ID GAB3_HUMAN Reviewed; 586 AA. AC Q8WWW8; A6NHF8; E9PB44; DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2002, sequence version 1. DT 07-SEP-2016, entry version 105. DE RecName: Full=GRB2-associated-binding protein 3; DE AltName: Full=GRB2-associated binder 3; DE AltName: Full=Growth factor receptor bound protein 2-associated protein 3; GN Name=GAB3; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RC TISSUE=Dendritic cell, and Eosinophil; RX PubMed=11739737; DOI=10.1128/MCB.22.1.231-244.2002; RA Wolf I., Jenkins B.J., Liu Y., Seiffert M., Custodio J.M., Young P., RA Rohrschneider L.R.; RT "Gab3, a new DOS/Gab family member, facilitates macrophage RT differentiation."; RL Mol. Cell. Biol. 22:231-244(2002). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). RC TISSUE=Thalamus, and Trachea; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15772651; DOI=10.1038/nature03440; RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., RA Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., RA Jones M.C., Hurles M.E., Andrews T.D., Scott C.E., Searle S., RA Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., RA Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., RA Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., RA Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., RA Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., RA Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., RA Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., RA Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., RA Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., RA Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., RA Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., RA Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., RA Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., RA Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., RA Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., RA Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., RA Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., RA Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., RA Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., RA Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., RA Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., RA de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., RA Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., RA Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., RA Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., RA Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., RA Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., RA Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., RA Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., RA Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., RA Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., RA Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., RA Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., RA Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., RA Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., RA Williams G., Williams L., Williamson A., Williamson H., Wilming L., RA Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., RA Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., RA Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., RA Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., RA Gibbs R.A., Beck S., Rogers J., Bentley D.R.; RT "The DNA sequence of the human X chromosome."; RL Nature 434:325-337(2005). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., RA Venter J.C.; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. RN [5] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-344 AND SER-482, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Erythroleukemia; RX PubMed=23186163; DOI=10.1021/pr300630k; RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., RA Mohammed S.; RT "Toward a comprehensive characterization of a human cancer cell RT phosphoproteome."; RL J. Proteome Res. 12:260-271(2013). CC -!- SUBUNIT: Interacts with PIK3R/p85, SHP2 and GRAP2/MONA (By CC similarity). May interact with Grb2. {ECO:0000250}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q8WWW8-1; Sequence=Displayed; CC Name=2; CC IsoId=Q8WWW8-2; Sequence=VSP_045033; CC Note=No experimental confirmation available.; CC Name=3; CC IsoId=Q8WWW8-3; Sequence=VSP_045033, VSP_055259; CC Note=No experimental confirmation available.; CC -!