ID FADB_SALTI Reviewed; 729 AA. AC Q8Z3C6; DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2002, sequence version 1. DT 23-JAN-2007, entry version 35. DE Fatty oxidation complex subunit alpha [Includes: Enoyl-CoA hydratase DE (EC 4.2.1.17); Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase DE (EC 5.3.3.8); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); 3- DE hydroxybutyryl-CoA epimerase (EC 5.1.2.3)]. GN Name=fadB; OrderedLocusNames=STY3577, t3315; OS Salmonella typhi. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Salmonella. OX NCBI_TaxID=601; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CT18; RX MEDLINE=21534947; PubMed=11677608; DOI=10.1038/35101607; RA Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., RA Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., RA Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., RA Cronin A., Davis P., Davies R.M., Dowd L., White N., Farrar J., RA Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., RA Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., RA Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., RA Whitehead S., Barrell B.G.; RT "Complete genome sequence of a multiple drug resistant Salmonella RT enterica serovar Typhi CT18."; RL Nature 413:848-852(2001). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700931 / Ty2; RX MEDLINE=22531367; PubMed=12644504; RX DOI=10.1128/JB.185.7.2330-2337.2003; RA Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., RA Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.; RT "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 RT and CT18."; RL J. Bacteriol. 185:2330-2337(2003). CC -!- FUNCTION: Catalyzes the formation of an hydroxyacyl-CoA by CC addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA CC epimerase and 3-hydroxyacyl-CoA dehydrogenase activities. Involved CC in the aerobic and anaerobic degradation of long-chain fatty acids CC (By similarity). CC -!- CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA CC + NADH. CC -!- CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CC CoA + H(2)O. CC -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3- CC hydroxybutanoyl-CoA. CC -!- CATALYTIC ACTIVITY: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl- CC CoA. CC -!- PATHWAY: Lipid catabolism; beta-oxidation cycle; from acyl-CoA: CC step B. CC -!- SUBUNIT: Heterotetramer of two alpha chains (fadB) and two beta CC chains (fadA) (By similarity). CC -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA CC hydratase/isomerase family. CC -!- SIMILARITY: In the C-terminal section; belongs to the 3- CC hydroxyacyl-CoA dehydrogenase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL627278; CAD07910.1; -; Genomic_DNA. DR EMBL; AE014613; AAO70843.1; -; Genomic_DNA. DR HSSP; Q16836; 1F0Y. DR GenomeReviews; AL513382_GR; STY3577. DR GenomeReviews; AE014613_GR; t3315. DR KEGG; stt:t3315; -. DR KEGG; sty:STY3577; -. DR BioCyc; SENT209261:T3315-MONOMER; -. DR BioCyc; SENT90370:STY3577-MONOMER; -. DR GO; GO:0016042; P:lipid catabolism; IEA:HAMAP. DR HAMAP; MF_01621; -; 1. DR InterPro; IPR006108; 3HC_DH_C. DR InterPro; IPR006180; 3HCDH. DR InterPro; IPR006176; 3HCDH_NAD_bd. DR InterPro; IPR008927; 6DGDH_C_like. DR InterPro; IPR001753; EnCoA_hydratase. DR InterPro; IPR012799; FadB. DR Gene3D; G3DSA:1.10.770.10; 3HCDH_C; 2. DR Pfam; PF00725; 3HCDH; 2. DR Pfam; PF02737; 3HCDH_N; 1. DR Pfam; PF00378; ECH; 1. DR TIGRFAMs; TIGR02437; FadB; 1. DR PROSITE; PS00067; 3HCDH; 1. DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1. KW Complete proteome; Fatty acid metabolism; Isomerase; KW Lipid degradation; Lipid metabolism; Lyase; Multifunctional enzyme; KW NAD; Oxidoreductase. FT CHAIN 1 729 Fatty oxidation complex subunit alpha. FT /FTId=PRO_0000109286. FT REGION 1 189 Enoyl-CoA hydratase/isomerase (By FT similarity). FT REGION 311 729 3-hydroxyacyl-CoA dehydrogenase (By FT similarity). SQ SEQUENCE 729 AA; 79642 MW; C34C68ECFF35713C CRC64; MLYKGDTLYL DWLEDGIAEL VFDAPGSVNK LDTATVASLG QALEVLEKQH DLKGLLLRSN KAAFIVGADI TEFLSLFLVP EEQLSQWLHF ANSVFNRLED LPVPTLAAVN GYALGGGCEC VLATDYRLAT PDLRIGLPET KLGIMPGFGG SVRLPRMLGA DSALEIIAAG KDVGAEHALK IGLVDGVVKQ EKLIEGAIAV LRQAITGDLD WRAKRQPKLE PLKLSKIEAA MSFTIAKGMV AQTAGKHYPA PMTAVKTIEA AARFGREEAL NLENKSFVPL AHTNEARALV GIFLNDQYVK GKAKKLTKDI ETPKQAAVLG AGIMGGGIAY QSAWKGVPVI MKDINDKSLN LGMTEAAKLL NKQLERGKID GLKLAGVIST IHPTLDYAGF DRVDVVVEAV VENPKVKKAV LAETEQKVRP ETVLASNTST IPIGELASAL ERPENFCGMH FFNPVHRMPL VEIIRGEKSS DETIAKVVAW ASKMGKTPIV VNNCPGFFVN RVLFPYFAGF SQLLRDGADF RKVDKVMEKQ FGWPMGPAYL LDVVGIDTAH HAQAVMAAGF PQRMQKEYRD AIDALFDASR FGQKNGLGFW RYKEDSKGKP KKEEDAAVDD LLASVSQTKR DFSDDEIIAR MMIPMINEVV RCLEEGIIAS PAEADMALVY GLGFPPFHGG AFRWLDTQGS AKYLDMAQQY QHLGPLYEVP EGLRDKTRHN EPYYPPVEPA RPVGSLKTA //