ID Q90VU7_9HIV1 Unreviewed; 206 AA. AC Q90VU7; DT 01-DEC-2001, integrated into UniProtKB/TrEMBL. DT 01-DEC-2001, sequence version 1. DT 17-FEB-2016, entry version 90. DE RecName: Full=Protein Nef {ECO:0000256|RuleBase:RU000344, ECO:0000256|SAAS:SAAS00004511}; GN Name=nef {ECO:0000313|EMBL:AAB60579.1}; OS Human immunodeficiency virus 1. OC Viruses; Retro-transcribing viruses; Retroviridae; Orthoretrovirinae; OC Lentivirus; Primate lentivirus group. OX NCBI_TaxID=11676 {ECO:0000313|EMBL:AAB60579.1}; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] {ECO:0000313|EMBL:AAB60579.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=NL4-3 {ECO:0000313|EMBL:AAB60579.1}; RX PubMed=3016298; RA Adachi A., Gendelman H.E., Koenig S., Folks T., Willey R., Rabson A., RA Martin M.A.; RT "Production of acquired immunodeficiency syndrome-associated RT retrovirus in human and nonhuman cells transfected with an infectious RT molecular clone."; RL J. Virol. 59:284-291(1986). RN [2] {ECO:0000313|PIR:JQ1620} RP NUCLEOTIDE SEQUENCE. RX PubMed=1629704; RA Smith J., Azad A., Deacon N.; RT "Identification of two novel human immunodeficiency virus type 1 RT splice acceptor sites in infected T cell lines."; RL J. Gen. Virol. 73:1825-1828(1992). RN [3] {ECO:0000313|PIR:S43467} RP PROTEIN SEQUENCE. RX PubMed=8174561; DOI=10.1111/j.1432-1033.1994.tb18795.x; RA Freund J., Kellner R., Houthaeve T., Kalbitzer H.R.; RT "Stability and proteolytic domains of Nef protein from human RT immunodeficiency virus (HIV) type 1."; RL Eur. J. Biochem. 221:811-819(1994). RN [4] {ECO:0000313|EMBL:AAB60579.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=NL4-3 {ECO:0000313|EMBL:AAB60579.1}; RX PubMed=7483282; DOI=10.1006/viro.1995.1548; RA Salminen M.O., Koch C., Sanders-Buell E., Ehrenberg P.K., RA Michael N.L., Carr J.K., Burke D.S., McCutchan F.E.; RT "Recovery of virtually full-length HIV-1 provirus of diverse subtypes RT from primary virus cultures using the polymerase chain reaction."; RL Virology 213:80-86(1995). RN [5] {ECO:0000313|EMBL:AAB60579.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=NL4-3 {ECO:0000313|EMBL:AAB60579.1}; RA Salminen M.S.; RL Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases. RN [6] {ECO:0000313|PIR:JC5400} RP PROTEIN SEQUENCE. RX PubMed=9126340; DOI=10.1006/bbrc.1997.6349; RA Macreadie I.G., Lowe M.G., Curtain C.C., Hewish D., Azad A.A.; RT "Cytotoxicity resulting from addition of HIV-1 Nef N-terminal peptides RT to yeast and bacterial cells."; RL Biochem. Biophys. Res. Commun. 232:707-711(1997). RN [7] {ECO:0000313|EMBL:CAH64567.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=16032733; DOI=10.1002/jmv.20408; RA Parreira R., Padua E., Piedade J., Venenno T., Paixao M.T., RA Esteves A.; RT "Genetic analysis of human immunodeficiency virus type 1 nef in RT Portugal: subtyping, identification of mosaic genes, and amino acid RT sequence variability."; RL J. Med. Virol. 77:8-16(2005). RN [8] {ECO:0000313|EMBL:ABY49078.