSeq; NP_080116.2; NM_025840.3. DR AlphaFoldDB; Q91VK1; -. DR SMR; Q91VK1; -. DR BioGRID; 211804; 9. DR IntAct; Q91VK1; 4. DR MINT; Q91VK1; -. DR STRING; 10090.ENSMUSP00000020856; -. DR iPTMnet; Q91VK1; -. DR PhosphoSitePlus; Q91VK1; -. DR SwissPalm; Q91VK1; -. DR jPOST; Q91VK1; -. DR PaxDb; 10090-ENSMUSP00000020856; -. DR PeptideAtlas; Q91VK1; -. DR ProteomicsDB; 273781; -. DR Pumba; Q91VK1; -. DR Antibodypedia; 11817; 113 antibodies from 26 providers. DR DNASU; 66912; -. DR Ensembl; ENSMUST00000020856.6; ENSMUSP00000020856.5; ENSMUSG00000020547.6. DR GeneID; 66912; -. DR KEGG; mmu:66912; -. DR UCSC; uc007njp.2; mouse. DR AGR; MGI:1914162; -. DR CTD; 28969; -. DR MGI; MGI:1914162; Bzw2. DR VEuPathDB; HostDB:ENSMUSG00000020547; -. DR eggNOG; KOG2297; Eukaryota. DR GeneTree; ENSGT00390000012561; -. DR HOGENOM; CLU_032849_0_1_1; -. DR InParanoid; Q91VK1; -. DR OMA; ELIQCIW; -. DR OrthoDB; 5475947at2759; -. DR PhylomeDB; Q91VK1; -. DR TreeFam; TF324313; -. DR BioGRID-ORCS; 66912; 5 hits in 78 CRISPR screens. DR ChiTaRS; Bzw2; mouse. DR PRO; PR:Q91VK1; -. DR Proteomes; UP000000589; Chromosome 12. DR RNAct; Q91VK1; Protein. DR Bgee; ENSMUSG00000020547; Expressed in cortical plate and 268 other cell types or tissues. DR ExpressionAtlas; Q91VK1; baseline and differential. DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB. DR GO; GO:0006446; P:regulation of translational initiation; ISS:UniProtKB. DR CDD; cd11560; W2_eIF5C_like; 1. DR Gene3D; 1.25.40.180; -; 1. DR InterPro; IPR016024; ARM-type_fold. DR InterPro; IPR051245; eIF5-mimic_regulator. DR InterPro; IPR043510; W2_BZW1/2. DR InterPro; IPR003307; W2_domain. DR PANTHER; PTHR14208; BASIC LEUCINE ZIPPER AND W2 DOMAIN-CONTAINING PROTEIN; 1. DR PANTHER; PTHR14208:SF7; EIF5-MIMIC PROTEIN 1; 1. DR Pfam; PF02020; W2; 1. DR SMART; SM00515; eIF5C; 1. DR SUPFAM; SSF48371; ARM repeat; 1. DR PROSITE; PS51363; W2; 1. PE 1: Evidence at protein level; KW Acetylation; Cytoplasm; Phosphoprotein; Reference proteome; KW Translation regulation. FT CHAIN 1..419 FT /note="eIF5-mimic protein 1" FT /id="PRO_0000254620" FT DOMAIN 248..415 FT /note="W2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00695" FT REGION 1..22 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 117 FT /note="N6-acetyllysine" FT /evidence="ECO:0000250|UniProtKB:Q9Y6E2" FT MOD_RES 412 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:21183079" FT MOD_RES 414 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:Q9Y6E2" FT MOD_RES 419 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:Q9WTT7" FT CONFLICT 69 FT /note="F -> L (in Ref. 1; BAB27495)" FT /evidence="ECO:0000305" FT CONFLICT 326 FT /note="A -> R (in Ref. 1; BAB27495)" FT /evidence="ECO:0000305" SQ SEQUENCE 419 AA; 48063 MW; 996FC462B50EAF6F CRC64; MNKHQKPVLT GQRFKTRKRD EKEKFEPTVF RDTLVQGLNE AGDDLEAVAK FLDSTGSRLD YRRYADTLFD ILVAGSMLAP GGTRIDDGDK TKMTNHCVFS ANEDHETIRN YAQVFNKLIR RYKYLEKAFE DEMKKLLLFL KAFSEAEQTK LAMLSGILLG NGTLPATILT SLFTDSLVKE GIAASFAVKL FKAWMAEKDA NSVTSSLRKA NLDKRLLELF PVNRQSVDHF AKYFTDAGLK ELSDFLRVQQ SLGTRKELQK ELQERLSQEC PIKEVVLYVK EEMKRNDLPE TAVIGLLWTC IMNAVEWNKK EELVAEQALK HLKQYAPLLA VFSSQGQSEL VLLQKVQEYC YDNIHFMKAF QKIVVLFYKA DVLSEEAILK WYKEAHAAKG KSVFLDQMKK FVEWLQNAEE ESESEGEES //