ID Q8WSF8_APLCA Unreviewed; 236 AA. AC Q8WSF8; DT 01-MAR-2002, integrated into UniProtKB/TrEMBL. DT 01-MAR-2002, sequence version 1. DT 03-SEP-2014, entry version 67. DE SubName: Full=Soluble acetylcholine receptor; OS Aplysia californica (California sea hare). OC Eukaryota; Metazoa; Lophotrochozoa; Mollusca; Gastropoda; OC Heterobranchia; Euthyneura; Euopisthobranchia; Aplysiomorpha; OC Aplysioidea; Aplysiidae; Aplysia. OX NCBI_TaxID=6500; RN [1] RP X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 18-236, AND GLYCOSYLATION AT RP ASN-91. RX PubMed=16193063; DOI=10.1038/sj.emboj.7600828; RA Hansen S.B., Sulzenbacher G., Huxford T., Marchot P., Taylor P., RA Bourne Y.; RT "Structures of Aplysia AChBP complexes with nicotinic agonists and RT antagonists reveal distinctive binding interfaces and conformations."; RL EMBO J. 24:3635-3646(2005). RN [2] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236. RX PubMed=15951818; DOI=10.1038/nsmb951; RA Celie P.H., Kasheverov I.E., Mordvintsev D.Y., Hogg R.C., RA van Nierop P., van Elk R., van Rossum-Fikkert S.E., Zhmak M.N., RA Bertrand D., Tsetlin V., Sixma T.K., Smit A.B.; RT "Crystal structure of nicotinic acetylcholine receptor homolog AChBP RT in complex with an alpha-conotoxin PnIA variant."; RL Nat. Struct. Mol. Biol. 12:582-588(2005). RN [3] RP NUCLEOTIDE SEQUENCE. RX PubMed=17190607; DOI=10.1016/j.cell.2006.09.052; RA Moroz L.L., Edwards J.R., Puthanveettil S.V., Kohn A.B., Ha T., RA Heyland A., Knudsen B., Sahni A., Yu F., Liu L., Jezzini S., RA Lovell P., Iannucculli W., Chen M., Nguyen T., Sheng H., Shaw R., RA Kalachikov S., Panchin Y.V., Farmerie W., Russo J.J., Ju J., RA Kandel E.R.; RT "Neuronal transcriptome of Aplysia: neuronal compartments and RT circuitry."; RL Cell 127:1453-1467(2006). RN [4] RP X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 20-236. RX PubMed=16505382; DOI=10.1073/pnas.0507889103; RA Ulens C., Hogg R.C., Celie P.H., Bertrand D., Tsetlin V., Smit A.B., RA Sixma T.K.; RT "Structural determinants of selective alpha-conotoxin binding to a RT nicotinic acetylcholine receptor homolog AChBP."; RL Proc. Natl. Acad. Sci. U.S.A. 103:3615-3620(2006). RN [5] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236. RX PubMed=17660751; DOI=10.1038/sj.emboj.7601785; RA Dutertre S., Ulens C., Buttner R., Fish A., van Elk R., Kendel Y., RA Hopping G., Alewood P.F., Schroeder C., Nicke A., Smit A.B., RA Sixma T.K., Lewis R.J.; RT "AChBP-targeted alpha-conotoxin correlates distinct binding RT orientations with nAChR subtype selectivity."; RL EMBO J. 26:3858-3867(2007). RN [6] RP X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 18-236 IN COMPLEX WITH RP COCAINE, AND GLYCOSYLATION AT ASN-91. RX PubMed=17481657; DOI=10.1016/j.jmb.2007.03.067; RA Hansen S.B., Taylor P.; RT "Galanthamine and non-competitive inhibitor binding to ACh-binding RT protein: evidence for a binding site on non-alpha-subunit interfaces RT of heteromeric neuronal nicotinic receptors."; RL J. Mol. Biol. 369:895-901(2007). RN [7] RP X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 18-225. RX PubMed=18940802; DOI=10.1074/jbc.C800194200; RA Hansen S.B., Wang H.L., Taylor P., Sine S.M.; RT "An ion selectivity