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=184003/B8 {ECO:0000313|EMBL:ABY49078.1}; RX PubMed=18184702; DOI=10.1128/JVI.02260-07; RA Turk G., Gherardi M.M., Laufer N., Saracco M., Luzzi R., Cox J.H., RA Cahn P., Salomon H.; RT "Magnitude, breadth, and functional profile of T-cell responses during RT human immunodeficiency virus primary infection with B and BF viral RT variants."; RL J. Virol. 82:2853-2866(2008). RN [9] {ECO:0000313|EMBL:ACM50127.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=F781 {ECO:0000313|EMBL:ACM50127.1}; RX PubMed=19036810; DOI=10.1128/JVI.01061-08; RA Wang Y.E., Li B., Carlson J.M., Streeck H., Gladden A.D., Goodman R., RA Schneidewind A., Power K.A., Toth I., Frahm N., Alter G., Brander C., RA Carrington M., Walker B.D., Altfeld M., Heckerman D., Allen T.M.; RT "Protective HLA class I alleles that restrict acute-phase CD8+ T-cell RT responses are associated with viral escape mutations located in highly RT conserved regions of human immunodeficiency virus type 1."; RL J. Virol. 83:1845-1855(2009). RN [10] {ECO:0000313|EMBL:ADB03681.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=BEC73 {ECO:0000313|EMBL:ADB03681.1}; RA Miura T., Brumme C.J., Brockman M.A., Brumme Z.L., Pereyra F., RA Block B.L., Trocha A., John M., Mallal S., Harrigan P.R., Walker B.D.; RT "HLA-associated viral mutations are common in Human immunodeficiency RT virus type 1 elite controllers."; RL J. Virol. 84:1212-1212(2010). RN [11] {ECO:0000313|EMBL:ADJ17496.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=5569.6 {ECO:0000313|EMBL:ADJ17498.1}, 5569.7 RC {ECO:0000313|EMBL:ADJ17499.1}, 5569.81 {ECO:0000313|EMBL:ADJ17500.1}, RC 5569.82 {ECO:0000313|EMBL:ADJ17501.1}, 5569.83 RC {ECO:0000313|EMBL:ADJ17502.1}, and 5569.at RC {ECO:0000313|EMBL:ADJ17496.1}; RX PubMed=20463068; DOI=10.1128/JVI.00619-10; RA Specht A., Telenti A., Martinez R., Fellay J., Bailes E., Evans D.T., RA Carrington M., Hahn B.H., Goldstein D.B., Kirchhoff F.; RT "Counteraction of HLA-C-mediated immune control of HIV-1 by Nef."; RL J. Virol. 84:7300-7311(2010). RN [12] {ECO:0000213|PDB:4EMZ, ECO:0000213|PDB:4EN2} RP X-RAY CRYSTALLOGRAPHY (2.58 ANGSTROMS). RX PubMed=22705789; DOI=10.1038/nsmb.2328; RA Jia X., Singh R., Homann S., Yang H., Guatelli J., Xiong Y.; RT "Structural basis of evasion of cellular adaptive immunity by HIV-1 RT Nef."; RL Nat. Struct. Mol. Biol. 19:701-706(2012). RN [13] {ECO:0000313|EMBL:AFM44079.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=HIV/US/BID-V4141 {ECO:0000313|EMBL:AFM44079.1}; RX PubMed=22412369; DOI=10.1371/journal.ppat.1002529; RA Henn M.R., Boutwell C.L., Charlebois P., Lennon N.J., Power K.A., RA Macalalad A.R., Berlin A.M., Malboeuf C.M., Ryan E.M., Gnerre S., RA Zody M.C., Erlich R.L., Green L.M., Berical A., Wang Y., Casali M., RA Streeck H., Bloom A.K., Dudek T., Tully D., Newman R., Axten K.L., RA Gladden A.D., Battis L., Kemper M., Zeng Q., Shea T.P., Gujja S., RA Zedlack C., Gasser O., Brander C., Hess C., Gunthard H.F., RA Brumme Z.L., Brumme C.J., Bazner S., Rychert J., Tinsley J.P., RA Mayer K.H., Rosenberg E., Pereyra F., Levin J.Z., Young S.K., RA Jessen H., Altfeld M., Birren B.W., Walker B.D., Allen T.M.; RT "Whole genome deep sequencing of HIV-1 reveals the impact of early RT minor variants upon immune recognition during acute infection."; RL PLoS Pathog. 