ID ARL8C_ARATH Reviewed; 184 AA. AC Q8W4C8; Q9FHQ2; DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2002, sequence version 1. DT 05-JUL-2017, entry version 125. DE RecName: Full=ADP-ribosylation factor-like protein 8c {ECO:0000303|PubMed:22174675}; DE Short=AtARL8c {ECO:0000303|PubMed:22174675}; DE AltName: Full=ADP-ribosylation factor-like A1A {ECO:0000303|PubMed:12644670}; DE Short=AtARLA1A {ECO:0000303|PubMed:12644670}; GN Name=ARL8C {ECO:0000303|PubMed:22174675}; GN Synonyms=ARLA1A {ECO:0000303|PubMed:12644670}; GN OrderedLocusNames=At5g37680 {ECO:0000312|Araport:AT5G37680}; GN ORFNames=K12B20.15 {ECO:0000312|EMBL:BAB08314.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; OC Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10470850; DOI=10.1093/dnares/6.3.183; RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., RA Miyajima N., Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. RT Sequence features of the regions of 1,011,550 bp covered by seventeen RT P1 and TAC clones."; RL DNA Res. 6:183-195(1999). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Portal (Araport); RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [5] RP GENE FAMILY, AND NOMENCLATURE. RX PubMed=12644670; DOI=10.1104/pp.013052; RA Vernoud V., Horton A.C., Yang Z., Nielsen E.; RT "Analysis of the small GTPase gene superfamily of Arabidopsis."; RL Plant Physiol. 131:1191-1208(2003). RN [6] RP GENE FAMILY. RX PubMed=15723828; DOI=10.1093/jxb/eri099; RA Gebbie L.K., Burn J.E., Hocart C.H., Williamson R.E.; RT "Genes encoding ADP-ribosylation factors in Arabidopsis thaliana; RT genome analysis and antisense suppression."; RL J. Exp. Bot. 56:1079-1091(2005). RN [7] RP FUNCTION (MICROBIAL INFECTION), DISRUPTION PHENOTYPE, GENE FAMILY, AND RP NOMENCLATURE. RC STRAIN=cv. Columbia; RX PubMed=22174675; DOI=10.1371/journal.ppat.1002409; RA Nishikiori M., Mori M., Dohi K., Okamura H., Katoh E., Naito S., RA Meshi T., Ishikawa M.; RT "A host small GTP-binding protein ARL8 plays crucial roles in RT tobamovirus RNA replication."; RL PLoS Pathog. 7:E1002409-E1002409(2011). CC -!- FUNCTION: May play a role in lysosome motility. May play a role in CC chromosome segregation. {ECO:0000250|UniProtKB:Q9NVJ2}. CC -!- FUNCTION: (Microbial infection) Component of tomato mosaic virus CC (ToMV) RNA replication complexes. Required for tobamovirus CC multiplication, especially for efficient negative-strand RNA CC synthesis and viral RNA capping. {ECO:0000269|PubMed:22174675}. CC -!- SUBUNIT: Interacts with tubulin. {ECO:0000250|UniProtKB:Q9NVJ2}. CC -!- SUBCELLULAR LOCATION: Late endosome membrane CC {ECO:0000250|UniProtKB:Q9NVJ2}. Lysosome membrane CC {ECO:0000250|UniProtKB:Q9NVJ2}. Cytoplasm, cytoskeleton, spindle CC {ECO:0000250|UniProtKB:Q9NVJ2}. Note=Localizes with microtubules CC at the spindle mid-zone during mitosis. CC {ECO:0000250|UniProtKB:Q9NVJ2}. CC -!- DISRUPTION PHENOTYPE: In the triple mutants arl8a-1 arl8b-1 arl8c- CC 1, impaired multiplication of tomato mosaic virus (ToMV). CC {ECO:0000269|PubMed:22174675}. CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family. CC {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=BAB08314.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB018107; BAB08314.1; ALT_SEQ; Genomic_DNA. DR EMBL; CP002688; AED94219.1; -; Genomic_DNA. DR EMBL; AY062658; AAL32736.1; -; mRNA. DR EMBL; AY114643; AAM47962.1; -; mRNA. DR EMBL; AY085271; AAM62503.1; -; mRNA. DR RefSeq; NP_568553.1; NM_123127.4. DR UniGene; At.27273; -. DR ProteinModelPortal; Q8W4C8; -. DR SMR; Q8W4C8; -. DR IntAct; Q8W4C8; 2. DR STRING; 3702.AT5G37680.1; -. DR PaxDb; Q8W4C8; -. DR EnsemblPlants; AT5G37680.1; AT5G37680.1; AT5G37680. DR GeneID; 833747; -. DR Gramene; AT5G37680.1; AT5G37680.1; AT5G37680. DR KEGG; ath:AT5G37680; -. DR Araport; AT5G37680; -. DR TAIR; locus:2151734; AT5G37680. DR eggNOG; KOG0075; Eukaryota. DR eggNOG; ENOG410XNUK; LUCA. DR HOGENOM; HOG000163692; -. DR KO; K07955; -. DR OMA; MKCWDIG; -. DR OrthoDB; EOG09360NQX; -. DR PhylomeDB; Q8W4C8; -. DR Reactome; R-ATH-6798695; Neutrophil degranulation. DR PRO; PR:Q8W4C8; -. DR Proteomes; UP000006548; Chromosome 5. DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell. DR GO; GO:0005525; F:GTP binding; ISS:TAIR. DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW. DR GO; GO:0051607; P:defense response to virus; IGI:TAIR. DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR005225; Small_GTP-bd_dom. DR InterPro; IPR024156; Small_GTPase_ARF. DR InterPro; IPR006689; Small_GTPase_ARF/SAR. DR Pfam; PF00025; Arf; 1. DR PRINTS; PR00328; SAR1GTPBP. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR00231; small_GTP; 1. DR PROSITE; PS51417; ARF; 1. PE 2: Evidence at transcript level; KW Cell cycle; Cell division; Chromosome partition; Complete proteome; KW Cytoplasm; Cytoskeleton; Endosome; GTP-binding; Lysosome; Membrane; KW Mitosis; Nucleotide-binding; Reference proteome. FT CHAIN 1 184 ADP-ribosylation factor-like protein 8c. FT /FTId=PRO_0000438005. FT INTRAMEM 1 18 Note=Mediates targeting to membranes. FT {ECO:0000250|UniProtKB:Q9NVJ2}. FT NP_BIND 29 34 GTP. {ECO:0000250|UniProtKB:P62330}. FT NP_BIND 48 51 GTP. {ECO:0000250|UniProtKB:P62330}. FT NP_BIND 70 74 GTP. {ECO:0000250|UniProtKB:P62330}. FT NP_BIND 129 132 GTP. {ECO:0000250|UniProtKB:P62330}. SQ SEQUENCE 184 AA; 20573 MW; D3EE2D625E8C94CC CRC64; MGLWDSLLNW LRSLFFKQEM ELSLVGLQNA GKTSLVNAIA TGGYSEDMIP TVGFNMRKVT KGNVTIKIWD LGGQRRFRTM WERYCRGVSA IVYVIDAADR DSVPISRSEL NDLLTKPSLN GIPLLILGNK IDKSEALSKQ ALVDQLGLES VTDREVCCYM ISCKDSINID AVIDWLIKHS RTAT //