ID Q8WSF8_APLCA Unreviewed; 236 AA. AC Q8WSF8; DT 01-MAR-2002, integrated into UniProtKB/TrEMBL. DT 01-MAR-2002, sequence version 1. DT 18-SEP-2013, entry version 60. DE SubName: Full=Soluble acetylcholine receptor; OS Aplysia californica (California sea hare). OC Eukaryota; Metazoa; Lophotrochozoa; Mollusca; Gastropoda; OC Heterobranchia; Euthyneura; Euopisthobranchia; Aplysiomorpha; OC Aplysioidea; Aplysiidae; Aplysia. OX NCBI_TaxID=6500; RN [1] RP X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 18-236 IN COMPLEX WITH RP TETRAETHYLENE GLYCOL, AND GLYCOSYLATION AT ASN-91. RX PubMed=16193063; DOI=10.1038/sj.emboj.7600828; RA Hansen S.B., Sulzenbacher G., Huxford T., Marchot P., Taylor P., RA Bourne Y.; RT "Structures of Aplysia AChBP complexes with nicotinic agonists and RT antagonists reveal distinctive binding interfaces and conformations."; RL EMBO J. 24:3635-3646(2005). RN [2] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236 IN COMPLEX WITH RP SULFATE. RX PubMed=15951818; DOI=10.1038/nsmb951; RA Celie P.H., Kasheverov I.E., Mordvintsev D.Y., Hogg R.C., RA van Nierop P., van Elk R., van Rossum-Fikkert S.E., Zhmak M.N., RA Bertrand D., Tsetlin V., Sixma T.K., Smit A.B.; RT "Crystal structure of nicotinic acetylcholine receptor homolog AChBP RT in complex with an alpha-conotoxin PnIA variant."; RL Nat. Struct. Mol. Biol. 12:582-588(2005). RN [3] RP NUCLEOTIDE SEQUENCE. RX PubMed=17190607; DOI=10.1016/j.cell.2006.09.052; RA Moroz L.L., Edwards J.R., Puthanveettil S.V., Kohn A.B., Ha T., RA Heyland A., Knudsen B., Sahni A., Yu F., Liu L., Jezzini S., RA Lovell P., Iannucculli W., Chen M., Nguyen T., Sheng H., Shaw R., RA Kalachikov S., Panchin Y.V., Farmerie W., Russo J.J., Ju J., RA Kandel E.R.; RT "Neuronal transcriptome of Aplysia: neuronal compartments and RT circuitry."; RL Cell 127:1453-1467(2006). RN [4] RP X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 20-236. RX PubMed=16505382; DOI=10.1073/pnas.0507889103; RA Ulens C., Hogg R.C., Celie P.H., Bertrand D., Tsetlin V., Smit A.B., RA Sixma T.K.; RT "Structural determinants of selective alpha-conotoxin binding to a RT nicotinic acetylcholine receptor homolog AChBP."; RL Proc. Natl. Acad. Sci. U.S.A. 103:3615-3620(2006). RN [5] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236. RX PubMed=17660751; DOI=10.1038/sj.emboj.7601785; RA Dutertre S., Ulens C., Buttner R., Fish A., van Elk R., Kendel Y., RA Hopping G., Alewood P.F., Schroeder C., Nicke A., Smit A.B., RA Sixma T.K., Lewis R.J.; RT "AChBP-targeted alpha-conotoxin correlates distinct binding RT orientations with nAChR subtype selectivity."; RL EMBO J. 26:3858-3867(2007). RN [6] RP X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 18-236 IN COMPLEX WITH RP COCAINE AND TETRAETHYLENE GLYCOL, AND GLYCOSYLATION AT ASN-91. RX PubMed=17481657; DOI=10.1016/j.jmb.2007.03.067; RA Hansen S.B., Taylor P.; RT "Galanthamine and non-competitive inhibitor binding to ACh-binding RT protein: evidence for a binding site on non-alpha-subunit interfaces RT of heteromeric neuronal nicotinic receptors."; RL J. Mol. Biol. 369:895-901(2007). RN [7] RP X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 18-225 IN COMPLEX WITH RP SULFATE. RX PubMed=18940802; DOI=10.1074/jbc.C800194200; RA Hansen S.B., Wang H.L., Taylor P., Sine S.M.; RT "An ion selectivity filter in the extracellular domain of Cys-loop RT receptors reveals determinants for ion conductance."; RL J. Biol. Chem. 283:36066-36070(2008). RN [8] RP X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 18-236. RX PubMed=18477694; DOI=10.1073/pnas.0802197105; RA Talley T.T., Harel M., Hibbs R.E., Radic Z., Tomizawa M., Casida J.E., RA Taylor P.; RT "Atomic interactions of neonicotinoid agonists with AChBP: molecular RT recognition of the distinctive electronegative pharmacophore."; RL Proc. Natl. Acad. Sci. U.S.A. 105:7606-7611(2008). RN [9] RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 18-236, AND GLYCOSYLATION AT RP ASN-91. RX PubMed=19696737; DOI=10.1038/emboj.2009.227; RA Hibbs R.E., Sulzenbacher G., Shi J., Talley T.T., Conrod S., Kem W.R., RA Taylor P., Marchot P., Bourne Y.; RT "Structural determinants for interaction of partial agonists with RT acetylcholine binding protein and neuronal alpha7 nicotinic RT acetylcholine receptor."; RL EMBO J. 28:3040-3051(2009). RN [10] RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 20-236. RX PubMed=19331415; DOI=10.1021/jm801400g; RA Ulens C., Akdemir A., Jongejan A., van Elk R., Bertrand S., RA Perrakis A., Leurs R., Smit A.B., Sixma T.K., Bertrand D., RA de Esch I.J.; RT "Use of acetylcholine binding protein in the search for novel alpha7 RT nicotinic receptor ligands. In silico docking, pharmacological RT screening, and X-ray analysis."; RL J. Med. Chem. 52:2372-2383(2009). RN [11] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 18-236. RX PubMed=20224036; DOI=10.1073/pnas.0912372107; RA Bourne Y., Radic Z., Araoz R., Talley T.T., Benoit E., Servent D., RA Taylor P., Molgo J., Marchot P.; RT "Structural determinants in phycotoxins and AChBP conferring high RT affinity binding and nicotinic AChR antagonism."; RL Proc. Natl. Acad. Sci. U.S.A. 107:6076-6081(2010). RN [12] RP X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 18-236. RA Talley T.T., Harel M., Yamauchi G.J., Radic Z., Hansen S., Huxford T., RA Taylor P.W.; RT "The curare alkaloids: analyzing the poses of complexes with the RT acetylcholine binding protein in relation to structure and binding RT energetics."; RL Submitted (OCT-2010) to the PDB data bank. RN [13] RP X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS) OF 18-236. RA Talley T.T., Harel M., Yamauchi J.G., Radic Z., Hansen S., Huxford T., RA Taylor P.W.; RT "The Curare Alkaloids: Analyzing the Poses of Complexes with the RT Acetylcholine Binding Protein in Relation to Structure and Binding RT Energies."; RL Submitted (NOV-2010) to the PDB data bank. RN [14] RP X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS). RX PubMed=21920761; DOI=10.1016/j.bmc.2011.08.028; RA Akdemir A., Rucktooa P., Jongejan A., Elk R.v., Bertrand S., RA Sixma T.K., Bertrand D., Smit A.B., Leurs R., de Graaf C., RA de Esch I.J.; RT "Acetylcholine binding protein (AChBP) as template for hierarchical in RT silico screening procedures to identify structurally novel ligands for RT the nicotinic receptors."; RL Bioorg. Med. Chem. 19:6107-6119(2011). RN [15] RP X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 20-236 IN COMPLEX WITH RP CHLORIDE AND SULFATE. RX PubMed=21322593; DOI=10.1021/ja110571r; RA Edink E., Rucktooa P., Retra K., Akdemir