8:E1002529-E1002529(2012). RN [14] {ECO:0000313|EMBL:AGV32938.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=16CB1_20F5 {ECO:0000313|EMBL:AGV32938.1}, 16CB1_20F7 RC {ECO:0000313|EMBL:AGV32939.1}, and 22CB6_23G3 RC {ECO:0000313|EMBL:AGV33378.1}; RA Ho Y.-C., Shan L., Hosmane N.N., Wang J., Laskey S.B., RA Rosenbloom D.I., Lai J., Blankson J.N., Siliciano R.F.; RT "Replication-competent non-induced proviruses in the latent reservoir RT increase barrier to HIV-1 cure."; RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases. RN [15] {ECO:0000313|EMBL:AIW80604.1} RP NUCLEOTIDE SEQUENCE. RA Whitcomb J.; RT "Reference sequence used by Monogram Biosciences for genotyping assays RT of HIV."; RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases. RN [16] {ECO:0000313|EMBL:AKZ17633.1} RP NUCLEOTIDE SEQUENCE. RA Singh J., Ronsard L., Ramachandran V.G., Banerjea A.C.; RT "Genetic and functional characterization of HIV-1 nef gene."; RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases. CC -!- SIMILARITY: Belongs to the lentivirus primate group Nef protein CC family. {ECO:0000256|RuleBase:RU000344}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U26942; AAB60579.1; -; Genomic_DNA. DR EMBL; EU312175; ABY49078.1; -; Genomic_DNA. DR EMBL; FJ469736; ACM50127.1; -; Genomic_DNA. DR EMBL; GU046594; ADB03681.1; -; Genomic_DNA. DR EMBL; HM244488; ADJ17496.1; -; Genomic_RNA. DR EMBL; HM244490; ADJ17498.1; -; Genomic_RNA. DR EMBL; HM244491; ADJ17499.1; -; Genomic_RNA. DR EMBL; HM244492; ADJ17500.1; -; Genomic_RNA. DR EMBL; HM244493; ADJ17501.1; -; Genomic_RNA. DR EMBL; HM244494; ADJ17502.1; -; Genomic_RNA. DR EMBL; JQ403040; AFM44079.1; -; Genomic_RNA. DR EMBL; KF526172; AGV32938.1; -; Genomic_DNA. DR EMBL; KF526173; AGV32939.1; -; Genomic_DNA. DR EMBL; KF526308; AGV33378.1; -; Genomic_DNA. DR EMBL; KM390026; AIW80604.1; -; Viral_cRNA. DR EMBL; KP851719; AKR15819.1; -; Genomic_DNA. DR EMBL; KP851720; AKR15820.1; -; Genomic_DNA. DR EMBL; KP851721; AKR15821.1; -; Genomic_DNA. DR EMBL; KP851722; AKR15822.1; -; Genomic_DNA. DR EMBL; KP851723; AKR15823.1; -; Genomic_DNA. DR EMBL; KP851724; AKR15824.1; -; Genomic_DNA. DR EMBL; KP851725; AKR15825.1; -; Genomic_DNA. DR EMBL; KP851726; AKR15826.1; -; Genomic_DNA. DR EMBL; KP851727; AKR15827.1; -; Genomic_DNA. DR EMBL; KP851728; AKR15828.1; -; Genomic_DNA. DR EMBL; KP851729; AKR15829.1; -; Genomic_DNA. DR EMBL; KP851730; AKR15830.1; -; Genomic_DNA. DR EMBL; KP851731; AKR15831.1; -; Genomic_DNA. DR EMBL; KP851732; AKR15832.1; -; Genomic_DNA. DR EMBL; KP851733; AKR15833.1; -; Genomic_DNA. DR