ID CPLX3_HUMAN Reviewed; 158 AA. AC Q8WVH0; D3DW66; Q8TEM6; Q9H818; DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2002, sequence version 1. DT 28-JUN-2023, entry version 136. DE RecName: Full=Complexin-3; DE AltName: Full=Complexin III; DE Short=CPX III; DE Flags: Precursor; GN Name=CPLX3; ORFNames=Nbla11589; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Brain; RX PubMed=15911881; DOI=10.1083/jcb.200502115; RA Reim K., Wegmeyer H., Brandstaetter J.H., Xue M., Rosenmund C., RA Dresbach T., Hofmann K., Brose N.; RT "Structurally and functionally unique complexins at retinal ribbon RT synapses."; RL J. Cell Biol. 169:669-680(2005). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Spleen; RA Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.; RT "The nucleotide sequence of a long cDNA clone isolated from human spleen."; RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Neuroblastoma; RX PubMed=12880961; DOI=10.1016/s0304-3835(03)00085-5; RA Ohira M., Morohashi A., Nakamura Y., Isogai E., Furuya K., Hamano S., RA Machida T., Aoyama M., Fukumura M., Miyazaki K., Suzuki Y., Sugano S., RA Hirato J., Nakagawara A.; RT "Neuroblastoma oligo-capping cDNA project: toward the understanding of the RT genesis and biology of neuroblastoma."; RL Cancer Lett. 197:63-68(2003). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., RA Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., RA Hunkapiller M.W., Myers E.W., Venter J.C.; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Brain; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). CC -!- FUNCTION: Complexin that regulates SNARE protein complex-mediated CC synaptic vesicle fusion (By similarity). Required for the maintenance CC of synaptic ultrastructure in the adult retina (By similarity). CC Positively regulates synaptic transmission through synaptic vesicle CC availability and exocytosis of neurotransmitters at photoreceptor CC ribbon synapses in the retina (By similarity). Suppresses tonic CC photoreceptor activity and baseline 'noise' by suppression of Ca(2+) CC vesicle tonic release and the facilitation of evoked synchronous and CC asynchronous Ca(2+) vesicle release (By similarity). CC {ECO:0000250|UniProtKB:Q8R1B5}. CC -!- SUBUNIT: Binds to the SNARE core complex containing SNAP25, VAMP2 and CC STX1A. {ECO:0000250|UniProtKB:Q8R1B5}. CC -!- SUBCELLULAR LOCATION: Synapse {ECO:0000250|UniProtKB:Q8R1B5}. Cell CC membrane; Lipid-anchor {ECO:0000250|UniProtKB:Q8R1B5}. Note=Enriched at CC the synaptic terminal (By similarity). Localized at glycinergic CC synaptic contacts of AII amacrine cells with OFF cone bipolar cells in CC the OFF sublamina of the retina inner nuclear layer (By similarity). CC {ECO:0000250|UniProtKB:Q8R1B5}. CC -!- PTM: Farnesylation mediates presynaptic targeting. CC {ECO:0000250|UniProtKB:Q8R1B5}. CC -!- SIMILARITY: Belongs to the complexin/synaphin family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=BAB84922.1; Type=Erroneous initiation; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY286501; AAP41127.1; -; mRNA. DR EMBL; AK074096; BAB84922.1; ALT_INIT; mRNA. DR EMBL; AB072900; BAE45711.1; -; mRNA. DR EMBL; AK024055; BAB14805.1; -; mRNA. DR EMBL; CH471136; EAW99306.1; -; Genomic_DNA. DR EMBL; CH471136; EAW99307.1; -; Genomic_DNA. DR EMBL; BC018026; AAH18026.1; -; mRNA. DR CCDS; CCDS32294.1; -. DR RefSeq; NP_001025176.1; NM_001030005.2. DR AlphaFoldDB; Q8WVH0; -. DR SMR; Q8WVH0; -. DR BioGRID; 534761; 26. DR CORUM; Q8WVH0; -. DR IntAct; Q8WVH0; 17. DR STRING; 9606.ENSP00000378464; -. DR GlyGen; Q8WVH0; 1 site, 1 O-linked glycan (1 site). DR PhosphoSitePlus; Q8WVH0; -. DR BioMuta; CPLX3; -. DR DMDM; 74751549; -. DR