ID Q90491_DANRE Unreviewed; 583 AA. AC Q90491; DT 01-NOV-1996, integrated into UniProtKB/TrEMBL. DT 01-NOV-1996, sequence version 1. DT 02-OCT-2024, entry version 158. DE RecName: Full=Transcription factor E2-alpha {ECO:0000256|ARBA:ARBA00041064}; DE AltName: Full=Transcription factor 3 {ECO:0000256|ARBA:ARBA00041234}; GN Name=tcf3a {ECO:0000313|RefSeq:NP_571169.1, GN ECO:0000313|ZFIN:ZDB-GENE-990415-51}; GN Synonyms=e12 {ECO:0000313|RefSeq:NP_571169.1}, tcf3 GN {ECO:0000313|RefSeq:NP_571169.1}, zfe12 GN {ECO:0000313|RefSeq:NP_571169.1}, zgc:113926 GN {ECO:0000313|RefSeq:NP_571169.1}; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955 {ECO:0000313|EMBL:CAA54305.1}; RN [1] {ECO:0000313|EMBL:CAA54305.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=WT {ECO:0000313|EMBL:CAA54305.1}; RC TISSUE=Whole embryo {ECO:0000313|EMBL:CAA54305.1}; RX PubMed=7918099; DOI=10.1016/0925-4773(94)90077-9; RA Wuelbeck C, Fromental-Ramain C, Campos-Ortega JAA; RT "The HLH domain of a zebrafish HE12 homologue can partially substitute for RT functions of the HLH domain of Drosophila DAUGHTERLESS."; RL Mech. Dev. 46:73-85(1994). RN [2] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=17517144; RA Chong S.W., Nguyet L.M., Jiang Y.J., Korzh V.; RT "The chemokine Sdf-1 and its receptor Cxcr4 are required for formation of RT muscle in zebrafish."; RL BMC Dev. Biol. 7:54-54(2007). RN [3] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=18062957; RA Bonner J., Gribble S.L., Veien E.S., Nikolaus O.B., Weidinger G., RA Dorsky R.I.; RT "Proliferation and patterning are mediated independently in the dorsal RT spinal cord downstream of canonical Wnt signaling."; RL Dev. Biol. 313:398-407(2008). RN [4] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=19303855; RA Goessling W., North T.E., Loewer S., Lord A.M., Lee S., Stoick-Cooper C.L., RA Weidinger G., Puder M., Daley G.Q., Moon R.T., Zon L.I.; RT "Genetic interaction of PGE2 and Wnt signaling regulates developmental RT specification of stem cells and regeneration."; RL Cell 136:1136-1147(2009). RN [5] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=19176587; RA Gribble S.L., Kim H.S., Bonner J., Wang X., Dorsky R.I.; RT "Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression."; RL Development 136:781-789(2009). RN [6] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=19449054; DOI=10.1007/s00239-009-9232-7; RA Wang Y., Chen K., Yao Q., Zheng X., Yang Z.; RT "Phylogenetic analysis of zebrafish basic helix-loop-helix transcription RT factors."; RL J. Mol. Evol. 68:629-640(2009). RN [7] {ECO:0000313|Proteomes:UP000000437} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=23594743; DOI=10.1038/nature12111; RG Genome Reference Consortium Zebrafish; RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J., RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D., RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B., RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J., RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D., RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B., RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P., RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., RA Rogers J., Stemple D.L.; RT "The zebrafish reference genome sequence and its relationship to the human RT genome."; RL Nature 496:498-503(2013). RN [8] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=27189481; RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I., RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y., RA Bobe J.; RT "Gene evolution and gene expression after whole genome duplication in fish: RT the PhyloFish database."; RL BMC Genomics 17:368-368(2016). RN [9] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=28654864; RA Yu C., Yao X., Zhao L., Wang P., Zhang Q., Zhao C., Yao S., Wei Y.; RT "Wolf-Hirschhorn Syndrome Candidate 1 (whsc1) Functions as a Tumor RT Suppressor by Governing Cell Differentiation."; RL Neoplasia 19:606-616(2017). RN [10] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=29911973; RA Ladam F., Stanney W., Donaldson I.J., Yildiz O., Bobola N., RA Sagerstrom C.G.; RT "TALE factors use two distinct functional modes to control an essential RT zebrafish gene expression program."; RL Elife 7:e36144-e36144(2018). RN [11] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=30045885; DOI=10.1098/rsob.180043; RA Chi Y., Huang Z., Chen Q., Xiong X., Chen K., Xu J., Zhang Y., Zhang W.; RT "Loss of runx1 function results in B cell immunodeficiency but not T cell RT in adult zebrafish."; RL Open Biol. 8:180043-180043(2018). RN [12] {ECO:0000313|RefSeq:NP_571169.