A., Nahar T., Zuiderveld O., RA van Elk R., Janssen E., van Nierop P., van Muijlwijk-Koezen J., RA Smit A.B., Sixma T.K., Leurs R., de Esch I.J.; RT "Fragment growing induces conformational changes in acetylcholine- RT binding protein: a structural and thermodynamic analysis."; RL J. Am. Chem. Soc. 133:5363-5371(2011). RN [16] RP X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 20-236 IN COMPLEX WITH RP PHOSPHATE. RX PubMed=21115477; DOI=10.1074/jbc.M110.188730; RA Brams M., Gay E.A., Saez J.C., Guskov A., van Elk R., RA van der Schors R.C., Peigneur S., Tytgat J., Strelkov S.V., Smit A.B., RA Yakel J.L., Ulens C.; RT "Crystal structures of a cysteine-modified mutant in loop D of RT acetylcholine-binding protein."; RL J. Biol. Chem. 286:4420-4428(2011). RN [17] RP X-RAY CRYSTALLOGRAPHY (2.32 ANGSTROMS) OF 18-236. RX PubMed=22009746; DOI=10.1074/jbc.M111.286583; RA Nemecz A., Taylor P.; RT "Creating an ?7 nicotinic acetylcholine recognition domain from the RT acetylcholine-binding protein: crystallographic and ligand selectivity RT analyses."; RL J. Biol. Chem. 286:42555-42565(2011). RN [18] RP X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS) OF 20-236. RX PubMed=21468359; DOI=10.1371/journal.pbio.1001034; RA Brams M., Pandya A., Kuzmin D., van Elk R., Krijnen L., Yakel J.L., RA Tsetlin V., Smit A.B., Ulens C.; RT "A structural and mutagenic blueprint for molecular recognition of RT strychnine and d-tubocurarine by different cys-loop receptors."; RL PLoS Biol. 9:e1001034-e1001034(2011). RN [19] RP X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 18-236. RX PubMed=22394239; DOI=10.1021/ja3001858; RA Grimster N.P., Stump B., Fotsing J.R., Weide T., Talley T.T., RA Yamauchi J.G., Nemecz A., Kim C., Ho K.Y., Sharpless K.B., Taylor P., RA Fokin V.V.; RT "Generation of candidate ligands for nicotinic acetylcholine receptors RT via in situ click chemistry with a soluble acetylcholine binding RT protein template."; RL J. Am. Chem. Soc. 134:6732-6740(2012). RN [20] RP X-RAY CRYSTALLOGRAPHY (2.88 ANGSTROMS) OF 20-236. RX PubMed=22553201; DOI=10.1074/jbc.M112.360347; RA Rucktooa P., Haseler C.A., van Elk R., Smit A.B., Gallagher T., RA Sixma T.K.; RT "Structural characterization of binding mode of smoking cessation RT drugs to nicotinic acetylcholine receptors through study of ligand RT complexes with acetylcholine-binding protein."; RL J. Biol. Chem. 287:23283-23293(2012). RN [21] RP X-RAY CRYSTALLOGRAPHY (2.49 ANGSTROMS) OF 18-236. RA Filchakova O.M., Talley T.T., Reger A.S., Kim C., Ho K., Han K., RA Taylor P., McIntosh J.M.; RT "Pairwise interaction of alpha-conotoxin BuIA Pro6 with the beta RT subunit of nicotinic acetylcholine receptor."; RL Submitted (MAY-2012) to the PDB data bank. RN [22] RP X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) OF 20-231. RX PubMed=23196367; DOI=10.1038/embor.2012.189; RA Kesters D., Thompson A.J., Brams M., van Elk R., Spurny R., RA Geitmann M., Villalgordo J.M., Guskov A., Danielson U.H., Lummis S.C., RA Smit A.B., Ulens C.; RT "Structural basis of ligand recognition in 5-HT3 receptors."; RL EMBO Rep. 14:49-56(2013). RN [23] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236. RX PubMed=23695669; RA Stornaiuolo M., De Kloe G.E., Rucktooa P., Fish A., van Elk R., RA Edink E.S., Bertrand D., Smit A.B., de Esch I.J., Sixma T.K.; RT "Assembly of a ?