ID ARL8C_ARATH Reviewed; 184 AA. AC Q8W4C8; Q9FHQ2; DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2002, sequence version 1. DT 24-JAN-2024, entry version 153. DE RecName: Full=ADP-ribosylation factor-like protein 8c {ECO:0000303|PubMed:22174675}; DE Short=AtARL8c {ECO:0000303|PubMed:22174675}; DE AltName: Full=ADP-ribosylation factor-like A1A {ECO:0000303|PubMed:12644670}; DE Short=AtARLA1A {ECO:0000303|PubMed:12644670}; GN Name=ARL8C {ECO:0000303|PubMed:22174675}; GN Synonyms=ARLA1A {ECO:0000303|PubMed:12644670}; GN OrderedLocusNames=At5g37680 {ECO:0000312|Araport:AT5G37680}; GN ORFNames=K12B20.15 {ECO:0000312|EMBL:BAB08314.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10470850; DOI=10.1093/dnares/6.3.183; RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., RA Miyajima N., Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC RT clones."; RL DNA Res. 6:183-195(1999). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., RA Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [5] RP GENE FAMILY, AND NOMENCLATURE. RX PubMed=12644670; DOI=10.1104/pp.013052; RA Vernoud V., Horton A.C., Yang Z., Nielsen E.; RT "Analysis of the small GTPase gene superfamily of Arabidopsis."; RL Plant Physiol. 131:1191-1208(2003). RN [6] RP GENE FAMILY. RX PubMed=15723828; DOI=10.1093/jxb/eri099; RA Gebbie L.K., Burn J.E., Hocart C.H., Williamson R.E.; RT "Genes encoding ADP-ribosylation factors in Arabidopsis thaliana; genome RT analysis and antisense suppression."; RL J. Exp. Bot. 56:1079-1091(2005). RN [7] RP FUNCTION (MICROBIAL INFECTION), DISRUPTION PHENOTYPE, GENE FAMILY, AND RP NOMENCLATURE. RC STRAIN=cv. Columbia; RX PubMed=22174675; DOI=10.1371/journal.ppat.1002409; RA Nishikiori M., Mori M., Dohi K., Okamura H., Katoh E., Naito S., Meshi T., RA Ishikawa M.; RT "A host small GTP-binding protein ARL8 plays crucial roles in tobamovirus RT RNA replication."; RL PLoS Pathog. 7:E1002409-E1002409(2011). CC -!- FUNCTION: May play a role in lysosome motility. May play a role in CC chromosome segregation. {ECO:0000250|UniProtKB:Q9NVJ2}. CC -!- FUNCTION: (Microbial infection) Component of tomato mosaic virus (ToMV) CC RNA replication complexes. Required for tobamovirus multiplication, CC especially for efficient negative-strand RNA synthesis and viral RNA CC capping. {ECO:0000269|PubMed:22174675}. CC -!- SUBUNIT: Interacts with tubulin. {ECO:0000250|UniProtKB:Q9NVJ2}. CC -!- SUBCELLULAR LOCATION: Late endosome membrane CC {ECO:0000250|UniProtKB:Q9NVJ2}. Lysosome membrane CC {ECO:0000250|UniProtKB:Q9NVJ2}. Cytoplasm, cytoskeleton, spindle CC {ECO:0000250|UniProtKB:Q9NVJ2}. Note=Localizes with microtubules at the CC spindle mid-zone during mitosis. {ECO:0000250|UniProtKB:Q9NVJ2}. CC -!- DISRUPTION PHENOTYPE: In the triple mutants arl8a-1 arl8b-1 arl8c-1, CC impaired multiplication of tomato mosaic virus (ToMV). CC {ECO:0000269|PubMed:22174675}. CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family. CC {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=BAB08314.