filter in the extracellular domain of Cys-loop RT receptors reveals determinants for ion conductance."; RL J. Biol. Chem. 283:36066-36070(2008). RN [8] RP X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 18-236. RX PubMed=18477694; DOI=10.1073/pnas.0802197105; RA Talley T.T., Harel M., Hibbs R.E., Radic Z., Tomizawa M., Casida J.E., RA Taylor P.; RT "Atomic interactions of neonicotinoid agonists with AChBP: molecular RT recognition of the distinctive electronegative pharmacophore."; RL Proc. Natl. Acad. Sci. U.S.A. 105:7606-7611(2008). RN [9] RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 18-236, AND GLYCOSYLATION AT RP ASN-91. RX PubMed=19696737; DOI=10.1038/emboj.2009.227; RA Hibbs R.E., Sulzenbacher G., Shi J., Talley T.T., Conrod S., Kem W.R., RA Taylor P., Marchot P., Bourne Y.; RT "Structural determinants for interaction of partial agonists with RT acetylcholine binding protein and neuronal alpha7 nicotinic RT acetylcholine receptor."; RL EMBO J. 28:3040-3051(2009). RN [10] RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 20-236. RX PubMed=19331415; DOI=10.1021/jm801400g; RA Ulens C., Akdemir A., Jongejan A., van Elk R., Bertrand S., RA Perrakis A., Leurs R., Smit A.B., Sixma T.K., Bertrand D., RA de Esch I.J.; RT "Use of acetylcholine binding protein in the search for novel alpha7 RT nicotinic receptor ligands. In silico docking, pharmacological RT screening, and X-ray analysis."; RL J. Med. Chem. 52:2372-2383(2009). RN [11] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 18-236. RX PubMed=20224036; DOI=10.1073/pnas.0912372107; RA Bourne Y., Radic Z., Araoz R., Talley T.T., Benoit E., Servent D., RA Taylor P., Molgo J., Marchot P.; RT "Structural determinants in phycotoxins and AChBP conferring high RT affinity binding and nicotinic AChR antagonism."; RL Proc. Natl. Acad. Sci. U.S.A. 107:6076-6081(2010). RN [12] RP X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 18-236. RA Talley T.T., Harel M., Yamauchi G.J., Radic Z., Hansen S., Huxford T., RA Taylor P.W.; RT "The curare alkaloids: analyzing the poses of complexes with the RT acetylcholine binding protein in relation to structure and binding RT energetics."; RL Submitted (OCT-2010) to the PDB data bank. RN [13] RP X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS) OF 18-236 IN COMPLEX WITH RP MAGNESIUM. RA Talley T.T., Harel M., Yamauchi J.G., Radic Z., Hansen S., Huxford T., RA Taylor P.W.; RT "The Curare Alkaloids: Analyzing the Poses of Complexes with the RT Acetylcholine Binding Protein in Relation to Structure and Binding RT Energies."; RL Submitted (NOV-2010) to the PDB data bank. RN [14] RP X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS). RX PubMed=21920761; DOI=10.1016/j.bmc.2011.08.028; RA Akdemir A., Rucktooa P., Jongejan A., Elk R.v., Bertrand S., RA Sixma T.K., Bertrand D., Smit A.B., Leurs R., de Graaf C., RA de Esch I.J.; RT "Acetylcholine binding protein (AChBP) as template for hierarchical in RT silico screening procedures to identify structurally novel ligands for RT the nicotinic receptors."; RL Bioorg. Med. Chem. 19:6107-6119(2011). RN [15] RP X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 20-236. RX PubMed=21322593; DOI=10.1021/ja110571r; RA