ID Q8WSF8_APLCA Unreviewed; 236 AA. AC Q8WSF8; DT 01-MAR-2002, integrated into UniProtKB/TrEMBL. DT 01-MAR-2002, sequence version 1. DT 03-MAY-2011, entry version 38. DE SubName: Full=Soluble acetylcholine receptor; OS Aplysia californica (California sea hare). OC Eukaryota; Metazoa; Mollusca; Gastropoda; Heterobranchia; Euthyneura; OC Euopisthobranchia; Aplysiomorpha; Aplysioidea; Aplysiidae; Aplysia. OX NCBI_TaxID=6500; RN [1] RP NUCLEOTIDE SEQUENCE. RX PubMed=17190607; DOI=10.1016/j.cell.2006.09.052; RA Moroz L.L., Edwards J.R., Puthanveettil S.V., Kohn A.B., Ha T., RA Heyland A., Knudsen B., Sahni A., Yu F., Liu L., Jezzini S., RA Lovell P., Iannucculli W., Chen M., Nguyen T., Sheng H., Shaw R., RA Kalachikov S., Panchin Y.V., Farmerie W., Russo J.J., Ju J., RA Kandel E.R.; RT "Neuronal transcriptome of aplysia: neuronal compartments and RT circuitry."; RL Cell 127:1453-1467(2006). RN [2] RP NUCLEOTIDE SEQUENCE. RA Sadreyev R.I., Panchin Y.V., Meleshkevich E.A., Moroz L.L.; RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases. RN [3] RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 20-236. RX PubMed=19331415; DOI=10.1021/jm801400g; RA Ulens C., Akdemir A., Jongejan A., van Elk R., Bertrand S., RA Perrakis A., Leurs R., Smit A.B., Sixma T.K., Bertrand D., RA de Esch I.J.; RT "Use of acetylcholine binding protein in the search for novel alpha7 RT nicotinic receptor ligands. In silico docking, pharmacological RT screening, and X-ray analysis."; RL J. Med. Chem. 52:2372-2383(2009). RN [4] RP X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 18-236 IN COMPLEX WITH RP COCAINE, AND GLYCOSYLATION AT ASN-91. RX PubMed=17481657; DOI=10.1016/j.jmb.2007.03.067; RA Hansen S.B., Taylor P.; RT "Galanthamine and non-competitive inhibitor binding to ACh-binding RT protein: evidence for a binding site on non-alpha-subunit interfaces RT of heteromeric neuronal nicotinic receptors."; RL J. Mol. Biol. 369:895-901(2007). RN [5] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236. RX PubMed=15951818; DOI=10.1038/nsmb951; RA Celie P.H., Kasheverov I.E., Mordvintsev D.Y., Hogg R.C., RA van Nierop P., van Elk R., van Rossum-Fikkert S.E., Zhmak M.N., RA Bertrand D., Tsetlin V., Sixma T.K., Smit A.B.; RT "Crystal structure of nicotinic acetylcholine receptor homolog AChBP RT in complex with an alpha-conotoxin PnIA variant."; RL Nat. Struct. Mol. Biol. 12:582-588(2005). RN [6] RP X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 18-236. RX PubMed=18477694; DOI=10.1073/pnas.0802197105; RA Talley T.T., Harel M., Hibbs R.E., Radic Z., Tomizawa M., Casida J.E., RA Taylor P.; RT "Atomic interactions of neonicotinoid agonists with AChBP: molecular RT recognition of the distinctive electronegative pharmacophore."; RL Proc. Natl. Acad. Sci. U.S.A. 