EMBL; KP851734; AKR15834.1; -; Genomic_DNA. DR EMBL; KP851735; AKR15835.1; -; Genomic_DNA. DR EMBL; KP851736; AKR15836.1; -; Genomic_DNA. DR EMBL; KP851737; AKR15837.1; -; Genomic_DNA. DR EMBL; KP851738; AKR15838.1; -; Genomic_DNA. DR EMBL; KP851739; AKR15839.1; -; Genomic_DNA. DR EMBL; KP851740; AKR15840.1; -; Genomic_DNA. DR EMBL; KP851741; AKR15841.1; -; Genomic_DNA. DR EMBL; KP851742; AKR15842.1; -; Genomic_DNA. DR EMBL; KR818790; AKZ17633.1; -; Genomic_DNA. DR EMBL; KR818792; AKZ17634.1; -; Genomic_DNA. DR EMBL; AJ850868; CAH64567.1; -; Genomic_DNA. DR PIR; JC5400; JC5400. DR PIR; JQ1620; JQ1620. DR PIR; S03244; S03244. DR PIR; S43467; S43467. DR PDB; 4EMZ; X-ray; 2.90 A; B/C=1-206. DR PDB; 4EN2; X-ray; 2.58 A; B/C=1-206. DR PDBsum; 4EMZ; -. DR PDBsum; 4EN2; -. DR DIP; DIP-43762N; -. DR MINT; MINT-2791815; -. DR MINT; MINT-5281653; -. DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-KW. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0051117; F:ATPase binding; ISS:UniProtKB. DR GO; GO:0005516; F:calmodulin binding; ISS:UniProtKB. DR GO; GO:0042609; F:CD4 receptor binding; ISS:UniProtKB. DR GO; GO:0005525; F:GTP binding; IEA:InterPro. DR GO; GO:0042288; F:MHC class I protein binding; ISS:UniProtKB. DR GO; GO:0019901; F:protein kinase binding; ISS:UniProtKB. DR GO; GO:0005102; F:receptor binding; ISS:UniProtKB. DR GO; GO:0017124; F:SH3 domain binding; ISS:UniProtKB. DR GO; GO:0031996; F:thioesterase binding; ISS:UniProtKB. DR GO; GO:0052085; P:negative regulation by symbiont of host T-cell mediated immune response; ISS:UniProtKB. DR GO; GO:0045225; P:negative regulation of CD4 biosynthetic process; ISS:UniProtKB. DR GO; GO:0009405; P:pathogenesis; ISS:UniProtKB. DR GO; GO:0050848; P:regulation of calcium-mediated signaling; ISS:UniProtKB. DR GO; GO:0046776; P:suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I; ISS:UniProtKB. DR GO; GO:0019058; P:viral life cycle; ISS:UniProtKB. DR Gene3D; 3.30.62.10; -; 1. DR Gene3D; 4.10.890.10; -; 1. DR InterPro; IPR027480; HIV-1_Nef_anchor. DR InterPro; IPR027481; HIV-1_Nef_core. DR InterPro; IPR001558; HIV_Nef. DR Pfam; PF00469; F-protein; 1. DR SUPFAM; SSF55671; SSF55671; 1. PE 1: Evidence at protein level; KW 3D-structure {ECO:0000213|PDB:4EMZ, ECO:0000213|PDB:4EN2}; KW Host cell membrane {ECO:0000256|SAAS:SAAS00417561}; KW Host membrane {ECO:0000256|SAAS:SAAS00417568}; KW Host-virus interaction {ECO:0000256|SAAS:SAAS00417537}; KW Lipoprotein {ECO:0000256|RuleBase:RU003609}; KW Membrane {ECO:0000256|SAAS:SAAS00417549}; KW Myristate {ECO:0000256|RuleBase:RU003609}; KW Viral immunoevasion {ECO:0000256|RuleBase:RU000344}; KW Virulence {ECO:0000256|RuleBase:RU000344}. SQ SEQUENCE 206 AA; 23367 MW; 65AF3B6184DC2FE7 CRC64; MGGKWSKSSV IGWPAVRERM RRAEPAADGV GAVSRDLEKH GAITSSNTAA NNAACAWLEA QEEEEVGFPV TPQVPLRPMT YKAAVDLSHF LKEKGGLEGL IHSQRRQDIL DLWIYHTQGY FPDWQNYTPG PGVRYPLTFG WCYKLVPVEP DKVEEANKGE NTSLLHPVSL HGMDDPEREV LEWRFDSRLA FHHVARELHP EYFKNC //