Edink E., Rucktooa P., Retra K., Akdemir A., Nahar T., Zuiderveld O., RA van Elk R., Janssen E., van Nierop P., van Muijlwijk-Koezen J., RA Smit A.B., Sixma T.K., Leurs R., de Esch I.J.; RT "Fragment growing induces conformational changes in acetylcholine- RT binding protein: a structural and thermodynamic analysis."; RL J. Am. Chem. Soc. 133:5363-5371(2011). RN [16] RP X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 20-236 IN COMPLEX WITH RP PHOSPHATE. RX PubMed=21115477; DOI=10.1074/jbc.M110.188730; RA Brams M., Gay E.A., Saez J.C., Guskov A., van Elk R., RA van der Schors R.C., Peigneur S., Tytgat J., Strelkov S.V., Smit A.B., RA Yakel J.L., Ulens C.; RT "Crystal structures of a cysteine-modified mutant in loop D of RT acetylcholine-binding protein."; RL J. Biol. Chem. 286:4420-4428(2011). RN [17] RP X-RAY CRYSTALLOGRAPHY (2.32 ANGSTROMS) OF 18-236, AND GLYCOSYLATION AT RP ASN-91. RX PubMed=22009746; DOI=10.1074/jbc.M111.286583; RA Nemecz A., Taylor P.; RT "Creating an ?7 nicotinic acetylcholine recognition domain from the RT acetylcholine-binding protein: crystallographic and ligand selectivity RT analyses."; RL J. Biol. Chem. 286:42555-42565(2011). RN [18] RP X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS) OF 20-236. RX PubMed=21468359; DOI=10.1371/journal.pbio.1001034; RA Brams M., Pandya A., Kuzmin D., van Elk R., Krijnen L., Yakel J.L., RA Tsetlin V., Smit A.B., Ulens C.; RT "A structural and mutagenic blueprint for molecular recognition of RT strychnine and d-tubocurarine by different cys-loop receptors."; RL PLoS Biol. 9:e1001034-e1001034(2011). RN [19] RP X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 18-236, AND GLYCOSYLATION AT RP ASN-91. RX PubMed=22394239; DOI=10.1021/ja3001858; RA Grimster N.P., Stump B., Fotsing J.R., Weide T., Talley T.T., RA Yamauchi J.G., Nemecz A., Kim C., Ho K.Y., Sharpless K.B., Taylor P., RA Fokin V.V.; RT "Generation of candidate ligands for nicotinic acetylcholine receptors RT via in situ click chemistry with a soluble acetylcholine binding RT protein template."; RL J. Am. Chem. Soc. 134:6732-6740(2012). RN [20] RP X-RAY CRYSTALLOGRAPHY (2.88 ANGSTROMS) OF 20-236. RX PubMed=22553201; DOI=10.1074/jbc.M112.360347; RA Rucktooa P., Haseler C.A., van Elk R., Smit A.B., Gallagher T., RA Sixma T.K.; RT "Structural characterization of binding mode of smoking cessation RT drugs to nicotinic acetylcholine receptors through study of ligand RT complexes with acetylcholine-binding protein."; RL J. Biol. Chem. 287:23283-23293(2012). RN [21] RP X-RAY CRYSTALLOGRAPHY (2.49 ANGSTROMS) OF 18-236. RA Filchakova O.M., Talley T.T., Reger A.S., Kim C., Ho K., Han K., RA Taylor P., McIntosh J.M.; RT "Pairwise interaction of alpha-conotoxin BuIA Pro6 with the beta RT subunit of nicotinic acetylcholine receptor."; RL Submitted (MAY-2012) to the PDB data bank. RN [22] RP X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 18-225. RA Mukhopadhyay S., Mesecar A.D.; RT "X-ray structure of Acetylcholine binding protein from Aplysia RT californica in presence of 3-((S)-azetidin-2-ylmethoxy)-5-((1S,2R)-2- RT (2-methoxyethyl)cyclopropyl)pyridine."; RL Submitted (JUN-2012) to the PDB data bank. RN [23] RP