ID Q90VU7_9HIV1 Unreviewed; 206 AA. AC Q90VU7; DT 01-DEC-2001, integrated into UniProtKB/TrEMBL. DT 01-DEC-2001, sequence version 1. DT 03-APR-2007, entry version 27. DE NEF protein (Negative factor). GN Name=nef; OS Human immunodeficiency virus 1. OC Viruses; Retro-transcribing viruses; Retroviridae; Orthoretrovirinae; OC Lentivirus; Primate lentivirus group. OX NCBI_TaxID=11676; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=NL4-3; RX MEDLINE=96036482; PubMed=7483282; RA Salminen M.O., Koch C., Sanders-Buell E., Ehrenberg P.K., RA Michael N.L., Carr J.K., Burke D.S., McCutchan F.E.; RT "Recovery of virtually full-length HIV-1 provirus of diverse subtypes RT from primary virus cultures using the polymerase chain reaction."; RL Virology 213:80-86(1995). RN [2] RP NUCLEOTIDE SEQUENCE. RC STRAIN=NL4-3; RX MEDLINE=86281827; PubMed=3016298; RA Adachi A., Gendelman H.E., Koenig S., Folks T., Willey R., Rabson A., RA Martin M.A.; RT "Production of acquired immunodeficiency syndrome-associated RT retrovirus in human and nonhuman cells transfected with an infectious RT molecular clone."; RL J. Virol. 59:284-291(1986). RN [3] RP NUCLEOTIDE SEQUENCE. RC STRAIN=NL4-3; RA Salminen M.S.; RL Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases. RN [4] RP PROTEIN SEQUENCE. RX MEDLINE=97271389; PubMed=9126340; DOI=10.1006/bbrc.1997.6349; RA Macreadie I.G., Lowe M.G., Curtain C.C., Hewish D., Azad A.A.; RT "Cytotoxicity resulting from addition of HIV-1 Nef N-terminal peptides RT to yeast and bacterial cells."; RL Biochem. Biophys. Res. Commun. 232:707-711(1997). RN [5] RP NUCLEOTIDE SEQUENCE. RX MEDLINE=92333269; PubMed=1629704; RA Smith J., Azad A., Deacon N.; RT "Identification of two novel human immunodeficiency virus type 1 RT splice acceptor sites in infected T cell lines."; RL J. Gen. Virol. 73:1825-1828(1992). RN [6] RP PROTEIN SEQUENCE. RX MEDLINE=94229079; PubMed=8174561; RA Freund J., Kellner R., Houthaeve T., Kalbitzer H.R.; RT "Stability and proteolytic domains of Nef protein from human RT immunodeficiency virus (HIV) type 1."; RL Eur. J. Biochem. 221:811-819(1994). RN [7] RP NUCLEOTIDE SEQUENCE. RX PubMed=16032733; DOI=10.1002/jmv.20408; RA Parreira R., Padua E., Piedade J., Venenno T., Paixao M.T., RA Esteves A.; RT "Genetic analysis of human immunodeficiency virus type 1 nef in RT Portugal: subtyping, identification of mosaic genes, and amino acid RT sequence variability."; RL J. Med. Virol. 77:8-16(2005). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U26942; AAB60579.1; -; Genomic_DNA. DR EMBL; AJ850868; CAH64567.1; -; Genomic_DNA. DR PIR; JC5400; JC5400. DR PIR; JQ1620; JQ1620. DR PIR; S03244; S03244. DR PIR; S43467; S43467. DR HSSP; P03406; 1AVZ. DR SMR; Q90VU7; 2-57, 56-205. DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB. DR GO; GO:0005856; C:cytoskeleton; ISS:UniProtKB. DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB. DR GO; GO:0051117; F:ATPase binding; ISS:UniProtKB. DR GO; GO:0005516; F:calmodulin binding; ISS:UniProtKB. DR GO; GO:0042609; F:CD4 receptor binding; ISS:UniProtKB. DR GO; GO:0042288; F:MHC class I protein binding; ISS:UniProtKB. DR GO; GO:0019901; F:protein kinase binding; ISS:UniProtKB. DR GO; GO:0017124; F:SH3 domain binding; ISS:UniProtKB. DR GO; GO:0031996; F:thioesterase binding; ISS:UniProtKB. DR GO; GO:0045225; P:negative regulation of CD4 biosynthetic pro...; ISS:UniProtKB. DR GO; GO:0001915; P:negative regulation of T cell mediated cyto...; ISS:UniProtKB. DR GO; GO:0009405; P:pathogenesis; ISS:UniProtKB. DR GO; GO:0050848; P:regulation of calcium-mediated signaling; ISS:UniProtKB. DR GO; GO:0050863; P:regulation of T cell activation; ISS:UniProtKB. DR GO; GO:0046776; P:suppression by virus of MHC class I cell su...; ISS:UniProtKB. DR GO; GO:0019058; P:viral infectious cycle; ISS:UniProtKB. DR InterPro; IPR001558; HIV_Nef. DR Gene3D; G3DSA:3.30.62.10; HIV_Nef; 1. DR Pfam; PF00469; F-protein; 1. DR ProDom; PD000031; HIV_Nef; 1. KW Lipoprotein; Myristate; Viral immunoevasion; Virulence. SQ SEQUENCE 206 AA; 23367 MW; 65AF3B6184DC2FE7 CRC64; MGGKWSKSSV IGWPAVRERM RRAEPAADGV GAVSRDLEKH GAITSSNTAA NNAACAWLEA QEEEEVGFPV TPQVPLRPMT YKAAVDLSHF LKEKGGLEGL IHSQRRQDIL DLWIYHTQGY FPDWQNYTPG PGVRYPLTFG WCYKLVPVEP DKVEEANKGE NTSLLHPVSL HGMDDPEREV LEWRFDSRLA FHHVARELHP EYFKNC //