-? stack of ligands in the binding site of an RT acetylcholine-binding protein."; RL Nat. Commun. 4:1875-1875(2013). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF322877; AAL37251.1; -; mRNA. DR RefSeq; NP_001191488.1; NM_001204559.1. DR UniGene; Acl.89; -. DR PDB; 2BR7; X-ray; 3.00 A; A/B/C/D/E=20-236. DR PDB; 2BR8; X-ray; 2.40 A; A/B/C/D/E=20-236. DR PDB; 2BYN; X-ray; 2.02 A; A/B/C/D/E=18-236. DR PDB; 2BYP; X-ray; 2.07 A; A/B/C/D/E=18-225. DR PDB; 2BYQ; X-ray; 3.40 A; A/B/C/D/E=18-236. DR PDB; 2BYR; X-ray; 2.45 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2BYS; X-ray; 2.05 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2C9T; X-ray; 2.25 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2PGZ; X-ray; 1.76 A; A/B/C/D/E=18-236. DR PDB; 2PH9; X-ray; 2.88 A; A/B/C/D/E=18-236. DR PDB; 2UZ6; X-ray; 2.40 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2W8F; X-ray; 2.70 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2W8G; X-ray; 2.60 A; A/B/C/D/E=20-236. DR PDB; 2WN9; X-ray; 1.75 A; A/B/C/D/E=18-236. DR PDB; 2WNC; X-ray; 2.20 A; A/B/C/D/E=18-236. DR PDB; 2WNJ; X-ray; 1.80 A; A/B/C/D/E=18-236. DR PDB; 2WNL; X-ray; 2.70 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2WZY; X-ray; 2.51 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2X00; X-ray; 2.40 A; A/B/C/D/E=18-236. DR PDB; 2XNT; X-ray; 3.21 A; A/B/C/D/E/F/G/H/I/J=1-236. DR PDB; 2XNU; X-ray; 2.55 A; A/B/C/D/E=1-236. DR PDB; 2XNV; X-ray; 2.44 A; A/B/C/D/E/F/G/H/I/J=1-236. DR PDB; 2XYS; X-ray; 1.91 A; A/B/C/D/E=20-236. DR PDB; 2XYT; X-ray; 2.05 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2XZ5; X-ray; 2.80 A; A/B/C/D/E=20-236. DR PDB; 2XZ6; X-ray; 3.14 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2Y54; X-ray; 3.65 A; A/B/C/D/E=20-236. DR PDB; 2Y56; X-ray; 3.59 A; A/B/C/D/E=20-236. DR PDB; 2Y57; X-ray; 3.30 A; A/B/C/D/E=20-236. DR PDB; 2Y58; X-ray; 3.25 A; A/B/C/D/E=20-236. DR PDB; 2Y7Y; X-ray; 1.90 A; A/B/C/D/E=20-236. DR PDB; 2YMD; X-ray; 1.96 A; A/B/C/D/E/F/G/H/I/J=20-231. DR PDB; 2YME; X-ray; 2.40 A; A/B/C/D/E/F/G/H/I/J=20-224. DR PDB; 3C79; X-ray; 2.48 A; A/B/C/D/E=18-236. DR PDB; 3C84; X-ray; 1.94 A; A/B/C/D/E=18-236. DR PDB; 3GUA; X-ray; 3.10 A; A/B/C/D/E/F/G/H/I/J=18-225. DR PDB; 3PEO; X-ray; 2.10 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 3PMZ; X-ray; 2.44 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 3SH1; X-ray; 2.90 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 3SIO; X-ray; 2.32 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 3T4M; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 4AFT; X-ray; 3.20 A; A/B/C/D/E=20-236. DR PDB; 4BFQ; X-ray; 2.40 A; A/B/C/D/E=20-236. DR PDB; 4BQT; X-ray; 2.88 A; A/B/C/D/E=20-236. DR PDB; 4DBM; X-ray; 2.30 A; A/B/C/D/E=18-236. DR PDB; 4EZ1; X-ray; 2.49 A; A/B/C/D/E=18-236. DR PDBsum; 2BR7; -. DR PDBsum; 2BR8; -. DR PDBsum; 2BYN; -. DR PDBsum; 2BYP; -. DR PDBsum; 2BYQ; -. DR PDBsum; 2BYR; -. DR PDBsum; 2BYS; -. DR PDBsum; 2C9T; -. DR PDBsum; 2PGZ; -. DR PDBsum; 2PH9; -. DR PDBsum; 2UZ6; -. DR PDBsum; 2W8F; -. DR PDBsum; 2W8G; -. DR PDBsum; 2WN9; -. DR PDBsum; 2WNC; -. DR PDBsum; 2WNJ; -. DR PDBsum; 2WNL; -. DR PDBsum; 2WZY; -. DR PDBsum; 2X00; -. DR PDBsum; 2XNT; -. DR PDBsum; 2XNU; -. DR PDBsum; 2XNV; -. DR PDBsum; 