X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) OF 20-231 IN COMPLEX WITH RP PHOSPHATE, AND GLYCOSYLATION AT ASN-91. RX PubMed=23196367; DOI=10.1038/embor.2012.189; RA Kesters D., Thompson A.J., Brams M., van Elk R., Spurny R., RA Geitmann M., Villalgordo J.M., Guskov A., Danielson U.H., Lummis S.C., RA Smit A.B., Ulens C.; RT "Structural basis of ligand recognition in 5-HT3 receptors."; RL EMBO Rep. 14:49-56(2013). RN [24] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236. RX PubMed=23695669; RA Stornaiuolo M., De Kloe G.E., Rucktooa P., Fish A., van Elk R., RA Edink E.S., Bertrand D., Smit A.B., de Esch I.J., Sixma T.K.; RT "Assembly of a ?-? stack of ligands in the binding site of an RT acetylcholine-binding protein."; RL Nat. Commun. 4:1875-1875(2013). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF322877; AAL37251.1; -; mRNA. DR RefSeq; NP_001191488.1; NM_001204559.1. DR UniGene; Acl.89; -. DR PDB; 2BR7; X-ray; 3.00 A; A/B/C/D/E=20-236. DR PDB; 2BR8; X-ray; 2.40 A; A/B/C/D/E=20-236. DR PDB; 2BYN; X-ray; 2.02 A; A/B/C/D/E=18-236. DR PDB; 2BYP; X-ray; 2.07 A; A/B/C/D/E=18-225. DR PDB; 2BYQ; X-ray; 3.40 A; A/B/C/D/E=18-236. DR PDB; 2BYR; X-ray; 2.45 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2BYS; X-ray; 2.05 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2C9T; X-ray; 2.25 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2PGZ; X-ray; 1.76 A; A/B/C/D/E=18-236. DR PDB; 2PH9; X-ray; 2.88 A; A/B/C/D/E=18-236. DR PDB; 2UZ6; X-ray; 2.40 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2W8F; X-ray; 2.70 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2W8G; X-ray; 2.60 A; A/B/C/D/E=20-236. DR PDB; 2WN9; X-ray; 1.75 A; A/B/C/D/E=18-236. DR PDB; 2WNC; X-ray; 2.20 A; A/B/C/D/E=18-236. DR PDB; 2WNJ; X-ray; 1.80 A; A/B/C/D/E=18-236. DR PDB; 2WNL; X-ray; 2.70 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2WZY; X-ray; 2.51 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2X00; X-ray; 2.40 A; A/B/C/D/E=18-236. DR PDB; 2XNT; X-ray; 3.21 A; A/B/C/D/E/F/G/H/I/J=1-236. DR PDB; 2XNU; X-ray; 2.55 A; A/B/C/D/E=1-236. DR PDB; 2XNV; X-ray; 2.44 A; A/B/C/D/E/F/G/H/I/J=1-236. DR PDB; 2XYS; X-ray; 1.91 A; A/B/C/D/E=20-236. DR PDB; 2XYT; X-ray; 2.05 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2XZ5; X-ray; 2.80 A; A/B/C/D/E=20-236. DR PDB; 2XZ6; X-ray; 3.14 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2Y54; X-ray; 3.65 A; A/B/C/D/E=20-236. DR PDB; 2Y56; X-ray; 3.59 A; A/B/C/D/E=20-236. DR PDB; 2Y57; X-ray; 3.30 A; A/B/C/D/E=20-236. DR PDB; 2Y58; X-ray; 3.25 A; A/B/C/D/E=20-236. DR PDB; 2Y7Y; X-ray; 1.90 A; A/B/C/D/E=20-236. DR PDB; 2YMD; X-ray; 1.96 A; A/B/C/D/E/F/G/H/I/J=20-231. DR PDB; 2YME; X-ray; 2.40 A; A/B/C/D/E/F/G/H/I/J=20-224. DR PDB; 3C79; X-ray; 2.48 A; A/B/C/D/E=18-236. DR PDB; 3C84; X-ray; 1.94 A; A/B/C/D/E=18-236. DR PDB; 3GUA; X-ray; 3.10 A; A/B/C/D/E/F/G/H/I/J=18-225. DR PDB; 3PEO; X-ray; 2.10 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 3PMZ; X-ray; 2.44 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 3SH1; X-ray; 2.90 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 3SIO; X-ray; 2.32 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 3T4M; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 