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RX PubMed=30044923; RA Cai C., Sang C., Du J., Jia H., Tu J., Wan Q., Bao B., Xie S., Huang Y., RA Li A., Li J., Yang K., Wang S., Lu Q.; RT "Knockout of tnni1b in zebrafish causes defects in atrioventricular valve RT development via the inhibition of the myocardial wnt signaling pathway."; RL FASEB J. 33:696-710(2019). RN [13] {ECO:0000313|RefSeq:NP_571169.1} RP IDENTIFICATION. RC STRAIN=Wild-type {ECO:0000313|RefSeq:NP_571169.1}; RG RefSeq; RL Submitted (JUN-2024) to UniProtKB. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X76997; CAA54305.1; -; mRNA. DR PIR; I50518; I50518. DR RefSeq; NP_571169.1; NM_131094.1. DR GeneID; 30310; -. DR KEGG; dre:30310; -. DR AGR; ZFIN:ZDB-GENE-990415-51; -. DR CTD; 30310; -. DR ZFIN; ZDB-GENE-990415-51; tcf3a. DR OrthoDB; 4221520at2759; -. DR PhylomeDB; Q90491; -. DR Proteomes; UP000000437; Chromosome 2. DR GO; GO:0000785; C:chromatin; IBA:GO_Central. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro. DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:ZFIN. DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central. DR GO; GO:0055002; P:striated muscle cell development; IMP:ZFIN. DR CDD; cd18945; bHLH_E-protein_TCF4_E2-2; 1. DR Gene3D; 4.10.280.10; Helix-loop-helix DNA-binding domain; 1. DR InterPro; IPR011598; bHLH_dom. DR InterPro; IPR036638; HLH_DNA-bd_sf. DR InterPro; IPR051098; NeuroDiff_E-box_TFs. DR PANTHER; PTHR11793; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR; 1. DR PANTHER; PTHR11793:SF7; TRANSCRIPTION FACTOR E2-ALPHA; 1. DR Pfam; PF00010; HLH; 1. DR SMART; SM00353; HLH; 1. DR SUPFAM; SSF47459; HLH, helix-loop-helix DNA-binding domain; 1. DR PROSITE; PS50888; BHLH; 1. PE 1: Evidence at protein level; KW DNA-binding {ECO:0000256|ARBA:ARBA00023125}; KW Nucleus {ECO:0000256|ARBA:ARBA00023242}; KW Proteomics identification {ECO:0007829|PeptideAtlas:Q90491}; KW Reference proteome {ECO:0000313|Proteomes:UP000000437}; KW Transcription {ECO:0000256|ARBA:ARBA00023163}; KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015}. FT DOMAIN 488..541 FT /note="BHLH" FT /evidence="ECO:0000259|PROSITE:PS50888" FT REGION 25..56 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 105..166 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 281..321 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 400..489 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 29..56 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 125..139 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 148..162 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 281..311 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 400..429 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 430..466 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 583 AA; 62626 MW; 2BDC4EDB81E74F44 CRC64; MATVGTDMEL NDLLDFSIMF PHPMSNGKSR GPNIPSSQFG LDDRNDSGSW GSEANSPSFG ARLYGDGHHY PEHDNMYNLE IDGKSERGYP VTQLLPGEIN IAASDALSPP GMKGSSQFYS PHRRRPAESS IDPLPKKRKV PHGLPTSISA YPGSTGDDYN QDSAGYPGSK PGNLYPGFFN VQEGLPDHWG QSGYPPVMSN FINPQKPQPL PVSPQNYPLH GSEVNLPSSF HSVPAGYGVP NHTPPISSSE GIMGNRGANA GSSGAEIGKA LASIYPSDNN GSNFSSTPST PVGSPSNVAA SASQWPKGSS QPAPSPGFEA GLAQSKLDDR LGEALHVLRN HHAVGTDMQS LLSAAISGHS AGAAALGSLR QAFGLANRLP GLMSNHSEDA AGLPQSSALL QGHHTSHQPP PSSQSDTFTS LSLARSSHAS SSDIKREDKD DDENLSIADK SEDEKKDGKS RTRTSQDEDE DEDDEDLPVE VKAERERERR MANNARERLR VRDINEAFKE LGRMCQLHLS NEKPQTKLLI LHQAVNVILN LEQQVRERNL NPKAACLKRR EEEKVTGEAQ MLSGLGGDGH AHM //