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB018107; BAB08314.1; ALT_SEQ; Genomic_DNA. DR EMBL; CP002688; AED94219.1; -; Genomic_DNA. DR EMBL; AY062658; AAL32736.1; -; mRNA. DR EMBL; AY114643; AAM47962.1; -; mRNA. DR EMBL; AY085271; AAM62503.1; -; mRNA. DR RefSeq; NP_568553.1; NM_123127.4. DR AlphaFoldDB; Q8W4C8; -. DR SMR; Q8W4C8; -. DR IntAct; Q8W4C8; 2. DR STRING; 3702.Q8W4C8; -. DR PaxDb; 3702-AT5G37680-1; -. DR ProteomicsDB; 246669; -. DR EnsemblPlants; AT5G37680.1; AT5G37680.1; AT5G37680. DR GeneID; 833747; -. DR Gramene; AT5G37680.1; AT5G37680.1; AT5G37680. DR KEGG; ath:AT5G37680; -. DR Araport; AT5G37680; -. DR TAIR; AT5G37680; ARLA1A. DR eggNOG; KOG0075; Eukaryota. DR HOGENOM; CLU_040729_10_1_1; -. DR InParanoid; Q8W4C8; -. DR OMA; RFRSEWG; -. DR OrthoDB; 5349301at2759; -. DR PhylomeDB; Q8W4C8; -. DR PRO; PR:Q8W4C8; -. DR Proteomes; UP000006548; Chromosome 5. DR ExpressionAtlas; Q8W4C8; baseline and differential. DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell. DR GO; GO:0009536; C:plastid; HDA:TAIR. DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell. DR GO; GO:0005525; F:GTP binding; ISS:TAIR. DR GO; GO:0003924; F:GTPase activity; IEA:InterPro. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW. DR GO; GO:0051607; P:defense response to virus; IGI:TAIR. DR GO; GO:0015031; P:protein transport; IEA:InterPro. DR CDD; cd04159; Arl10_like; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1. DR InterPro; IPR044154; Arl8a/8b. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR005225; Small_GTP-bd_dom. DR InterPro; IPR006689; Small_GTPase_ARF/SAR. DR NCBIfam; TIGR00231; small_GTP; 1. DR PANTHER; PTHR45732; ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8; 1. DR PANTHER; PTHR45732:SF12; ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8C; 1. DR Pfam; PF00025; Arf; 1. DR PRINTS; PR00328; SAR1GTPBP. DR SMART; SM00177; ARF; 1. DR SMART; SM00175; RAB; 1. DR SMART; SM00173; RAS; 1. DR SMART; SM00178; SAR; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR PROSITE; PS51417; ARF; 1. PE 2: Evidence at transcript level; KW Cell cycle; Cell division; Chromosome partition; Cytoplasm; Cytoskeleton; KW Endosome; GTP-binding; Lysosome; Membrane; Mitosis; Nucleotide-binding; KW Reference proteome. FT CHAIN 1..184 FT /note="ADP-ribosylation factor-like protein 8c" FT /id="PRO_0000438005" FT INTRAMEM 1..18 FT /note="Note=Mediates targeting to membranes" FT /evidence="ECO:0000250|UniProtKB:Q9NVJ2" FT BINDING 29..34 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000250|UniProtKB:P62330" FT BINDING 48..51 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000250|UniProtKB:P62330" FT BINDING 70..74 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000250|UniProtKB:P62330" FT BINDING 129..132 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000250|UniProtKB:P62330" SQ SEQUENCE 184 AA; 20573 MW; D3EE2D625E8C94CC CRC64; MGLWDSLLNW LRSLFFKQEM ELSLVGLQNA GKTSLVNAIA TGGYSEDMIP TVGFNMRKVT KGNVTIKIWD LGGQRRFRTM WERYCRGVSA IVYVIDAADR DSVPISRSEL NDLLTKPSLN GIPLLILGNK IDKSEALSKQ ALVDQLGLES VTDREVCCYM ISCKDSINID AVIDWLIKHS RTAT //