4AFT; X-ray; 3.20 A; A/B/C/D/E=20-236. DR PDB; 4BFQ; X-ray; 2.40 A; A/B/C/D/E=20-236. DR PDB; 4BQT; X-ray; 2.88 A; A/B/C/D/E=20-236. DR PDB; 4DBM; X-ray; 2.30 A; A/B/C/D/E=18-236. DR PDB; 4EZ1; X-ray; 2.49 A; A/B/C/D/E=18-236. DR PDB; 4FRR; X-ray; 2.20 A; A/B/C/D/E/F/G/H/I/J=18-225. DR PDBsum; 2BR7; -. DR PDBsum; 2BR8; -. DR PDBsum; 2BYN; -. DR PDBsum; 2BYP; -. DR PDBsum; 2BYQ; -. DR PDBsum; 2BYR; -. DR PDBsum; 2BYS; -. DR PDBsum; 2C9T; -. DR PDBsum; 2PGZ; -. DR PDBsum; 2PH9; -. DR PDBsum; 2UZ6; -. DR PDBsum; 2W8F; -. DR PDBsum; 2W8G; -. DR PDBsum; 2WN9; -. DR PDBsum; 2WNC; -. DR PDBsum; 2WNJ; -. DR PDBsum; 2WNL; -. DR PDBsum; 2WZY; -. DR PDBsum; 2X00; -. DR PDBsum; 2XNT; -. DR PDBsum; 2XNU; -. DR PDBsum; 2XNV; -. DR PDBsum; 2XYS; -. DR PDBsum; 2XYT; -. DR PDBsum; 2XZ5; -. DR PDBsum; 2XZ6; -. DR PDBsum; 2Y54; -. DR PDBsum; 2Y56; -. DR PDBsum; 2Y57; -. DR PDBsum; 2Y58; -. DR PDBsum; 2Y7Y; -. DR PDBsum; 2YMD; -. DR PDBsum; 2YME; -. DR PDBsum; 3C79; -. DR PDBsum; 3C84; -. DR PDBsum; 3GUA; -. DR PDBsum; 3PEO; -. DR PDBsum; 3PMZ; -. DR PDBsum; 3SH1; -. DR PDBsum; 3SIO; -. DR PDBsum; 3T4M; -. DR PDBsum; 4AFT; -. DR PDBsum; 4BFQ; -. DR PDBsum; 4BQT; -. DR PDBsum; 4DBM; -. DR PDBsum; 4EZ1; -. DR PDBsum; 4FRR; -. DR ProteinModelPortal; Q8WSF8; -. DR SMR; Q8WSF8; 18-226. DR DIP; DIP-46103N; -. DR MINT; MINT-7300724; -. DR ChEMBL; CHEMBL1944497; -. DR GeneID; 100533247; -. DR EvolutionaryTrace; Q8WSF8; -. DR GO; GO:0016021; C:integral component of membrane; IEA:InterPro. DR GO; GO:0005230; F:extracellular ligand-gated ion channel activity; IEA:InterPro. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR Gene3D; 2.70.170.10; -; 1. DR InterPro; IPR006202; Neur_chan_lig-bd. DR InterPro; IPR006201; Neur_channel. DR PANTHER; PTHR18945; PTHR18945; 1. DR Pfam; PF02931; Neur_chan_LBD; 1. DR SUPFAM; SSF63712; SSF63712; 1. PE 1: Evidence at protein level; KW 3D-structure; Magnesium; Metal-binding; Receptor. FT REGION 167 170 Phosphate 1 binding. FT REGION 167 170 Phosphate 2 binding. FT METAL 167 167 Magnesium 1{EI38}. FT METAL 167 167 Magnesium 2{EI38}. FT METAL 170 170 Magnesium 2{EI38}. FT METAL 210 210 Magnesium 1{EI38}. FT BINDING 42 42 Phosphate 1{EI45}. FT BINDING 42 42 Phosphate 2{EI45}. FT BINDING 59 59 Phosphate 3. FT BINDING 59 59 Phosphate 4. FT BINDING 59 59 Phosphate 5. FT BINDING 68 68 Phosphate 3. FT BINDING 76 76 Phosphate 6{EI46}. FT BINDING 96 96 Phosphate 1{EI45}. FT BINDING 96 96 Phosphate 7{EI45}. FT BINDING 110 110 Cocaine{EI2}. FT BINDING 114 114 Phosphate 3. FT BINDING 114 114 Phosphate 4. FT BINDING 114 114 Phosphate 8. FT BINDING 122 122 Chloride 2; via amide nitrogen. FT BINDING 141 141 Phosphate 3. FT BINDING 164 164 Cocaine{EI2}. FT BINDING 205 205 Cocaine{EI2}. FT BINDING 210 210 Phosphate 1{EI45}. FT BINDING 210 210 Phosphate 2{EI45}. FT BINDING 212 212 Cocaine{EI2}. FT CARBOHYD 91 91 N-linked (GlcNAc...). SQ SEQUENCE 236 AA; 26568 MW; 6D0DE487C46707BE CRC64; MLVSVYLALL VACVGQAHSQ ANLMRLKSDL FNRSPMYPGP TKDDPLTVTL GFTLQDIVKA DSSTNEVDLV YYEQQRWKLN SLMWDPNEYG NITDFRTSAA DIWTPDITAY SSTRPVQVLS PQIAVVTHDG SVMFIPAQRL SFMCDPTGVD SEEGATCAVK FGSWVYSGFE IDLKTDTDQV DLSSYYASSK YEILSATQTR QVQHYSCCPE PYIDVNLVVK FRERRAGNGF FRNLFD //