- PTM: Phosphorylated on tyrosine residue(s) after macrophage CC colony-stimulating factor (M-CSF) receptor stimulation. CC {ECO:0000250}. CC -!- SIMILARITY: Belongs to the GAB family. {ECO:0000305}. CC -!- SIMILARITY: Contains 1 PH domain. {ECO:0000255|PROSITE- CC ProRule:PRU00145}. CC -!- SEQUENCE CAUTION: CC Sequence=AK307927; Type=Erroneous termination; Positions=587; Note=Translated as stop.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY057989; AAL25825.1; -; mRNA. DR EMBL; AK126283; BAG54302.1; -; mRNA. DR EMBL; AK307927; -; NOT_ANNOTATED_CDS; mRNA. DR EMBL; AC107450; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AC109993; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471172; EAW72665.1; -; Genomic_DNA. DR EMBL; CH471172; EAW72667.1; -; Genomic_DNA. DR CCDS; CCDS14760.1; -. [Q8WWW8-1] DR CCDS; CCDS48198.1; -. [Q8WWW8-2] DR CCDS; CCDS65357.1; -. [Q8WWW8-3] DR RefSeq; NP_001075042.1; NM_001081573.2. [Q8WWW8-2] DR RefSeq; NP_001269212.1; NM_001282283.1. [Q8WWW8-3] DR RefSeq; NP_542179.1; NM_080612.3. [Q8WWW8-1] DR RefSeq; XP_005274705.1; XM_005274648.1. [Q8WWW8-2] DR RefSeq; XP_006724867.1; XM_006724804.1. [Q8WWW8-2] DR UniGene; Hs.496982; -. DR ProteinModelPortal; Q8WWW8; -. DR SMR; Q8WWW8; 26-109. DR BioGrid; 126581; 7. DR IntAct; Q8WWW8; 1. DR MINT; MINT-1594309; -. DR STRING; 9606.ENSP00000399588; -. DR iPTMnet; Q8WWW8; -. DR PhosphoSite; Q8WWW8; -. DR BioMuta; GAB3; -. DR DMDM; 74716251; -. DR MaxQB; Q8WWW8; -. DR PaxDb; Q8WWW8; -. DR PeptideAtlas; Q8WWW8; -. DR PRIDE; Q8WWW8; -. DR DNASU; 139716; -. DR Ensembl; ENST00000369568; ENSP00000358581; ENSG00000160219. [Q8WWW8-3] DR Ensembl; ENST00000369575; ENSP00000358588; ENSG00000160219. [Q8WWW8-1] DR Ensembl; ENST00000424127; ENSP00000399588; ENSG00000160219. [Q8WWW8-2] DR GeneID; 139716; -. DR KEGG; hsa:139716; -. DR UCSC; uc004fmj.3; human. [Q8WWW8-1] DR CTD; 139716; -. DR GeneCards; GAB3; -. DR HGNC; HGNC:17515; GAB3. DR HPA; HPA010090; -. DR MIM; 300482; gene. DR neXtProt; NX_Q8WWW8; -. DR PharmGKB; PA28479; -. DR eggNOG; ENOG410IEG0; Eukaryota. DR eggNOG; ENOG4111RDE; LUCA. DR GeneTree; ENSGT00510000046662; -. DR HOGENOM; HOG000236270; -. DR HOVERGEN; HBG107126; -. DR InParanoid; Q8WWW8; -. DR OMA; EFQNNFV; -. DR OrthoDB; EOG091G03BY; -. DR PhylomeDB; Q8WWW8; -. DR TreeFam; TF329487; -. DR ChiTaRS; GAB3; human. DR GenomeRNAi; 139716; -. DR PRO; PR:Q8WWW8; -. DR Proteomes; UP000005640; Chromosome X. DR Bgee; ENSG00000160219; -. DR CleanEx; HS_GAB3; -. DR ExpressionAtlas; Q8WWW8; baseline and differential. DR Genevisible; Q8WWW8; HS. DR GO; GO:0030225; P:macrophage differentiation; IEA:Ensembl. DR Gene3D; 2.30.29.30; -; 1. DR InterPro; IPR011993; PH_dom-like. DR InterPro; IPR001849; PH_domain. DR Pfam; PF00169; PH; 1. DR SMART; SM00233; PH; 1. DR SUPFAM; SSF50729; SSF50729; 1. DR PROSITE; PS50003; PH_DOMAIN; 1. PE 1: Evidence at protein level; KW Alternative splicing; Complete proteome; Phosphoprotein; Polymorphism; KW Reference proteome. FT CHAIN 1 586 GRB2-associated-binding protein 3. FT /FTId=PRO_0000318939. FT DOMAIN 5 117 PH. {ECO:0000255|PROSITE- FT ProRule:PRU00145}. FT MOD_RES 344 344 Phosphoserine. FT {ECO:0000244|PubMed:23186163}. FT MOD_RES 482 482 Phosphoserine. FT {ECO:0000244|PubMed:23186163}. FT VAR_SEQ 125 125 A -> AA (in isoform 2 and isoform 3). FT {ECO:0000303|PubMed:14702039}. FT /FTId=VSP_045033. FT VAR_SEQ 510 548 Missing (in isoform 3). FT {ECO:0000303|PubMed:14702039}. FT /FTId=VSP_055259. FT VARIANT 237 237 P -> S (in dbSNP:rs17281349). FT /FTId=VAR_038917. SQ SEQUENCE 586 AA; 65589 MW; 9DE0223C34AD8FFE CRC64; MSAGDAVCTG WLVKSPPERK LQRYAWRKRW FVLRRGRMSG NPDVLEYYRN KHSSKPIRVI DLSECAVWKH VGPSFVRKEF QNNFVFIVKT TSRTFYLVAK TEQEMQVWVH SISQVCNLGH LEDGADSMES LSYTPSSLQP SSASSLLTAH AASSSLPRDD PNTNAVATEE TRSESELLFL PDYLVLSNCE TGRLHHTSLP TRCDSWSNSD RSLEQASFDD VFVDCLQPLP SSHLVHPSCH GSGAQEVPSS RPQAALIWSR EINGPPRDHL SSSPLLESSL SSTIQVDKNQ GSLPCGAKEL DIMSNTPPPR PPKPSHLSER RQEEWSTHSG SKKPECTLVP RRISLSGLDN MRTWKADVEG QSLRHRDKRL SLNLPCRFSP MYPTASASIE DSYVPMSPQA GASGLGPHCS PDDYIPMNSG SISSPLPELP ANLEPPPVNR DLKPQRKSRP PPLDLRNLSI IREHASLTRT RTVPCSRTSF LSPERNGINS ARFFANPVSR EDEESYIEME EHRTASSLSS GALTWTKKFS LDYLALDFNS ASPAPMQQKL LLSEEQRVDY VQVDEQKTQA LQSTKQEWTD ERQSKV //