2XYS; -. DR PDBsum; 2XYT; -. DR PDBsum; 2XZ5; -. DR PDBsum; 2XZ6; -. DR PDBsum; 2Y54; -. DR PDBsum; 2Y56; -. DR PDBsum; 2Y57; -. DR PDBsum; 2Y58; -. DR PDBsum; 2Y7Y; -. DR PDBsum; 2YMD; -. DR PDBsum; 2YME; -. DR PDBsum; 3C79; -. DR PDBsum; 3C84; -. DR PDBsum; 3GUA; -. DR PDBsum; 3PEO; -. DR PDBsum; 3PMZ; -. DR PDBsum; 3SH1; -. DR PDBsum; 3SIO; -. DR PDBsum; 3T4M; -. DR PDBsum; 4AFT; -. DR PDBsum; 4BFQ; -. DR PDBsum; 4BQT; -. DR PDBsum; 4DBM; -. DR PDBsum; 4EZ1; -. DR ProteinModelPortal; Q8WSF8; -. DR SMR; Q8WSF8; 18-226. DR DIP; DIP-46103N; -. DR MINT; MINT-7300724; -. DR GeneID; 100533247; -. DR ChEMBL; CHEMBL1944497; -. DR EvolutionaryTrace; Q8WSF8; -. DR GO; GO:0016021; C:integral to membrane; IEA:InterPro. DR GO; GO:0005230; F:extracellular ligand-gated ion channel activity; IEA:InterPro. DR Gene3D; 2.70.170.10; -; 1. DR InterPro; IPR006202; Neur_chan_lig-bd. DR InterPro; IPR006201; Neur_channel. DR PANTHER; PTHR18945; PTHR18945; 1. DR Pfam; PF02931; Neur_chan_LBD; 1. DR SUPFAM; SSF63712; Neur_chan_LBD; 1. PE 1: Evidence at protein level; KW 3D-structure; Chloride; Receptor. FT REGION 20 22 Sulfate 1 binding. FT REGION 78 80 Sulfate 4 binding. FT REGION 94 96 Sulfate 2 binding. FT REGION 94 96 Sulfate 8 binding. FT REGION 167 170 Sulfate 3 binding. FT REGION 186 188 Sulfate 17 binding. FT REGION 186 188 Sulfate 18 binding. FT REGION 201 203 Sulfate 13 binding. FT BINDING 42 42 Sulfate 3. FT BINDING 55 55 Sulfate 5. FT BINDING 59 59 Phosphate 1. FT BINDING 59 59 Phosphate 2. FT BINDING 59 59 Phosphate 3. FT BINDING 59 59 Sulfate 6. FT BINDING 59 59 Sulfate 7. FT BINDING 68 68 Phosphate 1. FT BINDING 103 103 Sulfate 9. FT BINDING 110 110 Cocaine. FT BINDING 114 114 Phosphate 1. FT BINDING 114 114 Phosphate 2. FT BINDING 114 114 Phosphate 4. FT BINDING 114 114 Sulfate 6. FT BINDING 122 122 Chloride 2; via amide nitrogen. FT BINDING 122 122 Sulfate 10; via amide nitrogen. FT BINDING 122 122 Sulfate 11; via amide nitrogen. FT BINDING 141 141 Phosphate 1. FT BINDING 145 145 Sulfate 11; via amide nitrogen. FT BINDING 145 145 Sulfate 12; via amide nitrogen. FT BINDING 145 145 Sulfate 13; via amide nitrogen. FT BINDING 145 145 Sulfate 14; via amide nitrogen. FT BINDING 145 145 Sulfate 15; via amide nitrogen. FT BINDING 145 145 Sulfate 16; via amide nitrogen. FT BINDING 145 145 Sulfate 17; via amide nitrogen. FT BINDING 145 145 Sulfate 1; via amide nitrogen. FT BINDING 160 160 Sulfate 13. FT BINDING 160 160 Sulfate 15. FT BINDING 164 164 Cocaine. FT BINDING 164 164 Tetraethylene glycol 1. FT BINDING 164 164 Tetraethylene glycol 3. FT BINDING 166 166 Sulfate 9. FT BINDING 170 170 Sulfate 2. FT BINDING 184 184 Sulfate 5; via carbonyl oxygen. FT BINDING 201 201 Sulfate 15. FT BINDING 203 203 Sulfate 17. FT BINDING 205 205 Cocaine. FT BINDING 207 207 Sulfate 19; via amide nitrogen. FT BINDING 207 207 Sulfate 20. FT BINDING 212 212 Cocaine. FT BINDING 212 212 Tetraethylene glycol 3. FT BINDING 216 216 Sulfate 17. FT CARBOHYD 91 91 N-linked (GlcNAc...). SQ SEQUENCE 236 AA; 26568 MW; 6D0DE487C46707BE CRC64; MLVSVYLALL VACVGQAHSQ ANLMRLKSDL FNRSPMYPGP TKDDPLTVTL GFTLQDIVKA DSSTNEVDLV YYEQQRWKLN SLMWDPNEYG NITDFRTSAA DIWTPDITAY SSTRPVQVLS PQIAVVTHDG SVMFIPAQRL SFMCDPTGVD SEEGATCAVK FGSWVYSGFE IDLKTDTDQV DLSSYYASSK YEILSATQTR QVQHYSCCPE PYIDVNLVVK FRERRAGNGF FRNLFD //