MassIVE; Q8WVH0; -. DR PaxDb; Q8WVH0; -. DR PeptideAtlas; Q8WVH0; -. DR ProteomicsDB; 74793; -. DR Antibodypedia; 43203; 158 antibodies from 22 providers. DR DNASU; 594855; -. DR Ensembl; ENST00000395018.6; ENSP00000378464.4; ENSG00000213578.6. DR GeneID; 594855; -. DR KEGG; hsa:594855; -. DR MANE-Select; ENST00000395018.6; ENSP00000378464.4; NM_001030005.3; NP_001025176.1. DR UCSC; uc059llo.1; human. DR AGR; HGNC:27652; -. DR CTD; 594855; -. DR GeneCards; CPLX3; -. DR HGNC; HGNC:27652; CPLX3. DR HPA; ENSG00000213578; Group enriched (heart muscle, lymphoid tissue, prostate, retina). DR MIM; 609585; gene. DR neXtProt; NX_Q8WVH0; -. DR OpenTargets; ENSG00000213578; -. DR PharmGKB; PA142672081; -. DR VEuPathDB; HostDB:ENSG00000213578; -. DR eggNOG; ENOG502QZ0D; Eukaryota. DR GeneTree; ENSGT00950000182938; -. DR HOGENOM; CLU_141096_0_0_1; -. DR InParanoid; Q8WVH0; -. DR OMA; ERDAQFM; -. DR OrthoDB; 5354212at2759; -. DR PhylomeDB; Q8WVH0; -. DR TreeFam; TF331867; -. DR PathwayCommons; Q8WVH0; -. DR SignaLink; Q8WVH0; -. DR BioGRID-ORCS; 594855; 13 hits in 1142 CRISPR screens. DR GenomeRNAi; 594855; -. DR Pharos; Q8WVH0; Tbio. DR PRO; PR:Q8WVH0; -. DR Proteomes; UP000005640; Chromosome 15. DR RNAct; Q8WVH0; protein. DR Bgee; ENSG00000213578; Expressed in spleen and 66 other tissues. DR ExpressionAtlas; Q8WVH0; baseline. DR Genevisible; Q8WVH0; HS. DR GO; GO:0005829; C:cytosol; IEA:Ensembl. DR GO; GO:0098684; C:photoreceptor ribbon synapse; IEA:Ensembl. DR GO; GO:0048787; C:presynaptic active zone membrane; IEA:Ensembl. DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central. DR GO; GO:0030672; C:synaptic vesicle membrane; IEA:Ensembl. DR GO; GO:0043195; C:terminal bouton; IBA:GO_Central. DR GO; GO:0005326; F:neurotransmitter transmembrane transporter activity; IEA:Ensembl. DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central. DR GO; GO:0019905; F:syntaxin binding; IEA:InterPro. DR GO; GO:0030073; P:insulin secretion; IEA:Ensembl. DR GO; GO:0046928; P:regulation of neurotransmitter secretion; IBA:GO_Central. DR GO; GO:0031630; P:regulation of synaptic vesicle fusion to presynaptic active zone membrane; IEA:Ensembl. DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW. DR GO; GO:0016079; P:synaptic vesicle exocytosis; IBA:GO_Central. DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW. DR CDD; cd22809; Complexin_NTD_CPLX_III_IV; 1. DR Gene3D; 1.20.5.580; Single Helix bin; 1. DR InterPro; IPR008849; Synaphin. DR PANTHER; PTHR16705; COMPLEXIN; 1. DR PANTHER; PTHR16705:SF5; COMPLEXIN-3; 1. DR Pfam; PF05835; Synaphin; 1. PE 2: Evidence at transcript level; KW Cell membrane; Coiled coil; Exocytosis; Lipoprotein; Membrane; Methylation; KW Neurotransmitter transport; Prenylation; Reference proteome; KW Sensory transduction; Synapse; Transport; Vision. FT CHAIN 1..155 FT /note="Complexin-3" FT /id="PRO_0000239272" FT PROPEP 156..158 FT /note="Removed in mature form" FT /evidence="ECO:0000250" FT /id="PRO_0000240233" FT REGION 13..47 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 74..99 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COILED 39..74 FT /evidence="ECO:0000255" FT COMPBIAS 24..47 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 155 FT /note="Cysteine methyl ester" FT /evidence="ECO:0000250|UniProtKB:Q8R1B5" FT LIPID 155 FT /note="S-farnesyl cysteine" FT /evidence="ECO:0000250|UniProtKB:Q8R1B5" FT CONFLICT 19 FT /note="S -> G (in Ref. 4; BAB14805)" FT /evidence="ECO:0000305" SQ SEQUENCE 158 AA; 17557 MW; 057B4DE06DB6EA0F CRC64; MAFMVKTMVG GQLKNLTGSL GGGEDKGDGD KSAAEAQGMS REEYEEYQKQ LVEEKMERDA QFTQRKAERA TLRSHFRDKY RLPKNETDES QIQMAGGDVE LPRELAKMIE EDTEEEEEKA SVLGQLASLP GLNLGSLKDK AQATLGDLKQ SAEKCHVM //