105:7606-7611(2008). RN [7] RP X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 20-236. RX PubMed=16505382; DOI=10.1073/pnas.0507889103; RA Ulens C., Hogg R.C., Celie P.H., Bertrand D., Tsetlin V., Smit A.B., RA Sixma T.K.; RT "Structural determinants of selective alpha-conotoxin binding to a RT nicotinic acetylcholine receptor homolog AChBP."; RL Proc. Natl. Acad. Sci. U.S.A. 103:3615-3620(2006). RN [8] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 18-236. RX PubMed=20224036; DOI=10.1073/pnas.0912372107; RA Bourne Y., Radic Z., Araoz R., Talley T.T., Benoit E., Servent D., RA Taylor P., Molgo J., Marchot P.; RT "Structural determinants in phycotoxins and AChBP conferring high RT affinity binding and nicotinic AChR antagonism."; RL Proc. Natl. Acad. Sci. U.S.A. 107:6076-6081(2010). RN [9] RP X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 18-225. RX PubMed=18940802; DOI=10.1074/jbc.C800194200; RA Hansen S.B., Wang H.L., Taylor P., Sine S.M.; RT "An ion selectivity filter in the extracellular domain of Cys-loop RT receptors reveals determinants for ion conductance."; RL J. Biol. Chem. 283:36066-36070(2008). RN [10] RP X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 18-236, AND GLYCOSYLATION AT RP ASN-91. RX PubMed=16193063; DOI=10.1038/sj.emboj.7600828; RA Hansen S.B., Sulzenbacher G., Huxford T., Marchot P., Taylor P., RA Bourne Y.; RT "Structures of Aplysia AChBP complexes with nicotinic agonists and RT antagonists reveal distinctive binding interfaces and conformations."; RL EMBO J. 24:3635-3646(2005). RN [11] RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236. RX PubMed=17660751; DOI=10.1038/sj.emboj.7601785; RA Dutertre S., Ulens C., Buttner R., Fish A., van Elk R., Kendel Y., RA Hopping G., Alewood P.F., Schroeder C., Nicke A., Smit A.B., RA Sixma T.K., Lewis R.J.; RT "AChBP-targeted alpha-conotoxin correlates distinct binding RT orientations with nAChR subtype selectivity."; RL EMBO J. 26:3858-3867(2007). RN [12] RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 18-236, AND GLYCOSYLATION AT RP ASN-91. RX PubMed=19696737; DOI=10.1038/emboj.2009.227; RA Hibbs R.E., Sulzenbacher G., Shi J., Talley T.T., Conrod S., Kem W.R., RA Taylor P., Marchot P., Bourne Y.; RT "Structural determinants for interaction of partial agonists with RT acetylcholine binding protein and neuronal alpha7 nicotinic RT acetylcholine receptor."; RL EMBO J. 28:3040-3051(2009). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF322877; AAL37251.1; -; mRNA. DR RefSeq; NP_001191488.1; NM_001204559.1. DR PDB; 2BR7; X-ray; 3.00 A; A/B/C/D/E=20-236. DR PDB; 2BR8; X-ray; 2.40 A; A/B/C/D/E=20-236. DR PDB; 2BYN; X-ray; 2.02 A; A/B/C/D/E=18-236. DR PDB; 2BYP; X-ray; 2.07 A; A/B/C/D/E=18-225. DR PDB; 2BYQ; X-ray; 3.40 A; A/B/C/D/E=18-236. DR PDB; 2BYR; X-ray; 2.45 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2BYS; X-ray; 2.05 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2C9T; X-ray; 2.25 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2PGZ; X-ray; 1.76 A; A/B/C/D/E=18-236. DR PDB; 2PH9; X-ray; 2.88 A; A/B/C/D/E=18-236. DR PDB; 2UZ6; X-ray; 2.40 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2W8E; X-ray; 1.90 A; A/B/C/D/E=20-236. DR PDB; 2W8F; X-ray; 2.70 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 2W8G; X-ray; 2.60 A; A/B/C/D/E=20-236. DR PDB; 2WN9; X-ray; 1.75 A; A/B/C/D/E=18-236. DR PDB; 2WNC; X-ray; 2.20 A; A/B/C/D/E=18-236. DR PDB; 2WNJ; X-ray; 1.80 A; A/B/C/D/E=18-236. DR PDB; 2WNL; X-ray; 2.70 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2WZY; X-ray; 2.51 A; A/B/C/D/E/F/G/H/I/J=18-236. DR PDB; 2X00; X-ray; 2.40 A; A/B/C/D/E=18-236. DR PDB; 2XZ5; X-ray; 2.80 A; A/B/C/D/E=20-236. DR PDB; 2XZ6; X-ray; 3.14 A; A/B/C/D/E/F/G/H/I/J=20-236. DR PDB; 3C79; X-ray; 2.48 A; A/B/C/D/E=18-236. DR PDB; 3C84; X-ray; 1.94 A; A/B/C/D/E=18-236. DR PDB; 3GUA; X-ray; 3.10 A; A/B/C/D/E/F/G/H/I/J=18-225. DR PDBsum; 2BR7; -. DR PDBsum; 2BR8; -. DR PDBsum; 2BYN; -. DR PDBsum; 2BYP; -. DR PDBsum; 2BYQ; -. DR PDBsum; 2BYR; -. DR PDBsum; 2BYS; -. DR PDBsum; 2C9T; -. DR PDBsum; 2PGZ; -. DR PDBsum; 2PH9; -. DR PDBsum; 2UZ6; -. DR PDBsum; 2W8E; -. DR PDBsum; 2W8F; -. DR PDBsum; 2W8G; -. DR PDBsum; 2WN9; -. DR PDBsum; 2WNC; -. DR PDBsum; 2WNJ; -. DR PDBsum; 2WNL; -. DR PDBsum; 2WZY; -. DR PDBsum; 2X00; -. DR PDBsum; 2XZ5; -. DR PDBsum; 2XZ6; -. DR PDBsum; 3C79; -. DR PDBsum; 3C84; -. DR PDBsum; 3GUA; -. DR ProteinModelPortal; Q8WSF8; -. DR SMR; Q8WSF8; 18-226. DR DIP; DIP-46103N; -. DR MINT; MINT-7300724; -. DR GeneID; 100533247; -. DR GO; GO:0016021; C:integral to membrane; IEA:InterPro. DR GO; GO:0005230; F:extracellular ligand-gated ion channel activity; IEA:InterPro. DR GO; GO:0004872; F:receptor activity; IEA:UniProtKB-KW. DR InterPro; IPR006202; Neur_chan_lig-bd. DR InterPro; IPR006201; Neur_channel. DR Gene3D; G3DSA:2.70.170.10; Neur_chan_lig_bd; 1. DR PANTHER; PTHR18945; Neur_channel; 1. DR Pfam; PF02931; Neur_chan_LBD; 1. DR SUPFAM; SSF63712; Neur_chan_LBD; 1. PE 1: Evidence at protein level; KW 3D-structure; Receptor. FT REGION 205 208 Cocaine binding. FT BINDING 53 53 Cocaine. FT BINDING 74 74 Cocaine. FT BINDING 110 110 Cocaine. FT BINDING 135 135 Cocaine. FT BINDING 164 164 Cocaine. FT CARBOHYD 91 91 N-linked (GlcNAc...). SQ SEQUENCE 236 AA; 26568 MW; 6D0DE487C46707BE CRC64; MLVSVYLALL VACVGQAHSQ ANLMRLKSDL FNRSPMYPGP TKDDPLTVTL GFTLQDIVKA DSSTNEVDLV YYEQQRWKLN SLMWDPNEYG NITDFRTSAA DIWTPDITAY SSTRPVQVLS PQIAVVTHDG SVMFIPAQRL SFMCDPTGVD SEEGATCAVK FGSWVYSGFE IDLKTDTDQV DLSSYYASSK YEILSATQTR QVQHYSCCPE PYIDVNLVVK FRERRAGNGF FRNLFD //