ID MCU2_ARATH Reviewed; 293 AA. AC Q8VYR0; Q9FVU1; DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2002, sequence version 1. DT 02-OCT-2024, entry version 123. DE RecName: Full=Calcium uniporter protein 2, mitochondrial; DE Short=AtMCU2 {ECO:0000303|PubMed:28031475}; DE Flags: Precursor; GN Name=MCU2 {ECO:0000303|PubMed:28031475}; GN OrderedLocusNames=At1g57610 {ECO:0000312|Araport:AT1G57610}; GN ORFNames=T8L23.8 {ECO:0000312|EMBL:AAG50752.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAL49777.1}; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., RA Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."; RL Nature 408:816-820(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., RA Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [4] RP GENE FAMILY, AND REVIEW. RX PubMed=22200666; DOI=10.1093/jxb/err394; RA Stael S., Wurzinger B., Mair A., Mehlmer N., Vothknecht U.C., Teige M.; RT "Plant organellar calcium signalling: an emerging field."; RL J. Exp. Bot. 63:1525-1542(2012). RN [5] RP NOMENCLATURE. RX PubMed=28031475; DOI=10.1104/pp.16.01359; RA Teardo E., Carraretto L., Wagner S., Formentin E., Behera S., RA De Bortoli S., Larosa V., Fuchs P., Lo Schiavo F., Raffaello A., RA Rizzuto R., Costa A., Schwarzlaender M., Szabo I.; RT "Physiological Characterization of a Plant Mitochondrial Calcium Uniporter RT in Vitro and in Vivo."; RL Plant Physiol. 173:1355-1370(2017). CC -!- FUNCTION: Mitochondrial inner membrane calcium uniporter that mediates CC calcium uptake into mitochondria (By similarity). Constitutes a pore- CC forming and calcium-conducting subunit (By similarity). Mitochondrial CC calcium homeostasis plays key roles in cellular physiology and CC regulates cell bioenergetics, cytoplasmic calcium signals and CC activation of cell death pathways (By similarity). CC {ECO:0000250|UniProtKB:F4I111}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Ca(2+)(in) = Ca(2+)(out); Xref=Rhea:RHEA:29671, CC ChEBI:CHEBI:29108; Evidence={ECO:0000250|UniProtKB:F4I111}; CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane CC {ECO:0000250|UniProtKB:F4I111}; Multi-pass membrane protein CC {ECO:0000255}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=1; CC Comment=A number of isoforms are produced. According to EST CC sequences.; CC Name=1; CC IsoId=Q8VYR0-1; Sequence=Displayed; CC -!- SIMILARITY: Belongs to the MCU (TC 1.A.77) family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAG50752.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AC079733; AAG50752.1; ALT_INIT; Genomic_DNA. DR EMBL; CP002684; AEE33441.1; -; Genomic_DNA. DR EMBL; CP002684; AEE33442.1; -; Genomic_DNA. DR EMBL; AY070082; AAL49777.1; -; mRNA. DR PIR; C96610; C96610. DR RefSeq; NP_176074.2; NM_104558.4. [Q8VYR0-1] DR RefSeq; NP_974043.1; NM_202314.3. [Q8VYR0-1] DR AlphaFoldDB; Q8VYR0; -. DR SMR; Q8VYR0; -. DR STRING; 3702.Q8VYR0; -. DR PaxDb; 3702-AT1G57610.1; -. DR ProteomicsDB; 238245; -. [Q8VYR0-1] DR EnsemblPlants; AT1G57610.1; AT1G57610.1; AT1G57610. [Q8VYR0-1] DR EnsemblPlants; AT1G57610.2; AT1G57610.2; AT1G57610. [Q8VYR0-1] DR GeneID; 842137; -. DR Gramene; AT1G57610.1; AT1G57610.1; AT1G57610. [Q8VYR0-1] DR Gramene; AT1G57610.2; AT1G57610.2; AT1G57610. [Q8VYR0-1] DR KEGG; ath:AT1G57610; -. DR Araport; AT1G57610; -. DR TAIR; AT1G57610; MCU2. DR eggNOG; KOG2966; Eukaryota. DR HOGENOM; CLU_066330_1_0_1; -. DR InParanoid; Q8VYR0; -. DR OMA; ILAHKQV; -. DR OrthoDB; 469277at2759; -. DR PhylomeDB; Q8VYR0; -. DR PRO; PR:Q8VYR0; -. DR Proteomes; UP000006548; Chromosome 1. DR ExpressionAtlas; Q8VYR0; baseline and differential. DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005262; F:calcium channel activity; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0051560; P:mitochondrial calcium ion homeostasis; IEA:InterPro. DR InterPro; IPR006769; MCU_C. DR InterPro; IPR039055; MCU_fam. DR PANTHER; PTHR13462; CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL; 1. DR PANTHER; PTHR13462:SF10; CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL; 1. DR Pfam; PF04678; MCU; 1. PE 2: Evidence at transcript level; KW Alternative splicing; Calcium; Calcium channel; Calcium transport; KW Ion channel; Ion transport; Membrane; Metal-binding; Mitochondrion; KW Mitochondrion inner membrane; Reference proteome; Transit peptide; KW Transmembrane; Transmembrane helix; Transport. FT TRANSIT 1..33 FT /note="Mitochondrion" FT /evidence="ECO:0000255" FT CHAIN 34..293 FT /note="Calcium uniporter protein 2, mitochondrial" FT /id="PRO_0000431374" FT TRANSMEM 168..188 FT /note="Helical; Name=1" FT /evidence="ECO:0000255" FT TRANSMEM 198..218 FT /note="Helical; Name=2" FT /evidence="ECO:0000255" FT MOTIF 193..201 FT /note="Selectivity filter" FT /evidence="ECO:0000250|UniProtKB:Q8NE86" FT BINDING 197 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /evidence="ECO:0000250|UniProtKB:Q8NE86" SQ SEQUENCE 293 AA; 33685 MW; 3F599D18C29038D7 CRC64; MWSVMGLVRR TAMSSTVNKA SPVRSLLGGF RCLNVESKEE DEKKDMTVLE AKKLMRLVNV EDMKKKLIGM GDKEMVTYTT LIEASQGLGI ARSLDEAHAF ARVLDDAGVI LIFRDKVYLH PHKVVDLIRK AVPLGLNPDD ELIREEFDKM RSMKEEIDVL AHQQVRKILW GGLGYSVVQI GIFVRLTFWE FSWDVMEPIT FFTTATGIIV GYAYFLMTSR DPTYQDFMKR LFLSRQRKLL KSHKFDAERF KELENKWKIT SCSSSSCHAN ASIRNRVGVD LDLEDSLQSH HRD //